The Evolution of Distorted Rotating Black Holes III: Initial Data

In this paper we study a new family of black hole initial data sets corresponding to distorted ``Kerr'' black holes with moderate rotation parameters, and distorted Schwarzschild black holes with even- and odd-parity radiation. These data sets build on the earlier rotating black holes of Bowen and York and the distorted Brill wave plus black hole data sets. We describe the construction of this large family of rotating black holes. We present a systematic study of important properties of these data sets, such as the size and shape of their apparent horizons, and the maximum amount of radiation that can leave the system during evolution. These data sets should be a very useful starting point for studying the evolution of highly dynamical black holes and can easily be extended to 3D.Comment: 16 page

Trapped Surfaces in Vacuum Spacetimes

An earlier construction by the authors of sequences of globally regular, asymptotically flat initial data for the Einstein vacuum equations containing trapped surfaces for large values of the parameter is extended, from the time symmetric case considered previously, to the case of maximal slices. The resulting theorem shows rigorously that there exists a large class of initial configurations for non-time symmetric pure gravitational waves satisfying the assumptions of the Penrose singularity theorem and so must have a singularity to the future.Comment: 14 page

Cosmological expansion on a dilatonic brane-world

In this paper we study brane-world scenarios with a bulk scalar field, using a covariant formalism to obtain a 4D Einstein equation via projection onto the brane. We discuss, in detail, the effects of the bulk on the brane and how the scalar field contribute to the gravitational effects. We also discuss choice of conformal frame and show that the frame selected by the induced metric provides a natural choice. We demonstrate our formalism by applying it to cosmological scenarios of Randall-Sundrum and Horava-Witten type models. Finally we consider the cosmology of models where the scalar field couples non-minimally to the matter on the brane. This gives rise to a novel scenario where the universe expands from a finite scale factor with an initial period of accelerated expansion, thus avoiding the singularity and flatness problem of the standard big bang model.Comment: 20 pages - Version to appear in Classical and Quantum Gravity. New section added on conformal rescaling of the metric. Some other minor changes made and references adde

Identification and characterization of genes encoding sex pheromone cAM373 activity in Enterococcus faecalis and Staphylococcus aureus

The sex pheromone cAM373 of Enterococcus faecalis and the related staph -cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably serve unrelated functions in the cells. The chromosomally encoded genetic determinants for production of the pheromones have been identified and designated camE (encoding cAM373) and camS (encoding staph -cAM373). Truncated and full-length clones of camE were generated in Escherichia coli , in which cAM373 activity was expressed. In E. faecalis , insertional inactivation in the middle of camE had no detectable phenotypic effects on the pheromone system. Establishment of an in frame translation stop codon within the signal sequence resulted in reduction of cAM373 activity to 3% of normal levels. The camS determinant has homologues in Staphylococcus epidermidis , Bacillus subtilis and Listeria monocytogenes ; however, corresponding heptapeptides present within those sequences do not resemble staph -cAM373 closely. The particular significance of staph -cAM373 as a potential intergeneric inducer of transfer-proficient genetic elements is discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75605/1/j.1365-2958.2002.02922.x.pd

Coxiella burnetii Phagocytosis Is Regulated by GTPases of the Rho Family and the RhoA Effectors mDia1 and ROCK

