STUDYING OF POLLEN MORPHOLOGY SOME AGROPYRON GARTNER, AEGILOPS L., TRITICUM L., SECALE L. AND HORDEUM L. SPECIES

Bu çalısmada ekonomik açıdan önemli olan Agropyron Gaertner, Aegilops L., Triticum L., Secale L., ve Hordeum L. cinslerine ait 14 takson polen morfolojik özelliklerine göre incelenmistir. Bu cinsler Triticeae Dumort (Gramineae) tribüsü içerisinde bulunmaktadır. IM ve SEM çalısmaları incelenen taksonların monoporat, operkulat, annulat ve ekzin ornamentasyonunun ise skabrat veya insular oldugunu göstermistir.In this research study, 14 taxa belonging to economically important genera Agropyron Gaertner, Aegilops L., Triticum L., Secale L., and Hordeum L. have been investigated regarding their polen morphological features. These genera are included in tribe Triticeae Dumort (Grramineae). LM and SEM studies showed that studied taxa are monoporate, operculate, annulate and also exine ornamentation of taxa scabrate or insular

Investigation of Carbapenemase Genes and Molecular Epidemiology of Enterobacteriaceae Strains Isolated between 2010-2014 in a University Hospital

WOS: 000428835600001PubMed ID: 29642825The worldwide spread of carbapenemase producing Enterobacteriaceae isolates has become a major threat of public health. This worrisome situation leads the development of new methods for carbapenemase screening, detection, prevention of spread and epidemiological data collection as mandatory. In this study, it was aimed to investigate existence and distribution of carbapenemase-encoding genes (CEGs) among carbapenem-resistant Enterobacteriaceae isolated from various clinical samples in Ankara University Faculty of Medicine, Ibni Sina Hospital, Central Microbiology Laboratory between June 2010-May 2014 and detect their clonal relationship. A total of 112 non-repetitive Enterobacteriaceae isolates which were intermediate or resistant to ertapenem were identified by using Phoenix (BD Diagnostic Systems, Sparks, USA) automated microbiology system. After DNA extraction from the isolates, 11 carbapenemase-encoding genes (CEGs) (bla(IMP), bla(VIM), bla(SPM), bla(KPC), bla(NDM), bla(OXA-483), bla(GIM), bla(SIM), bLa(AIM), blaDIM ye bla(BIC)) were detected with PCR. The clonal relationship among the isolates was determined by PFGE method following digestion with Xbal DNA macrorestriction endonuclease. Among 112 isolates Klebsiella pneumoniae was the most frequent (n=79, 70.5%) bacteria followed by Escherichia coli (n=15, 13.4%), Enterobacter cloacae (n=10, 8.9%), Enterobacter aerogenes (n=4, 3.6%) and Klebsiella oxytoca (n=4, 3.6%) respectively. bla(OXA-48) was the most frequent gene detected. Among 83 (74.1%) isolates bla(OXA-48) was detected alone and in 7 (6.3%) of the isolates it was identified with bla(VIM) gene coexistence. blavim gene was identified as the second most frequent CEG among the isolates. bla(VIM) gene was detected positive in 9 (8%) isolates. bla(NDM) gene was identified in 2 (1.8%) isolates. Ten of the K.pneumoniae isolates with identical PFGE pattern were named as pulsotype B. These isolates were found to be similar in terms of isolate location, isolation dates, antibiotic resistance patterns and the carbapenemase genes they carry, and are considered to be potential outbreak isolates originated from intensive care units. On the other hand CEGs were found in the clinical samples obtained from five out-patients suggesting that community-acquired infections may also arise due to carbapenemase producing Enterobacteriaceae in our country where bla(OXA-48) producers are endemic. According to this study, bla(OXA-48) producing gram negative bacteria were frequent in our hospital. The prevalance of bla(VIM) gene among metallo-beta-lactamases and coexistence with bla(OXA-48) gene was remarkable. The frequency of bla(NDM) producing isolates in our hospital was not detected as high yet. In this study, the identification of carbapenemase producing bacteria as outbreak strains in our hospital indicated that cross-sectional surveillance for carbapenemase-producing bacteria from each patient was valuable in terms of early diagnosis of outbreaks

