THE ROLE OF HUMIC AND AMINO ACIDS IN IMPROVING GROWTH AND QUALITY OF PASPALUM TURF

A field experiment was conducted at the nursery of Hort. Res. Inst., ARC., Giza, Egypt during 2014 and 2015 seasons to study the effect of spraying with humic acid at 0, 5 and 10 ml/l and adding mixture of amino acids as soil drench at 0, 1 and 2 g/pot individually or in combinations on growth, covering rate (%) and chemical composition of seashore paspalum (Paspalum vaginatum, Swartz.) plants grown in 40-cm-diameter plastic pots filled with sand medium.The obtained results showed that all treatments caused improvement in plant height, covering rate, number of plants/pot and herb fresh and dry weights compared with control, also, chemical results of chlorophyll a, b and carotenoids content in the leaves and total sugars content in the herb. The best treatments used resulted from the combining between humic acid at 10 ml/l and mixture of amino acids at 2 g/pot which recorded the highest means.Hence, in order to get the best growth and quality of paspalum plants, it could be recommended to spray with humic acid at 10 ml/l and adding a mixture of amino acids as soil drench at 2 g/pot six times with one month interval

Aging changes of the testis in albino rat: light, electron microscopic, morphometric, immunohistochemical and biochemical study

Background: Aging has a deleterious effect on the morphology of the male reproductive system which might, in turn, lead to changes in spermatogenesis and consequently, decrease in both quality and quantity of spermatozoa. Materials and methods: The present study elucidated the histological and ultrastructural changes of testes of adult albino rats during aging and applied morphometric measures to obtain quantitative data for these changes. The oxidative and antioxidative markers of aged testes were also assessed. Results: The results documented the presence of age-related regressive structural changes of the testis accompanied with an increase in the apoptosis and a decrease in the proliferative capacity. Conclusions: The biochemical results gave evidence of an imbalance between the oxidative damage and the antioxidant defence indicating increased oxidative stress of aged testis

ZOONOTIC AND HISTO-PATHOLOGICAL ASPECTS OF VARIOUS TOXOPLASMA GONDII STRAINS IN FEMALE RATS

Background: Toxoplasma gondii is one of the most opportunistic zoonotic protozoan, typifying three virulent types; I, II, and III; with possible characteristic pathological and serological aspects, showing zoonotic and reproductive impact. The present investigation dealt with the concept, and focused on studying the susceptibility of female rats to cerebral and uterine toxoplasmosis, with reference to diversity of T. gondii virulent types through histo-pathological and serological assays. Materials and Methods: A total of thirty pathogen free Sprague-Dawley female rats were used for the study. They were divided into two main groups (acute and chronic) groups: Both were further subdivided into three additional subgroups in correspondence with the three T. gondii types; I, II and III (local isolates) strains. ELISA IgM / IgG as well as pathological investigations of visceral organs; brain and muscles were done prior to 7 days post Inoculation. (DPI) and 35 DPI, represent the acute and chronic stages respectively. Results: The results revealed that type I possess, a higher percentage for inducing cerebral and uterine toxoplasmosis in addition to higher elevation of both parasite load and immunoglobulin titers; while cystogenic types II and III showed lower values. Conclusion: It could be concluded that; successful recognition of cerebral and uterine toxoplasmosis were sequential to the three T. gondii virulent types, and reflects the possible match of pathological and serological alters with humans at similar circumstance

Lysine and Glutamic Acids as the End Products of Multi-response of Optimized Fermented Medium by Mucor mucedo KP736529

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Xylene Isomerization using Hierarchically Mesoporous ZSM-5

The current study described the synthesis of H-ZSM-5 zeolites with hierarchical micro-meso- porosity (HM-ZSM-5-x) via the soft-templating route, employing organosilane surfactant, 3-[(trimethoxysilyl) propyl]octyldimethyl-ammonium chloride, as the mesoporous template. The catalytic performance was examined in the isomerization of o-xylene in a fixed-bed reactor at atmospheric pressure. Many techniques were conducted to characterize the catalysts. The X-ray diffraction (XRD) and Fourier Transform Infra Red (FTIR) results affirmed that all mesoporous zeolites possess the characteristic MFI structure, as well as good crystallinity. The N2 physisorption measurements signified that all HM-ZSM-5-x samples have higher surface areas and pore volumes than the micro-ZSM-5 sample, with the mesopores accounting for the vast majority of the total surface areas and pore volumes of HM-ZSM-5-x samples. Moreover, the mesoporosity of the obtained HM-ZSM-5-x zeolites can be simply tuned via the variation of the amount of TPOAC used. Compared with classical micro-ZSM-5, the HM-ZSM-5-0.15 sample possessed a higher o-xylene conversion and p-xylene yield that was attributed to its remarkable textural characteristics in terms of higher surface area and prevailing mesoporous character that led to a reduced diffusion limitation. Importantly, the catalyst manifested superb operational stability within 50 h, indicating high resistance against deactivation through coke deposition. Copyright © 2023 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA   License (https://creativecommons.org/licenses/by-sa/4.0)

Ecological and Pathological Study of T. gondii Egyptian Rat Isolates Reference to Biological & Genetic Typescripts

Abstract: Toxoplasma gondii is zoonotic opportunistic protozoan of public health impact. It has usual clonal population of three virulent human and animal types: I, II, and III. The commercial rats are frequently harboring the dormant T. gondii tissue cysts in their tissues. So, rats consider the major prey for outdoor cats that regularly stimulating it to shed and contaminate the environment with oocysts infective stage which maximizes human and animals' toxoplasmosis. The aim of the present study is to identify the biological and molecular typescripts of naturally infected commercial rats along with serological, mice viability and histo-pathological assays for clarifying to how extent the indirect zoonotic bio-hazards sequence to rats harboring T. gondiivirulent types. A total number of 278 commercial rats were recognized as R. norvegicus (n=74), Rattus rattus rattus(n=108)and Rattus rattus frugivorus (n=96), were collected from different rural and urban sites from Cairo and Giza governorates. Blood and tissue samples were exposed to serological and mice viability test along with histopathological exam. Also, the tissues of inoculated mice were exposed tokitten's viability test for detecting oocyst shedding characters of rat isolates. Parasite load, LD50 and LD100 of the rat isolates were detected for bio-typing. Plus to geno-typing via SAG2 PCR amplification products by electrophoresis analysis using tissue digest from inoculated mice. The results of microscopic and histo-pathological exam of rat tissues and inoculated mice were recorded. The percentages of successes rat isolates through mice inoculation were; (14.9), (3.7), (2.1).while, the overall prevalent of biological and genetic typescripts were; type I (17.6%), type II (52.9%) and type III (29.4%) corresponding to R. norvegicus, Rattus rattus rattus and Rattusrattus frugivorus. We were concluded that the examined commercial rats often were holding T. gondii tissue cysts corresponding to the human virulent strains I, II, and III, reflecting their role in toxoplasmosis ecology through indirect human hazard via recurrently exciting oocysts shedder cats