Isolation And molecular characterization of xylella fastidiosa from different host plant species in Italy (Apulia region)

L'isolement en culture pure de la souche Xylella fastidiosa liée au syndrome de déclin rapide de l'olivier (OQDS), récemment observé dans les Pouilles (sud de l'Italie) a été tenté à partir des plantes symptomatiques naturellement infectée, principalement Olea europaea L., Polygala myrtifolia et Rosmarinus officinalis, en utilisant les méthodes d'impression et d'extraction de la sève. Avant l'isolement, RT-LAMP et qPCR ont été utilisés pour déterminer la présence de X. fastidiosa chez tous les hôtes. Des cultures bactériennes pures ont été obtenues à partir d'extraits d'Olea europaea L. et de Polygala myrtifolia étalés dans un milieu tamponné d'extrait de cystéine-levure (BCYE). Deux isolats d'olive ont ensuite été typés à l'aide du système de séquençage multilocus (MLST). Les résultats indiquent qu'Olea europaea a un grand potentiel d'isolement, et la méthode d'impression a présenté des résultats nettement meilleurs. MLST montre des points communs génétiques avec la souche De Donno (ST53) et confirme que nous avons toujours le même type de séquence ST53 dans la région.Pure culture isolation of the Xylella fastidiosa strain related to the olive quick declinesyndrome (OQDS), lately noticed in Apulia (southern Italy) was attempted fromnaturally infected symptomatic plants mainly Olea europaea L., Polygala myrtifolia and Rosmarinus officinalis using printing and sap extraction methods. Prior to isolation, RTLAMP and qPCR were used to determine the presence of Xylella fastidiosa in all hosts. Pure bacterial cultures were obtained from both Olea europaea L. and Polygala myrtifolia extracts plated in buffered cysteine-yeast extract (BCYE) media. Two olive isolates were subsequently typed using the Multilocus Sequencing System (MLST). Findings indicate that beside Olea europaea, Polygala myrtifolia seems to have a good potential for isolation, and printing method has presented significantly better results. MLST shows genetic commonality with De Donno (ST53) strain and ascertain that Apulia region still have the same sequence type ST53 in the region

Reduction of post-harvest injuries caused by Drosophila suzukii in some cultivars of sweet cherries using a high carbon dioxide level and cold storage

9openInternationalItalian coauthor/editorEfficient strategies are required in sweet cherry fruits to control the spotted wing drosophila (SWD), Drosophila suzukii, due to its adverse economic effect on farmers. Cold storage (CS) and storage with elevated carbon dioxide (CO2) are environmentally safe approaches for the pest control of stored fresh fruit. These strategies are effective in controlling a wide variety of insect species, without allowing toxic compounds to accumulate. The purpose of this study was to assess the effectiveness of a post-harvest application of CO2 treatment at 50%, cold treatment at 4 °C (CT), and a combination of both (CO2-CT) in controlling the early stages of SWD within four cultivars of freshly harvested cherry fruit, namely “Burlat-Bigarreau”, “Giorgia”, “Ferrovia”, and “Lapins”. In addition, an evaluation of the quality attributes of the cherries (skin firmness, berry firmness, strong soluble material, and titratable acidity) was carried out at harvest and after 10 and 20 days of storage. All treatments significantly reduced the rate of emergence of SWD when compared to the control (untreated cherry at 24 °C), and 100% SWD mortality was obtained in Burlat-Bigarreau (CO2-CT). In addition, over the entire storage time, the quality parameters were preserved in the samples stored at 4 °C and in the samples with combined treatments in comparison with the control.openMostafa, Manal; Ibn Amor, Abir; Admane, Naouel; Anfora, Gianfranco; Bubici, Giovanni; Verrastro, Vincenzo; Scarano, Luciano; El Moujabber, Maroun; Baser, NurayMostafa, M.; Ibn Amor, A.; Admane, N.; Anfora, G.; Bubici, G.; Verrastro, V.; Scarano, L.; El Moujabber, M.; Baser, N

Landscape restoration due to Xylella fastidiosa invasion in Italy: Assessing the hypothetical public’s preferences

