2,682 research outputs found
York\u27s Twin Pine Camps
A brochure highlighting scenery and recreation opportunities of the Katahdin mountain range between Greenville and Millinocket, Maine. Includes several photographs and a map of the Katahdin District showing the location of York\u27s Twin Pine Camps, circa 1900
Designing for Metaphor in a Study of Adult Educators: An Exploration and Critique of Metaphor Analysis
The authors argue that designing for metaphors in case study can offer insight into how adult educators view their roles and responsibilities in the classroom. They also critique metaphor analysis, arguing that, like all dialogue with participants, metaphors must also be analyzed within the space of the interview
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In vitro expanded human CD4+CD25+ regulatory T cells suppress effector T cell proliferation.
Regulatory T cells (Tregs) have been shown to be critical in the balance between autoimmunity and tolerance and have been implicated in several human autoimmune diseases. However, the small number of Tregs in peripheral blood limits their therapeutic potential. Therefore, we developed a protocol that would allow for the expansion of Tregs while retaining their suppressive activity. We isolated CD4+CD25 hi cells from human peripheral blood and expanded them in vitro in the presence of anti-CD3 and anti-CD28 magnetic Xcyte Dynabeads and high concentrations of exogenous Interleukin (IL)-2. Tregs were effectively expanded up to 200-fold while maintaining surface expression of CD25 and other markers of Tregs: CD62L, HLA-DR, CCR6, and FOXP3. The expanded Tregs suppressed proliferation and cytokine secretion of responder PBMCs in co-cultures stimulated with anti-CD3 or alloantigen. Treg expansion is a critical first step before consideration of Tregs as a therapeutic intervention in patients with autoimmune or graft-versus-host disease
Wet Analysis of Gold-Silver Alloys of High Gold Content
Author Institution: Department of Chemistry, The Ohio State University, Columbus, OhioGold-silver alloys dissolve completely in mixtures of hydrochloric and nitric acids acids containing potassium chloride in high concentration. In an analytical procedure based on this observation, silver is separated and determined as the chloride, and gold as the metal after reduction with sulfite. Analyses of test alloys, synthetic mixtures, and ancient objects showed that this new procedure is practicable and that results of satisfactory accuracy can be obtained
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