POLLEN MORPHOLOGY OF AGROPYRON GAERTNER IN TURKEY

Pollen morphology of Agropyron cristatum (L.) Gaertner. s.s. (subsp. incanum (Nabelek) Melderis and subsp. pectinatum (M. Bieb.) Tzvelev, latter including var. pectinatum and var. imbricatum (Roemer & Schultes) G. Beck) in Turkey has been studied by using light microscope and scanning electron microscope. The above-mentioned taxa are homogenous in both aperture type and exine ornamentation. Pollen grains are monoporate (rarely diporate in the case of var. imbricatum) having scabrate grouped exine surface. The scabra density and the height of scabrae as well as other morphological parameters such as annulus and operculum diameter are peculiar features for differentiation of taxa, Two different phenograms were created with the UPGMA (Unweighted Pair Group Method with Arithmetic mean) clustering technicque using quantitative measurements of the pollen grains

Pollen morphology of Agropyron Gaertner in Turkey

Pollen morphology of Agropyron cristatum (L.) Gaertner. s.s. (subsp. incanum (Náb?lek) Melderis and subsp. pectinatum (M. Bieb.) Tzvelev, latter including var. pectinatum and var. imbricatum (Roemer & Schultes) G. Beck) in Turkey has been studied by using light microscope and scanning electron microscope. The above-mentioned taxa are homogenous in both aperture type and exine ornamentation. Pollen grains are monoporate (rarely diporate in the case of var. imbricatum) having scabrate grouped exine surface. The scabra density and the height of scabrae as well as other morphological parameters such as annulus and operculum diameter are peculiar features for differentiation of taxa. Two different phenograms were created with the UPGMA (Unweighted Pair Group Method with Arithmetic mean) clustering technique using quantitative measurements of the pollen grains

Correlation of Treponemal Chemiluminescent Microparticle Immunoassay Screening Test Signal Strength Values With Reactivity of Confirmatory Testing

Background Automated chemiluminescent microparticle immunoassays (CMIAs) are the most common first step at high-volume laboratories for syphilis screening. If the initial screening test is reactive, 1 more treponemal test is required, resulting in increased cost. In this multicenter study, we aimed to determine the correlation between the CMIA signal-to-cutoff ratio (S/Co) and the confirmatory tests to reduce unnecessary confirmatory testing. Methods Eight hospitals from 5 provinces participated in this study. All laboratories used Architect Syphilis TP CMIA (Abbott Diagnostics, Abbott Park, IL) for initial screening. Treponema pallidum hemagglutination (TPHA), rapid plasma reagin (RPR), and fluorescent treponemal antibody absorption (FTA-ABS) were used as confirmatory tests according to the reverse or European Centre for Disease Prevention and Control algorithms. A receiver operating characteristic analysis was used to determine the optimal S/Co ratio to predict the confirmation results. Results We evaluated 129,346 serum samples screened by CMIA between January 2018 and December 2020. A total of 2468 samples were reactive; 2247 (91%) of them were confirmed to be positive and 221 (9%) were negative. Of the 2468 reactive specimens, 1747 (70.8%) had an S/Co ratio >= 10.4. When the S/Co ratios were >= 7.2 and >= 10.4, the specificity values were determined to be 95% and 100%, respectively. In a subgroup of 75 CMIA-positive patients, FTA-ABS was performed and 62 were positive. Among these FTA-ABS-positive patients, 24 had an S/Co ratio = 10.4, obviating the need for secondary treponemal testing in about 71% of the screening-reactive samples. This would substantially reduce the confirmatory testing volume and laboratory expenses. However, in high-risk group patients with CMIA positive results, S/Co ratio <10.4, and negative TPHA and RPR, FTA-ABS may be used for confirmation