Multi-Focal, Multi-Centric Angiosarcoma of Bone

A multi-focal multi-centric, malignant tumour of vascular origin arising in bone in a 55-year-old man is described. The presenting symptoms were pain and weight loss. Radiologically, multiple lytic lesions were demonstrated in the long bones of both legs and throughout the pelvis. Histological examination demonstrated an angiosarcoma which was predominantly low grade in nature but with focal areas of intermediate grade. Turnout cells expressed the endothelial markers CD31, CD34 and von Willebrand's factor. There was rapid radiological progression of disease with no response to radiotherapy. Pain abated within a few days of institution of doxorubicin, 75 mg m-2, but the patient died of massive pulmonary thromboembolism 14 days later, 11 months after the first symptoms

The mediterranean dietary pattern and breast cancer risk in Greek-Cypriot women: a case-control study

Background: Diet has long been suspected to impact on breast cancer risk. In this study we evaluated whether the degree of adherence to a Mediterranean diet pattern modifies breast cancer risk amongst Greek-Cypriot women. Methods: Subjects included 935 cases and 817 controls, all participating in the MASTOS case-control study in Cyprus. The study was approved by the Cyprus National Bioethics Committee. Information on dietary intakes was collected using an interviewer administered 32-item Food Frequency Questionnaire. Information on demographic, anthropometric, lifestyle, and other confounding factors was also collected. Adherence to the Mediterranean Diet pattern was assessed using two a-priory defined diet scores. In addition, dietary patterns specific to our population were derived using Principal Component Analysis (PCA). Logistic regression models were used to assess the association between the dietary patters and breast cancer risk. Results: There was no association with breast cancer risk for either score, however, higher consumptions of vegetables, fish and olive oil, were independently associated with decreased risk. In addition, the PCA derived component which included vegetables, fruit, fish and legumes was shown to significantly reduce risk of breast cancer (ORs across quartiles of increasing levels of consumption: 0.89 95%CI: 0.65-1.22, 0.64 95%CI: 0.47-0.88, 0.67 95%CI: 0.49-0.92, P trend < 0.0001), even after adjustment for relevant confounders. Conclusions: Our results suggest that adherence to a diet pattern rich in vegetables, fish, legumes and olive oil may favorably influence the risk of breast cancer. This study is the first investigation of dietary effects on breast cancer risk in Cyprus, a country whose population has traditionally adhered to the Mediterranean diet

An investigation of breast cancer risk factors in Cyprus: a case control study

Background: Breast cancer is the most common form of malignancy affecting women worldwide. It is also the leading cancer in females in Cyprus, with approximately 400 new cases diagnosed annually. It is well recognized that genetic variation as well as environmental factors modulate breast cancer risk. The main aim of this study was to assess the strength of associations between recognized risk factors and breast cancer among Cypriot women. This is the first epidemiological investigation on risk factors of breast cancer among the Cypriot female population.Methods: We carried out a case-control study, involving 1,109 breast cancer patients and a group of 1,177 controls who were recruited while participating in the National screening programme for breast cancer. Information on demographic characteristics and potential risk factors were collected from both groups during a standardized interview. Logistic regression analysis was used to assess the strength of the association between each risk factor and breast cancer risk, before and after adjusting for the possible confounding effect of other factors.Results: In multivariable models, family history of breast cancer (OR 1.64, 95% CI 1.23, 2.19) was the strongest predictor of breast cancer risk in the Cypriot population. Late menarche (OR 0.64, 95% CI 0.45, 0.92 among women reaching menarche after the age of 15 vs. before the age of 12) and breastfeeding (OR 0.74, 95% CI 0.59, 0.92) exhibited a strong protective effect. In the case of breastfeeding, the observed effect appeared stronger than the effect of pregnancy alone. Surprisingly, we also observed an inverse association between hormone replacement therapy (HRT) although this may be a product of the retrospective nature of this study.Conclusion: Overall the findings of our study corroborate with the results of previous investigations on descriptive epidemiology of risk factors for breast cancer. This investigation provides important background information for designing detailed studies that aim to improve our understanding of the epidemiology of breast cancer in the Cypriot population, including the study of gene-environment interactions. Furthermore, our study provides the first scientific evidence for formulating targeted campaigns for prevention and early diagnosis of breast cancer in Cyprus

Αξιολόγηση κλινικοεργαστηριακής παρακολούθησης, ψυχικής υγείας, και ποιότητας ζωής εφήβων και νέων γυναικών με σύνδρομο Turner

