Development of Molecular Markers Tightly Linked to Pvr4 Gene in Pepper Using Next-Generation Sequencing

It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding site–leucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable for large-scale molecular breeding programmes

Plant density in production of mini lettuce cultivars in organic system management

Production of mini vegetables in organic system is a good alternative to improve profit, but there are no researches about the optimum plant density for these cultivars in Brazilian conditions. Two experiments were conducted to evaluate the production of mini lettuce cultivars in different plant densities. Experiment 1 was conducted from January 1th (sowing) to February 10th (harvesting), 2012. The experimental design was completely randomized blocks, with six treatments in factorial scheme, 3 mini lettuce cultivars (Tudela, Renoir and Sartre) x 2 spacing between plants (16 and 20 cm), with eight replications and plots (2.04 m(2)) with six rows, spaced 15 cm. Experiment 2 was conducted from June 6th (sowing) to July 18th (harvesting), 2012. The cultivars Sartre and Renoir were evaluated under four plant densities (444,444; 333,333; 266,667 and 200,000 plants ha(-1), corresponding to spacing of 15x15, 15x20, 25x15 and 25x20 cm, respectively). Eight treatments were defined by a factorial scheme 2 (cultivars) x 4 (plant densities) and arranged in a completely randomized block design, with nine replications and plots with 2.04 m2. The evaluated characteristics in both experiments were total and marketable fresh weight per plant, plant dry weight, plant diameter and height, marketable yield and discard percentage. In first experiment, during the summer, cultivar Sartre showed the highest marketable fresh weight (72 g plant(-1)). Heaviest plants (91.6 g plant(-1)) were obtained with the higher plant spacing, but the highest yield (2.51 kg m(-2)) was obtained with the smaller spacing. In winter, plants with higher total (190 g plant(-1)) and marketable (146 g planta(-1)) fresh weight were obtained with cultivar Sartre, and the same was observed in low plant density. However, the higher plant density, the higher the yield.A produção de mini hortaliças em sistema orgânico é uma boa alternativa para aumentar os lucros do produtor; no entanto, não há pesquisas sobre a melhor densidade de plantio para estas cultivares nas condições brasileiras. Dois experimentos foram conduzidos para avaliar a produção de cultivares de mini alface em diferentes densidades de plantio. O experimento 1 foi conduzido de 1 de janeiro (semeadura) a 10 de fevereiro (colheita) de 2012. O delineamento experimental utilizado foi blocos ao acaso, com seis tratamentos em esquema fatorial, 3 cultivares de mini alface (Tudela, Renoir e Sartre) x 2 espaçamentos entre plantas (16 e 20 cm), com oito repetições e parcelas (2,04 m2 ) com seis linhas espaçadas em 15 cm. O experimento 2 foi conduzido de 6 de junho (semeadura) a 18 de julho (colheita) de 2012. As cultivares Sartre e Renoir foram avaliadas em quatro densidades de plantio (444.444, 333.333, 266.667 e 200.000 plantas ha-1, correspondendo aos espaçamentos, 15x15, 15x20, 25x15 e 25x20 cm, respectivamente). Os oito tratamentos foram definidos pelo esquema fatorial 2 (cultivares) x 4 (densidades de plantas) e dispostos em blocos ao acaso, com nove repetições e parcelas com 2,04 m2 . As características avaliadas em ambos experimentos foram massas de matéria fresca total e comercial, massa de matéria seca, diâmetro e altura das plantas, produtividade comercial e porcentagem de descarte. No primeiro experimento, durante o verão, a cultivar Sartre apresentou maior massa da matéria fresca comercial (72 g planta-1). Foram obtidas plantas mais pesadas (91,6 g planta-1) no maior espaçamento, porém, a maior produtividade (2,51 kg m-2) foi obtida com o menor espaçamento. No inverno, foram obtidas plantas com maiores massa fresca total (190 g planta-1) e comercial (146 g planta-1) na cultivar Sartre, assim como nas menores densidades de plantio. Porém, quanto maior a densidade, maior a produtividade.Universidade Estadual Paulista, Departamento de Horticultura, Faculdade de Ciências Agronômicas de Botucat

Ação conjunta de citocinina, giberelina e auxina em pimentão enxertado e não enxertado sob cultivo protegido

O objetivo deste trabalho foi avaliar o efeito da utilização do bioestimulante Stimulate® em plantas de pimentão enxertadas e não enxertadas, em ambiente protegido, no aumento de produção. Utilizou-se o delineamento experimental de blocos casualizados, no esquema fatorial 2 x 6, referente a plantas enxertadas e pé-franco e seis doses do Stimulate® (0; 75; 100; 125; 150 e 175 mL p.c. 100 L H2O-1), com quatro repetições. Realizaram-se sete aplicações foliares, quinzenais, do bioestimulante a partir do início do florescimento das plantas e avaliou-se o número total de frutos comerciais produzidos, comprimento, calibre, espessura de parede, massa média e produtividade final, totalizando treze colheitas. As plantas enxertadas foram mais produtivas em relação às plantas pé-franco, produzindo frutos de características agronômicas superiores. No geral, 100 e 125-150 mL p.c. 100 L H2O-1 de Stimulate® promovem incremento na produção de pimentão enxertado e pé-franco, respectivamente.The objective of this study was to evaluate the effect of using the Stimulate® biostimulant in grafted and non grafted plants of sweet pepper in a protected environment, to increase production. The experimental design was randomized blocks in a factorial scheme 2 x 6, referring to the grafted and non grafted plants and six doses of Stimulate® (0; 75; 100; 125; 150 and 175 mL p.c. 100 L H2O-1), with four replications. Seven foliar applications of the biostimulant were carried out fortnightly, from the beginning of the plants flowering and the total number of marketable fruits, length, diameter, wall thickness, average weight and final yield were evaluated, totalling thirteen harvests. The grafted plants were more productive than the non-grafted ones, producing fruits with better agronomic characteristics. In general, 100 and 125-150 mL p.c. 100 L H2O-1 of Stimulate® promoted an increase in the production of grafted and non-grafted sweet pepper plants, respectively

Abandonment of nicotine dependence treatment: A cohort study

CONTEXT AND OBJECTIVE: Non-adherence to treatment is one of the hindering factors in the process of smoking cessation. This study aimed to compare sociodemographic characteristics, smoking status and motivation among smokers who maintained or abandoned treatment to stop smoking, and to analyze associations between sociodemographic factors and smoking. DESIGN AND SETTING: Cohort study on 216 smokers who were attended at healthcare units in Cuiabá, Mato Grosso. METHODS: The instruments used were the Fagerström, URICA and CAGE questionnaires. Data from the initial evaluation was analyzed using the two-proportion test (&#945; < 0.05). The patients were monitored for six months and those who abandoned treatment were accounted for. Bivariate analysis was conducted, using crude prevalence ratios and 5% significance level (P < 0.05), with abandonment of treatment as the outcome variable. Associations with P < 0.20 were selected for multiple robust Poisson regression (RPa). RESULTS: The abandonment rate was 34.26%. Males and individuals in the 20-39 age group, in employment, with low motivation, with shorter time smoking and lower tobacco intake predominated in the dropout group. In the final model, gender (RPa 1.47; 95% CI: 1.03-2.10) and age group (RPa 3.77; 95% CI: 1.47-9.67) remained associated with abandonment. CONCLUSION: Males and individuals in the 20-39 age group, in employment, with low motivation, with shorter time smoking and lower tobacco intake more frequently abandoned the treatment. Male gender and younger age group were associated with abandonment of nicotine dependence treatment