130 research outputs found

    Wavy stripes and squares in zero P number convection

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    A simple model to explain numerically observed behaviour of chaotically varying stripes and square patterns in zero Prandtl number convection in Boussinesq fluid is presented. The nonlinear interaction of mutually perpendicular sets of wavy rolls, via higher mode, may lead to a competition between the two sets of wavy rolls. The appearance of square patterns is due to the secondary forward Hopf bifurcation of a set of wavy rolls.Comment: 8 pages and 3 figures, late

    Epithelial argininosuccinate synthetase is dispensable for intestinal regeneration and tumorigenesis

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    The epithelial signaling pathways involved in damage and regeneration, and neoplastic transformation are known to be similar. We noted upregulation of argininosuccinate synthetase (ASS1) in hyperproliferative intestinal epithelium. Since ASS1 leads to de novo synthesis of arginine, an important amino acid for the growth of intestinal epithelial cells, its upregulation can contribute to epithelial proliferation necessary to be sustained during oncogenic transformation and regeneration. Here we investigated the function of ASS1 in the gut epithelium during tissue regeneration and tumorigenesis, using intestinal epithelial conditional Ass1 knockout mice and organoids, and tissue specimens from colorectal cancer patients. We demonstrate that ASS1 is strongly expressed in the regenerating and Apc-mutated intestinal epithelium. Furthermore, we observe an arrest in amino acid flux of the urea cycle, which leads to an accumulation of intracellular arginine. However, loss of epithelial Ass1 does not lead to a reduction in proliferation or increase in apoptosis in vivo, also in mice fed an arginine-free diet. Epithelial loss of Ass1 seems to be compensated by altered arginine metabolism in other cell types and the liver.Cellular mechanisms in basic and clinical gastroenterology and hepatolog
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