GROWTH RESPONSE OF MAIZE (ZEA MAYS L.) TO METAL TOXICITY

This study evaluated the phytotoxic effects of lead (Pb) and zinc (Zn) as Pb(NO3)2  and Zn(NO3)2 on maize  (Zea mays L) considering the  plants’ growth indices, biomass as well as soil parameters and soil microorganisms after treatment. The results showed that plant growth significantly reduced with increasing concentrations (100,150 and 200 mgkg-1) of Pb and Zn contamination. The results indicated significant inhibitory effect on the growth parameters (stem height, root length, leaf area, fresh and dry weight) measured, compared to control experiment. This study therefore revealed that the heavy metals have harmful effects on maize’s growth.  Consequently, the consumption of such contaminated cereal by man and animals can lead to their death after the metals have biomagnified in their systems. Lead and Zn application in general, affected the vegetative growth; both fresh and dry weights were reduced with increasing concentrations of the metals. These results show that these metals are toxic to human and animal health. As a result, farmers should be encouraged to use water from good source for irrigation and also cultivate on metal-free soils if possible to save lives.     &nbsp

In-silico identification of differentially expressed genes in Type 1 diabetes mellitus

The incidence of Type 1 Diabetes Mellitus T1DM varies markedly in different geographical populations but seems to be increasing globally. The focus of this research is to screen for T1D-associated differentially expressed genes (DEGs). A meta-analysis was conducted using the Gene Expression Omnibus (GEO) datasets. The datasets included samples from T1DM and normal patients. The Robust Multichip Averaging (RMA) procedure was used for background correction, normalization and summarization to obtain expression level data and to discover differentially expressed genes. Box plots, Density plots, RNA degradation plots and recommended procedures from Affymetrix for quality control were implemented. The DEGs were screened and the exclusively expressed genes were uncovered through the Venn diagrams and heat maps functions in R language. 3,824 genes were classified, as DEGs of which 2,030 were upregulated and 1,794 were downregulated. Seven key genes (TLN1, ANPEP, F13A1, SPARC, SPTBN1, IGHA2 and IGHA1) were exclusively expressed in the whole progression. 58 DEGs were revealed through the Venn diagrams while the Heatmaps showed the differential expression data for 35 genes. IGHA1, IGHA2, IGKV4-1 were significantly expressed and upregulated. Although some of these genes have been previously associated with T1D, many other genes were identified for further studies

Surface Mixed Layer Profile of Physical and Biogeochemical Variables in the Subpolar North-West and -East Atlantic Ocean: A Data-Model Comparison Study

This paper presents a study of physical and biogeochemical variables using numerical model and mixed layer oceanographic data from a 2 - 3 year in situ measurements in the Northwestern and Northeastern sites of the Atlantic Ocean. Model outputs are presented and indicated that very good estimates may be obtained. The outputs showed considerable agreement in reproducing seasonal distributions of pCO2, pCO2-T, pCO2-nonT, mixed layer temperature, and chlorophyll-a in both winter and summer, and therefore provide useful physical and theoretical understanding of their biogeochemistry. The model pCO2 indicated a distinct temporal variability with seasonal changes coinciding with the change in sea surface temperature. It also provides an agreement that there is a strong seasonal cycle of mixed layer parameters filliped by nonthermal and physical factors. As an outgrowth of this work, the pCO2 model outputs affirm the North Atlantic Ocean capacity as an important oceanographic sink for anthropogenic carbon dioxid

Rapid diagnostic test value and implementation in antimicrobial stewardship across low-to-middle and high-income countries: a mixed-methods review

Despite technological advancements in infectious disease rapid diagnostic tests (RDTs) and use to direct therapy at the per-patient level, RDT utilisation in antimicrobial stewardship programmes (ASPs) is variable across low-to-middle income and high-income countries. Key insights from a panel of seven infectious disease experts from Colombia, Japan, Nigeria, Thailand, the UK, and the USA, combined with evidence from a literature review, were used to assess the value of RDTs in ASPs. From this, a value framework is proposed which aims to define the benefits of RDT use in ASPs, separate from per-patient benefits. Expert insights highlight that, to realise the value of RDTs within ASPs, effective implementation is key; actionable advice for choosing an RDT is proposed. Experts advocate the inclusion of RDTs in the World Health Organization Model List of essential in vitro diagnostics and in iterative development of national action plans

Farmers’ perception and Knowledge need for adoption of new cultivars of cassava in Igabi Local Government Area (LGA), Kaduna State.

