Multiplex PCR Method of Detecting Pork to Guarantee Halal Status in Meat Processed Products

One of the halal parameters of food is must be free from pork among from the basic ingredients, additives and the manufacturing process. The aim of this study was ensure the halal status in the form of presence or absence of pork in processed meat product (meatballs and sausages) in traditional markets in Pandeglang Regency. PCR multiplex was a PCR technique that used several primers together in one reaction to amplify several target sequence. The genes often used as markers of animal or meat types include cytochrome b (cyt b), the existence of sequence variations in cyt b causes these genes are widely used as markers to distinguish material from different types of animals. The results showed that the cyt b gene proved successful in amplified DNA from beef and pork with different fragment lengths in the DNA mix of the 2 types of livestock in one reaction so that 2 DNA bands formed with different lengths according to the length of the fragment from each animal. From the results of research showed beef and pork control there was two fragments such as 274 bp for beef and 389 bp for pork. Multiplex PCR testing on meatball samples showed that the meatballs were tested 100% positive containing beef and 0% containing pork DNA. While testing on sausage products, there were one sausage brand that showed the results of DNA amplification of 398pb, which means the product was positive containing pork

Perbandingan Tumbuh Kembang Anak Toddler yang Diasuh Orang Tua dengan Diasuh Selain Orang Tua

Growth and development of child will be different between parent's care and other's care. The aim of this research was to determined the differences of growth and development of toddler who cared by parents and cared by others. Design of this research was analytic comparative. This research had been done in Sidomulyo Barat, Tampan district with 84 respondents, using cross sectional approach. The samples were taken by cluster sampling technique. The instruments are scales and gauges to the measure of weight and height, whereas to development of child used Denver Development Screening Test (DDST). Analysis data used univariate and bivariate analysis. Characteristic of respondents was analyzed by univariate and correlation between variables was analyzed by chi-square test. The results showed that there was differences between parent's care and other's care. Toddlers who cared by parent have better growth and development than care by others (p value < α). This research recommends to parents or child care provider to provide a good care, so the children will having growth and development optimally

Efektivitas Air Rebusan Daun Sirih Merah terhadap Penurunan Kadar Gula Darah pada Pasien Diabetes Melitus di Wilayah Kerja Puskesmas Saling 2018

Diabetes Melitus merupakan penyakit kronik yang terjadi akibat pankreas tidak mampu menghasilkan insulin yang cukup atau ketika tubuh tidak dapat secara efektif menggunakan insulin tersebut. Tujuan penelitian ini adalah untuk mempelajari efektivitas pemberian air rebusan daun sirih merah terhadap penurunan kadar gula darah pasien Diabetes Melitus di Wilayah Kerja Puskesmas Saling Kabupaten Empat Lawang Tahun 2018. Desain penelitian ini adalah Quasy eksperimental dengan menggunakan rancangan The One Group Pretest – Postest Design, teknik pengambilan data menggunakan data primer dan sekunder. Populasi penelitian ini adalah seluruh pasien Diabetes Melitus di Puskesmas Saling pada bulan Juli-Agustus tahun 2018 berjumlah 16 orang, pengambilan sampel dilakukan dengan Accidental sampling. Hasil penelitian, dari 16 penderita Diabetes Melitus terdapat 9 orang (56.2%) dengan kadar gula darah kurang dari 200 mg/dl. Hasil uji Wilcoxon Sign Rank didapat nilai Z=-3,517 dengan p=0,000<0,05 berarti signifikan. Jadi kedua variabel memiliki median yang berbeda, ada perbedaan Kadar GDS pasien Diabetes Melitus sebelum dan setelah pemberian air rebusan daun sirih merah. Kesimpulan, Air rebusan daun sirih merah efektif secara signifikan terhadap penurunan kadar gula darah pada pasien diabetes melitus. Diharapkan bagi petugas Puskesmas agar kiranya dapat meningkatkan pengetahuan masyarakat dengan cara penyuluhan tentang daun sirih merah sebagai obat Non Farmakologi bagi penderita Diabetes Melitus yang tidak mengalami komplikasi

The Stock Price Prediction Formula Using the Concept of Equality in the Amount of Data Between the Average Difference of Order One and Two at Levels n and n+1

