Assessment of physical conditions and proposed best management practices of domestic storage tanks supplied by a water utility in a rapidly growing city

The physical conditions of domestic storage tanks for customers supplied by a water utility were assessed in Kampala, a rapidly growing City in Uganda. A longitudinal assessment of 372 storage tanks in 6 sampled administrative Wards with a minimum of 6 samples collected from each site in both wet (March-May) and dry (June-August) months of 2017 was carried out. A set of guiding questions were used to establish tank conditions with a YES or NO response and a range of low to critical risk rating. It was revealed that there was a statistically significant relationship (p=0.001) between tank physical conditions and quality of stored water. Two of six Wards in the City had high levels of water contamination related to domestic water storage tanks with poor sanitary conditions and contaminated water with E. coli. The study therefore revealed that the physical conditions and management of domestic water storage tanks have an effect on water quality. This is important information for a water utility as it means that it is not enough to supply safe water if the quality may deteriorate upon storage at the consumer premises. A routine inspection checklist and consumer guidelines for domestic storage tank management are proposed

Application of a yeast-based assay protocol developed to monitor total oestrogenic activity induced by 17&#233-oestradiol in activated sludge supernatants from batch experiments

Batch experiments were carried out with activated sludge from laboratory reactors and a full-scale treatment plant spiked with 17β-oestradiol (E2). An oestrogen-sensitive yeast-based assay protocol, described in detail in a related publication, was used to measure reduction of E2-induced total oestrogenic activity from the sludge supernatant over a 15 d period after which the sludge was re-spiked to check for possible enhancement of reduction by pre-exposed sludge during an additional 15 d period. The reduction was generally improved by increasing sludge solids concentrations and by continuous mixing. For a 100 ngE2/ℓ spike there was >40% reduction of oestrogenic activity within 15 d, which improved to >70% by pre-exposing the sludge. The oestrogenic activity produced by a dose of 100 μgE2/ℓ was readily removed by most sludges within 15 d. However, re-spiking the activated sludge with the same E2 concentration caused some sludges to lose reduction capacity. Water SA Vol.32 (3) 2006: pp.355-36

Household-level Fluoride reduction from drinking water using crushed fired clay – proof of concept

Residents of Bunyangabu District in Uganda have reportedly suffered from cases of dental fluorosis due to consumption of water with high levels of Fluoride from Ntabago Stream. A household-level sand filter incorporating crushed fired clay as an adsorbent was designed for a household to reduce the Fluoride concentration in their drinking water from the natural raw water levels (2-3 mgF/L) to permissible, healthy levels (0.5-1.0 mg/L). Pieces of fired clay bricks were crushed, and particles of 150 μm to 300 μm in size were selectively obtained by sieving. Stream water was filtered through replicate model layered filter columns of the prepared clay, sand (fine sand of 150 μm-2 mm; coarse sand of 2-5 mm), and gravel (6-15 mm). It was found out that fired clay layers of 2.5 and 5 cm thick reduce the concentration of Fluoride in water by up to 74%, to less than 1.0 mg/L. This was deduced by evaluating Fluoride concentration in both the clay and water before and after filtration, using the SPADNS Colorimetric method. Use of crushed fired clay for reduction in Fluoride concentration in drinking water is the focus of this paper. Additional work will optimise filter design to improve overall water quality

Assessment of physical conditions and proposed best management practices of domestic storage tanks supplied by a water utility in a rapidly growing City.

