Assessing citizen science participation skill for altruism or university course credit: a case study analysis

peer-reviewedA common challenge in citizen science projects is gaining and retaining participants. At the same time, the tertiary education sector is constantly being challenged to provide more meaningful and practical work for students. Can participation in citizen science projects be used as coursework with real practical experiential-learning benefits, without affecting the citizen science project outcomes? We seek to begin to answer this question via a case study analysis with Cyclone Center (CC), which asks participants to classify tropical cyclone characteristics through analysis of infrared satellite imagery. Skill of individual users has previously been shown to be obtainable once classifiers have looked at approximately 200 images using an expectation-maximisation likelihood approach. We use skill scores to determine if participation for course credit or altruism influenced skill for volunteers and students from two universities under three increasingly complex categories of classifications (eye or no eye; stronger, weaker, or the same; and which of six fundamental storm types). A bootstrap resampling approach was used to account for discrepancies between sample sizes. Overall, there is limited evidence for substantive differences in classification performance between credit awarded and altruistic participants, with only one finding of significance at <p = 0.05 (Maynooth University showing lower mean agreement with the volunteer consensus on eye vs. no-eye). There is evidence that integrating participation into a larger assessment that requires the students to show understanding of the project may reduce a low-skill student tail. Furthermore, students’ perceptions of the coursework compared to more traditional assignments were overall favourable. These findings, if replicated for other citizen science projects, open up possible avenues to more generally increasing participation in, and exploitation of, citizen science projects in the academic secto

New Technological Trends are Changing the Legal, Ethical, and Public Policy Implications of Sting Operations

It has long been held that a lawyer may not act deceitfully when working on a case by making false statements to the opposing party, especially to a person who has already retained counsel and the lawyer has already made statements directly to the opposing party. However, like all other legal doctrines, there are exceptions to the requirement that a lawyer should not engage in conduct involving dishonesty, fraud, deceit, or misrepresentation, as well as, the exceptions to the no-contact rule. Although there is only persuasive precedent in Virginia, it can be assumed that it is generally within the limits of professional ethics for a prosecutor to assist in an undercover (hereinafter “sting”) operation with law enforcement, even if those involved in the sting operation have reason to know that the unaware party has retained counsel. As technology progresses, the mechanisms used for law enforcement have greatly changed alongside everything else in society. The speed that technological advances often exceeds the speed at which laws can be enacted to address the new concerns that technological progressions introduce. Based on this notion, it is becoming more apparent in society that the authority of the state to enforce its laws is questionable in many of the fields in which the laws have yet to catch up with technology. One question the field of law raises is the extent to which a prosecutor may collaborate with law enforcement to use deceitful means online to gather information. Specifically, unaddressed is whether a prosecutor can legally and ethically assist law enforcement in acts, such as creating fictitious social media profiles and other types of deceptive means, over the internet when engaging in criminal investigations under Virginia law. Suppose John Doe, a man involved in a criminal enterprise, begins to get nervous that he is being investigated by the police and makes it clear to those around him that he has retained counsel and that his counsel will speak on his behalf. May the prosecutor speak directly to him? May the prosecutor ask a law enforcement official to pretend to be a layperson who wants to buy something illegal from John Doe? May a law enforcement official working with the prosecutor make a Facebook account under a fake name and then try to buy something illegal from John Doe? This analysis aims to address some of the concerns that correlate with these questions. With technology changing, the methods of law enforcement inevitably change too. Those changes result in ethical, constitutional, and civil issues that the law has yet to address to a significant extent. This analysis will first discuss the current standards conveying the ethical and legal bounds of prosecutors using deceitful acts and trickery for legitimate law enforcement purposes. It will next analyze the general legal and ethical implications of prosecutors’ involvement with the use of deceptive means or sting operations in the pre-indictment stage against a party known to already have retained counsel. The general concepts analyzed here are (a) how prosecutors can avoid Sixth Amendment concerns when dealing with parties who have already retained counsel, and (b) the reasoning as to why both federal and state courts encourage prosecutors to use sting operations. Lastly, the analysis will delve into how the application of legal and ethical concepts affects the current technological trends used by law enforcement officers for certain investigatory procedures over social media platforms

Anthropogenic Impacts on Meiosis in Plants

As the human population grows and continues to encroach on the natural environment, organisms that form part of such ecosystems are becoming increasingly exposed to exogenous anthropogenic factors capable of changing their meiotic landscape. Meiotic recombination generates much of the genetic variation in sexually reproducing species and is known to be a highly conserved pathway. Environmental stresses, such as variations in temperature, have long been known to change the pattern of recombination in both model and crop plants, but there are other factors capable of causing genome damage, infertility and meiotic abnormalities. Our agrarian expansion and our increasing usage of agrochemicals unintentionally affect plants via groundwater contamination or spray drift; our industrial developments release heavy metals into the environment; pathogens are spread by climate change and a globally mobile population; imperfect waste treatment plants are unable to remove chemical and pharmaceutical residues from sewage leading to the release of xenobiotics, all with potentially deleterious meiotic effects. In this review, we discuss the major classes of exogenous anthropogenic factors known to affect meiosis in plants, namely environmental stresses, agricultural inputs, heavy metals, pharmaceuticals and pathogens. The possible evolutionary fate of plants thrust into their new anthropogenically imposed environments are also considered

Natural and artificial sources of genetic variation used in crop breeding:A baseline comparator for genome editing

