Environmental enrichment has no effect on the development of dopaminergic and GABAergic fibers during methylphenidate treatment of early traumatized gerbils

It is widely believed, that environmental factors play a crucial role in the etiology and outcome of psychiatric diseases such as Attention-Deficit/Hyperactivity Disorder (ADHD). A former study from our laboratory has shown that both methylphenidate (MP) and handling have a positive effect on the dopaminergic fiber density in the prefrontal cortex (PFC) of early traumatized gerbils (Meriones unguiculatus). The current study was performed to investigate if enriched environment during MP application has an additional influence on the dopaminergic and GABAergic fiber densities in the PFC and amygdala in this animal model

Zur selbsttätig sicheren Begrenzung von nuklearer Leistung und Brennstofftemperatur in innovativen Kernreaktoren

Nuclear energy probably will not contribute significantly to the future worldwide energysupply until it can be made catastrophefree. Therefore it has to be shown, that theconsequences of even largest accidents will have no major impact to the environmentof a power plant.In this paper one of the basic conditions for such a nuclear technology is discussed.Using mainly the modular pebble-bed high-temperature reactor as an example, thedesign principles, analytical methods and the level of knowledge as given today incontrolling reactivity accidents by inherent safety features of innovative nuclear reactorsare described. Complementary possibilities are shown to reach this goal with systems ofdifferent types of construction . Questions open today and resulting requirements forfuture activities are discussed .Today's knowledge credibly supports the possibility of a catastrophefree nucleartechnology with respect to reactivity event

Cellular and Behavioral Effects of Cranial Irradiation of the Subventricular Zone in Adult Mice

Background: In mammals, new neurons are added to the olfactory bulb (OB) throughout life. Most of these new neurons, granule and periglomerular cells originate from the subventricular zone (SVZ) lining the lateral ventricles and migrate via the rostral migratory stream toward the OB. Thousands of new neurons appear each day, but the function of this ongoing neurogenesis remains unclear. Methodology/Principal Findings: In this study, we irradiated adult mice to impair constitutive OB neurogenesis, and explored the functional impacts of this irradiation on the sense of smell. We found that focal irradiation of the SVZ greatly decreased the rate of production of new OB neurons, leaving other brain areas intact. This effect persisted for up to seven months after exposure to 15 Gray. Despite this robust impairment, the thresholds for detecting pure odorant molecules and short-term olfactory memory were not affected by irradiation. Similarly, the ability to distinguish between odorant molecules and the odorant-guided social behavior of irradiated mice were not affected by the decrease in the number of new neurons. Only long-term olfactory memory was found to be sensitive to SVZ irradiation. Conclusion/Significance: These findings suggest that the continuous production of adult-generated neurons is involved i

Mice with Mutation in Dynein Heavy Chain 1 Do Not Share the Same Tau Expression Pattern with Mice with SOD1-Related Motor Neuron Disease

Due to controversy about the involvement of Dync1h1 mutation in pathogenesis of motor neuron disease, we investigated expression of tau protein in transgenic hybrid mice with Dync1h1 (so-called Cra1/+), SOD1G93A (SOD1/+), double (Cra1/SOD1) mutations and wild-type controls. Total tau-mRNA and isoforms 0, 1 and 2 N expression was studied in frontal cortex, hippocampus, spinal cord and cerebellum of presymptomatic and symptomatic animals (age 70, 140 and 365 days). The most significant differences were found in brain cortex and cerebellum, but not in hippocampus and spinal cord. There were less changes in Cra1/SOD1 double heterozygotes compared to mice harboring single mutations. The differences in total tau expression and in profile of its isoforms between Cra1/+ and SOD1/+ transgenics indicate a distinct pathogenic entity of these two conditions

Inhibition of Lassa Virus Glycoprotein Cleavage and Multicycle Replication by Site 1 Protease-Adapted α1-Antitrypsin Variants

The virus family Arenaviridae includes several hemorrhagic fever causing agents such as Lassa, Guanarito, Junin, Machupo, and Sabia virus that pose a major public health concern to the human population in West African and South American countries. Current treatment options to control fatal outcome of disease are limited to the ribonucleoside analogue ribavirin, although its use has some significant limitations. The lack of effective treatment alternatives emphasizes the need for novel antiviral therapeutics to counteract these life-threatening infections. Maturation cleavage of the viral envelope glycoprotein by the host cell proprotein convertase site 1 protease (S1P) is critical for infectious virion production of several pathogenic arenaviruses. This finding makes this protease an attractive target for the development of novel anti-arenaviral therapeutics. We demonstrate here that highly selective S1P-adapted α1-antitrypsins have the potential to efficiently inhibit glycoprotein processing, which resulted in reduced Lassa virus replication. Our findings suggest that S1P should be considered as an antiviral target and that further optimization of modified α1-antitrypsins could lead to potent and specific S1P inhibitors with the potential for treatment of certain viral hemorrhagic fevers

"Once through" cycles in the pebble bed HTR

In the pebble bed HTR the "Once Through" cycles achieve a favorable conservation of uranium resources due to their high burnup and due to the relatively low fissile inventory. A detailed study is given for cycles with highly enriched uranium and thorium, 20 % enriched uranium and thorium, and for the low (l, 8 %) enriched cycle. The recommended cycle is based on the known THTR fuel element in the Th/U {93 %) cycle. The variant with separate Seed elements and Breed elements presents the best pioneer in view of later recycling and thermal breeding. The minimum proliferation risk is achieved in the Th/U (20 %) cycle basing on the fuel element type of the AVR, due to the low amount and high denaturization of the disloaded plutonium

Brennstoffzyklen des Kugelhaufen-Hochtemperaturreaktors in der Computersimulation

Because of its physical properties, the pebble-bed reactor permits diverse variations in the design of the reactor, the fuel elements, the mode of operation and the fuel cycle. A system of computer programs (V. S. O. P.) makes it possible to set up computer models of these different versions and explicitly to follow the physical behaviour in the reactor and fuel cycle. On this basis, open and closed fuel cycles are analyzed and the reactor properties with use of low-enrichment, medium-enrichment and high-enrichment uranium and thorium are compared. Parametric studies with respect to burnup and to fuel charge in the pebbles form the basis for the selection of reference designs in the particular cycles. In addition, the fuel-cycle properties of small reactors for power plants with multiple modular reactors are compared with those of large plants. The cycles are compared from the viewpoint of fissile-material economy, neutron economy, financial economy, technical development expenses, qualification for sites close to the consumers resistance to proliferation, and long-term hazard potential of the waste. The differences which can be recognized in these comparisons are attributed to the different physical properties which are associated with the cycles and which become evident in the computer simulation