IMPROVEMENT OF KNOWLEDGE OF STUDENTS OF ELEMENTARY SCHOOL OF RANGKAH KIDUL ON HAND WASH THROUGH HEALTH PROMOTION

ABSTRACT Health Promotion Activities with the title Clean and Healthy Lifestyle with Hand Washing at SDN Rangkah Kidul Sidoarjo Sidoarjo District Sidoarjo Regency is one form of community service in the form of counseling aimed at growing and improving healthy behavior in children from an early age, especially in PHBS Hand Washing. The implementation of these activities on November 15, 2018 took place at Elementary School of Rangkah Kidul, Sidoarjo District, Sidoarjo Regency. The target is students of Elementary School of Rangkah Kidul, Sidoarjo District, Sidoarjo Regency. Before the activity is carried out, there is a process of compiling the activity for 3 weeks before the activity is carried out, starting from the selection of health counseling materials to the submission of licensing to the relevant parties. As an evaluation, the activity was attended by 50 students and 2 teachers, participants participated in the activity with enthusiasm and conducive, the activity can be carried out on time smoothly. Keywords: Hand washing, health promotion, elementary school, counseling

Role of Maltose Enzymes in Glycogen Synthesis by Escherichia coli▿

Mutants with deletion mutations in the glg and mal gene clusters of Escherichia coli MC4100 were used to gain insight into glycogen and maltodextrin metabolism. Glycogen content, molecular mass, and branch chain distribution were analyzed in the wild type and in ΔmalP (encoding maltodextrin phosphorylase), ΔmalQ (encoding amylomaltase), ΔglgA (encoding glycogen synthase), and ΔglgA ΔmalP derivatives. The wild type showed increasing amounts of glycogen when grown on glucose, maltose, or maltodextrin. When strains were grown on maltose, the glycogen content was 20 times higher in the ΔmalP strain (0.97 mg/mg protein) than in the wild type (0.05 mg/mg protein). When strains were grown on glucose, the ΔmalP strain and the wild type had similar glycogen contents (0.04 mg/mg and 0.03 mg/mg protein, respectively). The ΔmalQ mutant did not grow on maltose but showed wild-type amounts of glycogen when grown on glucose, demonstrating the exclusive function of GlgA for glycogen synthesis in the absence of maltose metabolism. No glycogen was found in the ΔglgA and ΔglgA ΔmalP strains grown on glucose, but substantial amounts (0.18 and 1.0 mg/mg protein, respectively) were found when they were grown on maltodextrin. This demonstrates that the action of MalQ on maltose or maltodextrin can lead to the formation of glycogen and that MalP controls (inhibits) this pathway. In vitro, MalQ in the presence of GlgB (a branching enzyme) was able to form glycogen from maltose or linear maltodextrins. We propose a model of maltodextrin utilization for the formation of glycogen in the absence of glycogen synthase