Technological developments are getting faster, as is the dissemination of existing information, especially on the capital market. In order for investors to avoid losses from the capital market, a method is needed that is able to analyze the movement of the stock price. This study focuses on the application of the Data Scales Analysis (DSA) method which uses a formula with the concept of the same amount of data between the first and second order average differences at levels n and n + 1 for predicting the stock price of issuers, in predicting stock prices in the capital market. The resulting formula is named JIC-FLY 2 which is a new formula used to predict stock prices in the capital market. The population used in this study are issuers who are members of IDX 30 from the banking sub-sector with the sample used is the issuer of BBCA (PT Bank Central Asia Tbk). The results of this study note that the DSA method with this formula is able to produce the best predictive value, namely DSA 12 with an error percentage of 0.035%

Pengaruh Penambahan Tepung Daun Kelor pada Ransum terhadap Kandungan Nutrisi dan Fisik Telur Itik

The aim of this study were to analyzed effect of adding Moringa leaf meal on feed in egg quality of duck.The livestock used were 27 ducks with 3 treatments and 3 replication and each replication there were 3ducks. P0 (feed + 0% Moringa), P1 (feed + 5% Moringa) and P2 (feed + 10% Moringa). Experimentaldesign used completely randomized design. Variables observed in this study were physical quality such asegg weight and yolk color score and chemical quality such as cholesterol and protein level. Observationwas carried out after 4 weeks of feed treatment. Significantly different result were seen in egg weightvariables (P&lt;0.05), treatment of 5% and 10% moringa leaf in feed significanty decrease weight of eggs.Adding 5% and 10% moringa leaves increased a yolk color score, respectively 7.11 + 1.05 and 7.44 +0.52 compared with control 6.55 + 3.20. Cholesterol levels decrease in 5% feed treatment 14.07 + 2.07compared to control 15.46 + 1.43. The adition of 5% and 10% moringa leaves increased protein level10.90% and 10.86% respectively, compared to control 10.74%. However, base on statistical analysis ofyolk color score, cholesterol and protein levels were not significantly different (P&gt;0.05)

PERANAN STRUKTUR PENGENDALIAN INTERN DIBIDANG PERKREDITAN DALAM MENGHINDARI KREDIT BERMASALAH PADA PT. BANK XYZ KANTOR CABANG SURABAYA

Berdasarkan hasil penelitian dan pembahasan tentang struktur pengendalian intern krOOit yang telah dilakukan pada Bank XYZ Cabang Surabaya belum cukup memadai untuk mencegah terjadinya kegagalan perkreditan. Hal ini dapat disimpulkan sebagai berikut : Posisi Per 31 Maret 1999, Kredit yang di salurkan Bank XYZ Cabang Surabaya dengan klasifikasi Perhatian Khusus 9,65%, Kurang Lancar 5,44%, Diragukan 5,34% dan Macet 6,03%, hams segera diselesaikan dengan memberikan solusi penyelamatan krOOit agar dengan berjalannya dari waktu ke waktu kredit-kredit tersebut tidak semakin memburuk dengan beralih ke kategori Macet sehingga akan mengakibatkan Kriteria Penilaian Tingkat Kesehatan Kredit Cabang menjadi Kurang Sehat

Disaster victim identification operations with fragmented burnt or commingled remains: experienced based recommendations

Human-made and natural disasters can result in severely fragmented, compromised, and commingled human remains. The related disaster victim identification (DVI) operations are invariably challenging, with the state of the remains potentially precluding some identifications. Practitioners involved in these DVI operations will routinely face logistical, practical, and ethical challenges. This review provides information and guidance derived from first-hand experiences to individuals tasked with managing DVI operations with fragmented human remains. We outline several key issues that should be addressed during disaster preparedness planning and at the outset of an operation, when incident-specific strategies are developed. Specific challenges during recovery and examination of fragmented remains are addressed, highlighting the importance of experienced specialists at the scene and in the mortuary. DNA sample selection and sampling techniques are reviewed, as well as downstream effects of commingling and contamination, which can complicate reconciliation and emphasise the need for rigorous quality control. We also touch on issues that may arise during communication with families. While recommendations are provided, they are not intended as proscriptive policy but rather as an addition to the general recommendations given in the International Criminal Police Organization (INTERPOL) DVI Guide, to inform preparative discussions between government officials, judiciary, police, and forensic specialists

