Alternative pathways of the initial transformation of 2,4,6-trinitrotoluene by yeasts

A new model for the initial transformation of 2,4,6-trinitrotoluene (TNT) by facultatively anaerobic and aerobic yeasts is presented. The model is based on the data that Saccharomyces sp. ZS-Al was able to reduce the nitrogroups of TNT with the formation of 2- and 4-hydroxyaminodinitrotoluenes (2-HADNT and 4-HADNT) as the major early TNT metabolites (the molar HADNT/TNT ratio reached 0.81), whereas aminodinitrotoluenes (ADNTs) and the hydride-Meisenheimer complex of TNT (H-TNT) were the minor products. Candida sp. AN-L13 almost completely transformed TNT into H-TNT through the reduction of the aromatic ring. Candida sp. AN-L14 transformed TNT through a combination of the two mechanisms described. Aeration stimulated the production of HADNT from TNT, whereas yeast incubation under stationary conditions promoted the formation of HADNT. The transformation of TNT into HADNT led to a tenfold increase in the acute toxicity of the TNT preparation with respect to Paramecium caudatum, whereas the increase in the toxicity was about twofold in the case of the alternative attack at the aromatic ring

High-voltage pulse discharge as factor of the methanogenesis initiation

Results of scientific research on influence of the high-voltage pulse discharge on process of bioorganic waste methane sludge fermentation in agricultural production are given in the article. Powerful infra- and ultrasonic fluctuations leading to emergence of shock waves exert strong impact on processes: disinfecting, cleanings and deflocculation of bioorganic mix, as well as on activity of various physical and chemical changes of synthesis products. Selecting the modes of high-voltage pulse processing, it is possible to provide highly productive anaerobic bacteria. At the same time "artificial selection" as a result of which weak microorganisms perish is observed, and the strong group remains. Viable species of microorganisms, having received at the order nutrient medium, as a result of destruction and death of weak microorganisms, begin to breed quickly, increasing growth of a biofilm responsible for a biogas exit. For the analysis of experimental data on development of technological process for anaerobic sludge fermentation the method of trans-resonant functional topography which provided informational content of the active environment concerning ions and free radicals arising in the course of modifying the organic substratum was used.Keywords: biogas unit, anaerobic sludge fermentation, electro technology, methanogenesis,pulse discharge, bioorganic waste, water substratum, organic fertilizers, processing, ecology

Bryophyte Diversity of Calcareous Fens in the Bashkir Cis-Urals (Republic of Bashkortostan, the Southern Urals)

The bryophyte diversity of base-rich fens was studied in 16 calcareous mires of the Bashkir Cis-Urals (the Southern Ural region). Thirty-seven moss species and nine liverworts were recorded in treeless communities dominated by Schoenus ferrugineus, Molinia caerulea, small sedges and mosses. The annotated species list with precise locations is provided. Most of the surveyed mires are located in the northeastern part of the Bashkir Cis-Urals, that is, in the Mesyagutovo forest-steppe bordering with the western foothills of the Southern Ural Mountains. In the plain areas of the western part of the Bashkir Cis-Urals, calcareous mires are very rare and characterized by the low number of habitat specialists. Arctic-boreo-alpine species (Cinclidium stygium,Paludella squarrosa, Palustriella decipiens, Pseudocalliergon trifarium, etc.) make up approximately 30% of the total bryophyte diversity of the surveyed fens. In the study area, these species grow in small isolated populations at the southern limit of the species range. Currently, only half of the surveyed calcareous mires are located within protected areas. It is essential to improve the protection of these unique habitats inthe study area. Keywords: calcareous mires, base-rich fens, bryophytes, the Southern Ural

Food allergy to nuts and fruits in children with hay fever and oral allergy syndrome in the middle Urals

The aim of research is to study the frequency of true sensitization to nuts and fruits in children sensitized to Bet v 1 and having oral allergy syndrome (OAS) and hay fever

Alternative pathways of the initial transformation of 2,4,6-trinitrotoluene by yeasts

