Relative proximity of chromosome territories influences chromosome exchange partners in radiation-induced chromosome rearrangements in primary human bronchial epithelial cells

Copyright © 2013 The Authors. This article is made available through the Brunel Open Access Publishing Fund. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.Copyright © 2013 The Authors. It is well established that chromosomes exist in discrete territories (CTs) in interphase and are positioned in a cell-type specific probabilistic manner. The relative localisation of individual CTs within cell nuclei remains poorly understood, yet many cancers are associated with specific chromosome rearrangements and there is good evidence that relative territorial position influences their frequency of exchange. To examine this further, we characterised the complexity of radiation-induced chromosome exchanges in normal human bronchial epithelial (NHBE) cells by M-FISH analysis of PCC spreads and correlated the exchanges induced with their preferred interphase position, as determined by 1/2-colour 2D-FISH analysis, at the time of irradiation. We found that the frequency and complexity of aberrations induced were reduced in ellipsoid NHBE cells in comparison to previous observations in spherical cells, consistent with aberration complexity being dependent upon the number and proximity of damaged CTs, i.e. lesion proximity. To ask if particular chromosome neighbourhoods could be identified we analysed all radiation-induced pair-wise exchanges using SCHIP (statistics for chromosome interphase positioning) and found that exchanges between chromosomes (1;13), (9;17), (9;18), (12;18) and (16;21) all occurred more often than expected assuming randomness. All of these pairs were also found to be either sharing similar preferred positions in interphase and/or sharing neighbouring territory boundaries. We also analysed a human small cell lung cancer cell line, DMS53, by M-FISH observing the genome to be highly rearranged, yet possessing rearrangements also involving chromosomes (1;13) and (9;17). Our findings show evidence for the occurrence of non-random exchanges that may reflect the territorial organisation of chromosomes in interphase at time of damage and highlight the importance of cellular geometry for the induction of aberrations of varying complexity after exposure to both low and high-LET radiation.Department of Healt

Bioorthogonal Double-Fluorogenic Siliconrhodamine Probes for Intracellular Superresolution Microscopy

A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of genetically manipulated intracellular proteins and subsequent imaging using super-resolution microscopy

Icy thermometers : quantifying the impact of volcanic heat on glacier elevation

ACKNOWLEDGEMENTS This project was supported by the NERC Global Partnerships Seedcorn grant NE/W003724/1 and the Leverhulme Trust Research Project RPG-2019-093. We thank Kevin A. Reath for support in analysing volcanic thermal anomalies. A.G.P. and M.E.P. were partly supported by the NASA Science Mission Directorate Earth Surface and Interior grant 80NSSC21K0842. J.J. was supported by the NERC Quadrat Doctoral Training Partnership. We express our gratitude to John Smellie and an anonymous reviewer for fruitful feedback which greatly improved the manuscript.Peer reviewedPostprin

Electrification of Sub-Saharan Africa through PV/hybrid mini-grids: Reducing the gap between current business models and on-site experience

The absence of publicly available up-to-date costs breakdown data on photovoltaic (PV)/hybrid mini-grids in Sub-Saharan Africa (SSA) is a barrier that needs to be resolved in order to overcome challenges in rural electrification planning, regulation, life-cycle operation, financing, and funding. The primary aim of this research is to provide better understanding of the cost structures of PV/hybrid mini-grid projects in Sub-Saharan Africa. The review on existing literature reveals significant lack of transparency and inconsistencies in PV/hybrid mini-grid costs. This paper aims to support the fact that there still remains a strong need to reduce the gap between current business model concepts and successfully implemented scale-up electrification models. Based on the experience of PV/hybrid mini-grids projects implemented in various rural communities of SSA, we propose a multi-dimensional cost analysis with a standardised break-down of the real costs of installed projects. Subsequently, we assess the main social and environmental implications and we identify barriers that appear to hinder successful PV mini-grid planning and subsequent implementation in SSA. Africa has the unique opportunity to utilize renewable energy as a primary energy source. Indeed, the continent has the potential to bring electricity especially to its rural population by means of PV/hybrid mini-grids. However, the capability of public and private sector investors to preevaluate projects is limited by the lack of locally available information on PV/hybrid mini-grid costs or the reliability of data (when available). Multi-dimensional cost analysis of social and environmental impacts from this study highlight that PV/hybrid mini-grids offer a unique opportunity to create a standardised framework for quantifying costs of PV/hybrid mini-grids in SSA, that can support decision-making processes for designing viable business models. Findings show that there is a strong need to minimise the data quality gap between current business model and that of successfully implemented PV/hybrid mini-grids electrification projects. This gap could be mitigated through studying the issues that influence mini-grid costs (both hardware and software). In addition to understanding other factors that can influence project costs such as the market maturity and remoteness of the site, organisation capability, development approach, and level of community involvement. Regarding policy considerations, stronger political will coupled with proactive rural electrification strategies and targeted renewable energy regulatory framework would be essential in order to establish viable dynamic domestic market for off grid renewables. In the presented benchmarking analysis, the experiences of public and private development organisations are synchronized to contribute to the furthest extent possible to facilitate the assessment. Those include the disaggregation of component costs according to their unit in order to make comparison more accurate and include site-specific parameters in the discussion of costs.JRC.C.2-Energy Efficiency and Renewable