Σκοπός: Το σύνδρομο Turner (ΣΤ) σχετίζεται με μια ποικιλία νοσημάτων που επηρεάζουν σχεδόν κάθε σύστημα του σώματος και απαιτεί πολυεπιστημονική διαχείριση. Ο ερευνητικός σκοπός αυτής της μελέτης είναι να διερευνηθεί κατά πόσον η κλινικοεργαστηριακή παρακολούθηση των ατόμων με ΣΤ είναι επαρκής και σύμφωνη με τις διεθνείς προτεινόμενες κατευθυντήριες ιατρικές οδηγίες. Επιπλέον, να ελεγχθούν η ψυχική κατάσταση και η ποιότητα ζωής(ΠΖ) των ατόμων αυτών, ώστε να διαπιστωθεί εάν το σύνδρομο τα επηρεάζει αρνητικά. Υλικό και Μέθοδος: Στην έρευνα έλαβαν μέρος 19 γυναίκες ηλικίας 15-61 ετών διαγνωσμένες με ΣΤ. Το δείγμα λήφθηκε από την ερευνητική ομάδα της διπλωματικής εργασίας στο ΓΝΑ Αλεξάνδρα κατά τα έτη 2015-2018. Οι γυναίκες με ΣΤ εντοπίστηκαν από βάσεις δεδομένων του νοσοκομείου, και τα δεδομένα εξήχθησαν από τα αρχεία τους και συγκρίθηκαν με τις προτεινόμενες οδηγίες. Για τον έλεγχο ψυχικής υγείας και ποιότητας ζωής χρησιμοποιήθηκαν ψυχομετρικά εργαλεία-ερωτηματολόγια (WHOQOL-BREF, HADS, STAI, DASS-21). Παράλληλα, τα ίδια ερωτηματολόγια χορηγήθηκαν σε δείγμα 30 υγιών γυναικών, ηλικίας 15-50 ετών, που αντιπροσωπεύουν την ομάδα ελέγχου με φυσιολογικό καρυότυπο. Η στατιστική επεξεργασία των δεδομένων έγινε με το πρόγραμμα SPSS v.23 σε συνδυασμό με το Microsoft Excel. Αποτελέσματα: Το μέσο ύψος και βάρος των συμμετεχόντων ήταν 150 ± 6,48 cm και 58 ± 9,59 kg αντίστοιχα. Το 75% των ασθενών με καταγεγραμμένα δεδομένα είχαν υποβληθεί σε θεραπεία με GH πριν από την ηλικία των 13 ετών, και μόλις το 8% είχαν πρόκληση ήβης σε ηλικία 11-12 ετών. Μόνο σε 11% των ασθενών είχε πραγματοποιηθεί ακοολογική εκτίμηση με ακοόγραμμα ανά 5ετία, καθώς και οδοντιατρική/ορθοδοντική εκτίμηση. 83% από αυτές είχαν υποβληθεί σε τουλάχιστον ένα διαθωρακικό υπερηχογράφημα καρδιάς ή αξονική τομογραφία/ μαγνητική τομογραφία καρδιάς. Το 33% είχε ετήσια αξιολόγηση της αρτηριακής πίεσης, ενώ στο 63% είχε αξιολογηθεί η μέτρηση οστικής πυκνότητας (DXA) και στο 74% νευροψυχολογική/συμπεριφορική κατάσταση. Η λειτουργία του θυρεοειδούς εξετάστηκε ετησίως στο 42% των γυναικών, η HbA1c +/- Gl στο 44%, το λιπιδαιμικό προφίλ στο 63% και η ηπατική λειτουργία στο 47%. Τα επίπεδα ορού της 25-ΟΗ-VitD μετρήθηκαν κάθε 2-3 χρόνια σε 47% των ασθενών, και έγινε έλεγχος για αντισώματα κοιλιοκάκης στο 32% των ασθενών. Κατά την εκτίμηση της ψυχικής υγείας και της ΠΖ με τη χορήγηση των ερωτηματολογίων παρατηρήθηκαν στατιστικά σημαντικές διαφορές στα επίπεδα του στρες, του άγχους και της κατάθλιψης, του χαρακτηριολογικού άγχους, της συνολικής ΠΖ καθώς και των διαστάσεων της σωματικής και ψυχολογικής υγείας και του περιβάλλοντος, όπου οι ασθενείς με ΣΤ είχαν χαμηλότερη βαθμολογία στην ψυχική υγεία (στρες, άγχος, κατάθλιψη, χαρακτηριολογικό άγχος) και υψηλότερη βαθμολογία στην ΠΖ σε σύγκριση με την ομάδα του υγιούς πληθυσμού. Επίσης, παρατηρήθηκε στατιστικά σημαντική και αρνητική συσχέτιση (r=-0,536, p=0,018) μεταξύ ηλικίας και χαρακτηριολογικού άγχους. Η διερεύνηση της επίδρασης της οικογενειακής κατάστασης, του εκπαιδευτικού επιπέδου, της επαγγελματικής απασχόλησης και του γονότυπου δεν έδειξε στατιστικά σημαντικό αποτέλεσμα (p&gt;0,05). Συμπεράσματα: Ένα σημαντικό ποσοστό γυναικών με ΣΤ φαίνεται ότι δεν υποβάλλεται σε όλους τους αναγκαίους διεθνείς προτεινόμενους κλινικοεργαστηριακούς ελέγχους. Αυτό τονίζει την ανάγκη για βελτίωση της παρεχόμενης κλινικής φροντίδας, ώστε οι ασθενείς να παρακινούνται και να εμπλέκονται ενεργά στη διατήρηση της δια βίου παρακολούθησής τους, προκειμένου να βελτιστοποιηθεί η ποιότητα ζωής τους και να μειωθούν οι συννοσηρότητές τους. Ωστόσο παρατηρείται ότι η ψυχική τους υγεία και η συνολική ποιότητα ζωής δεν επηρεάζονται σημαντικά λόγω του ΣΤ.Objective: Turner Syndrome (TS) is