Searching for galaxy clusters using the aperture mass statistics in 50 VLT fields

Application of the aperture mass (Map-) statistics provides a weak lensing method for the detection of cluster-sized dark matter halos. We present a new aperture filter function and maximise the effectiveness of the Map-statistics to detect cluster-sized halos using analytical models. We then use weak lensing mock catalogues generated from ray-tracing through N-body simulations, to analyse the effect of image treatment on the expected number density of halos. Using the Map-statistics, the aperture radius is typically several arcminutes, hence the aperture often lies partly outside a data field, consequently the signal-to-noise ratio of a halo detection decreases. We study these border effects analytically and by using mock catalogues. We find that the expected number density of halos decreases by a factor of two if the size of a field is comparable to the diameter of the aperture used. We finally report on the results of a weak lensing cluster search applying the Map-statistics to 50 randomly selected fields which were observed with FORS1 at the VLT. Altogether the 50 VLT fields cover an area of 0.64 square degrees. The I-band images were taken under excellent seeing conditions (average seeing 0.6 arcsec.) which results in a high number density of galaxies used for the weak lensing analysis (26/sq.arcmin). In five of the VLT fields, we detect a significant Map-signal which coincides with an overdensity of the light distribution. These detections are thus excellent candidates for shear-selected clusters.Comment: 23 pages, 5 tables, 24 figures, published in A&A, Sect. 3.5 and 7 are changed or altered; Fig. 11 is change

Cosmic shear surveys

Gravitational weak shear produced by large-scale structures of the universe induces a correlated ellipticity distribution of distant galaxies. The amplitude and evolution with angular scale of the signal depend on cosmological models and can be inverted in order to constrain the power spectrum and the cosmological parameters. We present our recent analysis of 50 uncorrelated VLT fields and the very first constrains on ($\Omega_m,\sigma_8$) and the nature of primordial fluctuations based on the join analysis of present-day cosmic shear surveys.Comment: Latex, 7 pages. To appear in the ESO Proceedings ``Deep Fields'', Garching Oct 9-12, 200

Bone loss in KLHL3 knock-in mice characterized by a pseudohypoaldosteronism type II-like phenotype is mediated by renal PTH resistance

This is the final versionPoster presented at the 43rd Annual European Calcified Tissue Society Congress, Rome, Italy, 14 - 17 May 2016Pseudohypoaldosteronism type II (PHAII) is a hereditary disease characterized by hypertension, hypercalciuria and osteopenia. PHAII is caused by mutations in with-no-lysine kinase 1 (WNK1), WNK4, or the cullin RING ligase family members kelch-like 3 (KLHL3) or cullin 3 (CUL3). All mutations result in up-regulation of the WNK signalling pathway which activates thiazide-sensitive Na-Cl cotransporters (NCC) in renal distal tubules, leading to salt retention and hypertension in PHAII. The mechanism underlying hypercalciuria in PHAII is unknown. To better understand the mechanisms leading to osteopenia in PHAII, we used KLHL3R528H/+ knock-in mice carrying the same mutation as some PHAII patients. As expected, KLHL3R528H/+ mutants exhibited hyperkalemia, hypernatremia, renal calcium wasting and increased phosphorylation of NCC in the kidney. Furthermore, KLHL3R528H/+ mutants showed elevated serum parathyroid hormone (PTH), increased bone resorption as demonstrated by elevated urinary collagen crosslinks excretion and increased osteoclast numbers in femoral cancellous bone, and reduced distal femoral cancellous bone BMD and volume as evidenced by pQCT and μCT analysis. Analysis of the expression of proteins involved in renal calcium transport revealed elevated membrane abundance of the fully glycosylated epithelial calcium channel TRPV5, decreased TRPV6 abundance, and unchanged calbindin D28k expression in KLHL3R528H/+ mutants. In contrast to the upregulated TRPV5 protein expression, TRPV5 phosphorylation was reduced in KLHL3R528H/+ mutants, suggesting downregulated TRPV5 activity. In line with a crosstalk between NCC activity and PTH-mediated TRPV5 activation, we found by 2-photon microscopy that the PTH-mediated increase in Ca2+ uptake in mouse distal tubular mpkDCT4 cells was enhanced by the NCC blocker chlorothiazide or by knockout of NCC. Taken together, our study provides a mechanistic explanation for the hypercalciuria and bone loss found in PHAII patients: elevated NCC activity in KLHL3R528H/+ mice blocks PTH-mediated TRPV5 activation, leading to renal PTH resistance with subsequent renal Ca wasting and a counter-regulatory PTH-induced bone loss

ESO Imaging Survey VII. Distant Cluster Candidates over 12 square degrees

In this paper the list of candidate clusters identified from the I-band data of the ESO Imaging Survey (EIS) is completed using the images obtained over a total area of about 12 square degrees. Together with the data reported earlier the total I-band coverage of EIS is 17 square degrees, which has yielded a sample of 252 cluster candidates in the redshift range 0.2 \lsim z \lsim 1.3. This is the largest optically-selected sample currently available in the Southern Hemisphere. It is also well distributed in the sky thus providing targets for a variety of VLT programs nearly year round.Comment: 5 pages, 3 figures, submitted to Astronomy & Astrophysic

GaBoDS: The Garching-Bonn Deep Survey -- II. Confirmation of EIS cluster candidates by weak gravitational lensing

We report the first confirmation of colour-selected galaxy cluster candidates by means of weak gravitational lensing. Significant lensing signals were identified in the course of the shear-selection programme of dark matter haloes in the Garching-Bonn Deep Survey, which currently covers 20 square degrees of deep, high-quality imaging data on the southern sky. The detection was made in a field that was previously covered by the ESO Imaging Survey (EIS) in 1997. A highly significant shear-selected mass-concentration perfectly coincides with the richest EIS cluster candidate at z~0.2, thus confirming its cluster nature. Several other shear patterns in the field can also be identified with cluster candidates, one of which could possibly be part of a filament at z~0.45.Comment: 4 pages, 4 figures, submitted to A&A Letter

Histomorphometric Analysis of the Rat Proximal Tibial Metaphysis by “Linear Scanning”

Twenty-four female Sprague-Dawley rats (10 weeks old, 200g BW) were either sham-operated (n = 6) or ovariectomized (ovx). Ovx rats were divided into 3 groups (n = 6 each) : ovx; ovx + 1,25(OH) 2D3; ovx + l,25(OH)2D3 + 1,24,25(OH)3D3. The vitamin D metabolites were fed orally starting the day after surgery. After 7 weeks all rats were sacrificed and the proximal tibiae were processed undecalcified for quantitative histomorphometry. Conventional histomorphometric analysis of the distal zone (\u3e 1 mm from the growth cartilage) of the tibial metaphysis revealed a dramatic loss of cancellous bone mass in ovx rats. Both l,25(OH)2D3 and the combination of l,25(OH)2D3 with l,24,25(OH)3D3 prevented the bone loss in the distal zone in ovx animals. Measurements in the proximal zone (\u3c 1 mm from the growth cartilage) of the tibial metaphysis were performed with a newly developed technique that utilizes the advantages of automatic image analysis, and that we propose to name linear scanning . This method revealed a significantly decreased hard tissue mass at about 100 μm and within 800 to 950 μm distance from the growth plate in ovx rats. However, ovx rats reached normal amounts of hard tissue within 250 to 450 μm from the growth plate. The results obtained by linear scanning suggest that the obvious loss of cancellous bone mass in the distal zone of the tibial metaphysis in growing ovx rats is not a consequence of structural changes in the proximal zone