Electrochemical Activity of Glutathione at a Graphite Electrode Modified with Gold Nanoparticles

The electrochemical activity of the glutathione (GSH) at the graphite electrodes modified with gold nanoparticles (AuNP-GCE) is formed under the ratio of reactants and in solutions of supporting electrolytes of 0, 1 M NaOH, 0,1 “ phosphate buffer (pH 6.86), 0,1 M HCl, 0,1 M NaClO4 is studied by the method of cyclic voltammetry. The dependence of the electrochemical activity of GSH on ratio of reagents HAuCl4 :Na3 C6 H5 O7 :NaBH4 , used to the preparation of sols, and the modification time of the GE surface with gold nanoparticles have been studied. The “inverse” cathodic peak of GSH at AuNPGCE formed in the reaction mixture HAuCl4 :Na3 C6 H5 O7 :NaBH4 = 1:1:4 is observed on the cathodic branch of cyclic voltammogram at Ec = 0.05 V in 0.1 M NaOH. The mechanism of electrochemical oxidation of the GSH on the surface of AuNP-GCE in 0.1 M NaOH is proposed

Environmental monitoring of natural waters in Krasnodar and Stavropol Territories

The environmental monitoring of natural waters in Krasnodar (Uspensky and Novokubansky districts) and Stavropol (Kochubeyevsky District) Territories was conducted. In the course of study, various elements and compounds harmful to animals and humans, which exceed maximum permissible concentrations, were identified

Blood parameters in dermatitis of various etiologies in cattle

The purpose of the research is to identify a general pattern in the change in hematological and biochemical parameters in any lesions of cattle skin.Materials and methods. The work was carried out in October-November 2021 in the conditions of farms in the Ipatovsky district of the Stavropol Territory on cattle aged 9–12 months and 90–120 kg of body weight. During the veterinary clinical examination, four groups of fve heads each were formed, one of the groups was a control. Animals of the control group had no clinical signs of dermatitis. The experimental groups of animals were divided depending on the degree of intensity of skin lesions into mild, moderate and generalized forms of manifestation of dermatitis. The results obtained were processed statistically.Results and discussion. A veterinary clinical examination of this livestock made it possible to establish the dependence in case of damage to the skin not only in changes in temperature, respiratory rate and pulse, but also in blood parameters. The body temperature of calves with a generalized form of dermatitis increased by an average of 0.5 degrees, breathing increased by six units per minute, the pulse rate increased by 15–18 beats per minute. In calves with mild and moderate manifestation of dermatitis, these parameters did not have signifcant changes and were within the normal range. From hematological indicators, hemoglobin had a general tendency to decrease with increasing intensity of the lesion or aggravation of the pathological process. Such indicators as ESR, leukocytes, neutrophils, eosinophils, on the contrary, tended to increase, especially in the generalized form. The slowdown of erythropoiesis and thrombocytopenia in the generalized form of dermatitis is presumably associated with intoxication of the body of calves. An increase in ESR, the number of eosinophils indicates an inflammatory process occurring in the animal's body. From biochemical indicators, an increase in the level of bilirubin, AST and ALT, creatinine, alkaline phosphatase indicates toxic liver damage due to intoxication of the body of calves due to inflammatory factors and decay products of affected skin cells