Edukasi Penggunaan Masker dan CTPS dalam Pencegahan Covid-19 di Panti Asuhan RAI

Abstrak Pandemi Covid-19 telah mengubah pendekatan pengobatan ke arah pencegahan untuk mengurangi faktor risiko, penekan kasus morbiditas dan mortalitas. Vaksinasi hanya salah satu upaya untuk meningkatkan imunitas di masyarakat. Upaya pencegahan penularan sangat diperlukan untuk melindungi populasi yang rentan seperti panti asuhan. Kendala yang dihadapi mitra adalah penerapan cuci tangan dan penggunaan masker belum dilakukan secara benar, keterbatasan masker dan fasilitas hygiene perorangan. Tujuan pengabdian kepada masyarakat (PKM) yaitu memberikan edukasi penggunaan masker dan cuci tangan pakai sabun (CTPS) serta penyediaan masker dan fasilitas cuci tangan di Panti Asuhan Rumah Anak Iman (RAI) Kota Kupang. Metode yang digunakan adalah penyuluhan dan simulasi penggunaan masker dan CTPS. Peserta PKM terdiri dari 3 orang pengasuh dan 27 anak asuh yang hadir dan berpartisipasi aktif selama kegiatan. Hasil post test setelah kegiatan menunjukkan peningkatan pengetahuan dan keterampilan (praktek) sebesar 38% dari 62% hasil pre test pada awal kegiatan. Pada akhir kegiatan keseluruhan peserta sudah dapat mempraktekkan 7 langkah CTPS dengan benar dan mempunyai pemahaman yang benar tentang cara menggunakan dan menbersihkan masker kain agar tetap aman dan efektif digunakan. PKM ini sangat bermanfaat untuk mencegah penularan Covid-19 pada kelompok risiko serta menggerakan partisipasi masyarakat dalam upaya promosi dan preventif Covid-19. &nbsp; &nbsp;Abstract&nbsp; &nbsp;&nbsp;&nbsp; Nowdays, health service of Pandemi Covid 19 has put more attention to reducing of risk factors causing increasing of morbidity and mortality. Vaccination is only one of the efforts to increase immunity in the community. Efforts to prevent transmission are needed to protect vulnerable populations such as orphanages. Constraints faced by partners are the application of hand washing and the use of masks that have not been carried out properly, the limitations of masks and personal hygiene facilities. The purpose of community service were activities to provide education on the use of masks and hand washing with soap as well as the provision of masks and hand washing facilities at the Rumah Anak Iman (RAI) Orphanageo Kupang City. The method use was counseling and simulation of the use of masks and hand washing with soap. The activity participants consisted of 3 caregivers and 27 foster children who were present and actively participated during the activity. The post test results after the activity showed an increase in knowledge and skills (practice) by 38% from 62% of the pretest results at the beginning of the activity. At the end of the activity, all participants were able to practice the seven steps of hand washing with soap correctly and have the correct understanding of how to use and clean cloth mask to make it reusable. This activity is very useful to prevent the transmission of Covid-19 in risk groups and to mobilize community participation in the promotion and prevention of Covid-19. &nbsp

PHENOMENOLOGICAL STUDY: QUALITY OF LIFE OF ADOLESCENT WITH CANCER

Introduction. Adolescent is a period growth and development between children and adult. Cancer diagnosed at adolescent age can made them under pressure and full of stress. The aim of the study was to explore a deeper description about quality of life of adolescent with cancer. Method. The study used phenomenology descriptive design with purposive sampling. In-depth interview was held on six adolescent with cancer. Transcript verbatim was analyzed using Colaizzi. Result. The themes were dimension of physical, cognitive, psychology, social, adaptive coping mechanism, and hope. The study concluded that adolescent with cancer dealing with many changing on quality of life but some can build adaptive coping mechanism with their condition and increased their quality of life. Discussion. Child nurse have a big role to increase quality of life of adolescent with cancer by giving a holistic and comprehensive nursing care.Keyword: adolescent, cancer, quality of life, phenomenolog