Fine-needle Aspiration Biopsy For Breast Lesions: A Comparison Between Two Devices For Obtaining Cytological Samples

Context and Objective: Fine-needle aspiration biopsy has been accepted worldwide for breast lesions. However, some questions remain, including the appropriateness of the puncture method. The objective of this work was to compare aspirates obtained by the auto-vacuum device and by the syringe pistol holder. Design and Setting: Randomized trial for validation of diagnostic method, at Hospital das Clinicas do Universidade Federal de Goiás and Hospital Araújo Jorge, Goiânio. Methods: 351 patients presenting breast lumps underwent fine-needle aspiration biopsy, either with the auto-vacuum device or the syringe pistol holder. A single cytopathologist analyzed all of the cytology slides. The rates of insufficient material, cellularity, cell distortion and background hemorrhage were evaluated. Results: The percentages of insufficient material were 16% and 22% (p = 0.18), for the auto-vacuum and pistol aspirates, respectively. Good cellularity was seen in 34% of autovacuum and 29% of pistol samples (p = 0.4). Cell distortion was seen in 31 and 26 cases, respectively (p = 07). Background hemorrhage occurred in 63 (35%) and 54 cases (31%) (p = 0.2), for auto-vacuum and pistol. The sensitivity was 88% and 86%; specificity 99% and 100%, positive predictive value 96% and and 100%, negative predictive value 96% and 95% and total occuracy 76% and 75% for the auto-vacuum and pistol, respectively. Conclusion: The results obtained from the two fine-needle aspiration biopsy methods were equivalent. Therefore, the auto-vacuum device is a good option for obtaining aspirates for cytology.1236271276Catania, S., Ciatto, S., Breast cytology: Instruments and technique (1992) Breast Cytology in Clinical Practice, pp. 11-60. , In: Ciatto S, editor. London: Martin DunitzDe Freitas Jr., R., Hamed, H., Fentiman, I., Fine needle aspiration cytology of palpable breast lesions (1992) Br J Clin Pract, 46 (3), pp. 187-190Franzen, S., Zajicek, J., Aspiration biopsy in diagnosis of palpable lesions of the breast. Critical review of 3479 consecutive biopsies Acta (1968) Radiol Ther Phys Biol, 7 (4), pp. 241-262Souza, J.A., Freitas Jr., R., Moreita, M.A.R., Philocreon, G.R., Acurácia da sensação tátil da PAAF no diagnóstico de less̄ocs mamárias (1995) Rev Bras Mastologia, 5 (3), pp. 7-10Freitas Júnior, R., Hamed, H., Millis, R.R., Tomas, N.M.A., Fentiman, I.S., Fine needle aspiration of breast lesions using auto-vacuum dispositive (1996) Rev Bras Mastologia, 6 (3), pp. 126-128de Freitas Júnior, R., Giraldo, P.C., Rerrori, O., Vieira-Matos, A.N., Tambascia, J.K., Fine needle aspiration biopsy of solid tumours by auto-vacuum system: A study in rats (1992) Eur J Surg Oncol, 18 (6), pp. 605-607Boccato, P., How to treat the aspirated material (1992) Breast Cytology in Clinical Practice, pp. 61-64. , In: Ciatto, S, editor London: Martin Dunitz(1987) International Union Against Cancer, , UICC. TNM classification of malignant tumors. Berlin: Springer-Verlag(1982) Am J Clin Pathol, 78 (6), pp. 806-816. , The World Health Organization Histological Typing of Breast Tumors - Second Edition. The World OrganizationGelabert, H.A., Hsiu, J.G., Mullen, J.T., Jaffe, A.H., D'Amato, N.A., Prospective evaluation of the rate of fine-needle aspiration biopsy in the diagnosis and management of patiencs with palpable solid breast lesions (1990) Am Surg, 56 (4), pp. 263-267Rouquayrol, M.Z., Epidemiologia e saúde (1988), Rio de Janeiro: Medsi - Editora CientíficaRoberts, J.C., Rainsbury, R.M., 'Tactile sensation: A new clinical sign during fine needle aspiration of breast lumps Ann (1994) R Coll Surg Eng, 76 (2), pp. 136-138Henderson, M.A., McCready, D.R., A simple technique for fine needle aspiration cytology (1994) J Am Coll Surg, 179 (4), pp. 471-473Sasaki, J., Izu, K., Automatic fine needle aspiration pistol specially designed for small tumors (1998) Acta Cytol, 42 (3), pp. 829-830Tao, L.C., Smith, J.W., Fine-needle aspiration biopsy using a newly-developed pencil-grip syringe holder (1999) Diagn Cytopathol, 20 (2), pp. 99-104Parel, J.J., Garrell, P.C., Smallwood, J.A., Fine needle aspiration cytology of breast masses: An evaluation of its accuracy and reasons for diagnostic failure (1987) Ann R Coll Surg Engl, 69 (4), pp. 156-159Abele, J., Stanley, M.W., Rollnis, S.D., Miller, T.R., What constitutes an adequate smear in fine-needle aspiration cytology of the breast? (1998) Cancer, 84 (1), pp. 57-61Zajdela, A., Ghossein, N.A., Pilleron, J.P., Ennuyer, A., The value of aspiration cytology in the diagnosis of breast cancer: Experience at the Fondation Curie (1975) Cancer, 35 (2), pp. 499-506Layfield, L.J., Dodd, L.G., Cytologically low grade malignancies: An important interpretative pitfall responsible for false negative diagnoses in fine-needle aspiration of the breast (1996) Diagn Cytopathol, 15 (3), pp. 250-259Feichter, G.E., Haberthür, F., Gobat, S., Dalquen, P., Breast cytology. Statistical analysis and cytohistologic correlations (1997) Acta Cytol, 41 (2), pp. 327-332Arisio, R., Cuccorese, C., Accinelli, G., Mano, M.P., Bordon, R., Fessia, L., Role of fine-needle aspiration biopsy in breast lesions: Analysis of a series of 4,110 cases (1998) Diagn Cytopathol, 18 (6), pp. 462-467Ariga, R., Bloom, K., Reddy, V.B., Fine-needle aspiration of clinically suspicious palpable breast masses with histopathologic correlation (2002) Am J Surg, 184 (5), pp. 410-41

