Study of Biological Properties of Effective Pesticides Destructor Pseudomonas Putida P2

A promising approach for remediation of contaminated soils is the use of biological products based on microorganisms-destructors. Of particular interest are the strains that can decompose several types of pollutants. Potential strains-destructors should be not only effective but also adapted for certain soil and climatic conditions. In addition, when introducing a significant amount of biological agents into the ecosystem, the lack of toxicological and pathogenic effects on plants, animals, and humans is particularly important. The object of the study is the destructor strain of sim-triazine herbicide of prometrin Pseudomonas putida P2. The study explores the ability of strain to use various pesticides as the only carbon source, the effect of bacterial culture on seed germination, biotesting of strain on toxicity using earthworms. Besides, the study presents the phytopathogenic factors and enzymes causing bacteria invasiveness into animals and humans. The determination of dehydrogenase activity of prometrin destructor strain shows that this strain can also use active ingredients of pesticide malathion, HCH, and 4,4-DDT as only sole carbon source. It is also established that the studied strain Pseudomonas putida P2 increases the germination of seeds of the variety Saratovskaya 70 and carrots of the variety Samson and does not possess pathogenicity factors. The strain biotesting on the earthworms revealed no acute toxicity of the culture. The analysis of data obtained from the model pollution shows that the introduction of the destructor of Prometrium Pseudomonas putida P2 into the soil can create favorable conditions for vital activity and reproduction of earthworms

WAYS TO REDUCE THE RISKS OF INFECTION WHILE TRAINING FOR WORK WITH MICROORGANISMS OF THE I–II GROUPS OF PATHOGENICITY (HAZARD)

Minimization of risks of infection when training the specialists to work with microorganisms – agents of particularly dangerous infectious diseases – is one of the key objectives for staff members of the Department for specialists training and curricula development at the FGHI RusRAPI “Microbe”. The paper discusses the ways to reduce the risks of infection with agents of particularly dangerous infectious diseases among the attendee and the tutors of qualification courses while studying how to work with this group of microorganisms. Outlined are priority areas: usage of attenuated, avirulent strains of the agents, as well as recombinant strains of nonpathogenic bacteria; exploitation of the state-of-the-art technical equipment; insure the skills of safe PBA handling and professionally significant traits of character in the process of realization of currently existing academic programs. Identified has been the optimum approach to the assessment of reliability and safety of professional activities among the personnel working with PBA of the I–II groups of hazard. Developed has been the algorithm for competence level evaluation in the specialists who are allowed to work with PBA of the I–II groups of hazard; job profile diagrams have been charted. Designed methods of expert evaluation of professionally significant skills and traits of the staff provide for personnel identification using a range of psychological tests and play an important role in vocational selection and orientation, both during the job placement and advanced training of the staff, as well as in the reduction of risks associated with the factor of human error

COLON CAPSULE ENDOSCOPY: POSSIBILITIES OF NON-INVASIVE DIAGNOSTICS OF COLON DISEASES

Purpose. Evaluation of feasibility of colon capsule endoscopy (CCE), as non-invasive method of diagnostics of colon abnormalities.Patients and methods. From 31.01.2014 to 10.01.2017 we successfully performed 100 CCE in 101 (99,0%) patients (m-50, f-48, mean age 44,7 ± 14,8 years, range 22–81), including 2 twins aged 8 years (CCE was performed with pediatrician). The main indication for colonoscopy was colorectal cancer screening (73 (73,0%) pts). The indication for colonoscopy to 2 twins was complicated Peutz Jeghers syndrome in one of them. CCE was performed using the system PillCam (Given Imaging) and capsule PillCam Colon 2. For the bowel preparation we used two-stage regimen; for the evaluation of preparation — J.A. Leighton scale.Results. Preparation was adequate in 88 (88,0%) pts, non-adequate — in 12 (12,0%) pts. Total CCE was performed in 82 (82,0%) patients. The mean colon transit time was 4 hours 43 min ± 3 hours15 min. Total evaluation of the colon was performed in 82 (82,0%) pts. Mucosal lesions were revealed in 75 (75,0%) pts, including epithelial polyps in 52 (69,3%) pts. We also revealed comorbid lesions in 41 (54,6%) pts. Colonoscopy after CCE was recommended to 28 (37,3%) pts because of polyp detection, sized ≥6 mm, and because of inflammatory changes of colon mucosa, but only 11 (39,3%) pts were performed colonoscopy.Conclusion. In this article the experience of colon capsule endoscopy demonstrates the principal possibility of noninvasive evaluation of the colon and detection of colon abnormalities

