Addressee factor in Text of the Official Document (Decrees of Governor of Tula Region, Issued in Connection with the Spread of Covid-2019)

The issue of taking into account the addressee factor in the text of an official document is considered. The controversial nature of this issue and the topicality of the material determined the relevance of the study. It is proved that the originality of the texts of decrees is defined by two oppositely directed tendencies. On the one hand, it is stated that the addressee factor is taken into account due to the performative nature of the legislator’s speech. It is noted that his speech activity is based primarily on prohibitions and restrictions; permissions are exceptions to the prohibition; the requirements are reduced to the obligations of the addressee and the instructions to him. It was revealed that the implicit meanings of the text become clear taking into account the pragmatic presupposition “what is appropriate is good”. It was established that the implicit assessment is formed under the influence of the addressee factor: the preambles of decrees are estimated, the temporal characteristics of the statements are detailed. It is shown that when constructing texts, the focus on the addressee becomes obvious due to the structural-rubric allocation and grouping of information. It is emphasized that the genre canons are being blurred due to the desire of the legislator for convincing reasoning of decisions. On the other hand, the analysis of texts in dynamics revealed that the historically established system of changing documents is not focused on the addressee. It is concluded that the complexity of the presentation of information or its vagueness testifies to the stereotypes of the legislator’s speech behavior

Information technologies in organization of medical aid to patients with vascular diseases of the brain

The article presents results of the implementation activities in the hospital №18 of Ufa with a 'Program monitoring rendering of medical aid to patients with vascular diseases of the brain', which is part of the Unified medical information system. The program is compatible with hospital register primary vascular Department and allows you to optimize continuity in the monitoring patients at all stages of medical care.В статье представлены результаты внедрения в деятельность больницы №18 г. Уфы «Программы мониторинга оказания медицинской помощи пациентам с сосудистыми заболеваниями головного мозга», входящей в состав Единой медицинской информационной системы. Программа совместима с госпитальным регистром первичного сосудистого отделения и позволяет оптимизировать преемственность в наблюдении за пациентами на всех этапах оказания медицинской помощи

Deadly liaisons: fatal attraction between CCN matricellular proteins and the tumor necrosis factor family of cytokines

Recent studies have revealed an unexpected synergism between two seemingly unrelated protein families: CCN matricellular proteins and the tumor necrosis factor (TNF) family of cytokines. CCN proteins are dynamically expressed at sites of injury repair and inflammation, where TNF cytokines are also expressed. Although TNFα is an apoptotic inducer in some cancer cells, it activates NFκB to promote survival and proliferation in normal cells, and its cytotoxicity requires inhibition of de novo protein synthesis or NFκB signaling. The presence of CCN1, CCN2, or CCN3 overrides this requirement and unmasks the apoptotic potential of TNFα, thus converting TNFα from a proliferation-promoting protein into an apoptotic inducer. These CCN proteins also enhance the cytotoxicity of other TNF cytokines, including LTα, FasL, and TRAIL. Mechanistically, CCNs function through integrin α6β1 and the heparan sulfate proteoglycan (HSPG) syndecan-4 to induce reactive oxygen species (ROS) accumulation, which is essential for apoptotic synergism. Mutant CCN1 proteins defective for binding α6β1-HSPGs are unable to induce ROS or apoptotic synergism with TNF cytokines. Further, knockin mice that express an α6β1-HSPG-binding defective CCN1 are blunted in TNFα- and Fas-mediated apoptosis, indicating that CCN1 is a physiologic regulator of these processes. These findings implicate CCN proteins as contextual regulators of the inflammatory response by dictating or enhancing the cytotoxicity of TNFα and related cytokines

Complete Structural Model of Escherichia coli RNA Polymerase from a Hybrid Approach

A combination of structural approaches yields a complete atomic model of the highly biochemically characterized Escherichia coli RNA polymerase, enabling fuller exploitation of E. coli as a model for understanding transcription

Changing climate both increases and decreases European river floods

Climate change has led to concerns about increasing river floods resulting from the greater water-holding capacity of a warmer atmosphere. These concerns are reinforced by evidence of increasing economic losses associated with flooding in many parts of the world, including Europe. Any changes in river floods would have lasting implications for the design of flood protection measures and flood risk zoning. However, existing studies have been unable to identify a consistent continental-scale climatic-change signal in flood discharge observations in Europe, because of the limited spatial coverage and number of hydrometric stations. Here we demonstrate clear regional patterns of both increases and decreases in observed river flood discharges in the past five decades in Europe, which are manifestations of a changing climate. Our results—arising from the most complete database of European flooding so far—suggest that: increasing autumn and winter rainfall has resulted in increasing floods in northwestern Europe; decreasing precipitation and increasing evaporation have led to decreasing floods in medium and large catchments in southern Europe; and decreasing snow cover and snowmelt, resulting from warmer temperatures, have led to decreasing floods in eastern Europe. Regional flood discharge trends in Europe range from an increase of about 11 per cent per decade to a decrease of 23 per cent. Notwithstanding the spatial and temporal heterogeneity of the observational record, the flood changes identified here are broadly consistent with climate model projections for the next century, suggesting that climate-driven changes are already happening and supporting calls for the consideration of climate change in flood risk management

Molecular signatures for CCN1, p21 and p27 in progressive mantle cell lymphoma

Mantle cell lymphoma (MCL) is a comparatively rare non-Hodgkin’s lymphoma characterised by overexpression of cyclin D1.Many patients present with or progress to advanced stage disease within 3 years. MCL is considered an incurable disease withmedian survival between 3 and 4 years. We have investigated the role(s) of CCN1 (CYR61) and cell cycle regulators inprogressive MCL. We have used the human MCL cell lines REC1 G519 > JVM2 cells by RQ-PCR, depicting a decrease in CCN1expression with disease progression. Investigation of CCN1 isoform expression by western blotting showed that whilst expres-sion of full-length CCN1 was barely altered in the cell lines, expression of truncated forms (18–20 and 28–30 kDa) decreasedwith disease progression. We have then demonstrated that cyclin D1 and cyclin dependent kinase inhibitors (p21CIP1and p27KIP1)are also involved in disease progression. Cyclin D1 was highly expressed in REC1 cells (OD: 1.0), reduced to one fifth in G519cells (OD: 0.2) and not detected by western blotting in JVM2 cells. p27KIP1followed a similar profile of expression as cyclin D1.Conversely, p21CIP1was absent in the REC1 cells and showed increasing expression in G519 and JVM2 cells. Subcellularlocalization detected p21CIP1/p27KIP1primarily within the cytoplasm and absent from the nucleus, consistent with altered roles in treatment resistance. Dysregulation of the CCN1 truncated forms are associated with MCL progression. In conjunction withreduced expression of cyclin D1 and increased expression of p21, this molecular signature may depict aggressive disease andtreatment resistance