Etude De Quelques Proprietes Biologiques De Ocimum Gratissimum L., Une Lamiaceae Recoltee A Daloa (Côte d'Ivoire)

Introduction: Ocimum gratissimum is used in traditional Ivorian medicine for its multiple therapeutic virtues. Scope: Evaluate the biological properties of the essential oil of this plant. Methods: Antitumor activity was assessed using the MTS colorimetric assay on six (06) cell lines, the antifungal activity was evaluated by double dilution slant method on four (04) strains of clinical fungi, the potential antioxidant was determined by the DPPH test. Results: The strongest cytotoxic activity was obtained on Jurkat (T lymphoma) with an IC50 = 80 μg / ml. The best antifungal activity was observed on C. albicans with MIC and FMC values obtained at 12.5 μg / ml. Antioxidant activity was evaluated at F = 187 ± 1.57 mM Trolox / ml or (I = 38 ± 0.74%). Conclusion: The essential oil has interesting biological properties like as cytotoxic, antifungal and antioxidan

Evaluation De La Qualité Microbiologique Des Médicaments Traditionnels Améliorés (MTA) Vendus Dans Six Communes d’Abidjan (Côte d’Ivoire)

This study was carried out in six (6) communes of Abidjan within a period of six months (November 2016 to April 2017). This paper focuses on assessing the microbiological quality of traditional medicines produced and sold in these communes. A total of 38 samples were collected, including 19 in powder form and another 19 in liquid form. The search and enumeration of the germs was carried out according to the method based on standard procedures (AFNOR). Enumeration included total mesophilic aerobic flora, total coliforms, yeasts and molds, Staphylococcus aureus, and salmonella. The results indicate an absence of the pathogenic flora (Salmonella and S. aureus). There is also an absence of yeasts and molds in the products in liquid form. The level of contamination of the Mesophilic Total Aerobic Flora (FMAT) ranges from 1.104 to 6.7.107 CFU / ml or CFU / g and from 1.6.104 to 5.5.107 CFU / g or CFU / ml for total coliforms. In terms of microbiological quality, 39.47% of the analyzed samples had compliant. In view of these results, it is necessary to train Traditional Medicine Practitioners on Good Manufacturing Practices (GMP) methods throughout the production chain in order to market MTAs of guaranteed quality and safet

Évaluation de l’activité antibactérienne des extraits de feuilles de Solanum torvum Swartz (Solanaceae) sur la croissance in vitro de 3 souches d’entérobactéries

Objectif: Établir des bases scientifiques de l’action antibactérienne de Solanum torvum Swartz (Solanaceae), une plante médicinale utilisée en médecine traditionnelle contre les infections bactériennes.Méthodologie et Résultats: Les extraits issus de cette plante ont été testés sur la croissance in vitro des souches de E. coli, de K. pneumoniae et de Salmonella sp en milieu liquide et solide de Mueller-Hinton. Les résultats ont révélé que ces extraits ont une activité antibactérienne dose-dépendante sur ces souches bactériennes utilisées. Cependant, l’extrait acétonique 100% a un meilleur potentiel antibactérien sur la souche de E. coli (CMI = 6,25 mg/mL et CMB = 1,563 mg/mL) que sur c el l e d e Salmonella sp (CMI = 3,125 mg/mL et CMB = 6,25 mg/mL) et de K. pneumoniae (CMI = 6,25 mg/mL et CMB = 12,5 mg/mL).Conclusion et application des résultats: Cet extrait peut être utilisé pour la mise au point de phytomédicaments contre les gastroentérites à E. coli et à Salmonella sp.Mots clés: Antibactérienne, extraits, Solanum torvum, in vitro, gastroentéritesEnglish AbstractObjective: Establish scientific bases of the antibacterial action of Solanum torvum Swartz (Solanaceae), a medicinal plant used in traditional medicine against the bacterial infections.Methodology and Results: The extracts resulting from this plant were tested on in vitro growth of the stocks of E. coli, K. pneumoniae and Salmonella sp in Mueller-Hinton broth and Mueller-Hinton naehrboden agar. The results have revealed that these extracts have dose-dependent action against bacteria targeted. However, the 100% acetonic extract has a better antibacterial potential on the stock of E. coli (MIC = 6.25 mg/mL and MBC = 1.563 mg/mL) than on Salmonella sp (MIC = 3.125 mg/mL and MBC = 6.25 mg/mL) and of K. pneumoniae (MIC = 6.25 mg/mL and MBC = 12.5 mg/mL).Conclusion and application of the results: This extract can be used to formulate a drug against the gastroenteritis with E. coli and Salmonella sp.Keywords: Antibacterial, extracts, Solanum torvum, in vitro, gastroenteriti

Total Phenolic Compounds Extraction in Leaves of Ocimum gratissimum L. and Their Potential Activity against Some Agricultural Contaminants

