A Photometric Study of the Outer Halo Globular Cluster NGC 5824

Multi-wavelength CCD photometry over 21 years has been used to produce deep color-magnitude diagrams together with light curves for the variables in the Galactic globular cluster NGC 5824. Twenty-one new cluster RR Lyrae stars are identified, bringing the total to 47, of which 42 have reliable periods determined for the first time. The color-magnitude diagram is matched using BaSTI isochrones with age of $13$~Gyr. and reddening is found to be $E(B-V) = 0.15 \pm0.02$; using the period-Wesenheit relation in two colors the distance modulus is $(m-M)_0=17.45 \pm 0.07$ corresponding to a distance of 30.9 Kpc. The observations show no signs of populations that are significantly younger than the $13$~Gyr stars. The width of the red giant branch does not allow for a spread in [Fe/H] greater than $\sigma = 0.05$ dex, and there is no photometric evidence for widened or parallel sequences. The $V, c_{UBI}$ pseudo-color magnitude diagram shows a bifurcation of the red giant branch that by analogy with other clusters is interpreted as being due to differing spectral signatures of the first (75\%) and second (25\%) generations of stars whose age difference is close enough that main sequence turnoffs in the color-magnitude diagram are unresolved. The cluster main sequence is visible against the background out to a radial distance of $\sim17$ arcmin. We conclude that NGC 5824 appears to be a classical Oosterhoff Type II globular cluster, without overt signs of being a remnant of a now-disrupted dwarf galaxy.Comment: 26 pages, 15 figures, 4 tables, accepted for publication in Astronomical Journa

Multiplex staining depicts the immune infiltrate in colitis-induced colon cancer model

Assessment of the host immune response pattern is of increasing importance as highly prognostic and diagnostic, in immune-related diseases and in some types of cancer. Chronic inflammation is a major hallmark in colon cancer formation, but, despite the extent of local inflammatory infiltrate has been demonstrated to be extremely informative, its evaluation is not routinely assessed due to the complexity and limitations of classical immunohistochemistry (IHC). In the last years, technological advance helped in bypassing technical limits, setting up multiplex IHC (mIHC) based on tyramide signal amplification (TSA) method and designing software suited to aid pathologists in cell scoring analysis. Several studies verified the efficacy of this method, but they were restricted to the analysis of human samples. In the era of translational medicine the use of animal models to depict human pathologies, in a more complete and complex approach, is really crucial. Nevertheless, the optimization and validation of this method to species other than human is still poor. We took advantage of Multispectral Imaging System to identify the immunoprofile of Dextran Sulphate Sodium (DSS)-treated mouse colon. We optimized a protocol to sequentially stain formalin fixed paraffin embedded murine colon samples for CD3, CD8a, CD4, and CD4R5B0 antigens. With this approach we obtained a detailed lymphocyte profile, while preserving the morphological tissue context, generally lost with techniques like gene expression profiling or flow cytometry. This study, comparing the results obtained by mIHC with immunophenotyping performed with cytofluorimetric and standard IHC methods validates the potentiality and the applicability of this innovative approach

Variable Stars in Local Group Galaxies. IV. RR Lyrae stars in the central regions of the low-density galaxy Crater II

We present a search and analysis of variable stars in the recently discovered Crater~II dwarf galaxy. Based on $B$, $V$, $I$ data collected with the Isaac Newton Telescope (FoV$\sim$0.44 square degrees) we detected 37 variable stars, of which 34 are bone-fide RR Lyrae stars of Crater~II (28 RRab, 4 RRc, 2 RRd). We applied the metal-independent ($V$, $B-V$) Period--Wesenheit relation and derived a true distance modulus ($\mu$ = 20.30$\pm$0.08 mag ($\sigma$=0.16 mag). Individual metallicities for RR Lyrae stars were derived by inversion of the predicted $I$-band Period-Luminosity relation. We find a mean metallicity of [Fe/H]=-1.64 and a standard deviation of $\sigma_{[Fe/H]}$ =0.21 dex, compatible with either negligible or vanishing intrinsic metallicity dispersion. The analysis of the Colour-Magnitude Diagram reveals a stark paucity of blue horizontal branch stars, at odds with other Galactic dwarfs, and globular clusters with similar metal abundances.Comment: 14 pages, 8 figures, accepted for publications on MNRAS. Time series photometry is available in the manuscript source ta

Structural parameters of star clusters: relations among light, mass and star-count radial profiles and the dependence on photometric depth

Structural parameters of model star clusters are measured in radial profiles built from number-density, mass-density and surface-brightness distributions, assuming as well different photometric conditions. Star clusters of different ages, structure and mass functions are modelled by assuming that the radial distribution of stars follows a pre-defined analytical form. Near-infrared surface brightness and mass-density profiles result from mass-luminosity relations taken from a set of isochrones. Core, tidal and half-light, half-mass and half-star count radii, together with the concentration parameter, are measured in the three types of profiles, which are built under different photometric depths. While surface-brightness profiles are almost insensitive to photometric depth, radii measured in number-density and mass-density profiles change significantly with it. Compared to radii derived with deep photometry, shallow profiles result in lower values. This effect increases for younger ages. Radial profiles of clusters with a spatially-uniform mass function produce radii that do not depend on depth. With deep photometry, number-density profiles yield radii systematically larger than those derived from surface-brightness ones. In general, low-noise surface-brightness profiles result in uniform structural parameters that are essentially independent of photometric depth. For less-populous star clusters, those projected against dense fields and/or distant ones, which result in noisy surface-brightness profiles, this work provides a quantitative way to estimate the intrinsic radii by means of number-density profiles built with depth-limited photometry.Comment: 10 pages and 9 figures. Accepted by A&

