Optical Density Of Bone Repair After Implantation Of Homogenous Demineralized Dentin Matrix In Diabetic Rabbits

This research evaluated the bone repair process after implantation of homogenous demineralized dentin matrix (HDDM) in surgical defects in the parietal bone of rabbits with alloxan-induced diabetes, using a polytetrafluorethylene (PTFe) barrier for guided bone regeneration. Thirty-six rabbits were used and divided into four groups: control (C, n = 12), diabetic (D, n = 12, left parietal bone), diabetic with PTFe (DPTFe, same 12 rabbits, right parietal bone), and diabetic with PTFe associated to HDDM (D-PTFe+HDDM, n = 12). Bone defects were created in the parietal bone of the rabbits and the experimental treatments were performed, where applicable. The rabbits were sacrificed after 15, 30, 60 and 90 days. The bone defects were examined radiographically and by optical density (ANOVA and Tukey test, p < .05). The radiographic findings showed that the D-PTFe+HDDM group presented greater radiopacity and better trabecular bone arrangement when compared to that of the C, D and D-PTFe groups. The statistical analysis showed significant differences in the optical density of the newly formed bone among the studied groups. It was possible to conclude that HDDM was biocompatible in diabetic rabbits.223275280Abreu, P.P., Morosolli, A., Araújo, M.M., Carvalho, V.A.P., Gomes, M.F., Effects of autogenous demineralized dentin matrix on dental socket wound healing process in human (2004) Braz Oral Res, 18, p. 52. , SupplCarvalho, V.A.P., Tosello, D.O., Salgado, M.A.C., Gomes, M.F., Histomorphometric analysis of homogenous demineralized dentin matrix as osteopromotive material in rabbit mandibules (2004) Int J Oral Maxillofac Implants, 19 (5), pp. 679-686Gomes, M.F., Anjos, M.J.S., Nogueira, T.O., Catanzaro-Guimarães, S.A., Autogenous demineralized dentin matrix for tissue engineering applications: Radiographic and histomorphometric studies (2002) Int J Oral Maxillofac Implants, 17 (4), pp. 488-497Gomes, M.F., Silva, M.J.S., Nogueira, T.O., Catanzaro-Guimarães, S.A., Histologic evaluation of the osteoinductive property of autogenous demineralized dentin matrix on surgical bone defects in rabbit skulls using human amniotic membrane for guided bone regeneration (2001) Int J Oral Maxillofac Implants, 16 (4), pp. 563-571Guyton, A.C., Hall, J.E., (2006) Insulin, Glucagon, and Diabetes mellitus, pp. 827-840. , Guyton AC, Hall JE. Textbook of Medical Physiology. 11th ed, Elsevier;Fiorellini, J.P., Nevins, M.L., Norkin, A., Weber, H.P., Karimbux, N.Y., The effect of insulin therapy on osseointegration in a diabetic rat model (1999) Clin Oral Implants Res, 10 (5), pp. 362-368Lu, H., Kraut, D., Gerstenfeld, L.C., Graves, D.T., Diabetes interferes with the bone formation by affecting the expression of transcription factors that regulate osteoblast differentiation (2003) Endocrinology, 144 (1), pp. 346-352Nevins, M.L., Karimbux, N.Y., Weber, H.P., Giannobile, W.V., Fiorellini, J.P., Wound healing around endosseous implants in experimental diabetes (1998) Int J Oral Maxillofac Implants, 13 (5), pp. 620-629Claro, F.A., Lima, J.R., Salgado, M.A.C., Gomes, M.F., Porous Polyethylene for tissue engineering applications in diabetic rats treated with calcitonin: Histomorphometric analysis (2005) Int J Oral Maxillofac Implants, 20 (2), pp. 211-219Catanzaro-Guimarães, S.A., Catanzaro Guimarães, B.P., Garcia, R.B., Alle, N., Osteogenic potential of autogenic demineralized dentin implanted in bony defects in dogs (1986) Int J Oral Maxillofac Surg, 15 (2), pp. 160-169Bessho, K., Tagawa, T., Murata, M., Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction (1990) J Oral Maxillofac Surg, 48 (2), pp. 162-169Nakashima, M., Induction of dentine in amputated pulp of dogs by recombinant human bone morphogenetic proteins-2 and -4 with collagen matrix (1994) Arch Oral Biol, 39 (12), pp. 1085-1089Catanzaro-Guimarães SA. Possibility to reinforce bone repair with decalcified dentin matrix. In: Gesellschaft für orale Implantologie (ed.). Jahrbuch für Orale Implatologie. Berlin: Quintessenz1993. p. 33-

Biochemical approaches on commercial strains of Agaricus subrufescens growing under two environmental cultivation conditions