CARACTERÍSTICAS DE CULTURAS LÁCTICAS PROBIÓTICAS PARA USO EM PRODUTOS CÁRNEOS FERMENTADOS: SENSIBILIDADE AOS SAIS DE CURA E USO DE ANTIBIÓTICOS PARA CONTAGEM SELETIVA

Os objetivos deste trabalho foram avaliar a resistência de espécies probióticas de Lactobacillus na presença de sais de cura e testar sua sensibilidade frente a diferentes antimicrobianos para o desenvolvimento de meio de cultura seletivo. As culturas Lactobacillus casei, Lactobacillus paracasei e Lactobacillus rhamnosus foram semeadas em ágar MRS contendo concentrações de 0% a 3% de cloreto de sódio e 0 a 200 ppm de nitrito de sódio. O efeito do uso concomitante dos sais de cura foi verificado utilizando-se 3% de cloreto de sódio e 200 ppm de nitrito de sódio. As bactérias probióticas e a cultura starter Pediococcus pentosaceus foram testadas frente a 20 discos de antimicrobianos pela técnica de disco-difusão. O crescimento dos probióticos não apresentou alteração nas diferentes concentrações de cloreto de sódio, assim como nas concentrações de até 200 ppm de nitrito de sódio. Verificou-se resistência ao uso simultâneo de cloreto e nitrito de sódio. Entre os antimicrobianos testados, a tetraciclina apresentou resultados satisfatórios para a inibição dos probióticos permitindo o crescimento isolado da cultura starter. Os probióticos apresentaram desenvolvimento satisfatório na presença dos sais de cura, demonstrando potencial para sua utilização em produtos cárneos fermentados com ação probiótica. CHARACTERISTICS OF PROBIOTIC CULTURES FOR THE USE IN FERMENTED MEAT PRODUCTS - SENSIBILITY TO CURING SALTS AND ANTIBIOTIC USE FOR THE SELECTIVE ENUMERATION Abstract The objective of this study was to evaluate the resistance of probiotic species of Lactobacillus in the presence of curing salts and to test their sensibility in the presence of antibiotics for the development of a selective culture media. The probiotic cultures, Lactobacillus casei, Lactobacillus paracasei spp paracasei and Lactobacillus casei spp rhamnosus, were plated in MRS agar with concentration of 0 to 3% of sodium chloride and 0 to 200 ppm of sodium nitrite. The inhibitory effect of 3% sodium chloride and 200 ppm sodium nitrite in simultaneous use was evaluated for the probiotic strains. The sensibility of the starter culture, Pediococcus pentosaceus and the probiotic cultures in the presence of antibiotic was carried out using 20 different antibiotic discs by the disc-diffusion technique. The growth of the probiotic cultures wasnt affected at different concentrations of sodium chloride, and even to concentrations of 200 ppm of sodium nitrate. Resistance was verified by simultaneous use of sodium chloride and nitrate. Among the tested antibiotics, tetracycline showed a satisfactory inhibition effect for the probiotic strains, since the growth of starter Pediococcus pentosaceus was not affected. The probiotics showed satisfactory growth in the presence of curin

