Evaluation of the cytotoxicity interactions between epirubicin and daunorubicin in HeLa cell cultures

Epirubicin and Daunorubicin, antibiotics which are derivative of anthracyclines, are used on cancer chemotherapy. In this study, Epirubicin and Daunorubicin effects on cell kinetics parameters wereexamined both single and in combination on HeLa cell culture that was taken from human servical carcinoma. The experiments tested by using IC90 doses of Epirubicin and Daunorubicin (0.5 g/ml and0.1 g/ml, respectively) were applied for 24 and 48 h. Cell kinetics parameters such as growth rate (WST-1 colorimetric assay), mitotic and apoptotic index were applied to identify cytotoxicity that wasformed by drugs. In addition, DNA degradation was examined in agarose gel electrophoresis. The cytotoxic effect of drugs appeared significant decline of growth rate and mitotic index, and significantincrease of apoptotic index of HeLa cell line (

Evaluation of the cytotoxicity interactions between epirubicin and daunorubicin in HeLa cell cultures

Epirubicin and Daunorubicin, antibiotics which are derivative of anthracyclines, are used on cancer chemotherapy. In this study, Epirubicin and Daunorubicin effects on cell kinetics parameters wereexamined both single and in combination on HeLa cell culture that was taken from human servical carcinoma. The experiments tested by using IC90 doses of Epirubicin and Daunorubicin (0.5 g/ml and0.1 g/ml, respectively) were applied for 24 and 48 h. Cell kinetics parameters such as growth rate (WST-1 colorimetric assay), mitotic and apoptotic index were applied to identify cytotoxicity that wasformed by drugs. In addition, DNA degradation was examined in agarose gel electrophoresis. The cytotoxic effect of drugs appeared significant decline of growth rate and mitotic index, and significant increase of apoptotic index of HeLa cell line (

Fostering an EU strategy for security sector reform in the Mediterranean: learning from Turkish and Palestinian police reform experiences

In the absence of an overarching strategy, Turkey and the Palestinian territories depict how the EU has adopted different approaches to security sector reform (SSR) which have not facilitated the consolidation of a common EU foreign policy, though the situation might soon change given respective SSR-related documents from the Council and Commission. This report contributes to the security sector debate by stressing that in conflict and post-conflict scenarios endurable SSR requires fomenting synergies between the police and judicial sectors and the inclusion of DDR, in tandem with the institutional implementation of transparent, accountable and democratic oversight mechanisms. There is an adamant need for constructive consistency when applying this central facet of EU foreign policy in the Mediterranean basin and beyond. SSR is an emerging phenomenon in conflict, post-conflict and development scenarios that has acquired a prominent role within the policy agendas of key international actors. As a prelude to the two case studies, and in order to better understand the EU’s end goal, a brief analysis of the two pivotal European SSR documents is provided with particular emphasis on their contribution to develop a more coherent and effective EU presence in this field. More specific consideration is then given to the role played by SSR-related matters within the framework of two EU foreign policy mechanisms towards the Mediterranean: the Euro-Mediterranean Partnership (EMP), also known as the Barcelona Process, and the European Neighbourhood Policy (ENP). The two case studies - Turkey and the Palestinian territories - are exceptional due to the nature of their geopolitical position in regional and international politics. Both demonstrate different levels of state development and different degrees of monopoly over the legitimate use of force, which have contributed to the development of different EU approaches to SSR, tailored to the specific needs of the local context in which the Union is operating. Both cases also demonstrate how the EU emphasizes democracy or security depending on the respective circumstantial differences. Turkey exemplifies by and large the EU accession process of fulfilling democratic reform in return for EU membership. The Palestinian case illustrates how security demands precede democratic apertures. Together, both highlight the lack of a consistent and comprehensive EU SSR strategy, the lack of which continues to impede the emergence of a common EU foreign policy. This study makes a set of recommendations for the two case studies, and concludes with more general ones applicable to the broader Euro-Mediterranean area. These are mainly addressed to the European Commission, Council of the EU (and thus, Member States) and European Parliament. Moreover, the conclusions and recommendations included in this report could inspire the work of various advocacy groups in the fields of SSR, conflict resolution and democracy and human rights promotion given that, based on the “human security” logic, this report departs from the assumption that police reform encapsulates both modernising and democratising processes

Design of high power S-band GaN MMIC power amplifiers for WiMAX applications

This paper reports two different S band GaN MMIC PA designs for WiMAX applications. First PA has a 42.6 dBm output power with a 55%PAE @ 3.5 GHz and 16 dB small signal gain in the 3.2-3.8 GHz frequency range. When two of these MMICs were combined by using off-chip Lange Couplers, 45.3 dBm output power with a 45%PAE @3.5Ghz and 16 dB small signal gain were obtained with less than 0.2 dB gain ripple in the 3.3-3.8 GHz frequency range. © 2011 IEEE

Differential spatial repositioning of activated genes in Biomphalaria glabrata snails infected with Schistosoma mansoni

