Doxorubicin pharmacokinetics in lymphoma patients treated with doxorubicin-loaded eythrocytes

Doxorubicin-loaded erythrocytes (DLE) were administrated to 15 lymphoma patients. Antibiotic peak concentration in blood decreased by 55%, doxorubicin circulated several times longer, and the area under the concentration-time curve increased 5 times if compared with standard doxorubicin administration. The DLE was well tolerated by patients

Chemical and biological indicators for evaluation of Arctic soil degradation and its potential to remediation

In recent years, significant efforts have been made to accelerate the economic development of the Arctic zone, leading to intense environmental pollution of this region, accompanied by the significant impact of accumulated environmental damage in the region. The solution to these problems is difficult due to the remoteness of these areas and severe climatic conditions. Therefore, it is important to evaluate the potential for restoration of arctic soils. For this purpose, various indicators are used, including biological ones. In the analyzed arctic soil samples, high concentrations of petroleum hydrocarbons (up to 47,000 mg/kg) and chloride-ions (0.10–0.14 wt %) were established. Microbioassay demonstrated a presence of hydrocarbon-oxidizing microorganisms: Penicillium, Azotobacter chroococcum, Bacillus subtilis, Pseudomonas oleovorans. A low enzymatic activity and specific Arctic climate point out a low self-restoration ability of the soil, demonstrated the need for its remediation. The microbioassay with microbial strains identification and soil remediation methods suitable for the Arctic zone were recommended

Характеристика основных показателей периферической крови у подростков в Томской области

Background. The adolescent period is characterized by the features of growth and development of the organism when the risk of a number of diseases increases under the conditions of hormonal adjustment and under the influence of environmental factors. Objective. Our aim was to determine the reference values of the main values of peripheral blood and the prevalence of anemia in adolescents residing in the territory of the Tomsk region. Methods. We studied the results of medical examination of children aged from 13 to 16 years. To determine the main values of the general blood test (erythrocytes, hemoglobin, leukocytes, erythrocyte sedimentation rate), an automatic hematological analyzer was used. Evaluation of physical development was carried out by standard methods. The analysis of the results was carried out considering the sex, living conditions, weight, height, and body mass index. Results. We analyzed the values of peripheral blood of 7,120 adolescents. Rated values corresponded to the conventional age norms. The hemoglobin level and the number of erythrocytes are associated with the body weight, height, and body mass index of the respondents. Anemia of varying severity was recorded in 618/7,120 (8.7%) adolescents. More often anemia was verified in rural areas (OR 1.52; CI 95% 1.28–1.80) as well as in females (OR 2.62; CI 95% 2.18–3.15). Conclusion. Regional features of peripheral blood values and the prevalence of anemia in adolescents of the Tomsk region have been determined. Подростковый период характеризуется особенностями роста и развития организма, когда в условиях гормональной перестройки под воздействием факторов внешней среды увеличивается риск формирования ряда заболеваний. Цель исследования — определить референсные значения основных показателей периферической крови и распространенность анемии у подростков, проживающих на территории Томской области. Методы. Изучали результаты диспансеризации детей в возрасте от 13 до 16 лет. Для определения основных показателей общего анализа крови (эритроциты, гемоглобин, лейкоциты, скорость оседания эритроцитов) использовался автоматический гематологический анализатор. Оценка физического развития проводилась стандартными методами. Анализ результатов проводили с учетом пола, условий проживания, веса, роста и индекса массы тела. Результаты. Проанализированы показатели периферической крови 7120 подростков. Значения показателей соответствовали условным возрастным нормам. Уровень гемоглобина и количество эритроцитов ассоциированы с массой тела, ростом и индексом массы тела респондентов. Анемия различной степени тяжести была зарегистрирована у 618/7120 (8,7%) под-ростков. Чаще анемию верифицировали у жителей сельских районов (OR 1,52; CI 95% 1,28–1,80), а также у лиц женского пола (OR 2,62; CI 95% 2,18–3,15). Заключение. Установлены региональные особенности показателей периферической крови и степень распространенности анемии у подростков Томской области.