Superlong GRBs

We searched for anomalously long GRBs (GRBs) in the archival records of the Burst and Transient Sources Experiment (BATSE). Ten obvious superlong (>500 s) GRBs with almost continuous emission episodes were found. Nine of these events are known from the BATSE catalog, but five have no duration estimates; we found one burst for the first time. We also detected events with emission episodes separated by a long period of silence (up to 1000 s) with a total duration of 1000--2000 s. In the latter case, we cannot reach an unequivocal conclusion about a common origin of the episodes due to the BATSE poor angular resolution. However, for most of these pairs, the probability of a coincidence of independent GRBs is much lower than unity, and the probability that all of these are coincidences is 10E-8. All of the events have a hardness ratio (the ratio of the count rates in different energy channels) typical of GRBs, and their unique duration is unlikely to be related to their high redshifts. Superlong bursts do not differ in their properties from typical long (>2 s) GRBs. We estimated the fraction of superlong GRBs (>500 s) among the long GRBs in the BATSE sample with fluxes up to 0.1 ph cm^{-2} s^{-1} to be between 0.3 and 0.5%, which is higher than the estimate based on the BATSE catalog.Comment: 11 pages, 4 figures, 3 tables, translation is made by Astronomy Letter

Clinical-morphological parallels of the PNPLA3 gene polymorphism in patients with nonalcoholic fatty liver disease

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, and its detection in the general population has reached a global scale. Despite the fact that in the early stages the disease is characterized by a relatively mild period, the development during its natural course of nonalcoholic steatohepatitis, cirrhosis and hepatocellular carcinoma causes deterioration of long-term forecast. Growing evidence indicates that NAFLD is a complex, multifaceted etiology, involving many factors, including genetic. In the present review, we focused on the genetic component of NAFLD, namely, the role of the PNPLA3 gene polymorphism in the development and course of the disease, and States its progression, such as non-alcoholic steatohepatitis, liver cirrhosis and hepatocellular carcinoma

Using of New Technologies of Learning Physics in Modern University Education

Статья посвящена описанию результатов применения инновационных технологий при обучении физике студентов Уральского государственного экономического университета.The article is devoted to the description of the results of the application of innovative technologies in teaching physics to students of the Ural State University of Economics

Blended Educational Technologies in Higher Education: Systematic Review of Domestic Publications

The article presents the results of generalization and systematization of scientific and practical information on the variety of types of blended educational technologies used in Russian higher education institutions. This specific product of digitalization causes the greatest interest of researchers and practitioners in the field of education. This systematic review is based on national scientific publications related to the development and practical implementation of educational technologies in higher education. The authors have analyzed 180 papers published between 2016 and 2021 in peer-reviewed scientific journals approved by the Higher Attestation Commission. Methods of qualitative and quantitative content analysis, systems analysis and contextological analysis, as well as analytical grouping, have been used.Content analysis of publications showed that the main property of blended educational technologies is their variability, which manifests itself through the described system of indicators. However, the structural organization of blended educational technologies models remains the most debatable issue among researches.The authors have identified and described the main directions in the development of the theory and practical application of blended educational technologies, namely: the formation of the structure and boundaries of the conceptual and terminological field; elaboration of the methodological basis of blended education development and definition of theoretical basis for changing traditional learning systems by designing a learning process based on the mechanisms of pedagogical and digital technologies integration.It has been established that the empirical studies with the elements of experimental work focused on defining the most effective combinations of traditional and digital educational technologies dominate in the national scientific community. The most popular methods of such integration include rotation of forms and types of learning activity in traditional and digital learning environments, integration of digital and traditional educational technologies based on the Moodle learning management system, as well as inclusion of separate forms and means of e-learning in the educational process

Women’s experience of divorce. Acta biomedica scientifica

The article is devoted to the problem of studying the experience of women going through the situation of divorce at different stages of the divorce and post-divorce period.Methods. The study was carried out at the intersection of idiographic and nomothetic approaches. At the first stage, the specifics of the experience of experiencing divorce in the online space are described, revealed through an interpretive-phenomenological analysis of the content of a closed Internet forum (n = 297). On the second – the specifics of emotional perception, cognitive assessment and coping with the situation of divorce, depending on the post-divorce experience (n = 50).Results. The experience of divorce by women is accompanied by ambivalent emotions, activates the understanding of the situation and coping with it. Online communication is an effective strategy focused on getting support, self-change, designing the future, allowing you to experience a sense of community in a life situation while maintaining psychological safety. The assessment of the divorce situation and coping strategies are specific: women who have gone through a divorce less than 3 years ago are more likely to seek social support; from 3 to 10 years – prefer the strategy of “flight-avoidance”; more than 10 years ago – “positive revaluation”. Conclusion. The experience of women experiencing the situation of divorce, including emotional, cognitive and conative components, is specific depending on the stage of the divorce and post-divorce period. An effective mechanism for shaping the experience of experiencing a divorce is network communication in a closed group of people who have similar problems