Электромагнитные поля компактных люминесцентных энергосберегающих ламп

The questions related to potential environmental and hygienic hazard, which may be caused by electromagnetic field of compact energy saving lamps, are considered. The results of measurements of electromagnetic fields in low frequency domain: spectral composition, intensity depending on distance for lamps of various types and power consumption are presented. It is shown that maximum value of electromagnetic field frequency of 50 Hz from the measured energy saving lamps does not exceed the maximum permissible levels.Рассматриваются вопросы, связанные с возможной эколого-гигиенической опасностью, которые могут представлять электромагнитные поля компактных энергосберегающих ламп. Приведенырезультаты измерений электромагнитных полей в низкочастотной области: спектрального состава, напряженности в зависимости от расстояния для ламп различных видов и потребляемой мощности. Показано, что максимальные величины напряженности электромагнитного поля частотой 50 Гц от измеренных энергосберегающих ламп не превышают предельно допустимых уровней

Proteomic Signature of Extracellular Vesicles for Lung Cancer Recognition

The proteins of extracellular vesicles (EVs) that originate from tumors reflect the producer cells’ proteomes and can be detected in biological fluids. Thus, EVs provide proteomic signatures that are of great interest for screening and predictive cancer diagnostics. By applying targeted mass spectrometry with stable isotope-labeled peptide standards, we assessed the levels of 28 EV-associated proteins, including the conventional exosome markers CD9, CD63, CD81, CD82, and HSPA8, in vesicles derived from the lung cancer cell lines NCI-H23 and A549. Furthermore, we evaluated the detectability of these proteins and their abundance in plasma samples from 34 lung cancer patients and 23 healthy volunteers. The abundance of TLN1, TUBA4A, HSPA8, ITGB3, TSG101, and PACSIN2 in the plasma of lung cancer patients was measured using targeted mass spectrometry and compared to that in plasma from healthy volunteers. The most diagnostically potent markers were TLN1 (AUC, 0.95), TUBA4A (AUC, 0.91), and HSPA8 (AUC, 0.88). The obtained EV proteomic signature allowed us to distinguish between the lung adenocarcinoma and squamous cell carcinoma histological types. The proteomic cargo of the extracellular vesicles represents a promising source of potential biomarkers

PROGNOSTIC VALUE OF GLUT-1 EXPRESSION IN THE HIGHLY DIFFERENTIATED THYROID CANCER

OBJECTIVE. Analysis of GLUT1 levels as a prognostic marker for highly differentiated thyroid carcinoma.MATERIAL AND METHODS. The expression levels of GLUT-1, NIS, thyroglobulin and the presence of BRAF mutation were analyzed in 32 patients with highly differentiated thyroid cancer from the Department of Surgical Endocrinology at the Research Institute for Surgery and Emergency Medicine of “Pavlov First Saint Petersburg State Medical University”.RESULTS. There was a trend to increased levels of GLUT-1 in patients with regional or intra-organ metastases or peripheral invasion. This suggests that increased expression of GLUT-1 is found in proliferatively active cells. In our study, the expression level of GLUT-1 was independent of the level of NIS, thyroglobulin and the presence of BRAF mutation. Thus, the level of expression of GLUT-1 can be considered as an independent factor of the prognosis of the course of highly differentiated thyroid carcinoma.CONCLUSION. The level of expression of GLUT-1 could potentially be used as a prognostic marker in highly differentiated thyroid cancer

The role of defining the sentinel lymph node in the treatment of low-risk highly differentiated thyroid cancer (review of literature)

The definition of a sentinel lymph node is a technique that appeared at the middle of the 20th century. For a number of malignant neoplasms, the definition of a sentinel lymph node is standard procedure, but for highly differentiated thyroid cancer, the data is conflicting. The role of prophylactic central compartment lymph node dissection in the clinical N0 stage is not established. Sentinel lymph node detection seems to be the possible way to reduce the risk of persistence and recurrence of highly differentiated thyroid cancer

200+Protein Concentrations in Healthy Human Blood Plasma: Targeted Quantitative SRM SIS Screening of Chromosomes 18, 13, Y, and the Mitochondrial Chromosome Encoded Proteome

Mitochondria are undeniably the cell powerhouse, directly affecting cell survival and fate. Growing evidence suggest that mitochondrial protein repertoire affects metabolic activity and plays an important role in determining cell proliferation/differentiation or quiescence shift. Consequently, the bioenergetic status of a cell is associated with the quality and abundance of the mitochondrial populations and proteomes. Mitochondrial morphology changes in the development of different cellular functions associated with metabolic switches. It is therefore reasonable to speculate that different cell lines do contain different mitochondrialassociated proteins, and the investigation of these pools may well represent a source for mining missing proteins (MPs). A very effective approach to increase the number of IDs through mass spectrometry consists of reducing the complexity of the biological samples by fractionation. The present study aims at investigating the mitochondrial proteome of five phenotypically different cell lines, possibly expressing some of the MPs, through an enrichment 12fractionation approach at the organelle and protein level. We demonstrate a substantial increase in the proteome coverage, which, in turn, increases the likelihood of detecting low abundant proteins, often falling in the category of MPs, and resulting, for the present study, in the identification of METTL12, FAM163A, and RGS13. All MS data have been deposited to the MassIVE data repository (https://massive.ucsd. edu) with the data set identifier MSV000082409 and PXD010446