Vacuum-Induced Surface Freezing for the Freeze-Drying of the Human Growth Hormone: How Does Nucleation Control Affect Protein Stability?

Abstract In the present work, the effect of controlled nucleation on the stability of human growth hormone (hGH) during freeze-drying has been investigated. More specifically, the vacuum-induced surface freezing technique has been compared to conventional freezing, both with and without an annealing step. Size exclusion chromatography and cell-based potency assays have been used to characterize the formation of soluble aggregates and the biological activity of hGH, respectively. The results obtained indicate that controlled nucleation has a positive effect on both cycle performance and product homogeneity because of the formation of bigger ice crystals, and characterized by a narrower dimensional distribution. From the point of view of hGH stability, we observed that vacuum-induced surface freezing is not detrimental to the biological activity of the protein, or aggregate formation. In addition, the effect of 2 different formulations, including trehalose or cellobiose, on protein preservation was also considered for this study

Consequences of long-term consumption of water from Nworie River (Owerri, Nigeria) on haematological, hepatic, and renal functions using rat model

The consequences of long term consumption of water from Nworie River (Owerri, Nigeria) on haematological, hepatic, and renal functions using rat model (Wistar albino strain). Twenty-four rats separated into two groups of twelve rats each were kept as test and control for sixty-four days. The test rats were placed on water from Nworie River while those of the control were placed on Eva water (purified Coca-cola bottled water). The rats were sacrificed in two sets: first set was on thirty-second day while the second set was on the sixty-fourth day. Six rats each from each group were sacrificed at each set. The Results obtained revealed that Hb, PCV, WBC, L, N, and ESR were significantly affected (p<0.05) in test rats against the control rats. The functional parameters of liver adequacy; AST, ALT, and ALP were significantly (p<0.05) affected in test rats against those of the control. Also, urea and electrolyte ions (Potassium ion, chloride and bicarbonate) indicating renal sufficiency were significantly (p<0.05) affected in test rats against those of the control. Creatinine, sodium ion, total bilirubin and conjugated bilirubin were not significantly affected (p>0.05) in test rats when compared to those of the control. The induced changes in the parameters investigated in this study have shown that long-term consumption of water from Nworie River has effect on haematological, hepatic, and renal function

Extraction of isoflavones from different raw sources using natural deep eutectic solvents

This research was partially supported by RFBR 17-03-00641 and the Ministry of Education of the Russian Federation within the framework of the basic part of the state task, Project No. 4.9514.2017 / 8.9

The cytotoxic evaluation and regenerative potentials of isoflavones in diabetic animal models

The present study evaluated the antidiabetic effect of daidzein based on its ability to lower blood glucose, and influence the regenerative mechanism of pancreatic β-cell in type 1 diabetic animal models. Furthermore, the cytotoxic effect of this isoflavone at dose of 100mg/kg and 200mg/kg of animal body weight was evaluated in the kidney

Macroscopic Quantum Tunneling of a Bose Condensate

We study, by means of a variational method, the stability of a condensate in a magnetically trapped atomic Bose gas with a negative scattering length and find that the condensate is unstable in general. However, for temperatures sufficiently close to the critical temperature the condensate turns out to be metastable. For that case we determine in the usual WKB approximation the decay rate of the condensate due to macroscopic quantum fluctuations. When appropriate, we also calculate the decay rate due to thermal fluctuations. An important feature of our approach is that (nonsingular) phase fluctuations of the condensate are taken into account exactly.Comment: Invited paper for the Journal of Statistical Physic

Exact expression for the diffusion propagator in a family of time-dependent anharmonic potentials

We have obtained the exact expression of the diffusion propagator in the time-dependent anharmonic potential $V(x,t)={1/2}a(t)x^2+b\ln x$. The underlying Euclidean metric of the problem allows us to obtain analytical solutions for a whole family of the elastic parameter a(t), exploiting the relation between the path integral representation of the short time propagator and the modified Bessel functions. We have also analyzed the conditions for the appearance of a non-zero flow of particles through the infinite barrier located at the origin (b<0).Comment: RevTex, 19 pgs. Accepted in Physical Review

DIFFUSION IN ONE DIMENSIONAL RANDOM MEDIUM AND HYPERBOLIC BROWNIAN MOTION

Classical diffusion in a random medium involves an exponential functional of Brownian motion. This functional also appears in the study of Brownian diffusion on a Riemann surface of constant negative curvature. We analyse in detail this relationship and study various distributions using stochastic calculus and functional integration.Comment: 18 page

Boundary Shape and Casimir Energy

Casimir energy changes are investigated for geometries obtained by small but arbitrary deformations of a given geometry for which the vacuum energy is already known for the massless scalar field. As a specific case, deformation of a spherical shell is studied. From the deformation of the sphere we show that the Casimir energy is a decreasing function of the surface to volume ratio. The decreasing rate is higher for less smooth deformations.Comment: 12 page