Effects of silver nitrate and donor plant growing conditions on production of pepper (Capsicum annuum L.) haploid embryos via anther culture

The responses of pepper anthers to different concentrations of silver nitrate and in dependence of the donor plant growing conditions were studied for haploid embryo production via anther culture. U-247 and U-238 genotypes, which were selected from a local paprika pepper population, were used as plant materials. Flower buds were collected from the plants grown either in greenhouse or in open field conditions. Four different silver nitrate concentrations (5, 10, 15 and 20 mg l-1) were tested. Haploid embryos were obtained from all the concentrations tested, but with different production rates. The highest embryo formation (45.7 embryos per 100 anthers) was obtained from the genotype U-247 in the medium containing 15 mg l-1 silver nitrate. The anthers taken from the plants grown in the greenhouse produced more embryos than that of the open field conditions

Melon breeding for resistance to Fusarium wilt using molecular markers and dihaploidization

In this breeding program aimed at developing resistant varieties to Fusarium wilt in Turkish melon type, molecular markers and dihaploidization techniques were used. Cum334 was used as the donor parent in a backcross program and the haploid plants were obtained via pollination with the irradiated pollen from the BC3 population to shorten the breeding period. Four different embryo rescue methods were tested to extract haploid embryos. According to the results, the best methods were found to be "inspecting seeds on the light" and "sowing seeds directly to nutrient media", and a total of the 122 haploid plants were obtained. The haploid plants were screened by molecular markers for fom1 and fom2 genes. A Simple Sequence Repeat (SSR) and a Randomly Amplified Polymorphic DNA (RAPD) marker were used for fom2 gene and a Cleaved Amplified Polymorphic Sequences (CAPS) and three Sequence Characterized Amplified Region (SCAR) markers were used for fom1 gene. While SSR (SSR180) did not segregate the haploid plants the RAPD marker (OPG17) segregated those haploid plants for fom2 gene. Haploid plants were screened for fom1 by a SCAR marker (SB17). Among the tested haploids, 41% of plants were found to be resistant and the rest of 59% were susceptible to the Fusarium wilt races 0 and 2. On the other hand while 34% of plants were resistant 66% were susceptible to the races 0 and 1 of the Fusarium oxysporum f.sp. melonis

Carotid intima-media thickness in chronic obstructive pulmonary disease and survival: A multicenter prospective study

Introduction: Chronic obstructive pulmonary disease (COPD) is associated with increased cardiovascular morbidity and mortality. Carotid intima-media thickness (CIMT) is a noninvasive method assessing atherosclerosis. Objective: It was aimed to determine relationship and survival between COPD and CIMT. Methods: CIMT was measured using Doppler ultrasound (USG) in 668 stable COPD patients at 24 centers. Patients were followed-up for 2 years. Results: There were 610 patients who completed the study. There were 200 patients CIMT with <0.78 mm (group 1), and 410 with CIMT ? 0.78 mm (group 2). There was a significant difference at the parameters of age, gender, smoking load, biomass exposure, GOLD groups and degree of airway obstruction (FEV1) between groups 1 and 2. Our results revealed positive correlations between mean CIMT and age, smoking load (pack-years), biomass exposure (years), exacerbation rate (last year), duration of hypertension (years) and cholesterol level; negative correlations between CIMT and FEV1 (P < 0.05). According to logistic regression model, compared with group A, risk of CIMT increase was 2.2-fold in group B, 9.7-fold in group C and 4.4-fold in group D (P < 0.05). Risk of CIMT increase was also related with cholesterol level (P < 0.05). Compared with infrequent exacerbation, it was 2.8-fold in the patients with frequent exacerbation (P < 0.05). The mean survival time was slightly higher in group 1, but not significant (23.9 vs 21.8 months) (P > 0.05). Conclusion: This study is the first regarding CIMT with combined GOLD assessment groups. It has revealed important findings supporting the increase in atherosclerosis risk in COPD patients. We recommend Doppler USG of the carotid artery in COPD patients at severe stages. © 2019 John Wiley & Sons Lt

Decreased erythrocyte deformability and increased erythrocyte aggregation in patients with pulmonary hypertension

Abstract Not Availabl

Prognostic Role Of The European Society Of Cardiology Model And Simplified Pulmonary Embolism Severity Index In Pulmonary Embolism: Results From The Turkey Pulmonary Embolism Group (tupeg) Study

WOS: 00020983820100

spontaneously hypertensive rats

This study aimed to investigate the effects of moderate intensity swimming exercise (10 weeks) followed by detraining (for five and 10 weeks) on oxidative stress levels of heart, lung, kidney, and liver tissues and systolic blood pressure (SBP) of spontaneously hypertensive rats (SHR). SHR and control rats were randomized into sedentary, exercised, detrained (5 weeks) and late-detrained (10 weeks) groups. Corresponding sedentary rats were grouped as time 1-2-3. Exercise of 60 min, 5 days/week/10 weeks was applied. Detraining rats underwent the same training protocol and then discontinued training during next 5, 10 weeks. SBP was measured by tail-cuff method. Tissue total oxidant/antioxidant status was measured using a commercial kit and oxidative stress index (OSI) was calculated. Exercise training slightly decreased tissue OSI of SHR and reduced SBP of both groups. Tissue OSI of SHR were higher than WKY and aging resulted in increment of oxidants in groups. detraining yielded time-dependent increments in oxidative stress of all tissues and SBP of both rat groups. Although short-term cessations may be tolerated, our results emphasize the importance of exercising as a way of life for cardiovascular well-being in hypertensives or in individuals who are genetically under risk of hypertension

Detraining reverses exercise-induced improvement in blood pressure associated with decrements of oxidative stress in various tissues in spontaneously hypertensive rats

This study aimed to investigate the effects of moderate intensity swimming exercise (10 weeks) followed by detraining (for five and 10 weeks) on oxidative stress levels of heart, lung, kidney, and liver tissues and systolic blood pressure (SBP) of spontaneously hypertensive rats (SHR). SHR and control rats were randomized into sedentary, exercised, detrained (5 weeks) and late-detrained (10 weeks) groups. Corresponding sedentary rats were grouped as time 1-2-3. Exercise of 60 min, 5 days/week/10 weeks was applied. Detraining rats underwent the same training protocol and then discontinued training during next 5, 10 weeks. SBP was measured by tail-cuff method. Tissue total oxidant/antioxidant status was measured using a commercial kit and oxidative stress index (OSI) was calculated. Exercise training slightly decreased tissue OSI of SHR and reduced SBP of both groups. Tissue OSI of SHR were higher than WKY and aging resulted in increment of oxidants in groups. detraining yielded time-dependent increments in oxidative stress of all tissues and SBP of both rat groups. Although short-term cessations may be tolerated, our results emphasize the importance of exercising as a way of life for cardiovascular well-being in hypertensives or in individuals who are genetically under risk of hypertension. © 2015, Springer Science+Business Media New York