Defect Characterization by Spatial Distribution of Ultrasonic Scattered Energy

The ultrasonic pulse echo technique is a highly sensitive nondestructive method for detecting small defects within the bulk of a structure. The technique is capable of reliably finding small volumetric defects when the defect size is approximately 0.030 inches in diameter and the specimen thicknesses are not too great. If the distance between the transducer and the defect is large, greater than 2 near field distances, the minimum value of volumetric flaw sizes that can be reliably detected rises considerably. For 6-8 inch thick plates, it is not too surprising to occasionally miss volumetric defects such as slag inclusions of considerable length

Gonadal hormones, but not sex, affect the acquisition and maintenance of a Go/No-Go odor discrimination task in mice

In mice, olfaction is crucial for identifying social odors (pheromones) that signal the presence of suitable mates. We used a custom-built olfactometer and a thirst-motivated olfactory discrimination Go/No-Go (GNG) task to ask whether discrimination of volatile odors is sexually dimorphic and modulated in mice by adult sex hormones. Males and females gonadectomized prior to training failed to learn even the initial phase of the task, which involved nose poking at a port in one location obtaining water at an adjacent port. Gonadally intact males and females readily learned to seek water when male urine (S+) was present but not when female urine (S−) was present; they also learned the task when non-social odorants (amyl acetate, S+; peppermint, S−) were used. When mice were gonadectomized after training the ability of both sexes to discriminate urinary as well as non-social odors was reduced; however, after receiving testosterone propionate (castrated males) or estradiol benzoate (ovariectomized females), task performance was restored to pre-gonadectomy levels. There were no overall sex differences in performance across gonadal conditions in tests with either set of odors; however, ovariectomized females performed more poorly than castrated males in tests with non-social odors. Our results show that circulating sex hormones enable mice of both sexes to learn a GNG task and that gonadectomy reduces, while hormone replacement restores, their ability to discriminate between odors irrespective of the saliency of the odors used. Thus, gonadal hormones were essential for both learning and maintenance of task performance across sex and odor type.We thank David Giese for help in programming the apparatus used in GNG testing and Alberto Cruz-Martin for comments on an early version of the manuscript. This work was supported by NIDCD grant DC008962 to JAC. (DC008962 - NIDCD grant)Accepted manuscrip

How Do Bacteria Move Through Soil?

The contamination of water supplies by fecal bacteria is an important water quality issue in Kentucky. Contamination may come from point sources, such as straight pipes depositing raw sewage into streams, or nonpoint sources, such as manure runoff from cropland. A direct cost of contaminating water supplies is the expense that homesteads or water companies incur to chlorinate, filter, and otherwise treat water to make it potable. Indirect costs are the time lost to illness from drinking inadequately treated water, slower weight gain in livestock drinking contaminated water, and the degradation of aquatic habitats

Empathy Gaps Between Helpers and Help-Seekers: Implications for Cooperation

Help-seekers and potential helpers often experience an “empathy gap” – an inability to understand each other’s unique perspectives. Both parties are concerned about their reputation, self-esteem, and relationships, but these concerns differ in ways that lead to misinterpretation of the other party’s actions, and, in turn, missed opportunities for cooperation. In this article, we review research that describes the role-specific concerns of helpers and help-seekers. We then review studies of emotional perspective-taking, which can help explain why help-seekers and helpers often experience empathy gaps. We go on to discuss recent work that illustrates the consequences of empathy gaps between helpers and help-seekers—social prediction errors that prevent helping and misguided intentions that can lead to unhelpful help. Finally, we discuss some promising directions for future research

Morphological and taxonomic studies of Gracilaria and Gracilariopsis species (Gracilariales, Rhodophyta) from South Africa

Southern Africa has an extremely rich and diverse seaweed flora with a wide variety of marine habitats. Increased commercial interest in these seaweed resources has been the stimulus for biodiversity studies. The Gracilariaceae (Rhodophyta) has emerged as one of the families that possess economic potential as a source of agar and as a potential feed for abalone. A lack of knowledge concerning the taxonomic status of many members of this family is a concern. Gross morphological characters have been the main means of identification and incorrect applications have led to a number of misidentifications. Consequently, a comprehensive reappraisal and revision of these species was carried out. The species count for the South African Gracilariaceae is now two Gracilariopsis species, and nine Gracilaria species. Gracilaria crassa has been reduced to a synonym of G. canaliculata. It is believed that G. foliifera was erroneously identified and specimens in South Africa referred to as G. millardetii and G. protea are assigned to G. corticata. South African Gracilariopsis, previously referred to as Gs. lemaneiformis, is confirmed to be conspecific with European Gs. longissima. This species occurs along the west and south coasts of South Africa, co-existing in a few habitats with G. gracilis. The taxonomic identity of G. vieillardii specimens from South Africa and the differentiation of G. canaliculata and G. salicornia has been confirmed based on morphology. Three species of Gracilaria (G. aculeata, G. beckeri and G. capensis) are endemic or near endemic to the South African coast, and a fourth species, G. denticulata is localised in southeast Africa

