Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site.

Chromosome segregation begins when the cysteine protease, separase, cleaves the Scc1 subunit of cohesin at the metaphase-to-anaphase transition. Separase is inhibited prior to metaphase by the tightly bound securin protein, which contains a pseudosubstrate motif that blocks the separase active site. To investigate separase substrate specificity and regulation, here we develop a system for producing recombinant, securin-free human separase. Using this enzyme, we identify an LPE motif on the Scc1 substrate that is distinct from the cleavage site and is required for rapid and specific substrate cleavage. Securin also contains a conserved LPE motif, and we provide evidence that this sequence blocks separase engagement of the Scc1 LPE motif. Our results suggest that rapid cohesin cleavage by separase requires a substrate docking interaction outside the active site. This interaction is blocked by securin, providing a second mechanism by which securin inhibits cohesin cleavage

A spatiotemporal analysis of the relationship between near-surface air temperature and satellite land surface temperatures using 17 years of data from the ATSR series

The relationship between satellite land surface temperature (LST) and ground-based observations of 2m air temperature (T2m) is characterised in space and time using >17 years of data. The analysis uses a new monthly LST climate data record (CDR) based on the Along-Track Scanning Radiometer (ATSR) series, which has been produced within the European Space Agency GlobTemperature project (http://www.globtemperature.info/). Global LST-T2m differences are analysed with respect to location, land cover, vegetation fraction and elevation, all of which are found to be important influencing factors. LSTnight (~10 pm local solar time, clear-sky only) is found to be closely coupled with minimum T2m (Tmin, all-sky) and the two temperatures generally consistent to within ±5 °C (global median LSTnight- Tmin= 1.8 °C, interquartile range = 3.8 °C). The LSTday (~10 am local solar time, clear-sky only)-maximum T2m (Tmax, all-sky) variability is higher (global median LSTday- Tmax= -0.1°C, interquartile range = 8.1 °C) because LST is strongly influenced by insolation and surface regime. Correlations for both temperature pairs are typically >0.9 outside of the tropics. The monthly global and regional anomaly time series of LST and T2m – which are completely independent data sets - compare remarkably well. The correlation between the data sets is 0.9 for the globe with 90% of the CDR anomalies falling within the T2m 95% confidence limits. The results presented in this study present a justification for increasing use of satellite LST data in climate and weather science, both as an independent variable, and to augment T2m data acquired at meteorological stations

Cloud clearing techniques over land for land surface temperature retrieval from the Advanced Along Track Scanning Radiometer

We present five new cloud detection algorithms over land based on dynamic threshold or Bayesian techniques, applicable to the Advanced Along Track Scanning Radiometer (AATSR) instrument and compare these with the standard threshold based SADIST cloud detection scheme. We use a manually classified dataset as a reference to assess algorithm performance and quantify the impact of each cloud detection scheme on land surface temperature (LST) retrieval. The use of probabilistic Bayesian cloud detection methods improves algorithm true skill scores by 8-9 % over SADIST (maximum score of 77.93 % compared to 69.27 %). We present an assessment of the impact of imperfect cloud masking, in relation to the reference cloud mask, on the retrieved AATSR LST imposing a 2 K tolerance over a 3x3 pixel domain. We find an increase of 5-7 % in the observations falling within this tolerance when using Bayesian methods (maximum of 92.02 % compared to 85.69 %). We also demonstrate that the use of dynamic thresholds in the tests employed by SADIST can significantly improve performance, applicable to cloud-test data to provided by the Sea and Land Surface Temperature Radiometer (SLSTR) due to be launched on the Sentinel 3 mission (estimated 2014)

Quantifying uncertainty in satellite-retrieved land surface temperature from cloud detection errors

Clouds remain one of the largest sources of uncertainty in remote sensing of surface temperature in the infrared, but this uncertainty has not generally been quantified. We present a new approach to do so, applied here to the Advanced Along-Track Scanning Radiometer (AATSR). We use an ensemble of cloud masks based on independent methodologies to investigate the magnitude of cloud detection uncertainties in area-average Land Surface Temperature (LST) retrieval. We find that at a grid resolution of 625 km^2 (commensurate with 0.25 degrees grid size at the tropics), cloud detection uncertainties are positively correlated with cloud-cover fraction in the cell, and are larger during the day than at night. Daytime cloud detection uncertainties range between 2.5 K for clear-sky fractions of 10-20 % and 1.03 K for clear-sky fractions of 90-100 %. Corresponding nighttime uncertainties are 1.6 K and 0.38 K respectively. Cloud detection uncertainty shows a weaker positive correlation with the number of biomes present within a grid cell, used as a measure of heterogeneity in the background against which the cloud detection must operate (eg. surface temperature, emissivity and reflectance). Uncertainty due to cloud detection errors is strongly dependent on the dominant land cover classification. We find cloud detection uncertainties of magnitude 1.95 K over permanent snow and ice, 1.2 K over open forest, 0.9-1 K over bare soils and 0.09 K over mosaic cropland, for a standardised clear-sky fraction of 74.2 %. As the uncertainties arising from cloud detection errors are of a significant magnitude for many surface types, and spatially heterogeneous where land classification varies rapidly, LST data producers are encouraged to quantify cloud-related uncertainties in gridded products