Aims: Phenolic compounds are secondary metabolites that are important in the plant due to their role in plant defense and their antioxidant activity with other biological properties such as antipyretic, analgesic and antimicrobial activities. This study focused on the biological potential activity of total phenolic compounds extracted by soxhlet method from Ocimum gratissimum leaves (Lamiaceae), a popular medicinal plant harvested at Daloa (Côte d’Ivoire). Study Design: Activities were directed on the in vitro antifungal and antioxidant activities combined to phenolic compounds analysis. Place and Duration of Study: The study was carried out at the Department of Environment and Plant Protection, and Laboratory of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine (USAMV) between March to July 2014, Laboratory of Biochemistry and Microbiology (Bioactives Natural Substances Unit), Jean Lorougnon Guédé University between September 2014 to January 2015. Methodology: The extract obtained named TPCOG was tested against Fusarium species for its antifungal activity by applying agar slant double dilution method and for its antioxidant activity by DPPH radical scavenging assay. Qualitative and quantitative evaluation of phenolic compounds were carried out by HPLC analysis method with sigma chemical standards. Results: Tests showed that TPCOG was a powerful antifungal extract with MIC and MFC ranging between 3.125 µg/mL to 12.5 µg/mL. This extract was fungicidal and its antioxidant activity reached F= 541.25± .25 mM Trolox/mL (I= 70 ±1.85%) with a total phenolic content equal 195.70±1.33 mg GAE/g. Radical scavenge and antifungal activities correlated very well with total phenolic compounds. Phenolic content analysis showed presence of phenolic acids and flavonoids with a higher concentration for Quercetin (393.475 mg/100 g sample). Conclusion: All this results demonstrated the biological potential activity of total phenolic compounds extracted from leaves of O. gratissimum and the possibility to use it in replace to essential oil for the formulation of biofungicides and nutraceuticals by industries

Evaluation de la toxicité d’un aphrodisiaque ivoirien d’origine naturelle (aphro) chez le rat

L’usage des plantes à des fins thérapeutiques est très ancien. De nos jours encore, elles sont utilisées par une frange partie de la population mondiale. La présente étude avait pour objectif d’étudier les effets toxiques de l'administration subaiguë d'un phytomédicament ivoirien Aphro vendu sur les marchés chez le rat Wistar albinos. Pour ce faire, le phytomédicament Aphro a été administré quotidiennement par voie orale à trois lots de rats, aux doses de 5,75 ; 11,5 et 23 mg/kg de poids corporel et cela pendant 28 jours. Le taux de croissance des rats, les paramètres hématologiques et biochimiques sanguins ont été étudiés. Les résultats de cette étude ont révélé qu’aux doses de 5,75 ; 11,5 et 23 mg/kg de poids corporel, le phytomédicament ne modifie pas de façon significative le taux d’accroissement de la masse corporelle et les paramètres hématologiques constitués du taux de globules rouges, de globules blancs, de plaquettes sanguines et le taux d’hémoglobine. Par ailleurs, des marqueurs hépatiques et lipidiques sériques des rats traités n'ont pas été perturbés par l’administration du phytomédicament comparativement au lot témoin. En revanche, la créatinine, l’un des marqueurs rénaux étudiés a connu une augmentation significative (p < 0,05) chez les rats traités avec le phytomédicament aux doses de 11,5 et 23 mg/kg de poids corporel par rapport au lot témoin. Cette étude a permis de montrer que l’usage prolongé et à doses élevées de Aphro pourrait entraîner des effets nocifs sur les reins.   English title: Assessment of the toxicity of an ivoirian natural aphrodisiac (aphro) on rat The use of plants for therapeutic purposes is very old. Even today, they are used by a part of the world population. The objective of the present study was to evaluate the toxic effects of the subacute administration of an ivorian phytomedicine Aphro sold in the markets in the albino Wistar rat. To do this, the phytomedicine Aphro was administered orally daily to three batches of rats, at doses of 5.75; 11.5 and 23 mg/kg of body weight for 28 days. Rats' growth rate, blood haematological and biochemical parameters were studied. The results of this study showed that at doses of 5.75; 11.5 and 23 mg/kg of body weight, the phytomedicine does not significantly modify the rate of increase in body mass and the hematological parameters consisting of the level of red blood cells, white blood cells, blood platelets and the level hemoglobin. Furthermore, hepatic and serum lipid markers of the treated rats were not disturbed by the administration of the phytomedicine compared to the control group. In contrast, creatinine, one of the renal markers studied, experienced a significant increase (p <0.05) in rats treated with the phytomedicine at doses of 11.5 and 23 mg / kg of body weight compared to the batch witness. This study showed that prolonged use and in high doses of Aphro could cause harmful effects on the kidneys

Comparative Study of the Activity of Citrus Sinensis Barks on the In Vitro Growth of Candida Albicans and Trichophyton Rubrum

<p>ABSTRACT</p><p>The emergence as well as resistance of pathogenic microorganisms currently poses a particularly serious public health problem. The use of medicinal plants is therefore one of the most interesting avenues to explore. It is with this in mind that this work was carried out and aims to evaluate the activity of <i>citrus sinensis</i> bark on the growth of <i>Candida albicans</i> and <i>Trichophyton rubrum</i>. In this study, we proceeded to the hydroethanolic extraction at 70% of the bark of this plant and the extract was tested by the method of double dilution. The results showed that the two microbial strains are sensitive to the ethanolic extract of <i>Citrus sinensis</i> according to the dose-response relationship with an MIC value of 50mg/mL for <i>Candida albicans</i> and an MIC value greater than 50mg/mL for <i>Trichophyton rubrum</i>. The extract is fungistatic against<i> Candida albicans </i>but has weak inhibitory activity for <i>Trichophyton rubrum</i>.</p><p><strong>Keywords</strong>: Antifungal activity- <i>Citrus sinensis</i> - mycosis</p><p> </p&gt