Analysis of the influence of the different times of polymerization by incandescent light in composite resins by evaluation of its abrasive wear

No mercado atual a qualidade é necessária na fabricação dos produtos. No mercado odontológico, as resinas compostas são cada vez mais utilizadas para substituir materiais tradicionais, como amalgama, nas restaurações estéticas e funcionais, apresentando boa estética e funcionalidade. A composição química destes materiais é variável e os resultados das reações químicas do processo de polimerização são bastante desconhecidos. O objetivo deste trabalho era medir a resistência do desgaste abrasivo de seis resinas compostas vendidas no mercado brasileiro polimerizadas com tempos de 10, 20 e 40 segundos. O método do disco retificado modificado para aplicações em odontologia foi usado para tornar esta proposta viável. Neste banco de ensaios um disco dinâmico, revestido por porcelana, desgasta um disco estático revestido do tipo de resina que se pretende avaliar, que foi polimerizada durante um tempo pré-estabelecido. Com a determinação do desgaste de material, num determinado tempo, determinava-se a agressividade (capacidade de um material para desgastar outro material) do disco dinâmico sobre o disco estático. Utilizando-se para isso um programa computacional (LabView). Posteriormente era feita a regressão linear dos valores obtidos e determinado o coeficiente angular, utilizado no cálculo de agressividade. Valores elevados de agressividade indicam uma maior agressão do disco dinâmico contra o disco estático. Os resultados mostraram um comportamento variável para cada material que foi polimerizado nos diferentes tempos de polimerização utilizado, mostrando que o método para fazer estudos de desgaste é prático e rápido para testar materiais novos antes da sua utilização.1317787Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Não temIn the current market an increasing quality is demanded to manufacture products. At the odontologic market the composed resins are becoming more and more used to replace old materials, like amalgamates in the aesthetic and functional restorations, since they present a better appearance and easier manuscript. The chemical composition of these materials varies and the results of the chemical reactions of polymerization processes are quite unknown. The aim of this work was to measure the resistance of the abrasive wear of six composed resins sold in Brazilian market when displayed to polymerization periods of 10, 20 and 40 seconds. The grinding disc method appropriated for odontology was used to make this process possible. For this a bank of assays in which a porcelain covered dynamic disc consumes a static disc recovered by the polymerized resin is used to demonstrate the method in a pre-established time. The abrasive wear was determined by the calculation of the aggressiveness (capacity of a material to consume another). This aggressiveness is determined by an initial graph of displacement for time, through the computational method (LabView program). After they have passed through a linear regression the results give as a possibility the angular coefficient, considered a parameter at the calculation of aggressiveness. Higher values of aggressiveness which means a higher aggression suffered by the resin disc indicate worse resistance to the abrasive consuming of this resin in relation to porcelain. The results showed a variable comportment for each material ahead the different polymerization time, which prove the importance in create a practical and fast analyses method to test new materials before their us

Photosynthesis, stomatal conductance and leaf water potential in crabwood (Carapa guianensis)

Leaf water potential is one of the most important factors affecting stomatal functioning. The aim of this study was to assess the effect of variation in diurnal irradiance and vapour pressure deficit on photosynthesis (A), stomatal conductance (g s) and leaf water potential (psi) in Carapa guianensis (Aubl.). Data were collected from 07:00 to 17:00 h. Photosynthetic rates reached a maximum (2.5 µmol m-2 s-1) at 10:00 h, thereafter declined to a minimum of 1 µmol m-2 s-1 at 16:00 h. Stomatal conductance oscillated during the day, from 0.04 mol m-2 s-1 (at midday) to 0.02.mol.m-2.s-1 at the end of the afternoon. Leaf water potential was higher early in the morning (-0.3 MPa) and lower (-0.75 MPa) at mid-afternoon (14:30 -15:00 h). After reaching a minimum, psi increased up to -0.64 MPa at sunset. Photosynthetic rates increased linearly as a function of g s (P < 0.01). Also there was a positive relationship between psi and g s (P< 0.01). Photosynthetic rates declined during the day after reaching a peak early in the morning, which makes clear that environmental factors that influence psi greatly affect carbon assimilation of C. guianensis.O potencial hídrico da folha é um dos fatores mais importantes que afetam o funcionamento dos estômatos. O objetivo deste trabalho foi avaliar o efeito da variação diurna na irradiância e déficit de pressão de vapor (DPV) na fotossíntese (A), condutância estomática (g s) e potencial hídrico da folha (psi) em Carapa guianensis (Aubl.). Os dados foram coletados de 07:00 às 17:00 h. A taxa fotossintética atingiu um valor máximo (2,5 µmol m-2 s-1) às 10:00 h, depois declinou até atingir um mínimo de 1 µmolm-2 s-1 às 16:00 h. A condutância estomática oscilou durante o dia, de 0,04 molm-2s-1 (ao meio dia) para 0,02 molm-2s- 1 no final da tarde. O potencial hídrico da folha foi máximo nas primeiras horas do dia (-0,3 MPa) e mínimo (-0,75 MPa) no meio da tarde (14:30 a 15:00 h). Após ter alcançado um mínimo, o psi aumentou até -0,64 MPa no fim da tarde. A taxa fotossintética aumentou linearmente em função do g s (P < 0,01). Também houve uma relação positiva entre psi e g s (P < 0,01). A taxa fotossintética declinou durante o dia após ter alcançado um pico no início da manhã, demonstrando que os fatores ambientais que afetam o psi têm efeito significativo na assimilação do carbono de C. guianensis

Differentially expressed plasmatic microRNAs in Brazilian patients with Coronavirus disease 2019 (COVID-19): preliminary results

Background: Coronavirus disease 2019 (COVID-19) is caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is known that host microRNAs (miRNAs) can be modulated to favor viral infection or to protect the host. Herein, we report preliminary results of a study aiming at identifying differentially expressed plasmatic miRNAs in Brazilian patients with COVID-19. Methods and results: miRNAs were extracted from the plasma of eight patients with COVID-19 (four patients with mild COVID-19 and four patients with severe/critical COVID-19) and four healthy controls. Patients and controls were matched for sex and age. miRNA expression levels were detected using high-throughput sequencing. Differential miRNA expression and enrichment analyses were further evaluated. A total of 18 miRNAs were differentially expressed between patients with COVID-19 and controls. miR-4433b-5p, miR-6780b-3p, miR-6883-3p, miR-320b, miR-7111-3p, miR-4755-3p, miR-320c, and miR-6511a-3p were the most important miRNAs significantly involved in the PI3K/AKT, Wnt/β-catenin, and STAT3 signaling pathways. Moreover, 42 miRNAs were differentially expressed between severe/critical and mild patients with COVID-19. miR-451a, miR-101-3p, miR-185-5p, miR-30d-5p, miR-25-3p, miR-342-3p, miR-30e-5p, miR-150-5p, miR-15b-5p, and miR-29c-3p were the most important miRNAs significantly involved in the Wnt/β-catenin, NF-κβ, and STAT3 signaling pathways. Conclusions: If validated by quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in a larger number of participants, the miRNAs identified in this study might be used as possible biomarkers for the diagnosis and severity of COVID-19