The Charging Structure for the Great Barrier Reef - A review of willingness to pay

The Great Barrier Reef Marine Park Authority (GBRMPA or the Reef Authority) is currently conducting a comprehensive review of the charging structure for the use of the Great Barrier Reef (GBR) Marine Park with a view to implement potential changes from 1 July 2023

In-situ measurement of journal bearing lubricant viscosity by means of a novel ultrasonic measurement technique using matching layer

An ultrasonic viscometer was used to measure the circumferential viscosity variation in a journal bearing non-invasively. This sensing technique is based on the reflection of a shear wave at a solid-liquid boundary that depends on the viscosity of the liquid and the acoustic properties of the solid. Very little ultrasonic energy can propagate into the oil at a metal-oil interface because the acoustic mismatch is significant. Interleaving a matching layer between the metal and the lubricant enables accurate ultrasonic viscosity measurements [1] Schirru, M., Mills, R., Dwyer-Joyce, R., Smith, O., and Sutton, M. (2015). Viscosity Measurement in a Lubricant Film Using an Ultrasonically Resonating Matching Layer. Tribology Letters, 60(3) pp. 1–11. [CrossRef], [Web of Science ®] . This technique has been used to build a miniaturized ultrasonic viscometer that is accommodated inside a journal to obtain the circumferential viscosity profile. Four viscosity regions are identified due to the variations in the localized temperatures and loads. The results are compared with the isothermal solution of the Reynolds equations for hydrodynamic lubricated bearings. The ultrasonic viscometer locates the angle at which the maximum load occurs and the length of the loaded contact with good accuracy. Finally, the viscosity results are used to estimate the frictional power losses. It is shown that over 70% of the total losses in the journal bearing occur in the region where the load is maximum

Space Debris Detection in Low Earth Orbit with the Sardinia Radio Telescope

Space debris are orbiting objects that represent a major threat for space operations. The most used countermeasure to face this threat is, by far, collision avoidance, namely the set of maneuvers that allow to avoid a collision with the space debris. Since collision avoidance is tightly related to the knowledge of the debris state (position and speed), the observation of the orbital debris is the key of the problem. In this work a bistatic radar configuration named BIRALET (BIstatic RAdar for LEO Tracking) is used to detect a set of space debris at 410 MHz, using the Sardinia Radio Telescope as the receiver antenna. The signal-to-noise ratio, the Doppler shift and the frequency spectrum for each debris are reported

Toward the renal vesicle: Ultrastructural investigation of the cap mesenchyme splitting process in the developing kidney

Background: A complex sequence of morphogenetic events leads to the development of the adult mouse kidney. In the present study, we investigated the morphological events that characterize the early stages of the mesenchymal-to-epithelial transition of cap mesenchymal cells, analyzing in depth the relationship between cap mesenchymal induction and ureteric bud (UB) branching. Design and methods: Normal kidneys of newborn non-obese diabetic (NOD) mice were excised and prepared for light and electron microscopic examination. Results: Nephrogenesis was evident in the outer portion of the renal cortex of all examined samples. This process was mainly due to the interaction of two primordial derivatives, the ureteric bud and the metanephric mesenchyme. Early renal developmental stages were initially characterized by the formation of a continuous layer of condensed mesenchymal cells around the tips of the ureteric buds. These caps of mesenchymal cells affected the epithelial cells of the underlying ureteric bud, possibly inducing their growth and branching. Conclusions: The present study provides morphological evidence of the reciprocal induction between the ureteric bud and the metanephric mesenchyme showing that the ureteric buds convert mesenchyme to epithelium that in turn stimulates the growth and the branching of the ureteric bud

Inter-Laboratory Concordance of Cerebrospinal Fluid and Serum Kappa Free Light Chain Measurements

The kappa index (K-Index), calculated by dividing the cerebrospinal fluid (CSF)/serum kappa free light chain (KFLC) ratio by the CSF/serum albumin ratio, is gaining increasing interest as a marker of intrathecal immunoglobulin synthesis. However, data on inter-laboratory agreement of these measures is lacking. The aim was to assess the concordance of CSF and serum KFLC measurements, and of K-index values, across different laboratories. KFLC and albumin of 15 paired CSF and serum samples were analyzed by eight participating laboratories. Four centers used Binding Site instruments and assays (B), three used Siemens instruments and assays (S), and one center used a Siemens instrument with a Binding Site assay (mixed). Absolute individual agreement was calculated using a two-way mixed effects intraclass correlation coefficient (ICC). Cohen’s kappa coefficient (k) was used to measure agreement on positive (5.8) K-index values. There was an excellent agreement in CSF KFLC measurements across all laboratories (ICC (95% confidence interval): 0.93 (0.87–0.97)) and of serum KFLC across B and S laboratories (ICC: 0.91 (0.73–0.97)), while ICC decreased (to 0.81 (0.53–0.93)) when including the mixed laboratory in the analysis. Concordance for a positive K-Index was substantial across all laboratories (k = 0.77) and within S laboratories (k = 0.71), and very good (k = 0.89) within B laboratories, meaning that patients rarely get discordant results on K-index positivity notwithstanding the testing in different laboratories and the use of different platforms/assays

