Evaluation of the antimicrobial activity and cytotoxicity of different components of natural origin present in essential oils

Even though essential oils (EOs) have been used for therapeutic purposes, there is now a renewed interest in the antimicrobial properties of phytochemicals and EOs in particular. Their demonstrated low levels of induction of antimicrobial resistance make them interesting for bactericidal applications, though their complex composition makes it necessary to focus on the study of their main components to identify the most effective ones. Herein, the evaluation of the antimicrobial action of different molecules present in EOs against planktonic and biofilm-forming Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria was assessed. The bactericidal mechanisms of the different molecules, as well as their cytocompatibility, were also studied. Carvacrol, cinnamaldehyde, and thymol exhibit the highest in vitro antimicrobial activities against E. coli and S. aureus, with membrane disruption the bactericidal mechanism identified. The addition of those compounds (=0.5 mg/mL) hampers S. aureus biofilm formation and partially eliminates preformed biofilms. The subcytotoxic values of the tested EO molecules (0.015–0.090 mg/mL) are lower than the minimum inhibitory and bactericidal concentrations obtained for bacteria (0.2–0.5 mg/mL) but are higher than that obtained for chlorhexidine (0.004 mg/mL), indicating the reduced cytotoxicity of EOs. Therefore, carvacrol, cinnamaldehyde, and thymol are molecules contained in EOs that could be used against E. coli– and S. aureus–mediated infections without a potential induction of bactericidal resistance and with lower cell toxicity than the conventional widely used chlorhexidine

Adapting Traffic Simulation for Traffic Management: A Neural Network Approach

Static models and simulations are commonly used in urban traffic management but none feature a dynamic element for near real-time traffic control. This work presents an artificial neural network forecaster methodology applied to traffic flow condition prediction. The spatially distributed architecture uses life-long learning with a novel adaptive Artificial Neural Network based filter to detect and remove outliers from training data. The system has been designed to support traffic engineers in their decision making to react to traffic conditions before they get out of control. We performed experiments using feed-forward backpropagation, cascade-forward back-propagation, radial basis, and generalized regression Artificial Neural Networks for this purpose. Test results on actual data collected from the city of Leicester, UK, confirm our approach to deliver suitable forecasts

TipTrap: A Co-located Direct Manipulation Technique for Acoustically Levitated Content

Acoustic levitation has emerged as a promising approach for mid-air displays, by using multiple levitated particles as 3D voxels, cloth and thread props, or high-speed tracer particles, under the promise of creating 3D displays that users can see, hear and feel with their bare eyes, ears and hands. However, interaction with this mid-air content always occurred at a distance, since external objects in the display volume (e.g. user's hands) can disturb the acoustic fields and make the particles fall. This paper proposes TipTrap, a co-located direct manipulation technique for acoustically levitated particles. TipTrap leverages the reflection of ultrasound on the users' skin and employs a closed-loop system to create functional acoustic traps 2.1 mm below the fingertips, and addresses its 3 basic stages: selection, manipulation and deselection. We use Finite-Differences Time Domain (FDTD) simulations to explain the principles enabling TipTrap, and explore how finger reflections and user strategies influence the quality of the traps (e.g. approaching direction, orientation and tracking errors), and use these results to design our technique. We then implement the technique, characterizing its performance with a robotic hand setup and finish with an exploration of the ability of TipTrap to manipulate different types of levitated content

Examining the role of wine brand love on brand loyalty: a multi-country comparison

This study develops and tests a model through a multi-country study that considers consumer wine knowledge and wine experience, wine brand trust and wine brand satisfaction as antecedents of wine brand love, and wine brand loyalty as a consequence of wine brand love. Data were collected in five wine-producing countries (Australia, Chile, France, Mexico and Portugal) with a final sample of 3462 completed surveys. Hypotheses were tested with structural equation modeling and the findings confirm the importance of brand love as both a mediator and direct influence on brand loyalty for wine consumers. Furthermore, brand satisfaction was positively and significantly related to brand love. In addition, wine experience, rather than wine knowledge, positively influenced brand trust and satisfaction. Finally, results also identify differences between countries thereby providing insights into how companies should focus their marketing strategies internationally.info:eu-repo/semantics/acceptedVersio