Since 2013, the olive landscapes have gradually degenerated due to the spread and establishment of Xylella fastidiosa subsp. pauca (hereafter Xf) in Apulia, southern Italy. From 2013 to 2019, a total of approximately 54,000 hectares of olive orchards in the south of this region have been seriously damaged, and their restoration will progressively regenerate the economic, social, cultural and environmental nonmarket benefits. Since there is a willingness to restore the affected landscape in the best interest of the local citizens, this research aims to predict their preference heterogeneity and willingness to pay (WTP) to improve this landscape and continue research and experimentation in relation to Olive Quick Decline Syndrome Disease by the bacterium. For this purpose, a choice experiment method is used. The social field survey includes a representative sample of 683 respondents in three major cities (Foggia, Bari and Lecce) of Apulia region. The results reveal that for the local citizens interviewed, the most appreciated olive landscape services are cultural heritage and aesthetic values. In addition, the findings revealed citizens’ positive appreciation of improving the damaged olive landscape, while respondents are not willing to pay a premium for research. The results show that the average value that Apulians are willing to pay for landscape restoration is about 5.7 million of € per year. Further, this research has implications for land use planners in the study area, which faces issues of harmful pathogen management and land revival

Xylella fastidiosa invasion of new countries in Europe, the Middle East and North Africa: Ranking the potential exposure scenarios

After the recent high-impact European outbreaks of Xylella fastidiosa (Xf), a xylem-limited plant pathogenic bacterium native to the Americas, this research aims to rank the risks of potential entry, establishment and spread of Xf in new countries across Europe, the Middle East and North Africa. A novel risk-ranking technique is developed, based on combining entry risk drivers (imported plants, direct flights and ferry connections) with risk factors related to establishment and spread (presence of potential insect vectors, vulnerable economic crops, alternative hosts and climate suitability) of this pathogen. This reveals that western European countries have the highest risk for entry, but that the Mediterranean basin runs the highest risk for establishment and spread of Xf. Lebanon in particular has the highest level of risk for Xf dispersal within its suitable territory. Countries without current outbreaks combining high risks of Xf arrival and establishment are mainly in the Mediterranean basin: Turkey is at the highest level of risk, followed by Greece, Morocco and Tunisia, which are ranked at the high level. The ranking model also confirms the vulnerability, in terms of invasion by Xf, of southern European countries (Italy, Portugal and Spain) in which the pathogen has already been reported. High summer temperatures in these southern countries are likely to be the significant determinant for the overall invasion process, while northern European countries have a high level risk for the arrival of the pathogen, but relatively low summer temperatures may limit establishment and spread of major outbreaks. In general, our study provides a useful approach for mapping and comparing risks of invasive non-native species and emerging pathogens between countries, which could be useful for regional horizon scanning and phytosanitary and biosecurity management

Drought, climate change and sustainability of water in agriculture: A roadmap towards the NWRS2

The frequency and intensity of drought, extreme events and high wind velocities in South Africa are expected to increase in the next century as a result of the changing climate. The National Water Resource Strategy 2 (NWRS2) has set out the general and strategic directions for water resources management in the country for the next 20 years. However, the strategy does not draw a framework tailored specifically for agricultural use, with specific measures and goals. Therefore, to reach sustainability of water in agriculture, four major strategic goals are suggested, on which research institutions can focus and promote through good governance. The strategy emphasises: (1) crop research to find new drought-and heat- tolerant and resistant breeds and varieties; (2) intensified research in agricultural practices; (3) increasing the efficiency of water use within agriculture; and (4) integrating all these strategic goals within a sustainable research framework. Finally, the research calls for rapid action and implementation. Significance:  The framework is proposed for stakeholders and policymakers in higher education, agriculture and resources management in South Africa for new research horizons at national level to improve overall agricultural sustainability by 2030 as stipulated by the Millennium Development Goals

Drought, climate change and sustainability of water in agriculture: A roadmap towards the NWRS2

The frequency and intensity of drought, extreme events and high wind velocities in South Africa are expected to increase in the next century as a result of the changing climate. The National Water Resource Strategy 2 (NWRS2) has set out the general and strategic directions for water resources management in the country for the next 20 years. However, the strategy does not draw a framework tailored specifically for agricultural use, with specific measures and goals. Therefore, to reach sustainability of water in agriculture, four major strategic goals are suggested, on which research institutions can focus and promote through good governance. The strategy emphasises: (1) crop research to find new drought-and heat- tolerant and resistant breeds and varieties; (2) intensified research in agricultural practices; (3) increasing the efficiency of water use within agriculture; and (4) integrating all these strategic goals within a sustainable research framework. Finally, the research calls for rapid action and implementation. Significance:  • The framework is proposed for stakeholders and policymakers in higher education, agriculture and resources management in South Africa for new research horizons at national level to improve overall agricultural sustainability by 2030 as stipulated by the Millennium Development Goals