Nematode distribution and damage to yam in central and eastern Uganda

Yams (Dioscorea spp.) are food crops of growing significance in sub Saharan Africa. Unfortunately, nematodes are major pests to their production. A study was undertaken in major yam growing areas of Uganda to investigate the association of plant parasitic nematodes with damage symptoms. Nematodes were assessed from tubers, roots and surrounding soil for seven cultivars belonging to Dioscorea alata, D. bulbisiana, D. burkilliana and D. cayenensis at harvest. Pratylenchus sudanensis was found in the greatest density and was followed byMeloidogyne spp. Higher densities of both nematodes were observed in the tubers rather than roots. Although symptoms of cracking and galling were relatively low, P. sudanensis incidence was strongly associated with cracking and Meloidogyne spp. with galling. Pratylenchus sudanensis and Meloidogyne spp. incidence their associated damage were, however, negatively correlated, suggesting inter-species competition. Principal components analysis of data suggested that differences in susceptibility to P. sudanensis occur between yam cultivars and species

Notes on Recent Cases

Notes on recent cases by William J. Coyne, Henry Hasley, Albert T. Franz, E. F. McClarnon, Marc Wonderlin, Thomas V. Happer, Edward P. McGuire, John P. Berscheid, and T. J. Jones, Jr

Developing social capital in implementing a complex intervention: a process evaluation of the early implementation of a suicide prevention intervention in four European countries

<p>Background: Variation in the implementation of complex multilevel interventions can impact on their delivery and outcomes. Few suicide prevention interventions, especially multilevel interventions, have included evaluation of both the process of implementation as well as outcomes. Such evaluation is essential for the replication of interventions, for interpreting and understanding outcomes, and for improving implementation science. This paper reports on a process evaluation of the early implementation stage of an optimised suicide prevention programme (OSPI-Europe) implemented in four European countries.</p> <p>Methods: The process analysis was conducted within the framework of a realist evaluation methodology, and involved case studies of the process of implementation in four European countries. Datasets include: repeated questionnaires to track progress of implementation including delivery of individual activities and their intensity; serial interviews and focus groups with stakeholder groups; and detailed observations at OSPI implementation team meetings.</p> <p>Results: Analysis of local contexts in each of the four countries revealed that the advisory group was a key mechanism that had a substantial impact on the ease of implementation of OSPI interventions, particularly on their ability to recruit to training interventions. However, simply recruiting representatives of key organisations into an advisory group is not sufficient to achieve impact on the delivery of interventions. In order to maximise the potential of high level ‘gatekeepers’, it is necessary to first transform them into OSPI stakeholders. Motivations for OSPI participation as a stakeholder included: personal affinity with the shared goals and target groups within OSPI; the complementary and participatory nature of OSPI that adds value to pre-existing suicide prevention initiatives; and reciprocal reward for participants through access to the extended network capacity that organisations could accrue for themselves and their organisations from participation in OSPI.</p> <p>Conclusions: Exploring the role of advisory groups and the meaning of participation for these participants revealed some key areas for best practice in implementation: careful planning of the composition of the advisory group to access target groups; the importance of establishing common goals; the importance of acknowledging and complementing existing experience and activity; and facilitating an equivalence of benefit from network participation.</p&gt

Evidence of Extensive DNA Transfer between Bacteroidales Species within the Human Gut

ABSTRACT The genome sequences of intestinal Bacteroidales strains reveal evidence of extensive horizontal gene transfer. In vitro studies of Bacteroides and other bacteria have addressed mechanisms of conjugative transfer and some phenotypic outcomes of these DNA acquisitions in the recipient, such as the acquisition of antibiotic resistance. However, few studies have addressed the horizontal transfer of genetic elements between bacterial species coresident in natural microbial communities, especially microbial ecosystems of humans. Here, we examine the genomes of Bacteroidales species from two human adults to identify genetic elements that were likely transferred among these Bacteroidales while they were coresident in the intestine. Using seven coresident Bacteroidales species from one individual and eight from another, we identified five large chromosomal regions, each present in a minimum of three of the coresident strains at near 100% DNA identity. These five regions are not found in any other sequenced Bacteroidetes genome at this level of identity and are likely all integrative conjugative elements (ICEs). Such highly similar and unique regions occur in only 0.4% of phylogenetically representative mock communities, providing strong evidence that these five regions were transferred between coresident strains in these subjects. In addition to the requisite proteins necessary for transfer, these elements encode proteins predicted to increase fitness, including orphan DNA methylases that may alter gene expression, fimbriae synthesis proteins that may facilitate attachment and the utilization of new substrates, putative secreted antimicrobial molecules, and a predicted type VI secretion system (T6SS), which may confer a competitive ecological advantage to these strains in their complex microbial ecosystem