Показатели крови при дерматитах различной этиологии у крупного рогатого скота

The purpose of the research is to identify a general pattern in the change in hematological and biochemical parameters in any lesions of cattle skin.Materials and methods. The work was carried out in October-November 2021 in the conditions of farms in the Ipatovsky district of the Stavropol Territory on cattle aged 9–12 months and 90–120 kg of body weight. During the veterinary clinical examination, four groups of fve heads each were formed, one of the groups was a control. Animals of the control group had no clinical signs of dermatitis. The experimental groups of animals were divided depending on the degree of intensity of skin lesions into mild, moderate and generalized forms of manifestation of dermatitis. The results obtained were processed statistically.Results and discussion. A veterinary clinical examination of this livestock made it possible to establish the dependence in case of damage to the skin not only in changes in temperature, respiratory rate and pulse, but also in blood parameters. The body temperature of calves with a generalized form of dermatitis increased by an average of 0.5 degrees, breathing increased by six units per minute, the pulse rate increased by 15–18 beats per minute. In calves with mild and moderate manifestation of dermatitis, these parameters did not have signifcant changes and were within the normal range. From hematological indicators, hemoglobin had a general tendency to decrease with increasing intensity of the lesion or aggravation of the pathological process. Such indicators as ESR, leukocytes, neutrophils, eosinophils, on the contrary, tended to increase, especially in the generalized form. The slowdown of erythropoiesis and thrombocytopenia in the generalized form of dermatitis is presumably associated with intoxication of the body of calves. An increase in ESR, the number of eosinophils indicates an inflammatory process occurring in the animal's body. From biochemical indicators, an increase in the level of bilirubin, AST and ALT, creatinine, alkaline phosphatase indicates toxic liver damage due to intoxication of the body of calves due to inflammatory factors and decay products of affected skin cells.Цель исследований – выявление общей закономерности в изменении гематологических и биохимических показателей при любых поражениях кожного покрова крупного рогатого скота.Материалы и методы. Работа проведена в октября-ноябре 2021 г. в условиях хозяйств Ипатовского района Ставропольского края на крупном рогатом скоте в возрасте 9–12 мес. массой тела 90–120 кг. В ходе ветеринарно-клинического обследования были сформированы четыре группы по пять голов в каждой, одна из групп была контрольной. Животные контрольной группы не имели клинических признаков дерматитов. Опытные группы животных были разделены в зависимости степени интенсивности поражения кожи на слабую, среднюю и генерализованную формы проявления дерматитов. Полученные результаты обработаны статистически.Результаты и обсуждение. Ветеринарно-клинический осмотр данного поголовья позволил установить зависимость при поражении кожного покрова не только в изменениях показателей температуры, частоты дыхания и пульса, но и показателей крови. Температура тела у телят с генерализованной формой дерматита повышалась в среднем на 0,5 градуса, дыхание учащалось на шесть единиц в минуту, частота пульса возрастала на 15–18 ударов в минуту. У телят со слабым и средним проявлением дерматита данные показатели не имели значимых изменений и находились в пределах нормы. Из гематологических показателей, гемоглобин имел общую тенденцию к снижению с ростом интенсивности поражения или усугубления патологического процесса. Такие показатели как СОЭ, лейкоциты, нейтрофилы, эозинофилы, наоборот, имели тенденцию к увеличению, особенно при генерализованной форме. Замедление эритропоэза и тромбоцитопении при генерализованной форме дерматита предположительно связаны с интоксикацией организма телят. Повышение СОЭ, числа эозинофилов свидетельствует о воспалительном процессе, происходящем в организме животного. Из биохимических показателей, повышение уровня билирубина, АСТ и АЛТ, креатинина, щелочной фосфатазы свидетельствует о токсическом поражении печени вследствие интоксикации организма телят за счет воспалительных факторов и продуктов распада пораженных клеток кожи

Comparative analysis of lactaptin activity when produced in bacterial or eukaryotic expression systems

Despite the multitude of anticancer cytostatic drugs available to oncologists today, most of such drugs have serious side effects that may preclude their use in some groups of patients. Hence, selective induction of apoptosis in cancer but not normal cells remains an attractive goal of molecular medicine. Lactaptin, a proteolytic fragment of the human milk kappa-casein, has been previously identified as a protein displaying potent killing of cancer cells in vitro. Its recombinant analog (RL2) produced in E. coli has been shown to delay solid tumor growth in vivo. Given that lactaptin is of human origin and is not immunogenic, it can be administered to patients multiple times without running the risk of immune response that could dampen the therapy efficacy. In the present study, we demonstrate that the combination of RL2 and cyclophosphamide treatments has an additive therapeutic effect against hepatoma tumor in immunocompetent mice. We asked whether production of lactaptin in human rather than bacterial cells would result in a protein with increased cytotoxic activity. Using lentiviral vector pCDH as a backbone, two constructs, pEL1 and pEL2, encoding secreted forms of lactaptin that differ in their signal sequences were created. Lactaptin expression in human cell lines was confirmed using Western-blot analysis, whereas ELISA was used for quantification of secreted lactaptin. Next, we measured the cytotoxic effects of the media conditioned by pEL1-transfected HEK293T cells, as assayed against the panel of three human cancer cell lines: MDA-MB-231 (adenocarcinoma), PC3 (prostate cancer), and T98G (glioblastoma). We show that EL1-derived lactaptin is at least 100-fold more cytotoxic than RL2. Taken together, our results provide an opportunity for developing armored immune cells as an “off-the-shelf” platform for targeted delivery of lactaptin to cancer cells

Общие принципы профилактики и лечения осложнений чрескожной эндоскопической гастростомы (обзор литературы)

We considered the views of researchers presented in the modern literature on both the problem as a whole and discussion questions regarding the causes of development, preventive measures, and methods of treating percutaneous endoscopic gastrostomy complications, such as clogging of the gastrostomy tube, peristomal wound infections, necrotic fasciitis, pneumoperitoneum, buried bumper syndrome, growth of granulations in the gastrostomy zone, postoperative bleeding and intraparietal hematoma of the gastric wall, traumatic dislocation of the gastrostomy tube, peritonitis after percutaneous endoscopic gastrostomy, peristomal leakage, сolonic fistula, liver injury and abdominal wall metastasis at the percutaneous endoscopic gastrostomy site.Приведен обзор современных методов профилактики и лечения осложнений, возникающих после формирования чрескожной эндоскопической гастростомы. Рассмотрены представленные в современной литературе взгляды исследователей как на проблему в целом, так и на дискуссионные вопросы, касающиеся причин развития, мер профилактики и способов лечения осложнений чрескожной эндоскопической гастростомы, таких как засорение гастростомической трубки, нагноение послеоперационной раны, некротический фасциит, пневмоперитонеум, бампер-синдром, рост грануляций в зоне гастростомы, послеоперационное кровотечение и внутристеночная гематома стенки желудка, травматическая дислокация гастростомической трубки, перитонит после чрескожной эндоскопической гастростомы, перистомальная несостоятельность, толстокишечный свищ, ранение печени и имплантационный метастаз в канале чрескожной эндоскопической гастростомы. Необходимо дальнейшее изучение данной проблемы с проведением рандомизированных контролируемых клинических исследований