associated with a variety of morbidities affecting nearly every organ and system, and necessitates multidisciplinary management. The research purpose of this study is to investigate whether the clinical and laboratory monitoring of women with TS is adequate and in accordance with international medical guidelines. In addition, to assess the mental health and quality of life of these women, to determine if the syndrome affects them negatively. Material and Method: The study involved 19 women, aged 15-61 years, diagnosed with TS. The study population consisted of patients’ data retrieved from their medical records in “GNA Alexandra” during the years 2015-2018. Psychometric tools-questionnaires (WHOQOL-BREF, HADS, STAI, DASS-21) were used for collection of additional data. Simultaneously, the same questionnaires were given to a random sample of 30 women, aged 15-50 years, representing the healthy population with a normal karyotype. Statistical analysis was carried out using SPSS v.25 along with Microsoft Excel. The women with TS were identified from hospital databases, and the data were extracted from their records and compared with the proposed international recommendations. Psychometric tools-questionnaires (WHOQOL-BREF, HADS, STAI, DASS-21) were used to assess mental health and quality of life. At the same time, these questionnaires were also given to a sample of healthy women, aged 15-50 years, representing the control group with a normal karyotype. The statistical processing of the data was done with the program SPSS v.23 in combination with Microsoft Excel. Results: The average height and weight of the identified participants was 150 ± 6.48 cm and 58± 9.59 kg respectively. 75% of the patients with reported data had been treated with GH treatment before the age of 13, and just 8% had induction of puberty at age 11-12 years. Only 11% of patients had had a 5-yearly audiometric evaluation and a dental/orthodontic evaluation. 83% of them had undergone at least one transthoracic echocardiography or CT/cardiac magnetic resonance scan. 33% had an annual assessment of blood pressure, while 63% had been evaluated by a dual energy x-ray absorptiometry (DXA) scan and 74% had a neuropsychological/ behavioural assessment. Thyroid function was annually screened in 42% of the women, HbA1c +/- Gl in 44%, lipid profile in 63% and liver function in 47%. Serum 25-OH-VitD levels were measured every 2-3 years in 47%, and a screening for coeliac disease antibodies had been performed in 32% of the patients. The assessment of mental health and quality of life with the given questionnaires showed statistically significant differences in the levels of stress, anxiety and depression, characteristic anxiety, total quality of life as well as the dimensions of physical and psychological health and the environment, where patients had a lower score on mental health (stress, anxiety, depression, characteristic anxiety) and a higher score on quality of life compared to the healthy control group. There was also a statistically significant and negative correlation (r = -0.536, p = 0.018) between age and characteristic stress. The investigation of the effect of marital status, educational level, professional employment, and genotype did not show a statistically significant difference (p&gt; 0.05). Conclusions: A significant proportion of TS women may miss health checks proposed by the international guidelines. This highlights the need for a better care plan strategy to engage and motivate these women for lifelong follow-up, in order to optimise their quality of life and reduce their comorbidities. However, their mental health and overall quality of life are not significantly affected by TS