Vitamin D responsive elements within the HLA-DRB1 promoter region in Sardinian multiple sclerosis associated alleles

Vitamin D response elements (VDREs) have been found in the promoter region of the MS-associated allele HLA-DRB1*15:01, suggesting that with low vitamin D availability VDREs are incapable of inducing *15:01 expression allowing in early life autoreactive T-cells to escape central thymic deletion. The Italian island of Sardinia exhibits a very high frequency of MS and high solar radiation exposure. We test the contribution of VDREs analysing the promoter region of the MS-associated DRB1 *04:05, *03:01, *13:01 and *15:01 and non-MS-associated *16:01, *01, *11, *07:01 alleles in a cohort of Sardinians (44 MS patients and 112 healthy subjects). Sequencing of the DRB1 promoter region revealed a homozygous canonical VDRE in all *15:01, *16:01, *11 and in 45/73 *03:01 and in heterozygous state in 28/73 *03:01 and all *01 alleles. A new mutated homozygous VDRE was found in all *13:03, *04:05 and *07:01 alleles. Functionality of mutated and canonical VDREs was assessed for its potential to modulate levels of DRB1 gene expression using an in vitro transactivation assay after stimulation with active vitamin D metabolite. Vitamin D failed to increase promoter activity of the *04:05 and *03:01 alleles carrying the new mutated VDRE, while the *16:01 and *03:01 alleles carrying the canonical VDRE sequence showed significantly increased transcriptional activity. The ability of VDR to bind the mutant VDRE in the DRB1 promoter was evaluated by EMSA. Efficient binding of VDR to the VDRE sequence found in the *16:01 and in the *15:01 allele reduced electrophoretic mobility when either an anti-VDR or an anti-RXR monoclonal antibody was added. Conversely, the Sardinian mutated VDRE sample showed very low affinity for the RXR/VDR heterodimer. These data seem to exclude a role of VDREs in the promoter region of the DRB1 gene in susceptibility to MS carried by DRB1* alleles in Sardinian patients

Graphene-based ultra-wide band printed bow-tie antenna for remote tracking

Printed antennas are cheap, lightweight, easy to- fabricate with high precision, and adaptable to mass production. These features are desirable for both indoor and outdoor handheld UWB antenna applications. In this paper, we consider planar versions of a high performance ultra-wide band bow-tie antenna with omnidirectional radiation characteristics

Observing galaxy clusters and the cosmic web through the Sunyaev Zel'dovich effect with MISTRAL

Galaxy clusters and surrounding medium, can be studied using X-ray bremsstrahlung emission and Sunyaev Zel'dovich (SZ) effect. Both astrophysical probes, sample the same environment with different parameters dependance. The SZ effect is relatively more sensitive in low density environments and thus is useful to study the filamentary structures of the cosmic web. In addition, observations of the matter distribution require high angular resolution in order to be able to map the matter distribution within and around galaxy clusters. MISTRAL is a camera working at 90GHz which, once coupled to the Sardinia Radio Telescope, can reach $12''$ angular resolution over $4'$ field of view (f.o.v.). The forecasted sensitivity is $NEFD \simeq 10-15mJy \sqrt{s}$ and the mapping speed is $MS= 380'^{2}/mJy^{2}/h$. MISTRAL was recently installed at the focus of the SRT and soon will take its first photons.Comment: To appear in Proc. of the mm Universe 2023 conference, Grenoble (France), June 2023, published by F. Mayet et al. (Eds), EPJ Web of conferences, EDP Science

Improving access to and reuse of research results, publications and data for scientific purposes – Study to evaluate the effects of the EU copyright framework on research and the effects of potential interventions and to identify and present relevant provisions for research in EU data and digital legislation, with a focus on rights and obligations (Publications Office of the European Union)

This report supports ERA Policy Agenda 2022-2024, aiming at an EU framework for copyright and data fit for research. It analyses barriers to accessing and reusing publicly funded research, evaluating EU copyright and data legislation, along with regulatory frameworks. Presented measures aim to enhance the current framework, aligning it with scientific research and open data principles. It offers a comprehensive overview of the EU's research and innovation legal landscape, providing insights for policymakers, researchers, and research organisations