Influence of the twilight period and different sampling methods on catch of Gobiids (Gobiidae) at four locations in the inshore parts of the Danube River

The aim of this work was to compare the efficiency of two different methods for fish sampling and to assess the influence of the twilight period on the catch of gobiids along the Danube River in Serbia. The samplings were performed by electrofishing and beach seining in inshore parts of the Danube River with water depth up to 120 cm at four locations: Novi Sad, Belgrade, Tekija, and Prahovo, in October 2012 and September 2013. At each location, the samplings were performed at 17:30, 18:30, 19:30 and 20:30. Totally, 539 gobiids were caught. The highest number of specimens (218) was registered at Tekija, while lower numbers were registered at Belgrade (192) and Prahovo (117), and only 12 specimens were caught at Novi Sad. The catch of the round goby Neogobius melanostomus and monkey goby Neogobius fluviatilis was more efficient by beach seining than by electrofishing, whereas the catch of the tubenose goby Proterorhinus semilunaris was more efficient by electrofishing. Both methods had similar efficiency in catching the bighead goby Ponticola kessleri and racer goby Babka gymnotrachelus. The species diversity and number of the caught specimens were the highest at 18:30. All five species were recorded at Belgrade, Tekija and Prahovo, while only the monkey goby and racer goby were caught at Novi Sad. The methods used in this study showed good efficiency in catching gobiids, especially at dusk. Even though these methods are difficult to apply in certain habitats, they could be highly relevant in the regular monitoring of gobiids along the inshore parts of rivers

FoxP1 marks medium spiny neurons from precursors to maturity and is required for their differentiation

Identifying the steps involved in striatal development is important both for understanding the striatum in health and disease, and for generating protocols to differentiate striatal neurons for regenerative medicine. The most prominent neuronal subtype in the adult striatum is the medium spiny projection neuron (MSN), which constitutes more than 85% of all striatal neurons and classically expresses DARPP-32. Through a microarray study of genes expressed in the whole ganglionic eminence (WGE: the developing striatum) in the mouse, we identified the gene encoding the transcription factor Forkhead box protein P1 (FoxP1) as the most highly up-regulated gene, thus providing unbiased evidence for the association of FoxP1 with MSN development. We also describe the expression of FoxP1 in the human fetal brain over equivalent gestational stages. FoxP1 expression persisted through into adulthood in the mouse brain, where it co-localised with all striatal DARPP-32 positive projection neurons and a small population of DARPP-32 negative cells. There was no co-localisation of FoxP1 with any interneuron markers. FoxP1 was detectable in primary fetal striatal cells following dissection, culture, and transplantation into the adult lesioned striatum, demonstrating its utility as an MSN marker for transplantation studies. Furthermore, DARPP-32 expression was absent from FoxP1 knock-out mouse WGE differentiated in vitro, suggesting that FoxP1 is important for the development of DARPP-32-positive MSNs. In summary, we show that FoxP1 labels MSN precursors prior to the expression of DARPP-32 during normal development, and in addition suggest that FoxP1 labels a sub-population of MSNs that are not co-labelled by DARPP-32. We demonstrate the utility of FoxP1 to label MSNs in vitro and following neural transplantation, and show that FoxP1 is required for DARPP-32 positive MSN differentiation in vitro

Influence of the twilight period and different sampling methods on catch of Gobiids (Gobiidae) at four locations in the inshore parts of the Danube River

The aim of this work was to compare the efficiency of two different methods for fish sampling and to assess the influence of the twilight period on the catch of gobiids along the Danube River in Serbia. The samplings were performed by electrofishing and beach seining in inshore parts of the Danube River with water depth up to 120 cm at four locations: Novi Sad, Belgrade, Tekija, and Prahovo, in October 2012 and September 2013. At each location, the samplings were performed at 17:30, 18:30, 19:30 and 20:30. Totally, 539 gobiids were caught. The highest number of specimens (218) was registered at Tekija, while lower numbers were registered at Belgrade (192) and Prahovo (117), and only 12 specimens were caught at Novi Sad. The catch of the round goby Neogobius melanostomus and monkey goby Neogobius fluviatilis was more efficient by beach seining than by electrofishing, whereas the catch of the tubenose goby Proterorhinus semilunaris was more efficient by electrofishing. Both methods had similar efficiency in catching the bighead goby Ponticola kessleri and racer goby Babka gymnotrachelus. The species diversity and number of the caught specimens were the highest at 18:30. All five species were recorded at Belgrade, Tekija and Prahovo, while only the monkey goby and racer goby were caught at Novi Sad. The methods used in this study showed good efficiency in catching gobiids, especially at dusk. Even though these methods are difficult to apply in certain habitats, they could be highly relevant in the regular monitoring of gobiids along the inshore parts of rivers

Methods for specifying the target difference in a randomised controlled trial : the Difference ELicitation in TriAls (DELTA) systematic review

Peer reviewedPublisher PD

The Eye of the Beholder: Youths and Parents Differ on What Matters in Mental Health Services

The goal of this study was to examine the degree to which youths and caregivers attend to different factors in evaluating their experiences with mental health programs. Youth (n = 251) receiving mental health services at community agencies and their caregivers (n = 275) were asked open-ended questions regarding the positive and negative aspects of the services. Qualitative analyses revealed some agreement but also divergence between youth and caregivers regarding the criteria by which services were evaluated and aspects of services that were valued most highly. Youths’ positive comments primarily focused on treatment outcomes while caregivers focused more on characteristics of the program and provider. Youths’ negative comments reflected dissatisfaction with the program, provider, and types of services offered while caregivers expressed dissatisfaction mainly with program characteristics. Results support the importance of assessing both youth and caregivers in attempts to understand the factors used by consumers to evaluate youth mental health services

A Randomized Controlled Study of Parent-assisted Children’s Friendship Training with Children having Autism Spectrum Disorders

This study evaluated Children’s Friendship Training (CFT), a manualized parent-assisted intervention to improve social skills among second to fifth grade children with autism spectrum disorders. Comparison was made with a delayed treatment control group (DTC). Targeted skills included conversational skills, peer entry skills, developing friendship networks, good sportsmanship, good host behavior during play dates, and handling teasing. At post-testing, the CFT group was superior to the DTC group on parent measures of social skill and play date behavior, and child measures of popularity and loneliness, At 3-month follow-up, parent measures showed significant improvement from baseline. Post-hoc analysis indicated more than 87% of children receiving CFT showed reliable change on at least one measure at post-test and 66.7% after 3 months follow-up

The survival of neural precursor cell grafts is influenced by in vitro expansion

Embryonic neural precursor cells (ENPs) provide a potential alternative for transplantation in neurodegenerative diseases, as they can be expanded in culture, avoiding many of the practical obstacles that limit the application of transplanting primary neurones. However, grafts of ENPs into animal models show variable survival and limited differentiation into neurones. The effect of expansion time on their ability to survive and differentiate may be an important factor in this and has not been examined directly. In these experiments, murine and human ENPs were expanded for short (4 weeks) and long (20 weeks) periods before transplantation into the adult rat striatum. Whereas grafts of both short- and long-term expanded human ENPs survived for 4 weeks following transplantation, by 20 weeks all long-term expanded grafts had disappeared. Murine ENPs behaved similarly: only grafts of short-term expanded ENPs survived at 12 weeks following transplantation. RT-PCR analysis of ENP cultures after 4 and 20 weeks of expansion demonstrated changes in expression of a number of different groups of genes. We conclude that long-term expansion of ENPs profoundly impairs their ability to survive long-term after transplantation into the adult brain. This has implications for the potential use of these cells for neural transplantation strategies