The GTPases belonging to the Rho family control the actin cytoskeleton rearrangements needed for particle internalization during phagocytosis. ROCK and mDia1 are downstream effectors of RhoA, a GTPase involved in that process. Coxiella burnetii, the etiologic agent of Q fever, is internalized by the host´s cells in an actin-dependent manner. Nevertheless, the molecular mechanism involved in this process has been poorly characterized. This work analyzes the role of different GTPases of the Rho family and some downstream effectors in the internalization of C. burnetii by phagocytic and non-phagocytic cells. The internalization of C. burnetii into HeLa and RAW cells was significantly inhibited when the cells were treated with Clostridium difficile Toxin B which irreversibly inactivates members of the Rho family. In addition, the internalization was reduced in HeLa cells that overexpressed the dominant negative mutants of RhoA, Rac1 or Cdc42 or that were knocked down for the Rho GTPases. The pharmacological inhibition or the knocking down of ROCK diminished bacterium internalization. Moreover, C. burnetii was less efficiently internalized in HeLa cells overexpressing mDia1-N1, a dominant negative mutant of mDia1, while the overexpression of the constitutively active mutant mDia1-ΔN3 increased bacteria uptake. Interestingly, when HeLa and RAW cells were infected, RhoA, Rac1 and mDia1 were recruited to membrane cell fractions. Our results suggest that the GTPases of the Rho family play an important role in C. burnetii phagocytosis in both HeLa and RAW cells. Additionally, we present evidence that ROCK and mDia1, which are downstream effectors of RhoA, are involved in that processFil: Salinas Ojeda, Romina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Ortiz Flores, Rodolfo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Distel, Jesús Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Aguilera, Milton Osmar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Beron, Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentin

Gravitational radiation from gamma-ray bursts as observational opportunities for LIGO and VIRGO

Gamma-ray bursts are believed to originate in core-collapse of massive stars. This produces an active nucleus containing a rapidly rotating Kerr black hole surrounded by a uniformly magnetized torus represented by two counter-oriented current rings. We quantify black hole spin-interactions with the torus and charged particles along open magnetic flux-tubes subtended by the event horizon. A major output of Egw=4e53 erg is radiated in gravitational waves of frequency fgw=500 Hz by a quadrupole mass-moment in the torus. Consistent with GRB-SNe, we find (i) Ts=90s (tens of s, Kouveliotou et al. 1993), (ii) aspherical SNe of kinetic energy Esn=2e51 erg (2e51 erg in SN1998bw, Hoeflich et al. 1999) and (iii) GRB-energies Egamma=2e50 erg (3e50erg in Frail et al. 2001). GRB-SNe occur perhaps about once a year within D=100Mpc. Correlating LIGO/Virgo detectors enables searches for nearby events and their spectral closure density 6e-9 around 250Hz in the stochastic background radiation in gravitational waves. At current sensitivity, LIGO-Hanford may place an upper bound around 150MSolar in GRB030329. Detection of Egw thus provides a method for identifying Kerr black holes by calorimetry.Comment: to appear in PRD, 49

Driver Behavior as a Function of Ambient Light and Road Geometry

OBJECTIVES To determine how ambient light (day versus night) and road geometry affect driving behavior,especially the speeds that drivers choose when not constrained by lead vehicles.METHODSRecently, it has become technically easier to observe how people drive b offering them longtermuse of highly instrumented vehicles. Much of this type of work has been done in connectionwith large-scale field operational tests (FOTs) of various innovative vehicle systems. Theinformation obtained is in many ways complementary to information from observation of traffic.Traffic observation often provides information about a large number of drivers, but at a relativelycoarse level and in a spatially and temporally limited context (i.e., observing how a large numberof drivers negotiate a particular intersection). In contrast, long-term use of highly instrumentedvehicles is more restricted in terms of how many drivers can be observed, although the feasiblenumbers are now reasonably high. On the positive side, data from instrumented vehicles canoffer very detailed information about driving behavior over many miles and many days.In this paper, we present results from a database of driving behavior that was derived from arecent FOT for an adaptive cruise control (ACC) system (although the data used here are all fromphases of the study that involved only normal vehicle equipment). The FOT involved tenidentical cars that were instrumented for a variety of types of data. The most important data forpresent purposes were: speed, yaw rate, location from the Global Positioning System (GPS), andpresence or absence of a lead vehicle within about 100 m based on the forward-looking sensorsof the ACC system. The instrumented cars were driven by a total of 108 participants, each ofwhom was given a car to use as his or her own vehicle in normal driving for either two or fiveweeks. The participants were sampled from licensed drivers in southeastern Michigan, andrepresented a wide range of age and driving experience.RESULTSResults will be reported in terms of speed as a function of horizontal road curvature in light anddark conditions, and as a function of driver age and gender, all for situations in which there is nolead vehicle within about 100 m. CONCLUSIONSCurrent evidence about headlighting suggests that drivers’ ability to see and negotiate theroadway is virtually unaffected by differences in ambient light, although their ability to perceiveand avoid objects on the road, such as pedestrians, is greatly reduced when headlamps are themain source of light. There is also evidence that drivers do not markedly reduce their speed inconditions of low ambient light. The current analysis allows us to determine how drivers react tospecific road geometries in light and dark conditions. This has implications for how well drivers’perceptual abilities match their driving behavior, and also for assessing the potential benefit of avariety of innovative headlighting systems that are currently being designed to adapt in variousways to vehicle speed and road geometry