First πK\pi K atom lifetime and πK\pi K scattering length measurements

The results of a search for hydrogen-like atoms consisting of $\pi^{\mp}K^{\pm}$ mesons are presented. Evidence for $\pi K$ atom production by 24 GeV/c protons from CERN PS interacting with a nickel target has been seen in terms of characteristic $\pi K$ pairs from their breakup in the same target ($178 \pm 49$) and from Coulomb final state interaction ($653 \pm 42$). Using these results the analysis yields a first value for the $\pi K$ atom lifetime of $\tau=(2.5_{-1.8}^{+3.0})$ fs and a first model-independent measurement of the S-wave isospin-odd $\pi K$ scattering length $\left|a_0^-\right|=\frac{1}{3}\left|a_{1/2}-a_{3/2}\right|= \left(0.11_{-0.04}^{+0.09} \right)M_{\pi}^{-1}$ ($a_I$ for isospin $I$).Comment: 14 pages, 8 figure

Comprehensive analysis of the chromatin landscape in Drosophila melanogaster.

Chromatin is composed of DNA and a variety of modified histones and non-histone proteins, which have an impact on cell differentiation, gene regulation and other key cellular processes. Here we present a genome-wide chromatin landscape for Drosophila melanogaster based on eighteen histone modifications, summarized by nine prevalent combinatorial patterns. Integrative analysis with other data (non-histone chromatin proteins, DNase I hypersensitivity, GRO-Seq reads produced by engaged polymerase, short/long RNA products) reveals discrete characteristics of chromosomes, genes, regulatory elements and other functional domains. We find that active genes display distinct chromatin signatures that are correlated with disparate gene lengths, exon patterns, regulatory functions and genomic contexts. We also demonstrate a diversity of signatures among Polycomb targets that include a subset with paused polymerase. This systematic profiling and integrative analysis of chromatin signatures provides insights into how genomic elements are regulated, and will serve as a resource for future experimental investigations of genome structure and function

Гематологические показатели крупного рогатого скота при паразитарных дерматитах

The purpose of the research is study of the animal's body clinical status and changes in hematological parameters before and after treatment against sarcoptoidosis of cattle. Materials and methods. The experiment was performed on bull calves and heifers aged 6–10 months, medium finish, of 100–120 kg of body weight. The test animals were divided into three groups: two test groups and one control group of 15 animals each. Before the experiment and at 7, 14 and 21 days after treatment with acaricides, scrapings from the affected skin and blood were taken from animals of all groups for research. The first test group animals were treated with ivermectin subcutaneously at a dose of 5 ml per animal, and the second test group was treated with cydectin subcutaneously at a dose of 5 ml per animal. The control animals were not treated. Results and discussion. The conducted hematological studies found a decrease in erythrocytes and hemoglobin in sarcoptoidosis, which indicated a decrease in oxygen supply to the body, and, consequently, organs and tissues. The white blood cell differential was observed to have a noticeable decrease in monocytes and lymphocytes.Цель исследований: изучение клинического статуса организма животного, изменений гематологических показателей до применения и после лечения при саркоптоидозах крупного рогатого скота.Материалы и методы. Опыт проводили на бычках и телочках в возрасте 6–10 мес., средней упитанности, живой массой 100–120 кг. Подопытных животных разделили на три группы: две опытные и одну контрольную по 15 голов в каждой. До опыта и через 7, 14 и 21 сут после обработки акарицидными препаратами у животных всех групп брали соскобы с пораженных участков кожного покрова и кровь для исследования. Животные первой опытной группы были обработаны ивермектином подкожно в область предплечья в дозе из расчета 5 мл на животное, а второй опытной группы – цидектином подкожно в дозе из расчета 5 мл на животное в область предплечья. Животных контрольной группы не обрабатывали. Результаты и обсуждение. В процессе проведенных гематологических исследований было установлено снижение количества эритроцитов и гемоглобина при саркоптоидозах, что свидетельствовало о снижении поступления кислорода в организм, а, следовательно, и в органы и ткани. В лейкоцитарной формуле наблюдали заметное снижение числа моноцитов и лимфоцитов

New data on OZI rule violation in bar{p}p annihilation at rest

The results of a measurement of the ratio R = Y(phi pi+ pi-) / Y(omega pi+ pi-) for antiproton annihilation at rest in a gaseous and in a liquid hydrogen target are presented. It was found that the value of this ratio increases with the decreasing of the dipion mass, which demonstrates the difference in the phi and omega production mechanisms. An indication on the momentum transfer dependence of the apparent OZI rule violation for phi production from the 3S1 initial state was found.Comment: 11 pages, 3 PostScript figures, submitted to Physics Letter