A Spectral Bernstein Theorem

We study the spectrum of the Laplace operator of a complete minimal properly immersed hypersurface $M$ in $\R^{n+1}$. (1) Under a volume growth condition on extrinsic balls and a condition on the unit normal at infinity, we prove that $M$ has only essential spectrum consisting of the half line $[0, +\infty)$. This is the case when $\lim_{\tilde{r}\to +\infty}\tilde{r}\kappa_i=0$, where $\tilde{r}$ is the extrinsic distance to a point of $M$ and $\kappa_i$ are the principal curvatures. (2) If the $\kappa_i$ satisfy the decay conditions $|\kappa_i|\leq 1/\tilde{r}$, and strict inequality is achieved at some point $y\in M$, then there are no eigenvalues. We apply these results to minimal graphic and multigraphic hypersurfaces.Comment: 16 pages. v2. Final version: minor revisions, we add Proposition 3.2. Accepted for publication in the Annali di Matematica Pura ed Applicata, on the 05/03/201

Supersymmetric Monojets at the Large Hadron Collider

Supersymmetric monojets may be produced at the Large Hadron Collider by the process qg -> squark neutralino_1 -> q neutralino_1 neutralino_1, leading to a jet recoiling against missing transverse momentum. We discuss the feasibility and utility of the supersymmetric monojet signal. In particular, we examine the possible precision with which one can ascertain the neutralino_1-squark-quark coupling via the rate for monojet events. Such a coupling contains information on the composition of the neutralino_1 and helps bound dark matter direct detection cross-sections and the dark matter relic density of the neutralino_1. It also provides a check of the supersymmetric relation between gauge couplings and gaugino-quark-squark couplings.Comment: 46 pages, 10 figures. The appendix has been rewritten to correct an error that appears in all previous versions of the appendix. This error has no effect on the results in the main body of the pape

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A crescente demanda por motores elétricos capazes de operar em altas velocidades, com boa produção de conjugado, tem tornado os motores de relutância síncronos competitivos e bastante empregados em aplicações veiculares, indústrias têxteis e em sistemas de bombeamento e ventilação. Em função disso, este artigo apresenta as características de projeto, modelo matemático do motor e do controle de velocidade, empregando a técnica de controle de campo orientado indireto (IFOC). Os resultados, por fim, são analisados a partir de simulação computacional

Genetic transformation of Citrus sinensis with Citrus tristeza virus (CTV) derived sequences and reaction of transgenic lines to CTV infection

Transgenic Citrus sinensis (L.) Osb. plants, cvs. Valencia and Hamlin, expressing Citrus tristeza virus (CTV) derived sequences were obtained by genetic transformation. The gene constructs were pCTV-CP containing the 25 kDa major capsid protein gene (CTV-CP), pCTV-dsCP containing the same CTV-CP gene in an intron-spliced hairpin construct, and pCTV-CS containing a 559 nt conserved region of the CTV genome. The transgenic lines were identified by PCR and the transgene integration was confirmed by Southern blot. Transgene mRNA could be detected in most transgenic lines containing pCTV-CP or pCTV-CS transgene. The mRNA of pCTV-dsCP transgene was almost undetectable, with very light bands in most analyzed plants. The transgene transcription appears to be closely linked to the type of gene construct. The virus challenge assays reveals that all transgenic lines were infected. However, it was possible to identify propagated clones of transgenic plants of both cultivars studied with a low virus titer, with values similar to the non-inoculated plants (negative control). These results suggested that the transgenic plants present some level of resistance to virus replication. The higher number of clones with low virus titer and where mRNA could not be detected or was presented in a very light band was found for pCTV-dsCP-derived transgenic lines561162166CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informaçã

Tense and aspect in word problems about motion: diagram, gesture, and the felt experience of time

© 2014, Mathematics Education Research Group of Australasia, Inc. Word problems about motion contain various conjugated verb forms. As students and teachers grapple with such word problems, they jointly operationalize diagrams, gestures, and language. Drawing on findings from a 3-year research project examining the social semiotics of classroom interaction, we show how teachers and students use gesture and diagram to make sense of complex verb forms in such word problems. We focus on the grammatical category of “aspect” for how it broadens the concept of verb tense. Aspect conveys duration and completion or frequency of an event. The aspect of a verb defines its temporal flow (or lack thereof) and the location of a vantage point for making sense of this durational process