National competitiveness as the object of indicative planning in the context of re-industrialization

Three years after the adoption of legislation regarding strategical planning we are able to see the first statistical results, reflecting actual conditions of regulated objects. The subject of this article is to examine the system of indicators of state economic policy, targeting to stimulate the development of economic potential with the aim to improve national economic competitiveness. The objective is the competitiveness of domestic economy as the subject of regulation. The aim is the analysis of the system of indicators on the basis of comparison of actual and planned data, detecting weakly correlated indicators and to make recommendations to correct the system of indicators. The article analyses the indicators that are being used in normative and legal documents. The authors consider long-term and medium-term targets for economic frontier development, as well as main factors that should be accounted while formulating the system of indicators for the economic potential in long- and medium-term. As the list of indicators of economic development in the strategic documents is vast, the chosen direction considers the production with high added value. The indicators considered reflect the indicative aim of scientific development, national innovation system and technology as the most important foundations for improvement of national economic competitiveness. Multidirectional trend of economic development and administration indicators has been detected. The formation of system of supplementary indicators that reflect the development of production frontier has been proposed.peer-reviewe

CIVILIZATIONAL IDENTITY OF RUSSIAN STUDENT YOUTH IN A DIGITAL SOCIETY

The modern concept of civilization, processes of civilizational identification and identity on the basis of allocation of basic values have been considered. Traditional spiritual and moral values of Russian population have been studied. Methodological and methodical issues of sociological diagnostics of civilizational identity of Russian students have been revealed. Some results of the author’s sociological research with presentation of a comparative assessment of the orientation of young people on the values of the Russian civilization have been adduced. On the basis of comparative analysis, the description of social constructs (patterns) of values of the Russian civilization has been given, the data on the statistical connection of the civilizational identity of students and their integration into virtual forms of social interaction have been presented. The measures to improve the conditions of the process of civilizational identification of Russian students and to include them in the process of digitalization of society have been substantiated

Genetic environment of the blaKPC-2 gene in a Klebsiella pneumoniae isolate that may have been imported to Russia from Southeast Asia

The nucleotide sequence of a blaKPC-2-harboring plasmid (pKPCAPSS) from Klebsiella pneumoniae ST273 isolated in Saint Petersburg, Russia, from a patient with history of recent travel to Vietnam is presented. This 127,970-bp plasmid possessed both IncFII and IncR replicons. blaKPC-2 was localized on a hypothetical mobile element. This element was flanked by 38-bp inverted Tn3 repeats and included a Tn3-specific transposase gene, macrolide resistance operon (mphA-mrx-mphR), and a fragment of blaTEM with unique polymorphisms. © 2017 American Society for Microbiology. All Rights Reserved

A positive experience in applying the biolistic approach to potato varieties Aksor and Nevskiy

The method of biological ballistics (biolistic transformation, genetic bombardment) of plants is one of the most modern methods used for direct gene transfer into plant cells. The main advantages of this method include the ability to simultaneously incorporate several target genes into the plant genome, carry out transfer without unnecessary agrobacterial parts and plasmid DNA sequences, and the short time needed to produce transgenic cells. For different plant objects, the efficiency of obtaining transgenic plants by the ballistic method varies from 1 to 3 %. For potato plants, the transformation efficiency is quite low at the moment and the selection of optimal conditions for biolistics is one of the pressing issues of practical biotechnology. This article presents a successful experience of introducing two genes of interest into two potato varieties using the biolistic approach. The results of biolistic transformation experiments are presented for two types of explants: potato internodes and calli of the varieties Aksor and Nevskiy. Of the 862 explants used for transformation, 56 regenerated plants were obtained. PCR screening of transformants revealed one plant with the insertion of the chitinase gene, one with the insertion of the endo-β-1,3-glucanase gene, and co-transformation by both genes was confirmed in four regenerants. The average transformation efficiency for potato explants was 0.7 %. A high number of regenerants (56) as opposed to a low number of transformants (6) reflects an attempt to increase the number of regenerants by using a lower concentration of the selective agent (kanamycin). Although this approach requires more effort, it can be used to produce potato lines with integrated genes of interest for further use in crop breeding. The lines of potato obtained in the current study by introducing two genes associated with the plant response to fungal pathogens will be further assessed for their resistance to fungal diseases and, if successful, will be used in potato crop breeding