How Can Sustainable Agriculture Increase Climate Resilience? A Systematic Review

In the last few decades, a great deal has been written on the use of sustainable agriculture to improve the resilience of ecosystem services to climate change. However, no tangible and systematic evidence exists on how this agriculture would participate in alleviating impacts on vulnerable rural communities. This paper provides a narrative systematic review (SR) integrated with a bibliometric analysis and a concept network analysis to determine how, in this changing climate, sustainable agriculture can increase the resilience of agrosystems. Our search ranged from the date of the first relevant article until the end of 2018. The results generated demonstrated the following: (a) Only single practices and methods have been studied to assess the impacts on single ecosystem services; (b) Soil quality and health are considered a key indicator of sustainable agriculture; (c) Although the assessed practices and methods were shown to improve the biodiversity of agrosystems, which makes them more resilient to extreme climate events, we are still far from developing interdisciplinary and multidimensional agriculture that integrates all management aspects and generates a full range of ecosystem services. In conclusion, this study addressed the following recommendations for the scientific community and policymakers to orient future research strategies and efforts: (a) The integration of all agrosystem services into sustainable management using an ecosystem-based approach on a life-cycle basis using the Life Cycle Assessment (LCA) method; (b) Improving the scientific understanding of traditional knowledge to facilitate greater synergy and further integration; (c) The unification of assessment methods and indicators for the quantification of impacts; (d) The creation of a platform to share, monitor, screen, and approve assessments and evaluations of sustainable agriculture by region

Biological Approaches Promise Innovative and Sustainable Management of Powdery Mildew in Lebanese Squash

Biological management techniques act as a promising and sustainable alternative to alleviate pathogen-induced losses, improve ecosystem functions, and reinforce the resilience of agricultural systems. Lebanese squash production has been threatened by powdery mildew disease caused by the fungus Podosphaera xanthii. Very few studies, even unpublished ones, stress the evaluation of biological control approaches in the Lebanese agriculture sector. Here, we have aimed to evaluate the effect of five safe biological treatments (olive soap, sodium bicarbonate, garlic extract, horsetail, and compost tea) in the management of powdery mildew on Lebanese squash in organic open field conditions. Plants were treated after the first spots of powdery mildew appeared on leaves. We then examined the leaves to evaluate disease incidence and severity, and to compare the ability of the five treatments to reduce powdery mildew disease and incidence, in comparison with the untreated control. Plants treated with sodium bicarbonate and garlic extract were the least affected by powdery mildew regarding disease incidence and severity, while tea compost proved to be the least effective product. Organic management of vegetable crops is extremely important in order to ensure global food security and reduce pesticide applications

Biological Approaches Promise Innovative and Sustainable Management of Powdery Mildew in Lebanese Squash

Biological management techniques act as a promising and sustainable alternative to alleviate pathogen-induced losses, improve ecosystem functions, and reinforce the resilience of agricultural systems. Lebanese squash production has been threatened by powdery mildew disease caused by the fungus Podosphaera xanthii. Very few studies, even unpublished ones, stress the evaluation of biological control approaches in the Lebanese agriculture sector. Here, we have aimed to evaluate the effect of five safe biological treatments (olive soap, sodium bicarbonate, garlic extract, horsetail, and compost tea) in the management of powdery mildew on Lebanese squash in organic open field conditions. Plants were treated after the first spots of powdery mildew appeared on leaves. We then examined the leaves to evaluate disease incidence and severity, and to compare the ability of the five treatments to reduce powdery mildew disease and incidence, in comparison with the untreated control. Plants treated with sodium bicarbonate and garlic extract were the least affected by powdery mildew regarding disease incidence and severity, while tea compost proved to be the least effective product. Organic management of vegetable crops is extremely important in order to ensure global food security and reduce pesticide applications