Copyright @ 2014 Arican-Goktas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.This article has been made available through the Brunel Open Access Publishing Fund.Schistosomiasis is an infectious disease infecting mammals as the definitive host and fresh water snails as the intermediate host. Understanding the molecular and biochemical relationship between the causative schistosome parasite and its hosts will be key to understanding and ultimately treating and/or eradicating the disease. There is increasing evidence that pathogens that have co-evolved with their hosts can manipulate their hosts' behaviour at various levels to augment an infection. Bacteria, for example, can induce beneficial chromatin remodelling of the host genome. We have previously shown in vitro that Biomphalaria glabrata embryonic cells co-cultured with schistosome miracidia display genes changing their nuclear location and becoming up-regulated. This also happens in vivo in live intact snails, where early exposure to miracidia also elicits non-random repositioning of genes. We reveal differences in the nuclear repositioning between the response of parasite susceptible snails as compared to resistant snails and with normal or live, attenuated parasites. Interestingly, the stress response gene heat shock protein (Hsp) 70 is only repositioned and then up-regulated in susceptible snails with the normal parasite. This movement and change in gene expression seems to be controlled by the parasite. Other differences in the behaviour of genes support the view that some genes are responding to tissue damage, for example the ferritin genes move and are up-regulated whether the snails are either susceptible or resistant and upon exposure to either normal or attenuated parasite. This is the first time host genome reorganisation has been seen in a parasitic host and only the second time for any pathogen. We believe that the parasite elicits a spatio-epigenetic reorganisation of the host genome to induce favourable gene expression for itself and this might represent a fundamental mechanism present in the human host infected with schistosome cercariae as well as in other host-pathogen relationships.NIH and Sandler Borroughs Wellcome Travel Fellowshi

Design of multi-octave band GaN-HEMT power amplifier

This paper describes design, fabrication and measurement of 6 GHz - 18 GHz monolithic microwave integrated circuit (MMIC) amplifier. The amplifier is realized as coplanar waveguide (CPW) circuit using 0.3 μm-gate Gallium-Nitride (GaN) HEMT technology. The amplifier has a small signal gain of 7 ± 0.75 dB. The output power at 3dB compression is better than 24 dBm with 16%-19% drain efficiency for the whole 6 GHz-18 GHz frequency band under continuous wave (CW) power. © 2012 IEEE

Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine: Brussels, Belgium. 15-18 March 2016.

[This corrects the article DOI: 10.1186/s13054-016-1208-6.]

Targeting of human interleukin-12B by small hairpin RNAs in xenografted psoriatic skin

<p>Abstract</p> <p>Background</p> <p>Psoriasis is a chronic inflammatory skin disorder that shows as erythematous and scaly lesions. The pathogenesis of psoriasis is driven by a dysregulation of the immune system which leads to an altered cytokine production. Proinflammatory cytokines that are up-regulated in psoriasis include tumor necrosis factor alpha (TNFα), interleukin-12 (IL-12), and IL-23 for which monoclonal antibodies have already been approved for clinical use. We have previously documented the therapeutic applicability of targeting TNFα mRNA for RNA interference-mediated down-regulation by anti-TNFα small hairpin RNAs (shRNAs) delivered by lentiviral vectors to xenografted psoriatic skin. The present report aims at targeting mRNA encoding the shared p40 subunit (IL-12B) of IL-12 and IL-23 by cellular transduction with lentiviral vectors encoding anti-IL12B shRNAs.</p> <p>Methods</p> <p>Effective anti-IL12B shRNAs are identified among a panel of shRNAs by potency measurements in cultured cells. The efficiency and persistency of lentiviral gene delivery to xenografted human skin are investigated by bioluminescence analysis of skin treated with lentiviral vectors encoding the luciferase gene. shRNA-expressing lentiviral vectors are intradermally injected in xenografted psoriatic skin and the effects of the treatment evaluated by clinical psoriasis scoring, by measurements of epidermal thickness, and IL-12B mRNA levels.</p> <p>Results</p> <p>Potent and persistent transgene expression following a single intradermal injection of lentiviral vectors in xenografted human skin is reported. Stable IL-12B mRNA knockdown and reduced epidermal thickness are achieved three weeks after treatment of xenografted psoriatic skin with lentivirus-encoded anti-IL12B shRNAs. These findings mimick the results obtained with anti-TNFα shRNAs but, in contrast to anti-TNFα treatment, anti-IL12B shRNAs do not ameliorate the psoriatic phenotype as evaluated by semi-quantitative clinical scoring and by immunohistological examination.</p> <p>Conclusions</p> <p>Our studies consolidate the properties of lentiviral vectors as a tool for potent gene delivery and for evaluation of mRNA targets for anti-inflammatory therapy. However, in contrast to local anti-TNFα treatment, the therapeutic potential of targeting IL-12B at the RNA level in psoriasis is questioned.</p