О ВЫПОЛНЕНИИ КООРДИНАЦИОННЫХ НАУЧНО-ТЕХНИЧЕСКИХ ПРОГРАММ ПО ВЕ­ТЕРИНАРНОЙ ПАРАЗИТОЛОГИИ

Fundamental and applied studies on the regularities of formation and functioning of parasitic systems and their components in natural and agricultural biocenoses of different ecological and climatic zones in Russian Federation and some CIS countries were carried out.  Monitoring and forecast of epizootic situation on parasitic diseases in farm animals and wildlife was conducted. Methods for prevention and fight against parasitoses were elaborated. Search for the next generation of diagnostic tools and complex remedies for protection of animals was conducted.Проведены фундаментальные и прикладные исследования по изучению закономерностей формирования и функционирования паразитарных систем и их компонентов в условиях естественных и сельскохозяйствен­ных биоценозов в различных эколого-климатических регионах на территории Российской Федерации и неко­торых стран СНГ, по мониторингу и прогнозированию эпизоотической ситуации по паразитарным болезням с.х. и диких животных и разработке методов борьбы и профилактики паразитозов, поиску нового поколения диагностических и комплексных средств защиты животных

Data on gut metagenomes of the patients with Helicobacter pylori infection before and after the antibiotic therapy

© 2017Antibiotic therapy can lead to the disruption of gut microbiota community with possible negative outcomes for human health. One of the diseases for which the treatment scheme commonly included antibiotic intake is Helicobacter pylori infection. The changes in taxonomic and functional composition of microbiota in patients can be assessed using “shotgun” metagenomic sequencing. Ten stool samples were collected from 4 patients with Helicobacter pylori infection before and directly after the H. pylori eradication course. Additionally, for two of the subjects, the samples were collected 1 month after the end of the treatment. The samples were subject to “shotgun” (whole-genome) metagenomic sequencing using Illumina HiSeq platform. The reads are deposited in the ENA (project ID: PRJEB18265)

Герпес-вирусы 6 и 7 типа: взгляд педиатра

Herpetic diseases are widespread among cancer patients and are of interest to specialists in various fields. It is herpetic infections that are often common among cases of infant mortality. The main causative agents of herpetic phenomena in humans are manifested by the 8 most studied types of the virus. The article presents a review of modern scientific literature on herpetic diseases caused by herpes viruses 6 and 7. The issues of the history of the study of diseases, etiology, features of the distribution, the pathogenesis of diseases, a wide manifestation of manifestations in childhood, diagnosis, and tactics of managing patients are outlined.Герпетические инфекции широко распространены в человеческой популяции, и интерес к ним среди специалистов разного профиля постоянно возрастает, так как они являются причиной развития большого спектра соматических и онкологических заболеваний. Именно герпетические инфекции часто являются причинами преждевременных родов, младенческой смертности. Основные возбудители герпетических инфекций у человека представлены 8 наиболее изученными типами вируса. В статье представлен обзор современной научной литературы по вопросам герпетических инфекций, вызванных герпес-вирусами 6-го и 7-го типа. Изложены вопросы истории изучения заболевания, этиология, особенности распространения, патогенез заболевания, основные клинические проявления в детском возрасте, диагностика и тактика ведения пациентов

In vitro maturation for fertility preservation in patients with cancer: A review

According to the World Health Organization, the number of patients of reproductive age with cancer steadily increases. Therefore, improving their quality of life is one of the priority tasks of the medical community. It is known that more than 30% of women with cancer at the time of diagnosis have not yet given birth. Therefore, one of the relevant issues is developing and improving methods for preserving reproductive function. In vitro oocyte maturation is a promising technique of oncofertility, which is used as an alternative to traditional cycles of ovulation stimulation followed by the production of mature oocytes. This review aims to study the in vitro maturation procedure and analyze the literature data regarding its effectiveness and safety when used as a part of programs for preserving reproductive material in patients with cancer

Search for Specific Biomarkers of IFNβ Bioactivity in Patients with Multiple Sclerosis

Myxovirus A (MxA), a protein encoded by the MX1 gene with antiviral activity, has proven to be a sensitive measure of IFNβ bioactivity in multiple sclerosis (MS). However, the use of MxA as a biomarker of IFNβ bioactivity has been criticized for the lack of evidence of its role on disease pathogenesis and the clinical response to IFNβ. Here, we aimed to identify specific biomarkers of IFNβ bioactivity in order to compare their gene expression induction by type I IFNs with the MxA, and to investigate their potential role in MS pathogenesis. Gene expression microarrays were performed in PBMC from MS patients who developed neutralizing antibodies (NAB) to IFNβ at 12 and/or 24 months of treatment and patients who remained NAB negative. Nine genes followed patterns in gene expression over time similar to the MX1, which was considered the gold standard gene, and were selected for further experiments: IFI6, IFI27, IFI44L, IFIT1, HERC5, LY6E, RSAD2, SIGLEC1, and USP18. In vitro experiments in PBMC from healthy controls revealed specific induction of selected biomarkers by IFNβ but not IFNγ, and several markers, in particular USP18 and HERC5, were shown to be significantly induced at lower IFNβ concentrations and more selective than the MX1 as biomarkers of IFNβ bioactivity. In addition, USP18 expression was deficient in MS patients compared with healthy controls (p = 0.0004). We propose specific biomarkers that may be considered in addition to the MxA to evaluate IFNβ bioactivity, and to further explore their implication in MS pathogenesis