Pedigree analysis of the Spanish grapevine cultivar 'Hebén'

The use of Single Nucleotide Polymorphism (SNP) markers allows genetic identification and parentage analysis of grapevine cultivars. Ancient cultivars like 'Hebén' were widely cultivated in the Iberian Peninsula along its history and likely contributed to the origin of varieties which are still cultivated today. The goal of our study was to search for possible first-degree relationships between the female variety 'Hebén' and other Iberian and Mediterranean cultivars. For this purpose, we used a set of 261 SNP markers which allowed identifying 23 trios and 37 parent-offspring relationships supported by high overall likelihood ratios (LOD scores). The results shed light on the relationships among several cultivars from Spain and Portugal, and demonstrated that 'Hebén' is a major founder of current varieties in the Iberian Peninsula viticulture, and contributed to spread the chlorotype A in this region

Pedigree analysis of the Spanish grapevine cultivar 'Hebén'

The use of Single Nucleotide Polymorphism (SNP) markers allows genetic identification and parentage analysis of grapevine cultivars. Ancient cultivars like 'Hebén' were widely cultivated in the Iberian Peninsula along its history and likely contributed to the origin of varieties which are still cultivated today. The goal of our study was to search for possible first-degree relationships between the female variety 'Hebén' and other Iberian and Mediterranean cultivars. For this purpose, we used a set of 261 SNP markers which allowed identifying 23 trios and 37 parent-offspring relationships supported by high overall likelihood ratios (LOD scores). The results shed light on the relationships among several cultivars from Spain and Portugal, and demonstrated that 'Hebén' is a major founder of current varieties in the Iberian Peninsula viticulture, and contributed to spread the chlorotype A in this region.L. H. ZINELABIDINE was supported by a fellowship from the Agencia Española de Cooperación Internacional. J. CUNHA was supported by a Post-doctoral fellowship “Fundação para a Ciência e Tecnologia” (SFRH/BPD/ 74895/2010). LHZ and JC were supported by COST action FA1003 “East-West Collaboration for Grapevine Diversity Exploration and Mobilization of Adaptive Traits for Breeding”, from European Cooperation in the Field of Scientific and Technical Research, through the funding of short scientific missions at the ICVV.Peer Reviewe

Pedigree analysis of the Spanish grapevine cultivar Heben

Trabajo presentado en la Final Conference Progress in Vitis Vinifera diversity evaluation and use, celebrada en Lisboa del 7 al 9 de octubre de 2014.Cost action FA1003 - GRAPENET. East-West Collaboration for Grapevine Diversity Exploration and Mobilization of Adaptive Traits for Breeding.Several studies have shown that some varieties have had a large contribution in the production of varieties in certain regions, like ‘Pinot’ and ‘Heunisch Weiss’ in France. Previous results had pointed out that the old Spanish female variety ‘Hebén’, known as ‘Mourisco Branco’ in Portugal, could be one of those varieties with a remarkable role in the Iberian Peninsula. This ancient winegrape cultivar was already described in Spain in the 16th century as a white variety of grapevine producing large and sparse bunches of grape with big seeds.Peer Reviewe

Grapevine cultivar 'Alfrocheiro' or 'Bruñal' plays a primary role in the relationship among Iberian grapevines

The grapevine cultivar known in Portugal as 'Alfrocheiro', and in Spain as 'Bastardo Negro', 'Bruñal' or 'Baboso Negro', plays a central role in the genetic network of the Iberian Peninsula grapevine cultivars. Three sets of different molecular markers, SNPs, nSSRs and cpSSRs, revealed more than twenty parent-offspring links with this cultivar. 'Alfrocheiro' chlorotype is definitely Western European but their parents are still unknown. The distribution of the cultivar, their offspring as well as the two main co-parents direct to a geographic origin around the Portuguese and Spanish border. This cultivar and their progenies represent about 15 % of the total grapevine acreage in Portugal, stressing the importance of 'Alfrocheiro' in the Portuguese wine character

Grapevine cultivar "Alfrocheiro" or "Bruñal" plays a primary role in the relationship among iberian grapevines