In the present work, the effect of the cultivation process, in the field and under a con- trolled environment, on biochemical parameters by using commercial strains of A. subrufescens were evaluated. The results obtained revealed that the strains cultivated in the field presented higher levels for most of the parameters evaluated (organic acids (20.5–48.0 g/100 g dw), tocopherols (107.0–198.6 μg/100 g dw), and phenolic acids and related compounds (245.2–359.0 μg/100 g dw and 10.6–23.7 μg/100 g dw, respectively)), except for the carbohydrates (53.4–72.6 g/100 g dw), energetic value (373–380 Kcal/100 g dw), and total free sugars (28.8–43.1 g/100 g dw), parameters in which the strains grown in a controlled environment present better results. For both cultivation systems, similar results were obtained regarding saturated, monounsaturated, and polyunsaturated fatty acids, as well as antioxidant and antimicrobial activities. These data contribute to the knowledge and highlight the characterized strains and the cultivation process, which can be used to obtain ingredients with potential applicability as a source of functional compounds.This research was funded by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP no. 19/00419-8 and 2018/21492-2). The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); national funding by FCT, P.I., through the institutional scientific employment program-contract for A.F., C.P. and L.B. contracts. This work is funded by the European Structural and Investment Funds (FEEI) through the Regional Operational Program North 2020, within the scope of Project Mobilizador ValorNatural®.info:eu-repo/semantics/publishedVersio

AVALIAÇÃO DOS EFEITOS DE DIFERENTES CONCENTRAÇÕES DE CLORO NA DESCAPSULAÇÃO E ECLOSÃO DE LARVAS DE ARTEMIA (Artemia sp.)

This paper was prepared in the Aquaculture Laboratory of the Federal University of the State of Parana, Brazil, with the purpose of determining the best sodium hypochloride (NaClO with 6% active ingredient) dosage to be employed in the artemia oocysts hatching process.The artemia oocysts were hydrated for 1 hour, filtered and submitted to a 10-minute chlorine treatment. The chlorine dosages tested were the following (NaClO - 6% sodium hypochloride): 0.38g, 0.75g, 1.50g, 3.00g and 6.00g of active ingredient per liter of water. Soon after chlorine application the oocysts were rinsed to eliminate the chlorine and immediately the oocysts were put into transparent plastic vials with water in the constant aeration and luminosity, where they remained for 53 hours. For the nauplii count one mililiter of the vial was put into a test tube with an interconnected aeration. Three counts were made for all repetitions and the threshold analysed was the number of hatched larvae per mililiter of hatching solution. The results were submitted to a normality test and it was determined that the hatching results did not show a normal pattern, therefore, it was not possible to use those averages as a comparison basis between treatments. Subsequently non-parametric tests were performed using the overall average as a comparison basis between treatments.Techically one can see the superiority of treatment with 1.50g of active chlorine, which resulted in the highest hatching-out counts, with 50% of the observed data presented 140 larvae hatched per milimiter.O presente trabalho foi realizado no Laboratório de Aqüicultura da Universidade Federal do Paraná (UFPR), com o objetivo de determinar a melhor dose de hipoclorito de sódio (NaClO com 6% de ingrediente ativo) a ser empregada na descapsulação de oocistos de artemia. Os oocistos de artemia foram hidratados durante 1 hora, filtrados e submetidos aos tratamentos com cloro durante 10 minutos. As dosagens de cloro testadas foram as seguintes (NaClO - hipoclorito de sódio 6%): 0,38g, 0,75g, 1,50g, 3,00g e 6,00g de ingrediente ativo por litro de água. Depois da aplicação do cloro fez-se a lavagem dos oocistos para eliminar o cloro aplicado e logo após os oocistos foram colocados na água em frascos plásticos transparentes com aeração e luminosidade constante onde ficaram durante 53 horas. Para contagem dos náuplios foi pipetado um mililitro do frasco com aeração ligada. Para todas as repetições foram feitas três contagens e o parâmetro analisado foi o número de larvas eclodidas por mililitro da solução de eclosão. Os resultados foram submetidos a um teste de normalidade e foi verificado que os resultados de eclosão não tinham distribuição normal, portanto as médias não puderam ser usadas como termo de comparação entre os tratamentos. Então foram realizados testes não-paramétricos que usam a mediana como parâmetro de comparação entre os tratamentos. Tecnicamente observa-se a superioridade do tratamento de 1,50g de cloro ativo que apresentou o ponto máximo de eclosão, tendo 50% dos dados de eclosão ao redor de 140 larvas eclodidas por mililitro

Emerging Enteropathogenic Escherichia coli Strains?

Escherichia coli strains of nonenteropathogenic serogroups carrying eae but lacking the enteropathogenic E. coli adherence factor plasmid and Shiga toxin DNA probe sequences were isolated from patients (children, adults, and AIDS patients) with and without diarrhea in Brazil. Although diverse in phenotype and genotype, some strains are potentially diarrheagenic