Presynchronization of estrous cycles in dairy cows before ovsynch + CIDR and resynchronization of repeat estrus using the CIDR

Postpartum anestrus is one of the major limitations to achieving acceptable pregnancy rates. The Ovsynch protocol is an excellent tool to improve reproductive efficiency of dairy cows because it can induce estrous cycles in anestrous cows. In the first experiment, administering two PGF2 injections to lactating dairy cows 14 days apart with the second injection given 12 days before the Ovsynch protocol increased (P\u3c0.05) pregnancy rate by 10 percentage points in cycling and noncycling cows. Inserting a progesterone-releasing insert (CIDR) for 7 days during the Ovsynch protocol did not further increase pregnancy rates. In a second experiment, a resynchronization treatment consisting of a used CIDR inserted for 7 days from days 13 to 20 after insemination increased (P\u3c0.05) embryo survival from day 30 to 58 by 11 percentage points but failed to increase overall rate of return to estrus and conception rate at the second AI (first eligible estrus after first AI).; Dairy Day, 2001, Kansas State University, Manhattan, KS, 2001

Effect of sire, age at first calving, season and year of calving and parity on reproductive performance of Friesian cows under semiarid conditions in Egypt.

SUMMARY The objective of this paper was to study some factors affecting reproductive performance of a locally-born Friesian herd in Egypt. Data of 2096 reproductive records representing 482 Friesian cows daughters of 38 sires raised at the Dairy Unit of Milk and Meat Project of Faculty of Agriculture, Alexandria University, Egypt (located at the northern western part of the Nile Delta) between 1985-2002 were utilized to study the effects of sire of the cow, age at first calving (AFC), season and year of calving/birth and parity on period from parturition to first service (FSP), days open (DO), calving interval (CI), number of services per conception (NSC), AFC and breeding efficiency (BE). The least squares mixed model analysis by SAS (1999) indicated that the overall least squares means (± standard error) of FSP, DO, CI, NSC, AFC and BE were 88.4 ± 1.1, 130.7 ± 1.9, 403.1 ± 1.9 days, 2.1 ± 0.1 services, 30.7 ± 0.1 months and 90.1 ± 0.6 %, respectively. Sire had highly significant (P<0.01) effect on DO, CI, NSC and AFC, but had insignificant effect on FSP and BE. The influence of AFC on FSP was highly significant (P<0.01) and not significant on DO CI, NSC and BE. Cows had AFC more than 36 months had the longest FSP (92.6±4.5) and cows had AFC less than 29 months of age had 87.8±2.7 days FSP. Season of calving had significant effect on FSP (P<0.01), DO and CI (P<0.05), but had insignificant effect on NSC. Season of birth had no significant effect on both AFC and BE. Cows calving in autumn had the shortest CI (394.3±4.7 days) comparing with those calved in other seasons (ranged between 404.8±6.5 and 409.4±5.2 days). Cows calving in autumn also had the shortest DO (122.6±4.8 days). Year of calving/birth had significant (P<0.05 or P<0.01) effect on all traits studied. A decreasing efficiency in reproductive performance of cows was observed over time. The shortest FSP and DO were in the period 1988 to 1990 (64.3±5.3 and 98.1±8.9 days, respectively) and the longest were in the period 2000 to 2002 (107.5±5.2 and 188.1±8.8 days, respectively). Calving interval increased from 371 days in [1988][1989][1990] to 450 days † Corresponding autho

Presynchronization of estrous cycles in dairy cows before ovsynch + CIDR and resynchronization of repeat estrus using the CIDR

Postpartum anestrus is one of the major limitations to achieving acceptable pregnancy rates. The Ovsynch protocol is an excellent tool to improve reproductive efficiency of dairy cows because it can induce estrous cycles in anestrous cows. In the first experiment, administering two PGF2 injections to lactating dairy cows 14 days apart with the second injection given 12 days before the Ovsynch protocol increased (P\u3c0.05) pregnancy rate by 10 percentage points in cycling and noncycling cows. Inserting a progesterone-releasing insert (CIDR) for 7 days during the Ovsynch protocol did not further increase pregnancy rates. In a second experiment, a resynchronization treatment consisting of a used CIDR inserted for 7 days from days 13 to 20 after insemination increased (P\u3c0.05) embryo survival from day 30 to 58 by 11 percentage points but failed to increase overall rate of return to estrus and conception rate at the second AI (first eligible estrus after first AI).; Dairy Day, 2001, Kansas State University, Manhattan, KS, 2001

Fixed-time artificial insemination in beef cattle

which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background: The study was designed to test the effect of fixed-time artificial insemination (fixed-AI) after the slightly modified Ovsynch protocol on the pregnancy rate in beef cattle in Finnish field conditions. The modification was aimed to optimize the number of offsprings per AI dose. Methods: Ninety Charolais cows and heifers were entered into the program an average of 1.8 times. Thus, 164 animal cases were included. Animals were administered 10-12 μg of buserelin. Seven days later animals without a corpus luteum (CL) were rejected (20.7%) while the remaining 130 cases with a CL were administered prostaglandin F2α, followed 48 h later with a second injection of buserelin (8-10 μg). Fixed-AI was performed 16-20 hours after the last injection. Results: The pregnancy rate was 51.5 % (67/130). The pregnancy rate after a short interval (50-70 d) from calving to entering the program was significantly higher than that after a long interval (>70 d). Conclusion: This protocol seems to give acceptable pregnancy results in beef herds and its effect on saving labour is notable