The distance to the LMC cluster Reticulum from the K-band Period-Luminosity-Metallicity relation of RR Lyrae stars

We present new and accurate Near-Infrared J and Ks-band data of the Large Magellanic Cloud cluster Reticulum. Data were collected with SOFI available at NTT and covering an area of approximately (5 x 5) arcmin^2 around the center of the cluster. Current data allowed us to derive accurate mean K-band magnitudes for 21 fundamental and 9 first overtone RR Lyrae stars. On the basis of the semi-empirical K-band Period-Luminosity-Metallicity relation we have recently derived, we find that the absolute distance to this cluster is 18.52 +- 0.005 (random) +- 0.117 (systematic). Note that the current error budget is dominated by systematic uncertainty affecting the absolute zero-point calibration and the metallicity scale.Comment: 14 pages, 2 figures, ApJ accepted. Full resolution figure 1 on request ([email protected]

Role of extracellular matrix in gastrointestinal cancer-associated angiogenesis

Gastrointestinal tumors are responsible for more cancer-related fatalities than any other type of tumors, and colorectal and gastric malignancies account for a large part of these diseases. Thus, there is an urgent need to develop new therapeutic approaches to improve the patients\u2019 outcome and the tumor microenvironment is a promising arena for the development of such treatments. In fact, the nature of the microenvironment in the different gastrointestinal tracts may significantly influence not only tumor development but also the therapy response. In particular, an important microenvironmental component and a potential therapeutic target is the vasculature. In this context, the extracellular matrix is a key component exerting an active effect in all the hallmarks of cancer, including angiogenesis. Here, we summarized the current knowledge on the role of extracellular matrix in affecting endothelial cell function and intratumoral vascularization in the context of colorectal and gastric cancer. The extracellular matrix acts both directly on endothelial cells and indirectly through its remodeling and the consequent release of growth factors. We envision that a deeper understanding of the role of extracellular matrix and of its remodeling during cancer progression is of chief importance for the development of new, more efficacious, targeted therapies

Radial velocities and membership of stars in the old, distant open cluster Berkeley 29

Multi slit spectroscopy at the Telescopio Nazionale Galileo was employed to measure radial velocities for 20 stars in the direction of the old open cluster Berkeley 29, the farthest known in our Galaxy. Membership information was derived for stars along all the red giant branch, in particular near its tip, and on the red clump. The sample of bona-fide cluster members was used to revise the cluster distance to about 15 kpc, on the basis of an empirical comparison with the red clump in open clusters with known distances. A metallicity [Fe/H] = -0.74 +/- 0.18 was also estimated using the colours of spectroscopically confirmed red giant stars.Comment: 7 pages, 5 figures (fig. 1 at low-res, but JPEG version included too), accepted for publication in A&

Stellar Population Astrophysics (SPA) with TNG. The old open clusters Collinder 350, Gulliver 51, NGC 7044, and Ruprecht 171

In the framework of the Stellar Population Astrophysics (SPA) project, we present new observations and spectral analysis of four sparsely studied open clusters, namely Collinder 350, Gulliver 51, NGC 7044, and Ruprecht 171. We exploit the HARPS-N spectrograph at the TNG telescope to acquire high-resolution optical spectra for 15 member stars of four clusters. We derive stellar parameters using both the EW analysis and the spectral fitting technique. We compute elemental abundances for light, a-, iron-peak, and n-capture elements using the EW measurement approach. We investigate the origin of the correlation between metallicity and stellar parameters derived with the EW method for the coolest stars of the sample (Teff < 4300 K). The correlation is likely due to the challenging continuum setting and to a general inaccuracy of model atmospheres used to reproduce the conditions of very cool giant stars. We locate the properties of our clusters in the radial distributions of metallicity and abundance ratios, comparing our results with clusters from the Gaia-ESO and APOGEE surveys. We present the [X/Fe]-[Fe/H] and [X/Fe]-Rgc trends for elements in common between the two surveys and derive the C and Li abundances as a function of the evolutionary phase and compare them with theoretical models. The SPA survey allows us to fully characterise the chemistry of nearby clusters. With a single set of spectra, we provide chemical abundances for some chemical elements, which are comparable to those obtained in two of the largest surveys combined. The metallicities and abundance ratios of our clusters fit very well in the radial distributions defined by the recent literature, reinforcing the importance of star clusters to outline the spatial distribution of abundances in our Galaxy. Moreover, the abundances of C and Li agree with evolutionary prescriptions for their masses and metallicities.Comment: 17 pages, 10 figures, Accepted by A&