Φυτοχημική ανάλυση και βιολογική δράση επιλεγμένων φαρμακευτικών και αρωματικών φυτών (Drimia numidica, Smilax aspera, Rosmarinus officinalis, Cannabis sativa L., Crocus sativus L.) στην αντιμετώπιση του σακχαρώδη διαβήτη και του καρκίνου

Medicinal and aromatic plants are widely used for their health promoting properties. Given the fact that the benefits from their use has been established through many in vitro and in vivo studies, it is a reasonable assumption that medicinal and aromatic plants will play a crucial role for human health in the future.In this thesis, the chemical profile and the in vitro and in vivo bioactivity of Drimia numidica, Smilax aspera L, Rosmarinus officinalis L, Crocus sativus L. and cannabidiol, the non-psychotropic compound of Cannabis sativa were studied. The chemical profile of the extracts was determined with the use of liquid chromatography combined with time-of-flight mass spectrometry while bioactivity of the extracts in terms of antioxidant activity was evaluated with the DPPH and ABTS assays. Estimation of the total phenolic content of the plant material was performed with the Folin Ciocalteu assay. Given the rich chemical profile of the extracts in secondary metabolites, followed by their strong or even moderate antioxidant activity, in vitro experiments regarding their cytotoxic activity as possible anticancer agents against brain malignances were performed. In these experiments it was also investigated the cytotoxic activity of dimethylcrocetin, a semi-natural derivate from the stigmas of Crocus sativus L. Medulloblastoma (TE671) and glioblastoma (A172) cancer cell lines were used and cytotoxicity was monitored at 24, 48 and 72h after treatment with the chemicals using the Alamar blue assay. Results showed that the plant material under study as well as the single compounds used, namely dimethylcrocetin and cannabidiol, inhibit cancer cell growth and proliferation in a dose and time dependent manner. In vivo experiments included the use of Crocus sativus L and specifically the use of crocins, the most abundant constituent of saffron stigmas. Experiments were performed using zebrafish as an experimental model. It was estimated whether crocins can regulate glucose levels of larvae zebrafish. A significant decrease of glucose levels was observed on the treated embryos; therefore, it was further investigated whether expression of pck1, the gene that regulates the rate limiting stem of gluconeogenesis is influenced by crocins. According to RT-PCR results, pck1 was significantly upregulated respect to the control treated embryos, indicating that reduction of glucose was not via inhibition of the gluconeogenesis pathway. However, since pancreatic β-cells secrete insulin to regulate glucose metabolism, it was examined if CRCs affect β-cells formation of developing zebrafish embryos. The transgenic line Tg(ins:DsRed) was used and results showed that treated embryos with the extract of crocins promoted insulin expression, as it was observed by the increased fluorescence intensity derived from the pancreatic islets. This was further confirmed by investigating the expression of insa. Insa was significantly upregulated compared to the control group, supporting the implication of the extract in glucose homeostasis.