Половое развитие подростков в Томской области

Background. Sexual development of adolescents is one of the important indicators of the population well-being, the study of which, in the context of the world trend towards a change in the period of puberty, can contribute to the identification of public health problems. Objective. Our aim was to study the basic indicators of sexual development of adolescents and to determine the regional characteristics of this population. Methods. The evaluation of secondary sexual characteristics was carried out by specialists according to Tanner criteria. Ultrasound examination of organs of the reproductive system was carried out using a portable scanner Mindray M7. The analysis of the results was carried out taking into account the gender, living conditions, physical development. Results. We examined 7,120 adolescents aged from 13 to 16 years. The degree of axillary hair distribution by Tanner criteria was 2.4±0.7 in young men, 2.9±0.4 — in girls, pubic hair — 2.0±0.9 and 2.6±0.8, respectively. The indicator of development of mammary glands by Tanner criteria in girls was 2.6±0.8, menstrual function by Tanner criteria — 2.4±0.8. Herewith, urban adolescents had later terms of appearance of the secondary sexual characteristics in comparison with the inhabitants of rural areas. Conclusion. We registered the delayed sexual development in 8.1% of girls and 13.6% of young men. Urban youths have experienced a delay in gonadal growth. Among the female population there was an outstripping growth and development of the uterus and ovaries in comparison with rural adolescents. Половое развитие подростков — один из важных показателей благополучия населения, изучение которого в условиях мировой тенденции к изменению сроков наступления пубертатного периода может способствовать выявлению проблем общественного здравоохранения. Цель исследования — изучить основные показатели полового развития подростков и определить региональные особенности данной популяции. Методы. Оценка вторичных половых признаков проводилась врачами-специалистами по критериям Tanner. Ультразвуковое исследование органов репродуктивной системы осуществлялось при помощи переносного сканера Mindray M7. Анализ результатов проводили с учетом пола, условий проживания, физического развития. Результаты. Обследовано 7120 подростков в возрасте от 13 до 16 лет. Степень оволосения подмышечной впадины по критериям Tanner составила у юношей 2,4±0,7, у девушек — 2,9±0,4, степень лобкового оволосения — 2,0±0,9 и 2,6±0,8 соответственно. Показатель развития молочных желез по критериям Tanner у девушек составил 2,6±0,8, менструальная функция по критериям Tanner — 2,4±0,8. При этом у городских подростков выявлены более поздние сроки появления вторичных половых признаков в сравнении с жителями сельских районов. Заключение. У 8,1% девушек и 13,6% юношей зарегистрирована задержка полового развития. У городских юношей выявлена задержка роста гонад; среди женского населения наблюдалось опережение роста и развития матки и яичников в сравнении с сельскими подростками.

Genetic landscape in Russian patients with familial left ventricular noncompaction

BackgroundLeft ventricular noncompaction (LVNC) cardiomyopathy is a disorder that can be complicated by heart failure, arrhythmias, thromboembolism, and sudden cardiac death. The aim of this study is to clarify the genetic landscape of LVNC in a large cohort of well-phenotyped Russian patients with LVNC, including 48 families (n=214).MethodsAll index patients underwent clinical examination and genetic analysis, as well as family members who agreed to participate in the clinical study and/or in the genetic testing. The genetic testing included next generation sequencing and genetic classification according to ACMG guidelines.ResultsA total of 55 alleles of 54 pathogenic and likely pathogenic variants in 24 genes were identified, with the largest number in the MYH7 and TTN genes. A significant proportion of variants −8 of 54 (14.8%) −have not been described earlier in other populations and may be specific to LVNC patients in Russia. In LVNC patients, the presence of each subsequent variant is associated with increased odds of having more severe LVNC subtypes than isolated LVNC with preserved ejection fraction. The corresponding odds ratio is 2.77 (1.37 −7.37; p <0.001) per variant after adjustment for sex, age, and family.ConclusionOverall, the genetic analysis of LVNC patients, accompanied by cardiomyopathy-related family history analysis, resulted in a high diagnostic yield of 89.6%. These results suggest that genetic screening should be applied to the diagnosis and prognosis of LVNC patients

Robust Packing Patterns And Luminescence Quenching In Mononuclear [Cu(Ii)(Phen)2] Sulfates

