Prevalence and Pattern of Adverse Drug Reactions among HIV Infected Patients on Highly Active Antiretroviral Therapy (HAART) in Imo State, Nigeria: A Hospital Based Survey

Introduction:  It is a proven fact that almost all drugs carry the potential to produce undesirable effects in addition to the desired ones. Aim:  The aim of this study is to determine the prevalence, pattern and socio-demographic determinants of adverse drug reactions among patients on HAART attending clinics in hospitals in Imo State Nigeria. Methodology:  This was a hospital-based cross-sectional study carried out among HIV patients attending clinics in hospitals in Imo State.  The 400 participants were selected using a multistage sampling technique.  Data was collected using an interviewer administered, semi-structured questionnaire and analyzed using EPI Info version 3:2:1. Chi-square and regression analysis was used to test association between variables. P-value was set at ≤ 0.05. Results: The mean age of the participants was 41.7±3.0 years with a male to female ration of 1:2. The adherence level to HIV treatment was 85.0%. The prevalence of ADRs in the last 1 year preceding the study was 13.7% and the common forms of ADR experienced by patients was Hematological symptoms (34%), GIT symptoms (21.8%) and skin manifestation (20.0%). Socio-demographic determinants of the occurrence of ADR among the participants were: age 50 years and above (OR: 9.28), female gender (OR: 2.55) and living in a rural area (OR: 4.47). Conclusion:  Though the prevalence of ADR reported in this study was low, there is need to increase the depth of knowledge among HIV patients in the State, monitor patients closely by care givers and find possible ways to increase adherence to HIV drugs which was not optimal among participants. Keywords: Prevalence, Pattern, Adverse Drug reactions, HAART, HIV, Patients, Imo State

Cyclin B1/Cdk1 phosphorylation of mitochondrial p53 induces anti-apoptotic response.

The pro-apoptotic function of p53 has been well defined in preventing genomic instability and cell transformation. However, the intriguing fact that p53 contributes to a pro-survival advantage of tumor cells under DNA damage conditions raises a critical question in radiation therapy for the 50% human cancers with intact p53 function. Herein, we reveal an anti-apoptotic role of mitochondrial p53 regulated by the cell cycle complex cyclin B1/Cdk1 in irradiated human colon cancer HCT116 cells with p53(+/+) status. Steady-state levels of p53 and cyclin B1/Cdk1 were identified in the mitochondria of many human and mouse cells, and their mitochondrial influx was significantly enhanced by radiation. The mitochondrial kinase activity of cyclin B1/Cdk1 was found to specifically phosphorylate p53 at Ser-315 residue, leading to enhanced mitochondrial ATP production and reduced mitochondrial apoptosis. The improved mitochondrial function can be blocked by transfection of mutant p53 Ser-315-Ala, or by siRNA knockdown of cyclin B1 and Cdk1 genes. Enforced translocation of cyclin B1 and Cdk1 into mitochondria with a mitochondrial-targeting-peptide increased levels of Ser-315 phosphorylation on mitochondrial p53, improved ATP production and decreased apoptosis by sequestering p53 from binding to Bcl-2 and Bcl-xL. Furthermore, reconstitution of wild-type p53 in p53-deficient HCT116 p53(-/-) cells resulted in an increased mitochondrial ATP production and suppression of apoptosis. Such phenomena were absent in the p53-deficient HCT116 p53(-/-) cells reconstituted with the mutant p53. These results demonstrate a unique anti-apoptotic function of mitochondrial p53 regulated by cyclin B1/Cdk1-mediated Ser-315 phosphorylation in p53-wild-type tumor cells, which may provide insights for improving the efficacy of anti-cancer therapy, especially for tumors that retain p53

EFFICACY OF XYLOPIA AETHIOPICA (DUNAL) A. AND AFRAMOMUM MELEGUETA (ROSC) K. SCHUM. FRUIT ON SITOPHULIUS ZEA

The power of combined powder of dried fruits of Xylopia aethiopica(Dunal) A. and Aframomum melegueta on the control of maize weevil Sitophulus zea were evaluated. The aim was to determine, their antifeedant potentials against the insect pests of stored maize. Oven dried fruits of X. aethiopica and A. melegueta was pulverized and mixed in equal proportion. Concentrations of 1,2,3,4,5,6,7,8,9,and10g were mixed with 100g of healthy grain, and incubated for 14 days with 20 weevils in each. Each treatment plus the control (0.0g) were replicated five times and laid out randomly. Results showed that mortality and % mortality of weevils increased progressively with the powder concentrations and the exposure period. Within the 14 days of exposure, mortality and % mortality were 4, 20% in the control and all, 100% in the test treatments. Reduction in seed damage increased significantly with the powder concentration (P<0.05). Weevil perforation index (WPI) showed that the combined powder had high protectant activity and therefore could serve as an alternative to the conventional chemical insect pesticides. Key Words: Xylopia aethiopica,  Aframomum  melegueta,  Sitophulus  zea,  combined powder

Pattern and outcome of admissions as seen in the paediatric emergency ward of the Niger Delta University Teaching Hospital Bayelsa State, Nigeria

