Расстройства сексуального здоровья при воспалительных заболеваниях внутренних гениталий у женщин

При обследовании гинекологических больных выявлены развивающиеся у них разные варианты и формы сексуальной дезадаптации супружеской пары. Показаны их причины, механизмы формирования и клинические проявления.The investigation of gynecological patients revealed development of different variants and forms of sexual dysadaptation of the married couple. Their causes, formation mechanisms and clinical manifestations are shown

Geopolymerization mechanism of binder-free mine tailings by sodium silicate

The geopolymerization of solid substances rich in calcium and aluminum including mine tailings by sodium silicate is mainly believed to be under the influence of alkali activation. However, mine tailings’ relative neutral condition can decrease sodium silicate's overall alkalinity to an unfavorable condition for alkaline reactivity. The decreased sodium silicate's alkalinity initiates the silica gel's precipitation leading to the overall mine tailings’ geopolymerization. This makes sodium silicate as an alternative binder similar to Portland cement in cemented paste backfill. Any changes in mine tailings’ chemical state can accelerate or decelerate geopolymerization bringing inadequate knowledge about strength development. Thus, mine tailings’ geopolymerization by sodium silicate was investigated at different pH values and compared with cementation by Portland cement. It was found that the elevation in sodium silicate content decreases the acidification influence of mine tailings and postpones the strength development. The setting time of geopolymerised and cemented mine tailings were comparable, but pre-acidification of sodium silicate considerably accelerated the geopolymerization process. This made sodium silicate more advantageous over Portland cement because of manageable curing behavior and strength development. The obtained experimental results were discussed through several mechanical, rheological, microstructural, mineralogical, and chemical tests and analyses. © 2020 Elsevier LtdIran National Science Foundation Tarbiat Modares UniversityThis study was accomplished through a collaboration between Tarbiat Modares, Sabanci, and Recep Tayyip Erdogan universities funded through a research grant (NO:96016805, 2018) awarded by Iran National Science Foundation (INSF)

Effects of cognitive load on inter-phase transfer of bimanual coordination patterns in elderly

Bimanual coordination is a significant ability that is used in many daily tasks including bottoming cloth, driving, picking, throwing, catching and many sport skills. Performing these activities depends on many factors such as age, gender, and attention limitations. The purpose of this research was to compare the inter-phase transfer of bimanual coordination patterns in aged men and women with/or without cognitive load when hand movements speed were gradually increased. In this experimental research, one group of healthy volunteer men (n=10) and women (n=10) aged between 60 to 74 years participated in a bimanual coordination task. The instrument used to examine the transfer phase was a set made by Pedidar-Omid Farda Co. Analysis of variance was employed to analyze the data. The result indicated that cognitive load had a significant effect on transfer phase of bimanual coordination patterns (P=0.001). However, there was no significant difference between the gender nor there was any interaction effect (P>0.05). The results of present research indicated that cognitive load had an impairing effect on bimanual coordination and causes faster speed of transfer between the patterns regardless of gender factor

Impact of exosome-loaded chitosan hydrogel in wound repair and layered dermal reconstitution in mice animal model

Combat or burn injuries are associated with a series of risks, such as microbial infection, an elevated level of inflammatory response, and pathologic scar tissue formation, which significantly postpone wound healing and also lead to impaired repair. Skin engineering for wound healing requires a biomimetic dressing substrate with ideal hydrophilicity, holding antioxidant and antimicrobial properties. In addition, available bioactive specification is required to reduce scar formation, stimulate angiogenesis, and improve wound repair. In this study, we successfully fabricated chitosan (Ch)–based hydrogel enriched with isolated exosome (EXO) from easy-accessible stem cells, which could promote fibroblast cell migration and proliferation in vitro. Full-thickness excisional wound model was used to investigate the in vivo dermal substitution ability of the fabricated hydrogel composed Ch and EXO substrates. Our finding confirmed that the wounds covered with Ch scaffold containing isolated EXO have nearly 83.6 wound closure ability with a high degree of re-epithelialization, whereas sterile gauze showed 51.5 of reduction in wound size. In summary, obtained results imply that Ch-glycerol-EXO hydrogel construct can be utilized at the full-thickness skin wound substitution and skin tissue engineering. © 2020 Wiley Periodicals LLC

TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes

Transforming acidic coiled-coil (TACC) proteins are key players during mitosis via stabilization of the spindle. The roles of TACCs during meiosis are however less clear. We used bovine oocytes to study the expression and function of TACC3 during meiosis. TACC3 mRNA was detected in bovine oocytes during meiosis using qRT-PCR, and while it was also expressed in cleavage stage embryos, its expression was down-regulated at the morula and blastocyst stages. Immunofluorescence was used to demonstrate that TACC3 co-localized with tubulin in the metaphase I and II spindles. However, TACC3 was not detected at anaphase or telophase of the first meiotic division. Aurora A, which is known to phosphorylate and activate TACC3 in mitotic cells, showed a similar pattern of gene expression to that of TACC3 in meiotic oocytes and preimplantation embryos. Aurora A protein was however only very transiently associated to the meiotic spindle. Pharmaceutical inhibition of Aurora A activity inhibited TACC3 phosphorylation but did not prevent TACC3 appearance in the spindle. Inhibiting Aurora A activity did however lead to abnormal meiotic spindle formation and impaired maturation of bovine oocytes. Similar results were obtained by knock-down of TACC3 expression using siRNA injection. These results suggest that TACC3 is important for stabilizing the meiotic spindle, but phosphorylation of TACC3 by Aurora A is not required for its recruitment to the meiotic spindle although phosphorylation of TACC3 by other kinases cannot be excluded

TACC3 co-localization with tubulin.

<p>TACC3 co-localizes with tubulin in the spindle at metaphase I, is not expressed at anaphase and reappearance at telophase. TACC3 is not present in the polar body. (A-C) metaphase I stage, (D) anaphase stage, (E) telophase stage and (F) metaphase II stage; blue = DAPI/DNA. Red = TACC3 and green = α-tubulin; Scale bar = 10 μM.</p

TACC3 localization in meiotic oocytes as detected by using immunofluorescence.

<p>Each row of three images are in the order; DAPI staining, TACC3 staining and overlay, and correspond to the same developmental time point. (A) 0 h, germinal vesicle stage; (B) 6 h of maturation; (C) 9 h; (D) 12 h, metaphase I stage; (E) 21 h, metaphase II stage. Arrow = first polar body. Scale bar = 20 μm.</p

TACC3 expression after exposure of bovine oocytes to MLN8054 during maturation <i>in vitro</i>.

<p>Shown are cells at metaphase I (upper row) and metaphase II (bottom row) cultured at the indicated concentrations of MLN8053. TACC3 expression in red is detected with immunostaining; blue represents DNA (DAPI). Scale bar = 10μM.</p