10 research outputs found
The mediterranean dietary pattern and breast cancer risk in Greek-Cypriot women: a case-control study
Background: Diet has long been suspected to impact on breast cancer risk. In this study we evaluated whether
the degree of adherence to a Mediterranean diet pattern modifies breast cancer risk amongst Greek-Cypriot
women.
Methods: Subjects included 935 cases and 817 controls, all participating in the MASTOS case-control study in
Cyprus. The study was approved by the Cyprus National Bioethics Committee. Information on dietary intakes was
collected using an interviewer administered 32-item Food Frequency Questionnaire. Information on demographic,
anthropometric, lifestyle, and other confounding factors was also collected. Adherence to the Mediterranean Diet
pattern was assessed using two a-priory defined diet scores. In addition, dietary patterns specific to our population
were derived using Principal Component Analysis (PCA). Logistic regression models were used to assess the
association between the dietary patters and breast cancer risk.
Results: There was no association with breast cancer risk for either score, however, higher consumptions of
vegetables, fish and olive oil, were independently associated with decreased risk. In addition, the PCA derived
component which included vegetables, fruit, fish and legumes was shown to significantly reduce risk of breast
cancer (ORs across quartiles of increasing levels of consumption: 0.89 95%CI: 0.65-1.22, 0.64 95%CI: 0.47-0.88, 0.67 95%CI: 0.49-0.92, P trend < 0.0001), even after adjustment for relevant confounders.
Conclusions: Our results suggest that adherence to a diet pattern rich in vegetables, fish, legumes and olive oil
may favorably influence the risk of breast cancer. This study is the first investigation of dietary effects on breast
cancer risk in Cyprus, a country whose population has traditionally adhered to the Mediterranean diet
Influenza Virus Ribonucleoprotein Complexes Gain Preferential Access to Cellular Export Machinery through Chromatin Targeting
In contrast to most RNA viruses, influenza viruses replicate their genome in the nucleus of infected cells. As a result, newly-synthesized vRNA genomes, in the form of viral ribonucleoprotein complexes (vRNPs), must be exported to the cytoplasm for productive infection. To characterize the composition of vRNP export complexes and their interplay with the nucleus of infected cells, we affinity-purified tagged vRNPs from biochemically fractionated infected nuclei. After treatment of infected cells with leptomycin B, a potent inhibitor of Crm1-mediated export, we isolated vRNP export complexes which, unexpectedly, were tethered to the host-cell chromatin with very high affinity. At late time points of infection, the cellular export receptor Crm1 also accumulated at the same regions of the chromatin as vRNPs, which led to a decrease in the export of other nuclear Crm1 substrates from the nucleus. Interestingly, chromatin targeting of vRNP export complexes brought them into association with Rcc1, the Ran guanine exchange factor responsible for generating RanGTP and driving Crm1-dependent nuclear export. Thus, influenza viruses gain preferential access to newly-generated host cell export machinery by targeting vRNP export complexes at the sites of Ran regeneration
Cross-sectional and prospective associations between sleep, screen time, active school travel, sports/exercise participation and physical activity in children and adolescents
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