The study examined farmers’ perception and knowledge need for adoption new varieties of cassava in Igabi LGA, Kaduna State. The purpose of the research was to;( 1) ascertain how cassava farmers in the area perceive the new varieties of cassava vis-a-vis the technologies required for its propagation, and (2) the relationship between the existing and recommended knowledge of cassava cultivation in the area. Seventy six respondents used for the study were selected by random sampling while focus group discussion and interview schedule were employed to gather data. Data were analyzed by mean score analysis. The result of the analysis shows that majority (36.8%) were between 30- 39 years, 89% married and 34.2% had secondary education while 50% had regular extension contact. However, out of five (5) main recommended production technologies, only two- planting methods (51.3%) and weeding interval (56.6%) were in line with farmers’ previous experience or knowledge. Farmers perceived the new cassava cultivars as beneficial through its high yielding capacity (3.3*), fast maturity (3.1) and disease resistance (2.5) among others, while capital and marketing (3.2 apiece), farm size (3.0) were among the perceived constraints to adoption of the new cultivars. The study recommended among others formation of cooperative society to aid farming activities in the area. Keywords: awareness, benefit, constraints, knowledge of planting cassava, perception

Prevalence of malaria infection among patients attending Murtala Muhammed specialist hospital Kano, Nigeria

Malaria is one of the most common diseased conditions in Nigeria and  across most developing countries triggered by one of four species of Plasmodium. The objective of this study was to screen and detect for the presence of Plasmodium species via microscopic analysis on Malaria patients attending a healthcare facility Northern Nigeria and present the epidemiological data of malaria.Finger prick blood samples, Thick and Thin Giemsa-stained blood smears, were collected from 350 malaria-suspected individuals representing all age groups. The Giemsa-stained blood smears were examined microscopically. Demographic information on rural and urban dwellings, use of  insecticides and mosquito nets were collected using structured questionnaires. Malaria cases were detected in in 227 (64.9%) of the participants with a higher infection rate amongst the males (147) than the females (80). The  predominant specie found was Plasmodium  falciparum. All age groups in this study were vulnerable in the order of 61-above>31-40>51-60>41-50>1-10>21-30> 11-20 years of age.A large number of participants dwelling in urban area (219) were tested positive for malaria in contrast to eight(8) from the rural area. Not using insecticides and mosquito treated nets were significantly associated with the prevalence of malaria as 59.4% of participants who were tested positive for  Plasmodium falciparum infection utilized insecticides, while 66.9% of those who did not were also tested positive. Individuals residing close to gutters and bushes were the most susceptible (85.6%) to Plasmodium infection.Keywords: Prevalence, Malaria, Plasmodium falciparum, Kano

Gross architecture of ovarian specimens from a southwest Nigerian tertiary healthcare facility

Background: The aim of our study is to describe the gross morphology of ovarian specimens and to see if some entities can be distinguished based on their gross morphology. Methods: We did a cross-sectional study of all ovarian specimen received by the department of morbid anatomy and forensic medicine of Obafemi Awolowo university teaching hospitals complex (OAUTHC), Ile-Ife, Nigeria from January 1, 2018, to December 31, 2022 (Five-year period). OAUTHC is situated in the southwestern part of Nigeria. Results: We examined 225 right ovarian specimens. Non- neoplastic lesions were the most common lesions of the right ovary accounting for 52 cases followed by malignant tumour accounting for 25 cases. We examined 233 specimens of the left ovary of which 133 were normal. Eighteen (72%) of malignant tumors of the left ovary had cystic cut surfaces. The spectrum of lesions of both ovaries has different average widest diameters with significant overlap in their distribution. Metastatic carcinoma is seen to involve both ovaries and found to mainly have a solid cut surface. We found that the average widest diameter of a normal left ovary is 3.6 cm while that of a normal right ovary is 4.9 cm. Conclusions: The ovarian lesions have different widest diameters and are usually larger than that of a normal ovary. Benign ovarian tumours are generally larger than their malignant counterparts but cannot be distinguished based on size alone. Metastatic carcinoma involved both ovaries and had a predominantly solid-cut surface. The normal left ovaries were smaller than the normal right ovaries

Interprofessional spiritual care education in pediatric hematology-oncology: A pilot study.

Background Evidence and clinical guidelines call care team members to address the spiritual well-being of pediatric patients, especially adolescents and young adults (AYA), with cancer and blood disorders. However, the lack of relevant training in generalist spiritual care has been a key barrier. Therefore, we aimed to improve clinicians’ capabilities by utilizing the Interprofessional Spiritual Care Education Curriculum (ISPEC) to close this gap in pediatric hematology-oncology. A model of interprofessional spiritual care entails that all team members attend to patients’ spirituality by employing generalist spiritual care skills and collaborating with spiritual care specialists such as chaplains. Methods Interdisciplinary team members providing care for AYA with cancer and blood disorders were recruited to participate in interprofessional spiritual care education. Our intervention combined an evidence-based online curriculum and in-person discussion groups. Pretest-posttest study examined changes in participants’ skills and practices to identify, address, and discuss spiritual concerns. Surveys were conducted at baseline and at 1, 3, and 6 months after the intervention. Results Participants (n = 21) included physicians, advanced practice providers, nurse coordinators, and psychosocial team members. We observed positive changes in participants’ ability (36%, P < 0.01), frequency (56%, P = 0.01), confidence (32%, P < 0.01), and comfort (31%, P = 0.02) providing generalist spiritual care baseline versus one month, with significant gains maintained through six months (Omnibus P < 0.05). Conclusions Utilizing ISPEC, interprofessional spiritual care education has a strong potential to develop pediatric hematology-oncology team members’ capabilities to attend to the spiritual aspect of whole-person care and thus contribute to the well-being of AYA with cancer and blood disorders