Η χρήση των φαρμακευτικών και αρωματικών φυτών είναι σήμερα ευρέως διαδεδομένη λόγω των πολλαπλών ωφελειών τους στην προαγωγή της καλής υγείας. Τα οφέλη από τη χρήση τους έχουν επιβεβαιωθεί μέσα από in vitro και in vivo μελέτες, επομένως γίνεται αντιληπτό πως τα φαρμακευτικά και αρωματικά φυτά θα συνεχίζουν να διαδραματίζουν καθοριστικό ρόλο ως σημαντικοί παράγοντες με σκοπό την προάσπιση της υγείας.Στην παρούσα διδακτορική διατριβή μελετήθηκε η χημική σύσταση καθώς και η in vitro και in vivo βιοδραστικότητα εκχυλισμάτων προερχόμενων από τα φυτά Drimia numidica, Smilax aspera L, Rosmarinus officinalis L, Cannabis sativa L., Crocus sativus L., καθώς και της κανναβιδιόλης, της μη ψυχότροπης ουσίας που προέρχεται από το φυτό της Cannabis sativa. Το χημικό προφίλ των εκχυλισμάτων προσδιορίστηκε με τη μέθοδο της υγρής χρωματογραφίας συζευγμένης με φασματομετρία μάζας χρόνου πτήσης, ενώ η εκτίμηση της βιοδραστικότητας των εκχυλισμάτων ως προς την αντιοξειδωτική τους ικανότητα έγινε με τις δοκιμασίες DPPH και ABTS. Η εκτίμηση του ολικού φαινολικού περιεχομένου πραγματοποιήθηκε με τη μέθοδο Folin-Ciocalteu.Δεδομένου του πλούσιου χημικού προφίλ των εκχυλισμάτων σε δευτερογενείς μεταβολίτες και σε συνδυασμό με την ισχυρή ή ακόμη και μέτρια αντιοξειδωτική δράση τους, έγιναν in vitro πειράματα που αφορούσαν στη διερεύνηση της κυτταροτοξικής τους δράσης σε κακοήθεις όγκους εγκεφάλου. Σε αυτή τη σειρά των πειραμάτων εξετάστηκε και η κυτταροτοξική δράση της διμεθυλοκροκετίνης, ενός ημι-φυσικού παραγώγου προερχόμενου από τα στίγματα του Crocus sativus L. Εξετάστηκαν δυο κυτταρικές σειρές και συγκεκριμένα η σειρά ΤΕ671 (κύτταρα μυελοβλαστώματος) και η σειρά Α172 (κύτταρα γλοιοβλαστώματος). Η δράση των εκχυλισμάτων παρακολουθήθηκε σε τρεις χρονικές στιγμές, δηλαδή στις 24, 48 και 72h, χρησιμοποιώντας την τεχνική Alamar blue. Τα αποτελέσματα έδειξαν ότι το φυτικό υλικό που μελετήθηκε καθώς και οι καθαρές ουσίες, διμεθυλοκροκετίνη και κανναβιδιόλη, αναστέλλουν την ανάπτυξη και τον πολλαπλασιασμό των καρκινικών κυττάρων με μία δόσο- και χρόνο- εξαρτώμενη σχέση. Στα in vivo πειράματα, μελετήθηκε η δράση εκχυλίσματος κροκινών οι οποίες εκχυλίστηκαν από τα στίγματα του φυτού Crocus sativus L. Τα πειράματα πραγματοποιήθηκαν χρησιμοποιώντας ως πειραματικό μοντέλο το zebrafish, και εκτιμήθηκε εάν οι κροκίνες μπορούν να ρυθμίσουν τα επίπεδα γλυκόζης στο zebrafish. Παρατηρήθηκε σημαντική μείωση των επιπέδων της γλυκόζης στις προνύμφες όπου χορηγήθηκαν κροκίνες. Ως εκ τούτου διερευνήθηκε η έκφραση του γονιδίου pck1, το οποίο ρυθμίζει τη διαδικασία της γλυκονεογένεσης. Τα αποτελέσματα έδειξαν σημαντική αύξηση έκφρασης του γονιδίου σε σχέση με τα έμβρυα στα οποία δεν χορηγήθηκαν κροκίνες, γεγονός που υποδηλώνει ότι η μείωση της γλυκόζης που παρατηρήθηκε, δεν είναι αποτέλεσμα παρεμπόδισης της γλυκονεογένεσης. Στη συνέχεια εξετάστηκε εάν οι κροκίνες επηρεάζουν το σχηματισμό των β-κυττάρων σε προνύμφες zebrafish. Χρησιμοποιήθηκε η διαγονιδιακή σειρά Tg(ins:DsRed) και τα αποτελέσματα έδειξαν πως οι κροκίνες ενεργοποίησαν την παραγωγή ινσουλίνης, όπως παρατηρήθηκε από την αυξημένη ένταση φθορισμού των παγκρεατικών νησιδίων, το οποίο επιβεβαιώθηκε μελετώντας και την έκφραση του γονιδίου insa. Παρατηρήθηκε υπερέκφραση του συγκεκριμένου γονιδίου σε σχέση με τα έμβρυα στα οποία δεν είχαν χορηγηθεί κροκίνες, γεγονός που οδηγεί στο συμπέρασμα ότι οι κροκίνες συμμετέχουν στη διαδικασία ομοιόστασης της γλυκόζης

