Regional vitiligo induced by imiquimod treatment for in-transit melanoma metastases

Imiquimod is a topical immunomodulator used for the treatment of viral warts and superficial basal cell carcinoma and as an emerging therapy for lentigo maligna and cutaneous melanoma metastases. Vitiligo-like depigmentation has been described as a local adverse effect of topical imiquimod therapy for melanoma1 and at distant sites in patients treated with combination topical monobenzone-imiquimod for metastatic melanoma.2 We present a case in which topical imiquimod resulted in both the resolution of in-transit metastatic melanoma and the depigmentation of local and regional skin, suggesting a regional lymphatic effect of this localized topical treatment

Critical implications of IVDR for innovation in diagnostics: input from the BioMed alliance diagnostics task force

With the implementation of Regulation (European Union [EU]) 2017/746 on in vitro diagnostic medical devices (IVDR), from May 26, 2022, onwards, the development and use of diagnostic tests will be governed by a vastly expanded and upgraded EU regulatory framework. We provide here an overview of the amended transition timelines, the role of notified bodies, EU reference laboratories, expert panels, and the Medical Device Coordination Group (MDCG). We also describe the implications of the IVDR for innovative laboratory medicine by explaining the exemption for in-house devices (IH-IVDs). Two key challenges faced by the academic diagnostic sector are: (1) the stipulation on equivalence of tests (article 5.5d), which poses a new condition for the use of IH-IVDs and (2) the gray area between CE marked in vitro diagnostics (CE-IVDs), modified CE-IVDs, Research Use Only (RUO) tests, and IH-IVDs. Furthermore, the results of a questionnaire on current diagnostic practice conducted by European medical societies collaborating in the BioMed Alliance indicate widespread use of IH-IVDs in diagnostic laboratories across Europe and emphasize the need for support and guidance to comply with the IVDR. Diagnostic equivalents of the European Reference Networks (ERNs) for rare diseases could help ensure affordable and equal access to specialized diagnostics across the EU. Concerted action by clinical and laboratory disciplines, regulators, industry, and patient organizations is needed to support the efficient and effective implementation of the IVDR in a way that preserves innovation and safeguards the quality, safety, and accessibility of innovative diagnostics.Peer reviewe

2018 Research & Innovation Day Program

A one day showcase of applied research, social innovation, scholarship projects and activities.https://first.fanshawec.ca/cri_cripublications/1005/thumbnail.jp

Adult-onset bilateral Parry-Romberg syndrome

Parry-Romberg Syndrome (PRS), or progressive hemifacial atrophy, is an uncommon disorder characterized by progressive unilateral loss of adipose tissue and underlying structures including muscle, cartilage, and bone, often with little or no sclerosis. PRS and morphea en coup de sabre (ECDS) have significant overlap, often coexist, and are likely different phenotypes of morphea.1 PRS usually presents in the first decade of life, but later presentations have been described.1 It is more common in females and the pathogenesis is not completely understood.1 Neurologic symptoms are the most common extracutaneous systemic manifestation. Bilateral disease occurs in rare instances.1 We describe a woman with profound bilateral facial atrophy whose presentation does not follow the typically reported disease course or histopathologic findings seen in PRS

Prepared by Titan Systems

This evaluation study reports the results of research and analysis undertaken by the U.S. Censu

Antibiotic resistome profile of Subject A is rapidly remodeled immediately after FMT.

<p>Antibiotic resistance gene abundances were measured by alignment to the CARD antibiotic resistance database [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182585#pone.0182585.ref019" target="_blank">19</a>] and normalizing by per-sample coverage. Individual gene abundances are aggregated by antibiotic class. Genes conferring multiple antibiotic class resistance phenotypes are counted toward each antibiotic class.</p

Genus-level taxonomic composition reveals extensive short-term donor microbial engraftment with FMT, followed by long-term reduction in donor similarity relative to donor self-similarity over a similar time period.

<p>Genera present in the donor appear in (a) Subject A and (b) Subject B recipients with inconsistent long-term residence. For visual clarity, genera represented by >5% of assigned reads from at least one timepoint are shown. (c) Whole-community Bray-Curtis donor similarity for Subjects A and B, and an additional four patients (Subjects C, D, E and F) for which long-term and donor samples were obtained, is shown for genus-level composition. Donor similarity is calculated for each recipient timepoint with the corresponding donor sample used for FMT. As a point of reference, we show multiple timepoints for Donor #29, where similarity is measured against the first timepoint. Microbial sequencing reads were classified using Kraken [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182585#pone.0182585.ref029" target="_blank">29</a>] in conjunction with a sequence database collected from NCBI Refseq and Genbank microbial genome references. (d) Whole-community donor Chao similarity [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182585#pone.0182585.ref033" target="_blank">33</a>] in gene content is shown. Gene abundances were measured by alignment to the Uniref50 functionally annotated protein sequence database [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182585#pone.0182585.ref032" target="_blank">32</a>].</p

Diamondoid Coating Enables Disruptive Approach for Chemical and Magnetic Imaging with 10 nm Spatial Resolution

Diamondoids are unique molecular nano-materials with diamond structure and fascinating properties such as negative electron affinity and short electron mean free paths. A thin layer of diamondoids deposited on a cathode is able to act as an electron monochromator, reducing the energy spread of photo-emitted electrons from a surface. This property can be applied effectively to improve the spatial resolution in x-ray photoemission electron microscopy (X-PEEM), which is limited by chromatic aberration of the electron optics. In this paper, we present X-PEEM measurements reaching the technological relevant spatial resolution of 10?nm without the need of expensive and complex corrective optics. Our results provide a simple approach to image surface chemical and magnetic information at nanometer scales by employing diamondoids