Cooperative effects in surfactant adsorption layers at water/alkane interfaces

In the present work, the properties of dodecyl dimethyl phosphine oxide (C12DMPO) at the water/decane interface are studied and compared with those obtained earlier at the interface to hexane. To simulate the interfacial behavior, a two-component thermodynamic model is proposed, which combines the equation of state and Frumkin isotherm for decane with the reorientation model involving the intrinsic compressibility for the surfactant. In this approach, the surface activity of decane is governed by its interaction with C12DMPO. The theory predicts the influence of decane on the decrease of the surface tension at a very low surfactant concentration for realistic values of the ratio of the adsorbed amounts of decane and surfactant. The surfactantrsquo;s distribution coefficient between the aqueous and decane phases is determined. Two types of adsorption systems were used: a decane drop immersed into the C12DMPO aqueous solution, and a water drop immersed into the C12DMPO solution in decane. To determine the distribution coefficient, a method based on the analysis of the transfer of C12DMPO between water and decane is also employed

Herpesvirus infection and infectious mononucleosis

In this review we present current information on various aspects of infectious mononucleosis in children. Based on the analysis of scientific literature highlights the major etiological factors that lead to the development of this disease. The paper describes the clinical and laboratory features of infectious mononucleosis caused by Epstein-Barr virus, cytomegalovirus, human herpes virus type VI. Presents literature data on the impact of herpes infection on the development of mononucleosis. Showing modern aspects of an integrated diagnosis of herpesvirus infection in infectious mononucleosis in children

ГЕРПЕС-ВИРУСНЫЕ ИНФЕКЦИИ И ИНФЕКЦИОННЫЙ МОНОНУКЛЕОЗ (ОБЗОР ЛИТЕРАТУРЫ)

In this review we present current information on various aspects of infectious mononucleosis in children. Based on the analysis of scientific literature highlights the major etiological factors that lead to the development of this disease. The paper describes the clinical and laboratory features of infectious mononucleosis caused by Epstein-Barr virus, cytomegalovirus, human herpes virus type VI. Presents literature data on the impact of herpes infection on the development of mononucleosis. Showing modern aspects of an integrated diagnosis of herpesvirus infection in infectious mononucleosis in children.В приведённом обзоре представлены современные сведения о различных аспектах инфекционного мононуклеоза у детей. На основании анализа научной литературы выделены основные этиологические факторы, приводящие к развитию данного заболевания. В статье отражены клинические и лабораторные особенности инфекционного мононуклеоза, вызванного вирусом Эпштейна – Барр, цитомегаловирусом, вирусом герпеса человека VI типа. Приведены литературные данные о влиянии герпетической инфекции на развитие мононуклеоза. Показаны современные аспекты комплексной диагностики герпес-вирусных инфекций при инфекционном мононуклеозе в детском возрасте

Biosafety model of adenovirus infection: Effects of bacterial proteases for infection of human cells in vitro

To determine the antiviral activity of various biologically active compounds, the model of adenovirus infection on the basis of cell cultures of human HEK293A and recombinant adenovirus Ad-EGFP, expressing green fluorescent protein EGFP. Adenoviruses have a capsid size of 70-90 nm and are able to infect dividing and nondividing cells in vitro and in vivo. Recombinant adenoviruses are the replicative defect in the cells of humans and animals. The developed model allowed us to determine the effect of bacterial proteases in the infected cell cultures with adenovirus. This model can also be used for screening drugs with potential protivivovirusnoy activity

Project and Research Work of Learners Within the Online Learning Module in the Alternative Educational Organization in the Conditions of Distance Learning: Problems and Opportunities

The article deals with the experience of organizing project and research activities of learners by means of the online learning module in the context of distance learning in the alternative educational organization International Child Center “Artek”. The peculiarities of work of learners using the project and research map “Navigator of Senses” in a distance format are considered. The article describes the essence and structure of the project and research map “Navigator of senses”.Представлен опыт организации проектно-исследовательской деятельности обучающихся посредством сетевого образовательного модуля в условиях дистанционного обучения в нетиповой образовательной организации «Международный детский центр “Артек”». Раскрыты особенности работы обучающихся в дистанционном формате с использованием проектно-исследовательской карты «Навигатор смыслов». Описаны сущность и структура проектно-исследовательской карты «Навигатор смыслов»

КЛИНИКО-ЛАБОРАТОРНЫЕ ОСОБЕННОСТИ ПАРВОВИРУСНОЙ ИНФЕКЦИИ У ДЕТЕЙ

The article is devoted to the study of clinical symptoms and laboratory changes at parvovirus B19 infection in children during the rise of the incidence of infectious erythema. 44 children ailing with laboratory confirmed parvovirus B19 infection aging from 2 months to 14 years were complexly examined. It was established that the disease occurs mainly in boys of preschool and early school age, with a seasonal rise in April and May. General infectious syndrome and syndrome of exanthema dominate the clinical picture. Infectious erythema has benign course.Статья посвящена изучению клинических симптомов и лабораторных изменений при парвовирусной инфекции В19 у детей в период подъема заболеваемости инфекционной эритемой. Проведено комплексное обследование 44 детей с подтвержденной лабораторно парвовирусной инфекцией В19 в возрасте от 2 месяцев до 14 лет. Установлено развитие заболевания преимущественно у детей дошкольного и раннего школьного возраста, чаще у мальчиков, с сезонным подъемом в апреле и мае. В клинической картине доминирует общеинфекционный синдром и синдром экзантемы. Инфекционная эритема имеет доброкачественное течение