Light-triggered carotenogenesis in myxococcus xanthus: New paradigms in photosensory signaling, transduction and gene regulation

22 pags., 6 figs. -- This article belongs to the Special Issue Myxobacteria: Physiology and RegulationMyxobacteria are Gram-negative δ-proteobacteria found predominantly in terrestrial habitats and often brightly colored due to the biosynthesis of carotenoids. Carotenoids are lipophilic isoprenoid pigments that protect cells from damage and death by quenching highly reactive and toxic oxidative species, like singlet oxygen, generated upon growth under light. The model myxobacterium Myxococcus xanthus turns from yellow in the dark to red upon exposure to light because of the photoinduction of carotenoid biosynthesis. How light is sensed and transduced to bring about regulated carotenogenesis in order to combat photooxidative stress has been extensively investigated in M. xanthus using genetic, biochemical and high-resolution structural methods. These studies have unearthed new paradigms in bacterial light sensing, signal transduction and gene regulation, and have led to the discovery of prototypical members of widely distributed protein families with novel functions. Major advances have been made over the last decade in elucidating the molecular mechanisms underlying the light-dependent signaling and regulation of the transcriptional response leading to carotenogenesis in M. xanthus. This review aims to provide an up-to-date overview of these findings and their significance.This research was funded by grants PGC2018-094635-B-C21 (to M.E.-A.) and PGC2018- 094635-B-C22 (to S.P) from the Agencia Estatal de Investigación (AEI)-Spain and European Regional Development Fund (FEDER), and by grant 20992/PI/18 (to M.E.-A.) from Fundación Séneca (Murcia)- Spain. The Ministerio de Educación y Cultura-Spain funded Ph.D. fellowships to A.J.M.-G, E.P.-M. and E.B.-M., and AEI-Spain funded that to R.P.-C

Intermittency of near-bottom turbulence in tidal flow on a shallow shelf

The higher-order structure functions of vertical velocity fluctuations (transverse structure functions (TSF)) were employed to study the characteristics of turbulence intermittency in a reversing tidal flow on a 19 m deep shallow shelf of the East China Sea. Measurements from a downward-looking, bottom-mounted Acoustic Doppler Velocimeter, positioned 0.45 m above the seafloor, which spanned two semidiurnal tidal cycles, were analyzed. A classical lognormal single-parameter (mu) model for intermittency and the universal multifractal approach (specifically, the two-parameter (C-1 and alpha) log-Levy model) were employed to analyze the TSF exponent xi(q) in tidally driven turbulent boundary layer and to estimate mu, C-1, and alpha. During the energetic flooding tidal phases, the parameters of intermittency models approached the mean values of (mu) over tilde approximate to 0.24, (C) over tilde (1) approximate to 0.15, and (alpha) over tilde approximate to 1.5, which are accepted as the universal values for fully developed turbulence at high Reynolds numbers. With the decrease of advection velocity, mu and C-1 increased up to mu approximate to 0.5-0.6 and C-1 approximate to 0.25-0.35, but a decreased to about 1.4. The results explain the reported disparities between the smaller "universal" values of intermittency parameters mu and C-1 (mostly measured in laboratory and atmospheric high Reynolds number flows) and those (mu = 0.4-0.5) reported for oceanic stratified turbulence in the pycnocline, which is associated with relatively low local Reynolds numbers R-lambda w. The scaling exponents xi(2) of the second-order TSF, relative to the third-order structure function, was also found to be a decreasing function of R-lambda w, approaching the classical value of 2/3 only at very high R-lambda w. A larger departure from the universal turbulent regime at lower Reynolds numbers could be attributed to the higher anisotropy and associated intermittency of underdeveloped turbulence.U.S. Office of Naval Research [N00014-05-1-0245]; Spanish Ministry of Education and Science [FIS2008-03608]; Major State Program of China for Basic Research [2006CB400602]; Catalan Institute for Water Research (ICRA