Heteroptera (Insecta) fauna of Iranian cotton fields and surrounding grasslands

The Heteroptera fauna (Insecta) of Iranian cotton fields and surrounding areas is studied. A total of 103 species from 14 families were found. Among the collected material, 7 species are new records for Iranian fauna: Cryptostemma (Pachycoleus) gracile Josifov 1967, Cryptostemma (Pachycoleus) utnapishtim Linnavuori 1984, Systelloderes utukhengal Linnavuori 1984, Phytocoris (Eckerleinius) paghmanus Linnavuori 1974, Himacerus (Himacerus) apterus (Fabricius 1798), Nabis (Dolichonabis) limbatus Dahlbom 1851 and Reduvius osiris Kirkaldy 1909.La faune des Hétéroptères (Insecta) de champs de coton iraniens est étudiée. Un total de 103 espèces appartenant à 14 familles fut collecté. Parmi le matériel récolté, 7 espèces sont nouvelles pour la faune d'Iran: Cryptostemma (Pachycoleus) gracile Josifov 1967, Cryptostemma (Pachycoleus) utnapishtim Linnavuori 1984, Systelloderes utukhengal Linnavuori 1984, Phytocoris (Eckerleinius) paghmanus Linnavuori 1974, Himacerus (Himacerus) apterus (Fabricius 1798), Nabis (Dolichonabis) limbatus Dahlbom 1851 et Reduvius osiris Kirkaldy 1909.Fil: Ghahari, Hassan. Islamic Azad University; IránFil: Chérot, Frédéric. Free University of Brussels; BélgicaFil: Moulet, Pierre. Museum Requien; FranciaFil: Carpintero, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; ArgentinaFil: Linnavuori, Rauno E.. Saukkokuja; FinlandiaFil: Sakenin, Hamid. Islamic Azad University; IránFil: Ostovan, Hadi. Islamic Azad University; Irá

Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?

Exposure to environmental and occupational particulate matter (PM) induces health effects on the cardio-pulmonary system. In addition, associations between exposure to PM and metabolic syndromes like diabetes mellitus or obesity are now emerging in the literature. Collection of exhaled breath condensate (EBC) is an appealing non-invasive technique to sample pulmonary fluids. This hypothesis-generating study aims to (1) validate an ion chromatography method allowing the robust determination of different metabolism-related molecules (lactate, formate, acetate, propionate, butyrate, pyruvate, nitrite, nitrate) in EBC; (2) apply this method to EBC samples collected from workers exposed to quartz (a known inflammatory particle), to soapstone (a less inflammatory particle than quartz), as well as to controls. A multi-compound standard solution was used to determine the linearity range, detection limit, repeatability and bias from spiked EBC. The biological samples were injected without further treatment into an ion chromatograph with a conductivity detector. RTube <sup>®</sup> were used for field collection of EBC from 11 controls, 55 workers exposed to soapstone and 12 volunteers exposed to quartz dust. The analytical method used proved to be adequate for quantifying eight anions in EBC samples. Its sub-micromolar detection limits and repeatability, combined with a very simple sample preparation, allowed an easy and fast quantification of different glycolysis or nitrosative stress metabolites. Using multivariate discriminant analysis to maximize differences between groups, we observed a different pattern of anions with a higher formate/acetate ratio in the EBC samples for quartz exposed workers compared to the two other groups. We hypothesize that a modification of the metabolic signature could be induced by exposure to inflammatory particles like quartz and might be observed in the EBC via a change in the formate/acetate ratio

Analysis of nitrogen oxides (NOx) in the exhaled breath condensate (EBC) of subjects with asthma as a complement to exhaled nitric oxide (FeNO) measurements: a cross-sectional study

<p>Abstract</p> <p>Background</p> <p>The study of pulmonary biomarkers with noninvasive methods, such as the analysis of exhaled breath condensate (EBC), provides a useful approach to the pathophysiology of asthma. Although many recent publications have applied such methods, numerous methodological pitfalls remain. The first stage of our study consisted of validating methods for the collection, storage and analysis of EBC; we next sought to clarify the utility of analysing nitrogen oxides (NOx) in the EBC of asthmatics, as a complement to measuring exhaled nitric oxide (FeNO).</p> <p>Methods</p> <p>This hospital-based cross-sectional study included 23 controls matched with 23 asthmatics. EBC and FeNO were performed and respiratory function measured. Intra-assay and intra-subject reproducibility were assessed for the analysis of NOx in the EBC of 10 healthy subjects.</p> <p>Results</p> <p>The intraclass correlation coefficient (ICC) was excellent for intra-assay reproducibility and was moderate for intra-subject reproducibility (Fermanian's classification). NOx was significantly higher in asthmatics (geometric mean [IQR] 14.4 μM [10.4 - 19.7] vs controls 9.9 μM [7.5 - 15.0]), as was FeNO (29.9 ppb [17.9 - 52.4] vs controls 9.6 ppb [8.4 - 14.2]). FeNO also increased significantly with asthma severity.</p> <p>Conclusions</p> <p>We validated the procedures for NOx analysis in EBC and confirmed the need for assays of other biomarkers to further our knowledge of the pathophysiologic processes of asthma and improve its treatment and control.</p

伊朗西北部阿拉斯巴兰及附近的蝽类

A list of Heteroptera collected from Arasbaran Biosphere Reserve (East Azarbayjan Province, northwestern Iran) and neighboring areas is provided. A total of 114 species from 87 genera and 19 families were collected from Arasbaran and vicinity. Two species are new records for Iran: Gerris (Gerris) maculatus Tamanini and Sigara (Vermicorixa) lateralis (Leach).Fil: Ghahari, Hassan. Islamic Azad University; IránFil: Cai, Wan-zhi. China Agricultural University; ChinaFil: Chérot, Frédéric. Free University of Brussels; BélgicaFil: Carpintero, Diego Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; ArgentinaFil: Moulet, Pierre. Museum Requien; FranciaFil: Ostovan, Hadi. Islamic Azad University; IránFil: Montemayor Borsinger, Sara Itzel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Entomología; ArgentinaFil: Havaskary, Mohammad. Islamic Azad University; IránFil: Linnavuori, Rauno E.. Saukkokuja; Finlandi

Using medical exome sequencing to identify the causes of neurodevelopmental disorders: Experience of 2 clinical units and 216 patients

International audienceAlthough whole-exome sequencing (WES) is the gold standard for the diagnosis of neurodevelopmental disorders (NDDs), it remains expensive for some genetic centers. Commercialized panels comprising all OMIM-referenced genes called "medical exome" (ME) constitute an alternative strategy to WES, but its efficiency is poorly known. In this study, we report the experience of 2 clinical genetic centers using ME for diagnosis of NDDs. We recruited 216 consecutive index patients with NDDs in 2 French genetic centers, corresponded to the daily practice of the units and included non-syndromic intellectual disability (NSID, n = 33), syndromic ID (NSID = 122), pediatric neurodegenerative disorders (n = 7) and autism spectrum disorder (ASD, n = 54). We sequenced samples from probands and their parents (when available) with the Illumina TruSight One sequencing kit. We found pathogenic or likely pathogenic variants in 56 index patients, for a global diagnostic yield of 25.9%. The diagnosis yield was higher in patients with ID as the main diagnosis (32%) than in patients with ASD (3.7%). Our results suggest that the use of ME is a valuable strategy for patients with ID when WES cannot be used as a routine diagnosis tool