The burden and risk factors of Sexually Transmitted Infections and Reproductive Tract Infections among pregnant women in Zimbabwe

<p>Abstract</p> <p>Background</p> <p>Sexually transmitted infections (STIs) and Reproductive tract infections (RTIs) are responsible for high morbidity among women. We aim to quantify the magnitude of the burden and risk factors of STI/RTI s among pregnant women in Zimbabwe.</p> <p>Methods</p> <p>A cross sectional study of pregnant women enrolled at 36 weeks of gestation from the national PMTCT program. Study was conducted from three peri-urban clinics around Harare Zimbabwe offering maternal and child health services.</p> <p>Results</p> <p>A total of 691 pregnant women were enrolled. Prevalence of HSV was (51.1%), HIV (25.6%) syphilis (1.2%), <it>Trichomonas vaginalis </it>(11.8%), bacterial vaginosis (32.6%) and Candidiasis (39.9%). Seven percent of the women had genital warts, 3% had genital ulcers and 28% had an abnormal vaginal discharge. Prevalence of serological STIs and vaginal infections were 51% and 64% respectively.</p> <p>Risk factors for a positive serologic STI were increasing age above 30 years, polygamy and multigravid; adjusted OR (95% CI) 2.61(1.49-4.59), 2.16(1.06-4.39), 3.89(1.27-11.98) respectively, partner taking alcohol and number of lifetime sexual partners. For vaginal infections it was age at sexual debut; OR (95% CI) 1.60(1.06-2.42). More than 25% of the women reported previous STI treatment. Fifty two percent reported ever use of condoms and 65% were on oral contraceptives. Mean age gap for sexual partners was 6.3 years older.</p> <p>Conclusions</p> <p>There is a high morbidity of STI/RTIs in this cohort. There is need to continuously screen, counsel, treat and monitor trends of STI/RTIs to assess if behaviour changes lead to reduction in infections and their sustainability.</p

Towards personalised allele-specific CRISPR gene editing to treat autosomal dominant disorders

Abstract CRISPR/Cas9 holds immense potential to treat a range of genetic disorders. Allele-specific gene disruption induced by non-homologous end-joining (NHEJ) DNA repair offers a potential treatment option for autosomal dominant disease. Here, we successfully delivered a plasmid encoding S. pyogenes Cas9 and sgRNA to the corneal epithelium by intrastromal injection and acheived long-term knockdown of a corneal epithelial reporter gene, demonstrating gene disruption via NHEJ in vivo. In addition, we used TGFBI corneal dystrophies as a model of autosomal dominant disease to assess the use of CRISPR/Cas9 in two allele-specific systems, comparing cleavage using a SNP-derived PAM to a guide specific approach. In vitro, cleavage via a SNP-derived PAM was found to confer stringent allele-specific cleavage, while a guide-specific approach lacked the ability to distinguish between the wild-type and mutant alleles. The failings of the guide-specific approach highlights the necessity for meticulous guide design and assessment, as various degrees of allele-specificity are achieved depending on the guide sequence employed. A major concern for the use of CRISPR/Cas9 is its tendency to cleave DNA non-specifically at “off-target” sites. Confirmation that S. pyogenes Cas9 lacks the specificity to discriminate between alleles differing by a single base-pair regardless of the position in the guide is demonstrated

Evaluating the significance of event and post-event sediment dynamics in a first order tributary using multiple sediment budgets.

Investigations of sediment transfer in upland catchments are rarely conducted over a sustained period of time, consequently a full understanding of the changing nature of sediment supply, storage and yield is often lacking. Three recent sediment budget studies from the Wet Swine Gill headwater catchment in the Lake District, Northern England, UK (a 0.65 km2, first-order tributary), provide evidence of changes in sediment transfer dynamics over the period 2002–2008. The first sediment budget in 2002 describes the impact of a hillslope debris slide and channelised debris flow event, where the former was the dominant budget component. The termination of the debris flow in the Wet Swine Gill channel meant that the vast majority of slide failure material was not transferred to the downstream fluvial system. However, subsequent modification of exposed hillslope sediment by post-event erosion processes and gully development resulted in ongoing erosion. A second sediment budget (June 2003–January 2004) demonstrated sediment yield downstream of the in-channel debris slide deposits far exceeds upstream fluvial sediment delivery by two orders of magnitude (c. 4,000 kg and c. 20 kg, respectively). Erosion of sediment from the exposed hillslope failure scar (c. 1300 kg) was less than channel erosion (c. 3300 kg), and sediment transfers from both the hillslope and channel sediment sources are sensitive to high-magnitude, low-frequency trigger events including summer thunderstorms, and winter rainfall/ snow-melt events. However, linear regression analysis only demonstrates weak or insignificant relations between meteorological conditions and sediment yield. A final sediment budget in April 2008 shows the significance of both hillslope (inclusive of gullying) and channel erosion/ transfer processes over the six-year monitoring period. In this budget, like the first sediment budget, the hillslope system is marginally more dominant, and therefore demonstrates a further switch in the relative significance of hillslope and channel system components. When interpreting such findings the potential uncertainty in the budget components, particularly in the unmeasured residual components, should be considered, as the magnitude of the error can be large. These results suggest that contemporary event and post-event sediment flux in small headwater catchments are more complex than short-term investigations would initially suggest. Furthermore there is a clear need for continued, longer-term monitoring of sediment system dynamics and associated hydro-meteorological conditions, in order to develop understanding of how future climate change may impact upland sediment systems