Three mixed-ligand Cu(II) complexes with compositions [Cu(phen)2(SO4)]·CH3OH (1), [Cu(phen)2(SO4)](H2O)2(dmf) (2), and [Cu(phen)2H2O](SO4)(H2O)4 (3), where phen = 1,10-phenanthroline and dmf = N,Nβ-dimethylformamide, were prepared and studied. These compounds belong to the landscape of the mononuclear Cu(phen)2 sulfates, and the solvated complexes undergo frequent anion/water exchange at the metal center in aqueous solutions. Complexes are similar by the metal trigonal bipyramidal coordination geometry but differ by the mode of enclathration and number of protic and aprotic solvent guest molecules being accommodated in the crystal lattice. Crystal packing in 1-3 is determined by the robust supramolecular patterns that consist of stacking interactions between the planar extended phen fragments. These are observed in all three solids regardless of the interplay of other noncovalent interactions, including rather strong hydrogen bonds. The dual luminescence is detected at 580 and 470 nm for both crystals of phen and 3. Detailed analysis of singlet and triplet excitations in phen and 3 is performed by time-dependent density functional methods. Fluorescence is predicted with a low quantum yield at 386 nm, and dual phosphorescence from n-∗ and -∗ triplet states is predicted at 523 and 496 nm. Emission quenching was demonstrated for 3 and explained by nonradiative decay involving supramolecular stacking and low-lying metal-centered states

PREVENTION OF NIPPLE CRACKS OF THE MAMMARY GLAND IN THE EARLY POSTNATAL PERIOD

Preservation and prolongation of the lactation period is not only a guarantee of the child's full physical and mental development but also one of the most important methods for reducing the risk of developing breast cancer. Problems with the mammary gland nipple in a woman in the early postnatal period lead to a refusal of lactation. We carried out a retrospective analysis (period from 2010 to 2016) of the causes of traumatizing mammary gland nipples in the early postnatal period in 172 women (mean age 29.1 ± 4.3 years). Methods of prevention and treatment of nipple injuries in the early postnatal period have been offered for the lactation period prolongation

Thermodynamic Hydricity of Small Borane Clusters and Polyhedral closo-Boranes

Thermodynamic hydricity (HDAMeCN) determined as Gibbs free energy (ΔG°[H]−) of the H− detachment reaction in acetonitrile (MeCN) was assessed for 144 small borane clusters (up to 5 boron atoms), polyhedral closo-boranes dianions [BnHn]2−, and their lithium salts Li2[BnHn] (n = 5–17) by DFT method [M06/6-311++G(d,p)] taking into account non-specific solvent effect (SMD model). Thermodynamic hydricity values of diborane B2H6 (HDAMeCN = 82.1 kcal/mol) and its dianion [B2H6]2− (HDAMeCN = 40.9 kcal/mol for Li2[B2H6]) can be selected as border points for the range of borane clusters’ reactivity. Borane clusters with HDAMeCN below 41 kcal/mol are strong hydride donors capable of reducing CO2 (HDAMeCN = 44 kcal/mol for HCO2−), whereas those with HDAMeCN over 82 kcal/mol, predominately neutral boranes, are weak hydride donors and less prone to hydride transfer than to proton transfer (e.g., B2H6, B4H10, B5H11, etc.). The HDAMeCN values of closo-boranes are found to directly depend on the coordination number of the boron atom from which hydride detachment and stabilization of quasi-borinium cation takes place. In general, the larger the coordination number (CN) of a boron atom, the lower the value of HDAMeCN

Characteristics of the Main Indicators of Peripheral Blood in Adolescents in the Tomsk Region

Background. The adolescent period is characterized by the features of growth and development of the organism when the risk of a number of diseases increases under the conditions of hormonal adjustment and under the influence of environmental factors. Objective. Our aim was to determine the reference values of the main values of peripheral blood and the prevalence of anemia in adolescents residing in the territory of the Tomsk region. Methods. We studied the results of medical examination of children aged from 13 to 16 years. To determine the main values of the general blood test (erythrocytes, hemoglobin, leukocytes, erythrocyte sedimentation rate), an automatic hematological analyzer was used. Evaluation of physical development was carried out by standard methods. The analysis of the results was carried out considering the sex, living conditions, weight, height, and body mass index. Results. We analyzed the values of peripheral blood of 7,120 adolescents. Rated values corresponded to the conventional age norms. The hemoglobin level and the number of erythrocytes are associated with the body weight, height, and body mass index of the respondents. Anemia of varying severity was recorded in 618/7,120 (8.7%) adolescents. More often anemia was verified in rural areas (OR 1.52; CI 95% 1.28–1.80) as well as in females (OR 2.62; CI 95% 2.18–3.15). Conclusion. Regional features of peripheral blood values and the prevalence of anemia in adolescents of the Tomsk region have been determined