Impact of different Agents on the Efficacy of Codling Moth Granulovirus in Tank Mixtures

In the control of codling moth it is common to combine the granulovirus with other agents, especially fungicides, in spray application. Therefore the knowledge about the influence of these agents on the efficacy of the virus in tank mix is very important. Studies on this subject were part of a project supported by BMELV (German Federal Ministry for Food, Agriculture and Consumer protection) at the Institute for Biological Control of JKI in Darmstadt. The granulovirus of Cydia pomonella (L.) (CpGV) was mixed with 10 different agents at concentrations as applied in the field. After the exposure the virus activity was calculated from larval mortality determined in bioassays with neonates of a susceptible codling moth strain. Only two agents with a pH of 11 (sodium silicate (water glass) and calcium polysulfide (lime sulphur)) reduced the virulence of CpGV significantly

The Right of Publicity: A Matter of Privacy, Property, or Public Domain?

This article discusses the right of publicity, beginning in Part II with the difficulty in defining a legal right of publicity, and the resulting various legal doctrines upon which the right has been analyzed

Libel and Slander in Virginia

The law of libel and slander in Virginia is unsettled. Even defining the terms is difficult. While many states classify libel and slander as separate torts, in Virginia defamation encompasses them both. Moreover, there are two separate definitions of defamation per se in Virginia. There are also two types of malice which are applicable in defamation actions; and to make matters worse, one type of malice which actually is not malice at all is called actual malice

The Ethics of E-Mail

In many ways, communicating by e-mail and other forms of electronic transmission reflects a fundamentally different way of human interaction. Historians eventually will put this in perspective, but one could easily conclude that e-mails are essentially a “third way” for people to communicate

Private Discrimination Actions Filed in Federal Court: Nonsubstantive Matters Affecting Liability and Relief

Confusion regarding who may be held liable and what relief may be sought is evident in the inconsistent and conflicting decisions of the federal courts in private actions which charge unlawful discrimination under color of state law. The cause of the confusion has little to do with whether in fact the plaintiff has been the victim of discrimination but may be attributed to the piecemeal development of what may be termed nonsubstantive matters which nevertheless substantially affect the issues of liability and relief

PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens.

Large-scale genetic screens using CRISPR/Cas9 technology have emerged as a major tool for functional genomics. With its increased popularity, experimental biologists frequently acquire large sequencing datasets for which they often do not have an easy analysis option. While a few bioinformatic tools have been developed for this purpose, their utility is still hindered either due to limited functionality or the requirement of bioinformatic expertise. To make sequencing data analysis of CRISPR/Cas9 screens more accessible to a wide range of scientists, we developed a Platform-independent Analysis of Pooled Screens using Python (PinAPL-Py), which is operated as an intuitive web-service. PinAPL-Py implements state-of-the-art tools and statistical models, assembled in a comprehensive workflow covering sequence quality control, automated sgRNA sequence extraction, alignment, sgRNA enrichment/depletion analysis and gene ranking. The workflow is set up to use a variety of popular sgRNA libraries as well as custom libraries that can be easily uploaded. Various analysis options are offered, suitable to analyze a large variety of CRISPR/Cas9 screening experiments. Analysis output includes ranked lists of sgRNAs and genes, and publication-ready plots. PinAPL-Py helps to advance genome-wide screening efforts by combining comprehensive functionality with user-friendly implementation. PinAPL-Py is freely accessible at http://pinapl-py.ucsd.edu with instructions and test datasets