11 research outputs found
Determination of the physical environment within the Chlamydia trachomatis inclusion using ion-selective ratiometric probes
Full text linkChlamydia trachomatis is an obligate intracellular bacterium with a biphasic life cycle that takes place entirely within a membrane-bound vacuole termed an inclusion. The chlamydial inclusion is non-fusogenic with endosomal or lysosomal compartments but intersects a pathway involved in transport of sphingomyelin from the Golgi apparatus to the plasma membrane. The physical conditions within the mature chlamydial inclusion are unknown. We used ratiometric imaging with membrane-permeant, ion-selective fluorescent dyes for microanalyis of the physical environment within the inclusion. Determination of H + , Na + , K + and Ca 2 + concentrations using CFDA (carboxy fluorescein diacetate) or BCECF-AM (2 ′ ,7 ′ -bis (2-carboxyethyl)-5,6-carboxyfluorescein acetoxymethyl ester, SBFI-AM, PBFI-AM and fura-PE3-acetomethoxyester (Fura-PE3-AM), respectively, indicated that all ions assayed within the lumenal space of the inclusion approximated the concentrations within the cytoplasm. Stimulation of purinergic receptors by addition of extracellular ATP triggered a dynamic Ca 2 + response that occurred simultaneously within the cytoplasm and interior of the inclusion. The chlamydial inclusion thus appears to be freely permeable to cytoplasmic ions. These results have implications for nutrient acquisition by chlamydiae and may contribute to the non-fusogenicity of the inclusion with endocytic compartments.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72253/1/j.1462-5822.2002.00191.x.pd
Simple Method for Adapting DNA Fragments and PCR Products to All of the Commonly Used Restriction Sites
No full textMammalian Expression Vector with Two Multiple Cloning Sites for Expression of Two Foreign Genes
No full textThe autophagic pathway is actively modulated by phase II Coxiella burnetii to efficiently replicate in the host cell
No full textMaturation of the Coxiella burnetii parasitophorous vacuole requires bacterial protein synthesis but not replication
No full textMUC1-specific immune therapy generates a strong anti-tumor response in a MUC1-tolerant colon cancer model
No full textEvidence that Dot-dependent and -independent factors isolate the Legionella pneumophila phagosome from the endocytic network in mouse macrophages
Full text linkPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74415/1/j.1462-5822.2001.00093.x.pd
Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356
No full textGuidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
No full text[[sponsorship]]生物化學研究所[[note]]已出版;[SCI];有審查制度;具代表性[[note]]http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Drexel&SrcApp=hagerty_opac&KeyRecord=1554-8627&DestApp=JCR&RQ=IF_CAT_BOXPLO
