The Progenitors of Recent Core-Collapse Supernovae

We present the results of our analysis of Hubble Space Telescope (HST) and deep ground-based images to isolate the massive progenitor stars of the two recent core-collapse supernovae 2008 bk and 2008 cn. The identification of the progenitors is facilitated in one of these two cases by high-precision astrometry based on our HST imaging of SNe at late times

Detection Of DNA Damage By Use Of Escherichia Coli Carrying recA\u27::lux, uvrA\u27::lux, And alkA\u27::lux Reporter Plasmids

Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response to DNA-damaging agents, such as mitomycin and UV irradiation. Bioluminescence was measured in real time over extended periods. The fusion of the recA promoter to luxCDABE showed the most dramatic and sensitive responses. lexA dependence of the bioluminescent SOS response was demonstrated, confirming that this biosensor\u27s reports were transmitted by the expected regulatory circuitry. Comparisons were made between luxCDABE and lacZ fusions to each promoter. It is suggested that the lux biosensors may have use in monitoring chemical, physical, and genotoxic agents as well as in further characterizing the mechanisms of DNA repair

Oxidative Stress Detection With Escherichia Coli Harboring A katG\u27::lux Fusion

A plasmid containing a transcriptional fusion of the Escherichia coli katG promoter to a truncated Vibrio fischeri lux operon (luxCDABE) was constructed. An E. coli strain bearing this plasmid (strain DPD2511) exhibited low basal levels of luminescence, which increased up to 1,000-fold in the presence of hydrogen peroxide, organic peroxides, redox-cycling agents (methyl viologen and menadione), a hydrogen peroxide-producing enzyme system (xanthine and xanthine oxidase), and cigarette smoke. An oxyR deletion abolished hydrogen peroxide-dependent induction, confirming that oxyR controlled katG\u27::lux luminescence. Light emission was also induced by ethanol by an unexplained mechanism. A marked synergistic response was observed when cells were exposed to both ethanol and hydrogen peroxide; the level of luminescence measured in the presence of both inducers was much higher than the sum of the level of luminescence observed with ethanol and the level of luminescence observed with hydrogen peroxide. It is suggested that this construction or similar constructions may be used as a tool for assaying oxidant and antioxidant properties of chemicals, as a biosensor for environmental monitoring and as a tool for studying cellular responses to oxidative hazards

Bayesian Analysis of Two Stellar Populations in Galactic Globular Clusters II: NGC 5024, NGC 5272, and NGC 6352

We use Cycle 21 Hubble Space Telescope (HST) observations and HST archival ACS Treasury observations of Galactic Globular Clusters to find and characterize two stellar populations in NGC 5024 (M53), NGC 5272 (M3), and NGC 6352. For these three clusters, both single and double-population analyses are used to determine a best fit isochrone(s). We employ a sophisticated Bayesian analysis technique to simultaneously fit the cluster parameters (age, distance, absorption, and metallicity) that characterize each cluster. For the two-population analysis, unique population level helium values are also fit to each distinct population of the cluster and the relative proportions of the populations are determined. We find differences in helium ranging from $\sim$0.05 to 0.11 for these three clusters. Model grids with solar $\alpha$-element abundances ([$\alpha$/Fe] =0.0) and enhanced $\alpha$-elements ([$\alpha$/Fe]=0.4) are adopted.Comment: ApJ, 21 pages, 14 figures, 7 table

Radio Detection of SN 1986E in NGC 4302

Radio observations of SN 1986E have shown a clear detection of emission at 6 cm wavelength about 8 months after optical discovery. Combined with a number of new upper limits and a study of the possible models, it appears that SN 1986E was probably a fairly normal Type IIL supernova, somewhat similar to SN 1980K, with radio emission at roughly expected levels. This detection continues the correlation between radio detection and late time optical emission.Comment: 14 pages, LaTeX (AASTeX), 2 PostScript figures, to appear in ApJ (Letters

BAYESIAN ANALYSIS OF TWO STELLAR POPULATIONS IN GALACTIC GLOBULAR CLUSTERS I: STATISTICAL AND COMPUTATIONAL METHODS

We develop a Bayesian model for globular clusters composed of multiple stellar populations, extending earlier statistical models for open clusters composed of simple (single) stellar populations (e.g., van Dyk et al. 2009; Stein et al. 2013). Specifically, we model globular clusters with two populations that differ in helium abundance. Our model assumes a hierarchical structuring of the parameters in which physical properties—age, metallicity, helium abundance, distance, absorption, and initial mass—are common to (i) the cluster as a whole or to (ii) individual populations within a cluster, or are unique to (iii) individual stars. An adaptive Markov chain Monte Carlo (MCMC) algorithm is devised for model fitting that greatly improves convergence relative to its precursor non-adaptive MCMC algorithm. Our model and computational tools are incorporated into an open-source software suite known as BASE-9. We use numerical studies to demonstrate that our method can recover parameters of two-population clusters, and also show model misspecification can potentially be identified. As a proof of concept, we analyze the two stellar populations of globular cluster NGC 5272 using our model and methods. (BASE-9 is available from GitHub: https://github.com/argiopetech/base/releases)