Driver Behavior as a Function of Ambient Light and Road Geometry

OBJECTIVES To determine how ambient light (day versus night) and road geometry affect driving behavior,especially the speeds that drivers choose when not constrained by lead vehicles.METHODSRecently, it has become technically easier to observe how people drive b offering them longtermuse of highly instrumented vehicles. Much of this type of work has been done in connectionwith large-scale field operational tests (FOTs) of various innovative vehicle systems. Theinformation obtained is in many ways complementary to information from observation of traffic.Traffic observation often provides information about a large number of drivers, but at a relativelycoarse level and in a spatially and temporally limited context (i.e., observing how a large numberof drivers negotiate a particular intersection). In contrast, long-term use of highly instrumentedvehicles is more restricted in terms of how many drivers can be observed, although the feasiblenumbers are now reasonably high. On the positive side, data from instrumented vehicles canoffer very detailed information about driving behavior over many miles and many days.In this paper, we present results from a database of driving behavior that was derived from arecent FOT for an adaptive cruise control (ACC) system (although the data used here are all fromphases of the study that involved only normal vehicle equipment). The FOT involved tenidentical cars that were instrumented for a variety of types of data. The most important data forpresent purposes were: speed, yaw rate, location from the Global Positioning System (GPS), andpresence or absence of a lead vehicle within about 100 m based on the forward-looking sensorsof the ACC system. The instrumented cars were driven by a total of 108 participants, each ofwhom was given a car to use as his or her own vehicle in normal driving for either two or fiveweeks. The participants were sampled from licensed drivers in southeastern Michigan, andrepresented a wide range of age and driving experience.RESULTSResults will be reported in terms of speed as a function of horizontal road curvature in light anddark conditions, and as a function of driver age and gender, all for situations in which there is nolead vehicle within about 100 m. CONCLUSIONSCurrent evidence about headlighting suggests that drivers’ ability to see and negotiate theroadway is virtually unaffected by differences in ambient light, although their ability to perceiveand avoid objects on the road, such as pedestrians, is greatly reduced when headlamps are themain source of light. There is also evidence that drivers do not markedly reduce their speed inconditions of low ambient light. The current analysis allows us to determine how drivers react tospecific road geometries in light and dark conditions. This has implications for how well drivers’perceptual abilities match their driving behavior, and also for assessing the potential benefit of avariety of innovative headlighting systems that are currently being designed to adapt in variousways to vehicle speed and road geometry

A Fluorescence Based-Proliferation Assay for the Identification of Replicating Bacteria Within Host Cells

Understanding host pathogen interactions is paramount to the development of novel antimicrobials. An important facet of this pursuit is the accurate characterization of pathogen replication within infected host cells. Here we describe the use of a fluorescence-based proliferation assay to identify intracellular populations of replicating bacteria at the subcellular level. Using Staphylococcus aureus as a model Gram-positive bacterial pathogen and macrophages as a model host phagocyte, we demonstrate this assay can be used to reliably identify individual phagocytes that contain replicating bacteria. Furthermore, we demonstrate this assay is compatible with additional cellular probes that enable characterization of cellular compartments in which replicating bacteria reside. Finally, we demonstrate that this assay facilitates the investigation of both Gram-negative and Gram-positive bacteria within host cells

Dynamic macrophage “probing” is required for the efficient capture of phagocytic targets

Rather than passively binding ligands via immunoreceptors, macrophages capture particles by repeated extension of actin-rich protrusions