Use of stochastic simulation to evaluate the reduction in methane emissions and improvement in reproductive efficiency from routine hormonal interventions in dairy herds

This study predicts the magnitude and between herd variation in changes of methane emissions and production efficiency associated with interventions to improve reproductive efficiency in dairy cows. Data for 10,000 herds of 200 cows were simulated. Probability of conception was predicted daily from the start of the study (parturition) for each cow up to day 300 of lactation. Four scenarios of differing first insemination management were simulated for each herd using the same theoretical cows: A baseline scenario based on breeding from observed oestrus only, synchronisation of oestrus for pre-set first insemination using 2 methods, and a regime using prostaglandin treatments followed by first insemination to observed oestrus. Cows that did not conceive to first insemination were re-inseminated following detection of oestrus. For cows that conceived, gestation length was 280 days with cessation of milking 60 days before calving. Those cows not pregnant after 300 days of lactation were culled and replaced by a heifer. Daily milk yield was calculated for 730 days from the start of the study for each cow. Change in mean reproductive and economic outputs were summarised for each herd following the 3 interventions. For each scenario, methane emissions were determined by daily forage dry matter intake, forage quality, and cow replacement risk. Linear regression was used to summarise relationships. In some circumstances improvement in reproductive efficiency using the programmes investigated was associated with reduced cost and methane emissions compared to reliance on detection of oestrus. Efficiency of oestrus detection and the time to commencement of breeding after calving influenced variability in changes in cost and methane emissions. For an average UK herd this was a saving of at least £50 per cow and a 3.6% reduction in methane emissions per L of milk when timing of first insemination was pre-set

Efficacy of ovulation synchronization with timed artificial insemination in treatment of follicular cysts in dairy cows

The efficacy of five ovulation-synchronization protocols with FTAI in treatment of cows diagnosed with follicular cysts was investigated in a nonrandomized study in a single herd. Cows identified with follicular structures >25 mm on two subsequent ultrasonographic (USG) examinations 7–10 days apart (n = 552) were assigned to one of the five treatment regimens on the day of the second USG examination. Treatment regimens were Ovsynch (GnRH-7d-PGF2α-56h-GnRH-16h-FTAI), New-CIDR (as Ovsynch with a new CIDR insert being fitted between days 0 and 7), Reused-CIDR (as New-CIDR but the CIDR insert was previously used for 7 days in another cow), G-New CIDR (Pre-GnRH on day 0 with the New-CIDR regimen being initiated 7 days later), and RG-Ovsynch (Pre-GnRH on day 0 and then every 7 days until detection of a luteal structure upon USG examination; at that point the Ovsynch was initiated). A subset of cows was subjected to ovarian USG examination at the time of PGF2α administration, at insemination, and 8–10 days post-insemination. Progesterone-releasing ability of new and reused CIDR inserts were evaluated in cows diagnosed with severe ovarian inactivity (n = 16). The data were analyzed using logistic regression with pregnancy per AI on days 30 (P/Al 30) and 70 (P/AI 70) post-insemination were included as outcome measures. Compared with Ovsynch, RG-Ovsynch improved the P/AI 30 (OR = 2.6, P = 0.03) and the P/AI 70 (OR = 2.5, P = 0.05). New-CIDR and G-New CIDR were associated with non-significant increase in P/AI 30 (OR = 2.1, P = 0.09 and OR = 2.3, P = 0.07, respectively) and P/AI 70 (OR = 2.01, P = 0.09 and OR = 2.2, P = 0.09, respectively). Reused-CIDR was not associated with improvement in P/AI (P = 0.93 and 0.79 for P/AI 30 and P/AI 70, respectively). RG-Ovsynch had a longer diagnosis-to-FTAI interval (median 24, IQR 17,31). The dominant ovarian structures and the presence of a cyst or a luteal structure at PGF2α administration or at insemination were not associated with P/AI. The new and re-used CIDR inserts produced comparable concentrations of serum progesterone 3 h, 3 days and 7 days post CIDR insertion. In conclusion, the RG-Ovsynch improved the P/AI whereas the New-CIDR and the G-New CIDR regimens tended to increase the P/AI compared with Ovsynch. Marginal differences in P/AI between RG-Ovsynch, New-CIDR, and G-New-CIDR together with prolonged diagnosis-to-FTAI in RG-Ovsynch should be considered if to evaluate the economic value of these regimens

Supplemental progesterone increases pregnancy rates and embryo survival in lactating dairy cows

Administering progesterone to lactating dairy cows has sometimes proven effective in increasing pregnancy rates. In this study, cows were treated with the Ovsynch protocol in addition to supplemental progesterone given for 7 days between the first gonadotropin- releasing hormone (GnRH) injection and the prostaglandin F2á (PGF2á) injection. Conception rates were greater in lactating Holstein dairy cows receiving exogenous progesterone (62.5%) than in controls treated with only the Ovsynch protocol (35.5%). In addition, progesterone supplementation increased embryo survival between 28 and 56 days of pregnancy