Laser cooling of trapped ions: the influence of micromotion

Laser cooling of a single trapped ion in a Paul trap is discussed theoretically in the Lamb-Dicke limit, with full consideration of the time dependence of the trapping potential. Resulting mean kinetic energies are defined as time averages over one period of the micromotion and are compared with final temperatures expected from the laser cooling treatment with harmonic traps. For laser-atom detunings close to the micromotion frequency the results differ significantly from those expected for a harmonic trap potential. A physical interpretation is given and simple formulas are derived for the strong confinement case

Numerical simulations of the cosmic star formation history

The cosmic star formation history in Cold Dark Matter dominated cosmological scenarios is studied by means of hydrodynamical numerical simulations. In particular, we explore a low density model with a Lambda-term and two high density models with different power spectra, all of them being spatially flat. Our simulations employ a fully nonlinear N-body and Eulerian hydrodynamics algorithm with a model for star formation and supernovae feedback that depends on two phenomenological parameters determined in agreement with previous papers. We find a nearly constant star formation rate beyond z=1, and we discuss which facts may determine the decrease in the SFR from z=1 to the present epoch. The Lambda-term cosmology with realistic parameters for star formation and feedback best reproduces the observed star formation history.Comment: 10 pages, 5 figures. Accepted for publication in A&

Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste

Citation: Esquivel-Elizondo, S., Parameswaran, P., Delgado, A. G., Maldonado, J., Rittmann, B. E., & Krajmalnik-Brown, R. (2016). Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste. Archaea-an International Microbiological Journal, 10. doi:10.1155/2016/4089684Inhibition by ammonium at concentrations above 1000mgN/L is known to harm the methanogenesis phase of anaerobic digestion. We anaerobically digested swine waste and achieved steady state COD-removal efficiency of around 52% with no fatty-acid or H-2 accumulation. As the anaerobic microbial community adapted to the gradual increase of total ammonia-N (NH3 -N) from 890 +/- 295 to 2040 +/- 30 mg/L, the Bacterial and Archaeal communities became less diverse. Phylotypes most closely related to hydrogenotrophic Methanoculleus (36.4%) and Methanobrevibacter (11.6%), along with acetoclastic Methanosaeta (29.3%), became the most abundant Archaeal sequences during acclimation. This was accompanied by a sharp increase in the relative abundances of phylotypes most closely related to acetogens and fatty-acid producers (Clostridium, Coprococcus, and Sphaerochaeta) and syntrophic fatty-acid Bacteria (Syntrophomonas, Clostridium, Clostridiaceae species, and Cloacamonaceae species) that have metabolic capabilities for butyrate and propionate fermentation, as well as for reverse acetogenesis. Our results provide evidence countering a prevailing theory that acetoclastic methanogens are selectively inhibited when the total ammonia-N concentration is greater than similar to 1000 mgN/L. Instead, acetoclastic and hydrogenotrophic methanogens coexisted in the presence of total ammonia-N of similar to 2000 mgN/L by establishing syntrophic relationships with fatty-acid fermenters, as well as homoacetogens able to carry out forward and reverse acetogenesis

Neurologic phenotype of Schimke immuno-osseous dysplasia and neurodevelopmental expression of SMARCAL1

Schimke immuno-osseous dysplasia (OMIM 242900) is an uncommon autosomal-recessive multisystem disease caused by mutations in SMARCAL1 (swi/snf-related, matrix-associated, actin-dependent regulator of chromatin, subfamily a-like 1), a gene encoding a putative chromatin remodeling protein. Neurologic manifestations identified to date relate to enhanced atherosclerosis and cerebrovascular disease. Based on a clinical survey, we determined that half of Schimke immuno-osseous dysplasia patients have a small head circumference, and 15% have social, language, motor, or cognitive abnormalities. Postmortem examination of 2 Schimke immuno-osseous dysplasia patients showed low brain weights and subtle brain histologic abnormalities suggestive of perturbed neuron-glial migration such as heterotopia, irregular cortical thickness, incomplete gyral formation, and poor definition of cortical layers. We found that SMARCAL1 is highly expressed in the developing and adult mouse and human brain, including neural precursors and neuronal lineage cells. These observations suggest that SMARCAL1 deficiency may influence brain development and function in addition to its previously recognized effect on cerebral circulation