Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway

Carotenoids protect the photosynthetic apparatus against harmful radicals arising from the presence of both light and oxygen. They also act as accessory pigments for harvesting solar energy, and are required for stable assembly of many light-harvesting complexes. In the phototrophic bacterium Rhodobacter (Rba.) sphaeroides phytoene desaturase (CrtI) catalyses three sequential desaturations of the colourless carotenoid phytoene, extending the number of conjugated carbon–carbon double bonds, N, from three to nine and producing the yellow carotenoid neurosporene; subsequent modifications produce the yellow/red carotenoids spheroidene/spheroidenone (N = 10/11). Genomic crtI replacements were used to swap the native three-step Rba. sphaeroides CrtI for the four-step Pantoea agglomerans enzyme, which re-routed carotenoid biosynthesis and culminated in the production of 2,2′-diketo-spirilloxanthin under semi-aerobic conditions. The new carotenoid pathway was elucidated using a combination of HPLC and mass spectrometry. Premature termination of this new pathway by inactivating crtC or crtD produced strains with lycopene or rhodopin as major carotenoids. All of the spirilloxanthin series carotenoids are accepted by the assembly pathways for LH2 and RC–LH1–PufX complexes. The efficiency of carotenoid-to-bacteriochlorophyll energy transfer for 2,2′-diketo-spirilloxanthin (15 conjugated Cdouble bond; length as m-dashC bonds; N = 15) in LH2 complexes is low, at 35%. High energy transfer efficiencies were obtained for neurosporene (N = 9; 94%), spheroidene (N = 10; 96%) and spheroidenone (N = 11; 95%), whereas intermediate values were measured for lycopene (N = 11; 64%), rhodopin (N = 11; 62%) and spirilloxanthin (N = 13; 39%). The variety and stability of these novel Rba. sphaeroides antenna complexes make them useful experimental models for investigating the energy transfer dynamics of carotenoids in bacterial photosynthesis

The Energy of Regular Black Hole in General Relativity Coupled to Nonlinear Electrodynamics

According to the Einstein, Weinberg, and M{\o}ller energy-momentum complexes, we evaluate the energy distribution of the singularity-free solution of the Einstein field equations coupled to a suitable nonlinear electrodynamics suggested by Ay\'{o}n-Beato and Garc\'{i}a. The results show that the energy associated with the definitions of Einstein and Weinberg are the same, but M{\o}ller not. Using the power series expansion, we find out that the first two terms in the expression are the same as the energy distributions of the Reissner-Nordstr\"{o}m solution, and the third term could be used to survey the factualness between numerous solutions of the Einstein field eqautions coupled to a nonlinear electrodynamics.Comment: 11 page

A Measurement of Psi(2S) Resonance Parameters

Cross sections for e+e- to hadons, pi+pi- J/Psi, and mu+mu- have been measured in the vicinity of the Psi(2S) resonance using the BESII detector operated at the BEPC. The Psi(2S) total width; partial widths to hadrons, pi+pi- J/Psi, muons; and corresponding branching fractions have been determined to be Gamma(total)= (264+-27) keV; Gamma(hadron)= (258+-26) keV, Gamma(mu)= (2.44+-0.21) keV, and Gamma(pi+pi- J/Psi)= (85+-8.7) keV; and Br(hadron)= (97.79+-0.15)%, Br(pi+pi- J/Psi)= (32+-1.4)%, Br(mu)= (0.93+-0.08)%, respectively.Comment: 8 pages, 6 figure

Measurements of the Mass and Full-Width of the ηc\eta_c Meson

In a sample of 58 million $J/\psi$ events collected with the BES II detector, the process J/$\psi\to\gamma\eta_c$ is observed in five different decay channels: $\gamma K^+K^-\pi^+\pi^-$, $\gamma\pi^+\pi^-\pi^+\pi^-$, $\gamma K^\pm K^0_S \pi^\mp$ (with $K^0_S\to\pi^+\pi^-$), $\gamma \phi\phi$ (with $\phi\to K^+K^-$) and $\gamma p\bar{p}$. From a combined fit of all five channels, we determine the mass and full-width of $\eta_c$ to be $m_{\eta_c}=2977.5\pm1.0 ({stat.})\pm1.2 ({syst.})$ MeV/$c^2$ and $\Gamma_{\eta_c} = 17.0\pm3.7 ({stat.})\pm7.4 ({syst.})$ MeV/$c^2$.Comment: 9 pages, 2 figures and 4 table. Submitted to Phys. Lett.

Measurement of Branching Ratios for ηc\eta_c Hadronic Decays

In a sample of 58 million $J/\psi$ events collected with the BES II detector, the process J/$\psi\to\gamma\eta_c$ is observed in five decay channels: $\eta_c \to K^+K^-\pi^+\pi^-$, $\pi^+\pi^-\pi^+\pi^-$, $K^\pm K^0_S \pi^\mp$ (with $K^0_S\to\pi^+\pi^-$), $\phi\phi$ (with $\phi\to K^+K^-$) and $p\bar{p}$. From these signals, we determine $Br(J/\psi\to\gamma\eta_c)\times Br(\eta_c\to K^+K^-\pi^+\pi^-)$ $=(1.5\pm0.2\pm0.2)\times10^{-4}$, $Br(J/\psi\to\gamma\eta_c)\times Br(\eta_c\to \pi^+\pi^-\pi^+\pi^-)$ $=(1.3\pm0.2\pm0.4)\times10^{-4}$, $Br(J/\psi\to\gamma\eta_c)\times Br(\eta_c\to K^\pm K_{S}^{0}\pi^\mp)$ $=(2.2\pm0.3\pm0.5)\times10^{-4}$, $Br(J/\psi\to\gamma\eta_c)\times Br(\eta_c\to \phi\phi)$ $=(3.3\pm0.6\pm0.6)\times10^{-5}$ and $Br(J/\psi\to\gamma\eta_c)\times Br(\eta_c\to p\bar{p})$ $=(1.9\pm0.3\pm0.3)\times10^{-5}$.Comment: 8 pages, 1 figures and 4 table. Submitted to Phys. Lett.

Evidence of psi(3770) non-DD-bar Decay to J/psi pi+pi-

Evidence of $\psi(3770)$ decays to a non-${D \bar D}$ final state is observed. A total of $11.8 \pm 4.8 \pm 1.3$ \psi(3770) \to \PPJP events are obtained from a data sample of 27.7 $\rm {pb^{-1}}$ taken at center-of-mass energies around 3.773 GeV using the BES-II detector at the BEPC. The branching fraction is determined to be BF(\psi(3770) \to \PPJP)=(0.34\pm 0.14 \pm 0.09)%, corresponding to the partial width of \Gamma(\psi(3770) \to \PPJP) = (80 \pm 33 \pm 23) keV.Comment: 8 pages, 7 figures, Submitted to Physics Letters

Use of SMS texts for facilitating access to online alcohol interventions: a feasibility study

A41 Use of SMS texts for facilitating access to online alcohol interventions: a feasibility study In: Addiction Science & Clinical Practice 2017, 12(Suppl 1): A4