Study on the Chemical Composition, Enzyme Inhibition and Antioxidant Activity of Ziziphora taurica subsp. cleonioides

Ziziphora is a plant used in Turkish and Iran traditional medicine for its antibacterial activity, sedative and stomach soothing properties. Although the chemical profile of the essential oil of different Ziziphora species is well documented, data regarding plant extracts are incomplete. In this study extracts from Ziziphora taurica subsp. cleonioides were obtained using ethyl acetate, methanol and water and the chemical profile of the aerial part of the plant was elucidated. Among the compounds identified, rosmarinic acid was the most abundant (3375.67 +/- 38.02 mu g/mL), at the extract of methanol, followed by chlorogenic acid (3225.10 +/- 16.44). Enzyme inhibition activity against alpha-amylase and tyrosinase was also estimated. The ethyl acetate extract showed the highest alpha-amylase activity (1.95 +/- 0.04 mg/mL), while the best anti-tyrosinase activity was calculated for the methanolic extract (1.25 +/- 0.01 mg/mL). In addition, total phenolic, flavonoid content and antioxidant activity were evaluated. According to our results, bioactivity of the plant is of great interest, nonetheless, at the same time, it is strongly depended on the solvent used during the extraction process. Our data suggest that the plant under study may be an important source to consider against metabolic, skin pigmentation and oxidative stress related disorders

Five Years of Spatially Resolved Ground-Based MAX-DOAS Measurements of Nitrogen Dioxide in the Urban Area of Athens: Synergies with In Situ Measurements and Model Simulations

Long-term nitrogen dioxide (NO2) slant column density measurements using the MAX-DOAS (multi-axis differential optical absorption spectroscopy) technique were analyzed in order to demonstrate the temporal and horizontal variability of the trace gas in Athens for the period October 2012–July 2017. The synergy with in situ measurements and model simulations was exploited for verifying the MAX-DOAS technique and its ability to assess the spatiotemporal characteristics of NO2 pollution in the city. Tropospheric NO2 columns derived from ground-based MAX-DOAS observations in two horizontal and five vertical viewing directions were compared with in situ chemiluminescence measurements representative of urban, urban background and suburban conditions; a satisfactory correlation was found for the urban (r ≈ 0.55) and remote areas (r ≈ 0.40). Mean tropospheric slant columns retrieved from measurements at the lowest elevation over the urban area ranged from 0.1 to 32 × 1016 molec cm−2. The interannual variability showed a rate of increase of 0.3 × 1016 molec cm−2 per year since 2012 in the urban area, leading to a total increase of 20%. The retrieved annual cycles captured the seasonal variability with lower NO2 levels in summer, highly correlated (r ≈ 0.85) with the urban background and suburban in situ observations. The NO2 diurnal variation for different seasons exhibited varied patterns, indicating the different role of photochemistry and anthropogenic activities in the different seasons. Compared to in situ observations, the MAX-DOAS NO2 morning peak occurred with a one-hour delay and decayed less steeply in winter. Measurements at different elevation angles are shown as a primary indicator of the vertical distribution of NO2 at the urban environment; the vertical convection of the polluted air masses and the enhanced NO2 near-surface concentrations are demonstrated by this analysis. The inhomogeneity of the NO2 spatial distribution was shown using a relevant inhomogeneity index; greater variability was found during the summer period. Comparisons with city-scale model simulations demonstrated that the horizontal light path length of MAX-DOAS covered a distance of 15 km. An estimation of urban sources’ contribution was also made by applying two simple methodologies on the MAX-DOAS measurements. The results were compared to NO2 predictions from the high resolution air quality model to infer the importance of vehicle emissions for the urban NO2 levels; 20–35% of the urban NO2 was found to be associated with road transport

Chemical Characterization and Antioxidant Activity of Nine <i>Hypericum</i> Species from Greece

Hypericum L. comprises about 500 species distributed almost worldwide. Research has mainly focused on H. perforatum with confirmed biological activity on the alleviation of depression symptoms, among others. The compounds responsible for such activity are considered naphthodianthrones and acylphloroglucinols. Other Hypericum species are less studied or not studied, and further research is needed to complete the characterization of the genus. In this study we evaluated the qualitative and quantitative phytochemical profile of nine Hypericum species native to Greece, namely H. perforatum, H. tetrapterum, H. perfoliatum, H. rumeliacum subsp. apollinis, H. vesiculosum, H. cycladicum, H. fragile, H. olympicum and H. delphicum. Qualitative analysis was performed using the LC/Q-TOF/HRMS technique, while quantitative data were calculated with the single point external standard method. Additionally, we estimated the antioxidant activity of the extracts using DPPH and ABTS assays. Three species endemic to Greece (H. cycladicum, H. fragile, H. delphicum) were studied for the first time. Our results indicated that all studied species are rich in secondary metabolites, mainly of the flavonoids family, with strong antioxidant activity