Traditional breeding has successfully selected beneficial traits for food, feed, and fibre crops over the last several thousand years. The last century has seen significant technological advancements particularly in marker assisted selection and the generation of induced genetic variation, including over the last few decades, through mutation breeding, genetic modification, and genome editing. While regulatory frameworks for traditional varietal development and for genetic modification with transgenes are broadly established, those for genome editing are lacking or are still evolving in many regions. In particular, the lack of “foreign” recombinant DNA in genome edited plants and that the resulting SNPs or INDELs are indistinguishable from those seen in traditional breeding has challenged development of new legislation. Where products of genome editing and other novel breeding technologies possess no transgenes and could have been generated via traditional methods, we argue that it is logical and proportionate to apply equivalent legislative oversight that already exists for traditional breeding and novel foods. This review analyses the types and the scale of spontaneous and induced genetic variation that can be selected during traditional plant breeding activities. It provides a base line from which to judge whether genetic changes brought about by techniques of genome editing or other reverse genetic methods are indeed comparable to those routinely found using traditional methods of plant breeding

An Analysis of Social Behaviors in Stressful Situations Using Q-Sort Data

Theories suggest that humans may respond to stressful situations by engaging in certain social behaviors aimed at minimizing the effects of stress. Though these social responses during stress have been investigated in many ways, this study adds to the literature by using a standardized Q-sort technique to examine them. When participants characterized situations as more stressful, they reported a greater tendency to distance themselves from others and lesser expressions of warmth. For female participants only, when situations were described as more anxiety-inducing, participants reported greater seeking of assurance. Though male and female participants did not differ with respect to their reports of the stressfulness of situations, they did differ with respect to their reported behaviors in those situations, with female participants reporting more expressing of warmth and male participants reporting more distancing from others.Faculty Sponsor: Diane B. V. Bonfigli

High Resolution Analysis of Meiotic Chromosome Structure and Behaviour in Barley (Hordeum vulgare L.)

Reciprocal crossing over and independent assortment of chromosomes during meiosis generate most of the genetic variation in sexually reproducing organisms. In barley, crossovers are confined primarily to distal regions of the chromosomes, which means that a substantial proportion of the genes of this crop rarely, if ever, engage in recombination events. There is potentially much to be gained by redistributing crossovers to more proximal regions, but our ability to achieve this is dependent upon a far better understanding of meiosis in this species. This study explores the meiotic process by describing with unprecedented resolution the early behaviour of chromosomal domains, the progression of synapsis and the structure of the synaptonemal complex (SC). Using a combination of molecular cytogenetics and advanced fluorescence imaging, we show for the first time in this species that non-homologous centromeres are coupled prior to synapsis. We demonstrate that at early meiotic prophase the loading of the SC-associated structural protein ASY1, the cluster of telomeres, and distal synaptic initiation sites occupy the same polarised region of the nucleus. Through the use of advanced 3D image analysis, we show that synapsis is driven predominantly from the telomeres, and that new synaptic initiation sites arise during zygotene. In addition, we identified two different SC configurations through the use of super-resolution 3D structured illumination microscopy (3D-SIM)

Licensing MLH1 sites for crossover during meiosis

During meiosis, homologous chromosomes synapse and recombine at sites marked by the binding of the mismatch repair protein MLH1. In hexaploid wheat, the Ph1 locus has a major effect on whether crossover occurs between homologues or between related homoeologues. Here we report that—in wheat–rye hybrids where homologues are absent—Ph1 affects neither the level of synapsis nor the number of MLH1. Thus in the case of wheat–wild relative hybrids, Ph1 must affect whether MLH1 sites are able to progress to crossover. The observed level of synapsis implies that Ph1 functions to promote homologue pairing rather than suppress homoeologue pairing in wheat. Therefore, Ph1 stabilises polyploidy in wheat by both promoting homologue pairing and preventing MLH1 sites from becoming crossovers on paired homoeologues during meiosis

A cytological approach to study meiotic recombination and chromosome dynamics of Arabidopsis thaliana male meiocytes in three dimensions

During meiotic prophase I chromosomes undergo dramatic conformational changes that accompany chromosome condensation, pairing and recombination between homologs. These changes include the anchoring of telomeres to the nuclear envelope and their clustering to form a bouquet. In plants, these events have been studied and illustrated in intact meiocytes of large genome species. Arabidopsis thaliana is an excellent genetic model where major molecular pathways that control synapsis and recombination between homologs have been uncovered. Yet the study of chromosome dynamics is hampered by current cytological methods that disrupt the 3D architecture of the nucleus. Here we set up a protocol to preserve the 3D configuration of A. thaliana meiocytes. We showed that this technique is compatible with the use of a variety of antibodies that label structural and recombination proteins and were able to highlight the presence of clustered synapsis initiation centers at the nuclear periphery. By using fluorescence in situ hybridization (FISH) we also studied chromosome behavior during premeiotic G2 and prophase I, revealing the existence of a telomere bouquet during A. thaliana male meiosis. In addition we showed that the number of telomeres in a bouquet and its volume vary greatly thus revealing the complexity of telomere behavior during meiotic prophase I. Finally, by using probes that label subtelomeric regions of individual chromosomes we revealed differential localization behaviors of chromosome ends. Our protocol opens new areas of research to investigate chromosome dynamics in A. thaliana meiocytesPeer reviewe