Rotary atomizer drop size distribution database

Wind tunnel measurements of drop Size distributions from Micronair A U4000 and A U5000 rotary atomizers were collected to develop a database for model use. The measurements varied tank mix, flow rate, air speed, and blade angle conditions, which were correlated by multiple regressions (average R-2 = 0.995 for A U4000 and 0.988 for AU5000). This database replaces an outdated set of rotary atomizer data measured in the 1980s by the USDA Forest Service and fills in a gap in data measured in the 1990s by the Spray Drift Task Force. Since current USDA Forest Service spray projects rely on rotary atomizers, the creation of the database (and its multiple regression interpolation) satisfies a need seen for ten years

IPC2008-64295 RECENT DEVELOPMENTS OF HIGH STRENGTH LINEPIPE STEELS ON COIL

ABSTRACT The present paper deals with the recent steel development for linepipe applications at ArcelorMittal. With the recent upgrade of the downcoiler capabilities in three European steel plants it is now possible to supply hot rolled coils in width up to 2150mm for the economical production of X70 large diameter, spiral welded transmission trunk lines with 25.4mm wall thickness. In view of the increased dimensional feasibility range a couple of new product development projects had been launched. Two of these products with very demanding application properties are already being industrialized. It concerns respectively X70 in 22mm thickness with improved low temperature BDWTT properties (85% shear area at -10°C) and X80 in 16mm, likewise with low temperature BDWTT properties and, additionally, with low Y/T ratio. The first preseries deliveries have been successfully transformed to pipes in one of our client's spiral welded pipe mill. Another product development project which is still in the conception phase concerns the development of X100 in thickness up to 12.7mm. The actual progress of the mentioned projects will be documented here. Key for the realization of such demanding products is a precise control of the final microstructure. This is in particular true for high strength steels with improved toughness. Therefore, a lot of research efforts were spent in order to understand the link between microstructures and properties. Our current understanding will be detailed in this paper

Identifying and Characterizing Impact Melt Outcrops in the Nectaris Basin

The Nectaris Basin is an 820-km diameter, multi-ring impact basin located on the near side of the Moon. Nectaris is a defining stratigraphic horizon based on relationships between ejecta units, giving its name to the Nectarian epoch of lunar history. Lunar basin chronology based on higher resolution LRO imagery and topography, while assigning some important basins like Serenitatis to pre-Nectarian time, were generally consistent with those previously derived. Based on this stratigraphy, at least 11 large basins formed in the time between Nectaris and Imbrium. The absolute age of Nectaris, therefore, is a crucial marker in the lunar time-stratigraphic sequence for understanding the impact flux on the Moon, and by extension, the entire inner solar system. For several decades, workers have attempted to constrain the age of the Nectaris basin through radiometric dating of lunar samples. However, there is little agreement on which samples in our collection represent Nectaris, if any, and what the correct radiometric age of such samples is. The importance of the age of Nectaris goes far beyond assigning a stratigraphic marker to lunar chronology. Several dynamical models use Nectaris as their pin date, so that this date becomes crucial in understanding the time-correlated effects in the rest of the solar system. The importance of the Nectaris basin age, coupled with its nearside, mid-latitude location, make remnants of the impact-melt sheet an attractive target for a future mission, either for in-situ dating or for sample return. We have started exploring this possibility. We have begun a consortium data-analysis effort bringing multiple datasets and analysis methods to bear on these putative impact-melt deposits to characterize their extent, elemental composition and mineralogy, maturity and geologic setting, and to identify potential landing sites that meet both operational safety and science requirements

‘Not a country at all’: landscape and Wuthering Heights

This article explores the issue of women’s representational genealogies through an analysis of Andrea Arnold’s 2011 Wuthering Heights. Beginning with 1970s feminist arguments for a specifically female literary tradition, it argues that running through both these early attempts to construct an alternative female literary tradition and later work in feminist philosophy, cultural geography and film history is a concern with questions of ‘alternative landscapes’: of how to represent, and how to encounter, space differently. Adopting Mary Jacobus’ notion of intertextual ‘correspondence’ between women’s texts, and taking Arnold’s film as its case study, it seeks to trace some of the intertextual movements – the reframings, deframings and spatial reorderings – that link Andrea Arnold’s film to Emily Brontë’s original novel. Focusing on two elements of her treatment of landscape – her use of ‘unframed’ landscape and her focus on visceral textural detail – it points to correspondences in other women’s writing, photography and film-making. It argues that these intensely tactile close-up sequences which puncture an apparently realist narrative constitute an insistent presence beneath, or within, the ordered framing which is our more usual mode of viewing landscape. As the novel Wuthering Heights is unmade in Arnold’s adaptation and its framings ruptured, it is through this disturbance of hierarchies of time, space and landscape that we can trace the correspondences of an alternative genealogy