This is a research article on the physical conditions of domestic storage tanks for customers supplied by a water utility was assessed in Kampala, a rapidly growing City in UgandaThe physical conditions of domestic storage tanks for customers supplied by a water utility were assessed in Kampala, a rapidly growing City in Uganda. A longitudinal assessment of 372 storage tanks in 6 sampled administrative Wards with a minimum of 6 samples collected from each site in both wet (March-May) and dry (June-August) months of 2017 was carried out. A set of guiding questions were used to establish tank conditions with a YES or NO response and a range of low to critical risk rating. It was revealed that there was a statistically significant relationship (p=0.001) between tank physical conditions and quality of stored water. Two of six Wards in the City had high levels of water contamination related to domestic water storage tanks with poor sanitary conditions and contaminated water with E. coli. The study therefore revealed that the physical conditions and management of domestic water storage tanks have an effect on water quality. This is important information for a water utility as it means that it is not enough to supply safe water if the quality may deteriorate upon storage at the consumer premises. A routine inspection checklist and consumer guidelines for domestic storage tank management are proposed

Understanding children’s experiences of self-wetting in humanitarian contexts: An evaluation of the Story Book methodology

Little is known about how children in humanitarian contexts experience self-wetting. Children can wet themselves due to having the medical condition of urinary incontinence (the involuntary leakage of urine), or due to them not wanting to or not being able to use the toilet facilities available (social or functional incontinence). Self-wetting is a global public health challenge: the physical health of children can suffer; they can miss out on educational and social opportunities; they may face increased protection risks; and the emotional effect on daily life can be significantly negative. The Story Book methodology was developed to facilitate conversations with children aged five to eleven in humanitarian contexts (specifically refugee settlements in Adjumani District, Uganda; and refugee camps in Cox’s Bazar, Bangladesh) about self-wetting to understand how humanitarian professionals can best meet the needs of children that wet themselves. This paper has evaluated how far the Story Book methodology meets the specific requirements of conducting research a) in a humanitarian context; b) with young children; and c) on a personal and highly sensitive topic. Data has been used from Story Book sessions held with children in Adjumani District and Cox’s Bazar, and from semi-structured interviews held with adults known to have participated in the planning and/or facilitation of the sessions. The evaluation found that although the Story Book methodology provided deep insights into how children in humanitarian contexts experience self-wetting, it was not always implemented as designed; it is not practical to implement in humanitarian settings; and it was not acceptable to all participants and facilitators as a research tool. Changes have been recommended to improve the methodology as a research tool to better understand how children experience personal health issues, but even with such changes the methodology will remain better suited to non-humanitarian contexts

Amplifying local voices to reduce failure in the water, sanitation and hygiene sector

This is a poster briefWASH endeavours regularly fail. Sometimes this means that entire programmes do not achieve their stated aims, sometimes these failures are setbacks which can be rectified with sufficient reflection and action. This research aimed to develop an evidence base of how and why field-based WASH professionals in four sub-Saharan African countries believe failures occur, their experiences when sharing and discussing them within their organisations, and how they believe a culture conducive to publicly sharing and learning from failures could be nurtured

Developing a yeast-based assay protocol to monitor total oestrogenic activity induced by 17&#223-oestradiol in activated sludge supernatants from batch experiments

A yeast-based assay protocol developed for detecting oestrogenic activity in activated sludge (AS) supernatant is described. The protocol used Saccharomyces cerevisiae construct RMY/ER-ERE with human oestrogen receptor (ERα) and lacZ reporter genes, and was developed by modifying existing assays for use with AS samples from batch experiments. The method was able to detect total oestrogenic activity (without prior extraction) in supernatants of AS spiked with 17β-oestradiol (E2) with a detection limit of 0.03 ngE2-equivalent/ℓ and an overall quantification limit of 100 ngE2-equivalent/ℓ. Mean E2-induced oestrogenic activity recoveries of >56% were obtained from the spiked samples. Water SA Vol.32 (3) 2006: pp.345-35

Developing a Biosensor for Estrogens in Water Samples: Study of the Real-time Response of Live Cells of the Estrogen-sensitive Yeast Strain RMY/ER-ERE using Fluorescence Microscopy

Using a fluorescein di-{beta}-d-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17{beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 min of exposure to E2. This finding which agrees with the well-known rates of enzyme-catalyzed reactions could have implications for shortening the duration of environmental sample screening and monitoring regimes using yeast-based estrogen assays, and the development of biosensors for environmental estrogens to complement quantification methods