A new model for the initial transformation of 2,4,6-trinitrotoluene (TNT) by facultatively anaerobic and aerobic yeasts is presented. The model is based on the data that Saccharomyces sp. ZS-Al was able to reduce the nitrogroups of TNT with the formation of 2- and 4-hydroxyaminodinitrotoluenes (2-HADNT and 4-HADNT) as the major early TNT metabolites (the molar HADNT/TNT ratio reached 0.81), whereas aminodinitrotoluenes (ADNTs) and the hydride-Meisenheimer complex of TNT (H-TNT) were the minor products. Candida sp. AN-L13 almost completely transformed TNT into H-TNT through the reduction of the aromatic ring. Candida sp. AN-L14 transformed TNT through a combination of the two mechanisms described. Aeration stimulated the production of HADNT from TNT, whereas yeast incubation under stationary conditions promoted the formation of HADNT. The transformation of TNT into HADNT led to a tenfold increase in the acute toxicity of the TNT preparation with respect to Paramecium caudatum, whereas the increase in the toxicity was about twofold in the case of the alternative attack at the aromatic ring. © 2002 MAIK "Nauka/Interperiodica"

Three Dimensional Relativistic Electromagnetic Sub-cycle Solitons

Three dimensional (3D) relativistic electromagnetic sub-cycle solitons were observed in 3D Particle-in-Cell simulations of an intense short laser pulse propagation in an underdense plasma. Their structure resembles that of an oscillating electric dipole with a poloidal electric field and a toroidal magnetic field that oscillate in-phase with the electron density with frequency below the Langmuir frequency. On the ion time scale the soliton undergoes a Coulomb explosion of its core, resulting in ion acceleration, and then evolves into a slowly expanding quasi-neutral cavity.Comment: 5 pages, 6 figures; http://www.ile.osaka-u.ac.jp/research/TSI/Timur/soliton/index.htm

Transformation of 2,4,6-trinitrotoluene into toxic hydroxylamino derivatives by Lactobacilli

Lactobacilli isolated from different ecological niches were capable of partial nitroreduction of 2,4,6-trinitrotoluene (TNT) to hydroxylaminodinitrotoluenes (HADNT) at a high rate (up to 9.3 nmol/(min mg dry biomass)). For the most active (with respect to the reaction rate) strains, Lactobacillus fermentum BS3601 and Lactobacillus plantarum BS3604, the extent of transformation comprised 95-97%. An inverse correlation was found between the ability to transform TNT and the resistance of bacteria to its toxic action. The inhibitory effects of TNT and HADNT on the activities of glucose-6-phosphate dehydrogenase (G-6-PDH) and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) in cell extracts of lactobacilli were revealed. © 1999 MAHK "Hayka/Interperiodica"

Initial stages of 2,4,6-trinitrotoluene transformation by microorganisms

Screening of a wide range of microorganisms (32 strains) isolated from various anthropogenic and natural environments and of a number of collection strains showed that the early stages of 2,4,6-trinitrotoluene (TNT) transformation by the majority of the strains studied resulted in the formation of hydroxylaminodinitrotoluenes (HADNTs). The levels of HADNTs were in a number of cases comparable to the initial TNT level. The alternative reductive attack on TNT through the reduction of the aromatic ring was not characteristic of most of the prokaryotes studied. The susceptibility to the toxic effect of TNT was different for gram-positive and gram-negative bacteria

Initial stages of 2,4,6-trinitrotoluene transformation by microorganisms

Screening of a wide range of microorganisms (32 strains) isolated from various anthropogenic and natural environments and of a number of collection strains showed that the early stages of 2,4,6-trinitrotoluene (TNT) transformation by the majority of the strains studied resulfed in the formation of hydroxylaminodinitrotoluenes (HADNTs). The levels of HADNTs were in a number of cases comparable to the initial TNT level. The alternative reductive attack at TNT through the reduction of the aromatic ring was not characteristic of most of the prokaryotes studied. The susceptibility to the toxic effect of TNT was different for gram-positive and gram-negative bacteria