Glutamyl endopeptidase of Bacillus intermedius strain 3-19. Purification, properties, and crystallization

A homogeneous glutamyl endopeptidase splitting peptide bonds of glutamic and, rarely, of aspartic acid residues in peptides and proteins was isolated from Bacillus intermedius 3-19 culture filtrate using chromatography on CM-cellulose and Mono S. The enzyme molecular mass is 29 kD and the p/ is 8.4. The proteinase is inhibited by DFP. The enzyme, like other glutamyl endopeptidases, reveals two pH optima (pH 7.5 and 9.0) for casein and one (pH 8.0) for Z-Glu-pNA hydrolysis. The Km for the hydrolysis of the latter substrate is 6 mM. The enzyme activity is optimal at 55°C. The enzyme is stable in the pH range 6.5-11.0. Its N-terminal sequence shows 56% coinciding residues when compared with that of Bacillus licheniformis glutamyl endopeptidase. Crystal prisms or plates 0.25-0.3 × 0.15 × 0.07-0.1 mm have been grown using the vapor diffusion technique in a hanging drop followed by macroseeding. The crystals belong to the space group B2 with the following unit cell parameters: a = 69.59 Å; b = 61.61 Å; c = 56.11 Å; γ = 117.57°. The X-ray data set to 1.7 Å resolution has been collected on an automatic synchrotron (EMBL Hamburg Station)

ПОРАЖЕНИЕ СЕРДЕЧНО-СОСУДИСТОЙ СИСТЕМЫ ПРИ ВИРУСНЫХ ИНФЕКЦИЯХ

Article is devoted to studying of the submitted epidemiological, clinical, tool, laboratory data on pathology of cardiovascular system at various viral infections. The review is based on results of domestic and foreign researches. At viral infections damage of heart and his carrying-out system perhaps as during the sharp period of a disease, and the period of a convalescence or at the chronic course of virus process. The greatest cardiothrogenism is possessed by enteroviruses, which affect the myocardium in 5–15% of cases. Much attention is paid to herpesviruses, widespread, persistently persistent in the body, as one of the reasons for the development of dilated cardiomyopathy, coronary vasculitis, early atherosclerosis, cardiac rhythm disturbance. Other infections that may affect the cardiovascular system include influenza viruses, adenovirus, poliovirus, Epstein-Barr virus, cytomegalovirus, human immunodeficiency virus, hepatitis, mumps, rubella, herpes simplex, varicella, arbovirus, respiratory-syntial virus, yellow fever virus et al. Complications from cardiovascular system can come to light at various age.Статья посвящена изучению представленных эпидемиологических, клинических, инструментальных, лабораторных данных о патологии сердечно-сосудистой системы при различных вирусных инфекциях. Обзор основан на результатах отечественных и зарубежных исследований. При вирусных инфекциях поражение сердца и его проводящей системы возможно как в острый период болезни, так и в периоде реконвалесценции или при хроническом течении вирусного процесса. Наибольшей кардиотропностью обладают энтеровирусы, которые поражают миокард в 5–15% случаев. Большое внимание уделяется герпес-вирусам, широко распространенным, длительно персистирующим в организме, как одной из причин развития дилатационной кардиомиопатии, коронарного васкулиста, раннего атеросклероза, нарушения ритма сердца. К другим инфекциям, при которых возможно поражение сердечно-сосудистой системы, следует отнести вирусы гриппа, аденовирус, вирус полиомиелита, вирус Эпштейна – Барр, цитомегаловирус, вирус иммунодефицита человека, вирусы гепатита, паротита, краснухи, простого герпеса, ветряной оспы, арбовирус, респираторно-синтициальный вирус, вирус желтой лихорадки и др. Осложнения и функциональные нарушения со стороны сердечно-сосудистой системы могут выявляться вне зависимости от возраста

Glutamyl endopeptidase of Bacillus intermedius, strain 3-19

A homogeneous glutamyl endopeptidase splitting peptide bonds of glutamic, rarely of aspartic acid residues in peptides and proteins, was isolated from Bacillus intermedius 3-19 culture filtrate using chromatography on CM cellulose and Mono S. The enzyme molecular mass is equal to 29 kDa, pI 8.4. The protease is inhibited by diisopropylfluorophosphate. The enzyme, like other glutamyl endopeptidases, reveals two pH optima at pH 7.5 and 9.0 for casein and one at pH 8.0 for Z-Glu-pNA hydrolysis. A 6 mM K(m) is found for hydrolysis of the latter substrate. The enzyme activity optimum lies at 55°C, and it is stable at pH 6.5-11.0. Its N-terminal sequence shows 56% coinciding residues when compared with that of Bacillus licheniformis glutamyl endopeptidase