The grapevine cultivar known in Portugal as 'Alfrocheiro', and in Spain as 'Bastardo Negro', 'Bruñal' or 'Baboso Negro', plays a central role in the genetic network of the Iberian Peninsula grapevine cultivars. Three sets of different molecular markers, SNPs, nSSRs and cpSSRs, revealed more than twenty parent-offspring links with this cultivar. 'Alfrocheiro' chlorotype is definitely Western European but their parents are still unknown. The distribution of the cultivar, their offspring as well as the two main co-parents direct to a geographic origin around the Portuguese and Spanish border. This cultivar and their progenies represent about 15 % of the total grapevine acreage in Portugal, stressing the importance of 'Alfrocheiro' in the Portuguese wine character.This research was supported by: IVV – Instituto da Vinha e do Vinho (Ministério da Agricultura e do Mar-MAM); Programa de Desenvolvimento Rural (PRODER - Ação 2.2.3.1. - PA 18621 - MAM) and J. CUNHA was supported by a Post-doctoral fellowship >Fundação para a Ciência e Tecnologia> (SFRH/BPD/74895/2010) and a short scientific mission in the ICVV was part of COST action, FA 1003 from European Cooperation in the Field of Scientific and Technical Research. L. H. ZINELABIDINE was supported by a fellowship from the Agencia Española de Cooperación Internacional and a short-term scientific mission from COST FA1003 >East-West Collaboration for Grapevine Diversity Exploration and Mobilization of Adaptive Traits for Breeding>.Peer Reviewe

Grapevine cultivar “Alfrocheiro” or “Bastardo Negro" plays a primary role in Iberian grapevine diversity

Trabajo presentado en la Final Conference Progress in Vitis Vinifera diversity evaluation and use, celebrada en Lisboa del 7 al 9 de octubre de 2014.Cost action FA1003 - GRAPENET. East-West Collaboration for Grapevine Diversity Exploration and Mobilization of Adaptive Traits for Breeding.The grapevine cultivar known in Portugal as ‘Alfrocheiro’, and in Spain as ’Bastardo Negro’, ’Bruñal’ or ’Baboso Negro’, plays a central role in the genetic network of the Iberian Peninsula grapevine cultivars. ‘Alfrocheiro’ was already identified as a progenitor of several cultivars either by SSRs or by SNPs but until now it progenitors are unknown.Peer Reviewe

Genetic diversity of the grapevine (Vitis vinifera L.) cultivars most utilized for wine production in Portugal

Portugal is very rich in grapevine biodiversity but nowadays only a small number of cultivars (13 black and 3 white) with defined enological characteristics are being used. However, there are still 51 cultivars with significant expression in the country which have a great potential to be used in the establishment of new vineyards. Considering the importance of identifying and understanding the genetic relatedness of the main cultivars used for wine making in Portugal, those cultivars were genotyped with the 6 SSRs loci VVMD5, VVMD7, VVMD27, VrZag62, VrZag79 and VVS2 that are recommended by the Office de la Vigne et du Vin for the elaboration of the worldwide database containing the alleles of all the known grapevine cultivars. A total of 49 alleles were detected with an average allele number of 8.17 per locus. The SSR profile of the 51 cultivars, at the 6 evaluated loci, indicates that all can be distinguished from each other. In addition, this study produces information on the relationship of these 51 grapevine cultivars with cultivars of other important Mediterranean wine producing regions

Guiding principles for identification, evaluation and conservation of Vitis vinifera L. subsp. sylvestris

Conservation of grapevine genetic resources is an important and long lasting task. Here, partners of the InWiGrape Activity of the European Cooperative Programme for Plant Genetic Resources have proposed a set of descriptors that will assist in identification, conservation and study of genetic resources of Vitis vinifera L. subsp. sylvestris. A distribution map of Vitis vinifera L. subsp. sylvestris populations in Europe was produced, with on-line access through the European Vitis Database. The several different aspects of conservation of Vitis vinifera L. subsp. sylvestris including bibliographical references, identification in the wild, in situ and ex situ conservation have been discussed. The descriptors and the map will assist different stakeholders, working on biodiversity and ecosystems in more effective conservation of wild grapevine genetic resources

Description of the Vitis vinifera L. phenotypic variability in eno-carpological traits by a Euro-Asiatic collaborative network among ampelographic collections

The grapevine intra-specific variability captured an increasing interest during the last decades, as demonstrated by the number of recently funded European projects focused on the grapevine biodiversity preservation. However, nowadays, crop plants are mainly characterized by genotyping methods. The present work summarizes the phenotype data collected among 20 ampelographic collections spread in 15 countries, covering mostly of the viticultural areas in the Euro-Asiatic range: from Portugal to Armenia and from Cyprus to Luxembourg. Together with agro-climatic characterization of the experimental site, in two years, about 2400 accessions were described, following a common experimental protocol mainly focused on the carpological and oenological traits, obtaining a general overview of the distribution of the considered phenotypic traits in the cultivated Vitis vinifera species. The most replicated cultivars were selected and, for the subset of these reference cultivars, their behavior in the different environmental conditions over sites and years was described by ANOVA methods