Mass Production of Mesenchymal Stem Cells — Impact of Bioreactor Design and Flow Conditions on Proliferation and Differentiation

The book serves as a good starting point for anyone interested in the application of tissue engineering. It offers a colorful mix of topics, which explain the obstacles and possible solutions for TE applications. The first part covers the use of adult stem cells and their applications. The following chapters offer an insight into the development of a tailored biomaterial for organ replacement and highlight the importance of cell-biomaterial interaction. In summary, this book offers insights into a wide variety of cells, biomaterials, interfaces and applications of the next generation biotechnology, which is tissue engineering

Recommendations for the engineering characterization of single-use bioreactors

Single-use bioreactors have been available for more than 15 years and are nowadays widely accepted for a broad range of applications. However, process engineering data for these bioreactors, including volumetric mass transfer coefficients, mixing times and power inputs, are still limited. Furthermore, these data are often generated using a number of different methods, making comparisons difficult. In addition, the large variety of bioreactor types and their mixing principles (stirred, wave-mixed, orbitally-shaken, etc.) increases the difficulty in comparing the engineering data. In order to facilitate the usage of single-use bioreactors, the DECHEMA Upstream Processing (USP) expert group on ‘Single-use technology in biopharmaceutical manufacturing’ has developed recommendations for the process engineering characterization of single-use bioreactors. The methods are based on procedures that were previously developed for multi-use bioreactors and were tested in universities as well as companies (both from the supplier and user sides) for their robustness. The validated recommendations now include measurements of volumetric mass transfer coefficient, mixing time and power input. These parameters are experimentally determined using the dynamic gassing-out method, the decolorization method or sensor method, and the torque method respectively. The poster gives an overview of the fundamentals and procedures of the methods applied, and current results from the interlaboratory tests. Recent foci include the measurement of carbon dioxide gas-liquid mass transfer and the determination of mechanical stress due to hydrodynamics

The effect of relationship status on communicating emotions through touch

Research into emotional communication to date has largely focused on facial and vocal expressions. In contrast, recent studies by Hertenstein, Keltner, App, Bulleit, and Jaskolka (2006) and Hertenstein, Holmes, McCullough, and Keltner (2009) exploring nonverbal communication of emotion discovered that people could identify anger, disgust, fear, gratitude, happiness, love, sadness and sympathy from the experience of being touched on either the arm or body by a stranger, without seeing the touch. The study showed that strangers were unable to communicate the self-focused emotions embarrassment, envy and pride, or the universal emotion surprise. Literature relating to touch indicates that the interpretation of a tactile experience is significantly influenced by the relationship between the touchers (Coan, Schaefer, & Davidson, 2006). The present study compared the ability of romantic couples and strangers to communicate emotions solely via touch. Results showed that both strangers and romantic couples were able to communicate universal and prosocial emotions, whereas only romantic couples were able to communicate the self-focused emotions envy and pride

Understanding the local structure of Eu3+- and Y3+-stabilized zirconia: insights from luminescence and X-ray absorption spectroscopic investigations

This study combines bulk structural and spectroscopic investigations of Eu$^{3+}$- or Y$^{3+}$/Eu$^{3+}$ co-doped tetragonal and cubic zirconia polymorphs to gain an indepth understanding of the solid solution formation process. Our bulk structural characterizations show that the dopant is homogenously distributed in the ZrO$_{2}$ host structure resulting in an increase of the bulk symmetry with increasing dopant substitution (from 8 to 26 mol%). The local site symmetry around the Eu$^{3+}$ dopant, however, determined with luminescence spectroscopy (TRLFS), remains low in all samples. Results obtained with X-ray pair distribution function and X-ray absorption spectroscopy show that the average coordination environment in the stabilized zirconia structures remains practically unchanged. Despite this very constant average dopant environment, siteselective TRLFS data show the presence of three nonequivalent Eu$^{3+}$ environments in the ZrO$_{2}$ solid structures. These Eu$^{3+}$ environments are assumed to arise from Eu$^{3+}$ incorporation at superficial sites, which increase in abundance as the size of the crystallites decrease, and incorporation on two bulk sites differing in the location of the oxygen vacancies with respect to the dopant cation