Objective: To describe the pattern and outcome of childhood illnesses seen in a paediatric emergency ward of a tertiary centre in the Niger Delta region of Nigeria.Methods: Admission records of all children seen in the Children’s Emergency Ward (CHEW) between the 1st of January 2008 and 31st ofDecember 2011 were retrospectively reviewed and analysed.Results: A total of 1756 children were admitted into the emergency ward over a four year period (1st January 2008 to 31st December 2011). The age range was one month to 18 years with a mean of 36.6 months. There was a male preponderance with a male to female ratio of 1.4:1. Majority, 1386 (78.9%) of the patients were below the age of five years. The meanduration of stay was 2.2 days. The major causes of admission were malaria, 562 (32.0%), diarrhoeal disease 389 (22.2%), respiratory tract infections 162 (9.2%) and anaemic heart failure 112(6.4%). Peak admissions period and mortality were in the months of January, May and December. There were 799 transfers, 710 discharges, 94 discharges against medical advice and 20 referrals. Over the period, 133 children diedgiving a mortality rate of 7.6%. Major causes of mortality wereanaemic heart failure 32(24.1%), malaria 26 (19.6%), septicaemia 17(12.8%) and diarrhoeal disease 15 (11.3%).Conclusions: Infections are the major cause of morbidity and mortalityin the study environment with the under-fives being the most vulnerable.Key words: children, under-fives, emergency room, admissions, morbidity, mortalit

Protein enrichment of solid waste from cocoyam (Xanthosoma sagittifolium (L.) Schott) cormel processing using Aspergillus oryzae obtained from cormel flour

Aspergillus oryzae obtained from spoilt cormel flour was subjected to mutation treatments using X-rays, solar radiation and bleach. Following selection and screening of viable colonies on a medium containing Xanthosoma cormel solid process waste as the only carbon source, A. oryzae A7 which significantly (p < 0.05) produced more biomass at a higher growth rate than the wild parent, was chosen for protein enrichment. Protein content of substrate enriched with the mutant fungal strain was higher than that enriched with the wild strain. Addition of (NH4) 2SO4, NH4NO3, NH4Cl, and urea to Xanthosoma solid process waste increased the growth rate of mutant, with the highest  increase observed with urea. Medium amended with urea also had the highest protein level of 26.23% strain compared to a protein yield of 17.41% obtained in the control with no added nitrogen. The optimal temperature for protein enrichment was found to be 35°C. (African Journal of Biotechnology: 2003 2(8): 228-232

Path integral for a relativistic Aharonov-Bohm-Coulomb system

The path integral for the relativistic spinless Aharonov-Bohm-Coulomb system is solved, and the energy spectra are extracted from the resulting amplitude.Comment: 6 pages, Revte

Self-regulated learning skill as a predictor of mathematics achievement: a focus on ability levels

This paper investigated self-regulated learning skills as a predictor of students’ achievement in mathematics based on ability level. The study is prediction-design research of correlational type. The subjects were 882 SSII students from the secondary schools in Owerri Education Zone of Imo State. The researchers adopted the multi-stage but simple random sampling technique to draw the sample. Two instruments were used to collect data for this study. They are the Self-regulated Learning Questionnaire (SRLQ) and Mathematics Achievement Proforma. The validity of the instruments was ensured through experts’ suggestions and guidance. Single-administration reliability and Cronbach Alpha ensured the reliability of SRLQ (0.89). The data collected were analyzed using regression analysis and coefficient of determination at 0.05 alpha level with the aid of Statistical Package for Social Sciences (SPSS) version 20. The results of the study revealed that self-regulated learning skill predicts 6.0% and 4.3% respectively to the variance observed in high and low achieving students in mathematics. This prediction is significant as attested to by the regression analysis carried out (p < 0.05). Therefore, secondary school students should regulate their learning to increase their mathematics achievement

Use of Remote Sensing Data to Detect Environmental Degradation in the Oil Rich Region of Southern Nigeria between 2003 and 2015

The oil spill management system aims to achieve a knowledge- based system which can choose the most suitable method of response in shorter time by analyzing the various sensitivity factors of coastal environment, affecting parameters on oil spill movement, environmental concerns in oil spill response, and consequent monitoring and clean-up measurements. The major advantage of this integration is the ability to extract oil spill parameters such as location, linear size and spill areas. Spatial and temporal information, i.e. oil spill distribution at the sea, its frequency and evolution in time allow the scientists to establish the major cause and source of oil spills, and then outline the risk areas. This study has demonstrated the application of GIS and remote sensing as a decision support tool for oil spill management. Its objectives are to perform image classification and accuracy assessment, to perform post classification change detection for oil spill detection and to perform trend of change analysis for oil spill growth trend. Methodology involves planning stage, data requirements, data acquisition, data processing and results presentation. The results indicated that the annual growth rate of water bodies is decreasing at -0.16% from 2003 to 2015, settlements decreased at a rate of -1.16% from 2003 to 2015 while Mangrove and vegetation decreased significantly at the rate of -2.82% and -1.92% respectively from 2003 to 2015, this is by far the most significant decrease in the study area, as oil spill degrades farmland and plantations there by rendering it useless for economical purposes, the results also indicated that degraded environment increased at a rate of 3.39% from 2003 to 2015. It was further recommended amongst others that further studies should be on oil spill management in Gokana L.G.A as this will provide additional information on how to manage the effects of oil spill in Gokana L.G.A

slq(2)sl_q(2) Realizations for Kepler and Oscillator Potentials and q-Canonical Transformations

The realizations of the Lie algebra corresponding to the dynamical symmetry group SO(2,1) of the Kepler and oscillator potentials are q-deformed. The q-canonical transformation connecting two realizations is given and a general definition for q-canonical transformation is deduced. q-Schr\"{o}dinger equation for a Kepler like potential is obtained from the q-oscillator Schr\"{o}dinger equation. Energy spectrum and the ground state wave function are calculated.Comment: 12 pages, Latex twice, (Comparison with the other approaches and some refs. added. The version which will appear in J. Phys. A

Alternative Solution of the Path Integral for the Radial Coulomb Problem

In this Letter I present an alternative solution of the path integral for the radial Coulomb problem which is based on a two-dimensional singular version of the Levi-Civita transformation.Comment: 7 pages, Late